Origin IGS: ttttaggttcttttgaagaaaaattgcgctaaaaataacttattttttatcatttaacaagttaccagccgatttatgtgtataattacgctgtttatttggcatttaatattttttgctgaattttttaatatttgcttggtcataaatacacttaaaccacaatatgcagtcaaggaatcaacgtc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gacgttgattccttgactgcatattgtggtttaagtgtatttatgaccaagcaaatattaaaaaattcagcaaaaaatattaaatgccaaataaacagcgtaattatacacataaatcggctggtaacttgttaaatgataaaaaataagttatttttagcgcaatttttcttcaaaagaacctaaaa Mask Tandem Repeat Region ================================================ ttttaggttcttttgaagaaaaattgcgctaaaaataacttattttttatcatttaacaagttaccagccgatttatgtgtataattacgctgtttatttggcatttaatattttttgctgaattttttaatatttgcttggtcataaatacacttaaaccacaatatgcagtcaaggaatcaacgtc Find is-nt database================================================ Query_seq: AA01820:AA01822|AA01820:AA01822:high-affinity zinc uptake system ATP-binding protein:lysostaphin precursor:->->:1226014..1226201 188 ttttaggttcttttgaagaaaaattgcgctaaaaataacttattttttatcatttaacaagttaccagccgatttatgtgtataattacgctgtttatttggcatttaatattttttgctgaattttttaatatttgcttggtcataaatacacttaaaccacaatatgcagtcaaggaatcaacgtc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01820:AA01822|AA01820:AA01822:high-affinity zinc uptake system ATP-binding protein:lysostaphin precursor:->->:1226014..1226201 188 ttttaggttcttttgaagaaaaattgcgctaaaaataacttattttttatcatttaacaagttaccagccgatttatgtgtataattacgctgtttatttggcatttaatattttttgctgaattttttaatatttgcttggtcataaatacacttaaaccacaatatgcagtcaaggaatcaacgtc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01820:AA01822|AA01820:AA01822:high-affinity zinc uptake system ATP-binding protein:lysostaphin precursor:->->:1226014..1226201 188 ttttaggttcttttgaagaaaaattgcgctaaaaataacttattttttatcatttaacaagttaccagccgatttatgtgtataattacgctgtttatttggcatttaatattttttgctgaattttttaatatttgcttggtcataaatacacttaaaccacaatatgcagtcaaggaatcaacgtc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttttaggttcttttgaagaaaaattgcgctaaaaataacttattttttatcatttaacaagttaccagccgatttatgtgtataattacgctgtttatttggcatttaatattttttgctgaattttttaatatttgcttggtcataaatacacttaaaccacaatatgcagtcaaggaatcaacgtc Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 76 End: 186 ........................................................................... M C I I T L F I W H L I F F A E F F N I C L V I N T L K P Q Y A V K E S T .. Protein_Len: 40 Strand: - Start: 57 End: 176 ........................................................ C T V L R N I H I I V S N I Q C K I N K A S N K L I Q K T M F V S L G C Y A T M ............ ttttaggttcttttgaagaaaaattgcgctaaaaataacttattttttatcatttaacaagttaccagccgatttatgtgtataattacgctgtttatttggcatttaatattttttgctgaattttttaatatttgcttggtcataaatacacttaaaccacaatatgcagtcaaggaatcaacgtc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 105 End: 134 ........................................................................................................TTTAATATTTTTTGCTGAATTTTTTAATAT...................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttttaggttcttttgaagaaaaattgcgctaaaaataacttattttttatcatttaacaagttaccagccgatttatgtgtataattacgctgtttatttggcatttaatattttttgctgaattttttaatatttgcttggtcataaatacacttaaaccacaatatgcagtcaaggaatcaacgtc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01820:AA01822|AA01820:AA01822:high-affinity zinc uptake system ATP-binding protein:lysostaphin precursor:->->:1226014..1226201 188 ttttaggttcttttgaagaaaaattgcgctaaaaataacttattttttatcatttaacaagttaccagccgatttatgtgtataattacgctgtttatttggcatttaatattttttgctgaattttttaatatttgcttggtcataaatacacttaaaccacaatatgcagtcaaggaatcaacgtc Intra-Species Hit: Count: 1 Min: 1 Max: 188 Len: 188 Subject: aact_AA01820_AA01822|high-affinity zinc uptake system ATP-binding protein:lysostaphin precursor|POSITIVE:POSITIVE|[1226014,1226201]|188 HSP 1 e-value: 9.0E-33 bit: 141.0 Len: 71 Query Start:1 Query End:71 Subject Strand: POSITIVE Subject Start: 1 Subject End: 71 ttttaggttcttttgaagaaaaattgcgctaaaaataacttattttttatcatttaacaagttaccagccg..................................................................................................................... ttttaggttcttttgaagaaaaattgcgctaaaaataacttattttttatcatttaacaagttaccagccg..................................................................................................................... HSP 2 e-value: 2.0E-21 bit: 103.0 Len: 52 Query Start:137 Query End:188 Subject Strand: POSITIVE Subject Start: 137 Subject End: 188 ........................................................................................................................................gcttggtcataaatacacttaaaccacaatatgcagtcaaggaatcaacgtc ........................................................................................................................................gcttggtcataaatacacttaaaccacaatatgcagtcaaggaatcaacgtc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUAGGUUCUUUUGAAGAAAAAUUGCGCUAAAAAUAACUUAUUUUUUAUCAUUUAACAAGUUACCAGCCGAUUUAUGUGUAUAAUUACGCUGUUUAUUUGGCAUUUAAUAUUUUUUGCUGAAUUUUUUAAUAUUUGCUUGGUCAUAAAUACACUUAAACCACAAUAUGCAGUCAAGGAAUCAACGUC .....((((((((.((.((((((((..((.(((((((....((((.(((.....))).)))).....(((((.....(((((....))))).......)))))......))))))).))..))))))))......(((.((((..(((......))).))))......))).))))))))))...... (-30.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GACGUUGAUUCCUUGACUGCAUAUUGUGGUUUAAGUGUAUUUAUGACCAAGCAAAUAUUAAAAAAUUCAGCAAAAAAUAUUAAAUGCCAAAUAAACAGCGUAAUUAUACACAUAAAUCGGCUGGUAACUUGUUAAAUGAUAAAAAAUAAGUUAUUUUUAGCGCAAUUUUUCUUCAAAAGAACCUAAAA ....((((......((.(((.......((((((.(((((((((((.....((.................))......((((........)))).....)))))..)))))).)))))).((((((((((((((...........))))))))))...))))))).)).....))))............ (-25.23) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 313 121 85 53 18 aact:AA00534|5end_hypothetical_protein_365419..365649_POSITIVE 256 96 58 221 193 aact:AA00419|5end_conserved_hypothetical_protein_(possible_phosphatidylethanolamine-binding_protein)_284337..284567_POSITIVE 238 98 56 57 12 aact:AA01473|5end_type_I_restriction-modification_system_S_(specificity_subunit)_989025..989255_POSITIVE 235 120 78 163 119 aact:AA00043|5end_formate_dependent_nitrite_reductase,_transmembrane_protein_34378..34608_POSITIVE 234 103 69 142 111 aact:AA01338|5end_hypothetical_protein_904967..905197_POSITIVE 228 148 102 131 83 aact:AA00796|5end_iron(III)_dicitrate_transport_system_permease_protein_545686..545916_POSITIVE 226 103 62 230 186 aact:AA01418|5end_conserved_hypothetical_protein_953951..954181_POSITIVE 225 180 141 73 41 aact:AA02473|5end_conserved_hypothetical_protein_1708571..1708801_POSITIVE 224 103 63 60 14 aact:AA01386|5end_30S_ribosomal_protein_S1_935974..936204_POSITIVE 222 144 102 136 93 aact:AA02681|5end_hypothetical_protein_1865749..1865979_POSITIVE 222 103 61 209 158 aact:AA01565|5end_conserved_hypothetical_protein_1050711..1050941_POSITIVE 221 99 60 212 167 aact:AA01890|5end_cell-cycle_protein_1274544..1274774_POSITIVE 219 96 58 187 147 aact:AA02826|5end_conserved_hypothetical_protein_1986336..1986566_POSITIVE 218 126 89 75 35 aact:AA00816|5end_lacZ_expression_regulator_560900..561130_POSITIVE 217 95 59 169 137 aact:AA01347|5end_conserved_hypothetical_protein_913209..913439_POSITIVE 214 98 68 199 170 aact:AA00529|5end_outer_membrane_protein,_Omp_18/16_361730..361960_POSITIVE 212 108 62 200 143 aact:AA02455|5end_conserved_hypothetical_protein_1694353..1694583_POSITIVE 212 129 87 166 123 aact:AA00540|5end_hypothetical_protein_370050..370280_POSITIVE 210 160 116 87 51 aact:AA02190|5end_hydrogenase-2_component_protein_1492919..1493149_POSITIVE 210 103 61 82 53 aact:AA01179|5end_beta-ketoacyl-ACP_reductase;_short-chain_alcohol_dehydrogenase_807996..808226_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 313 121 85 148 113 aact:AA00534|3end_hypothetical_protein_365594..365744_POSITIVE 313 121 85 76 41 aact:AA00533|3end_conserved_hypothetical_protein_365522..365672_POSITIVE 256 96 58 112 84 aact:AA00417|3end_outer_membrane_lipoprotein_precursor_(probable_D-methionine-binding_lipoprotein)_284308..284458_POSITIVE 238 98 56 110 65 aact:AA01472|3end_hypothetical_protein_989158..989308_POSITIVE 234 103 69 126 95 aact:AA01337|3end_cell_division_protein_N_905031..905181_POSITIVE 230 121 88 102 65 aact:AA02419|3end_hypothetical_protein_1661954..1662104_POSITIVE 228 148 102 128 80 aact:AA00795|3end_iron(III)_dicitrate-binding_periplasmic_protein_precursor_545763..545913_POSITIVE 225 121 86 112 72 aact:AA02476|3end_conserved_hypothetical_protein_1712817..1712967_POSITIVE 222 144 102 118 75 aact:AA02679|3end_formate_dehydrogenase,_gamma_subunit_1865811..1865961_POSITIVE 220 82 48 125 87 aact:AA00939|3end_transcription_accessory_protein_(toxin_expression)_650724..650874_POSITIVE 219 108 69 88 55 aact:AA01441|3end_tRNA/rRNA_methylase_967643..967793_POSITIVE 219 109 64 150 96 aact:AA00602|3end_1-deoxy-D-xylulose_5-phosphate_reductoisomerase_410251..410401_POSITIVE 217 95 59 141 109 aact:AA01345|3end_conserved_hypothetical_protein_(possible_membrane_protein)_913261..913411_POSITIVE 216 88 43 115 68 aact:AA01894|3end_conserved_hypothetical_protein_(possible_transthyretin-like_periplasmic_protein)_1279457..1279607_POSITIVE 214 98 68 92 63 aact:AA00528|3end_conserved_hypothetical_protein_361703..361853_POSITIVE 212 129 87 83 40 aact:AA00539|3end_ADP-L-glycero-D-manno-heptose-6-epimerase_370047..370197_POSITIVE 210 160 116 54 18 aact:AA02188|3end_hypothetical_protein_1492966..1493116_POSITIVE 210 103 61 59 30 aact:AA01177|3end_beta-ketoacyl-ACP_synthase_IV_808053..808203_POSITIVE 210 103 68 141 103 aact:AA00943|3end_conserved_hypothetical_protein_654542..654692_POSITIVE 209 102 67 35 4 aact:AA02599|3end_outer_membrane_protein,_Slp_family_1810717..1810867_POSITIVE