Origin IGS: tttgcgccacacgacaaaagcccctaatggtgcggtaatcagtgaaagaatgatgcctgtcagccaggcgggaaagagaatatcaaacataatgtcagtcgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacgactgacattatgtttgatattctctttcccgcctggctgacaggcatcattctttcactgattaccgcaccattaggggcttttgtcgtgtggcgcaaa Mask Tandem Repeat Region ================================================ tttgcgccacacgacaaaagcccctaatggtgcggtaatcagtgaaagaatgatgcctgtcagccaggcgggaaagagaatatcaaacataatgtcagtcgtta Find is-nt database================================================ Query_seq: AA01818:AA01820|AA01818:AA01820:high-affinity zinc uptake system membrane protein:high-affinity zinc uptake system ATP-binding protein:->->:1225139..1225242 104 tttgcgccacacgacaaaagcccctaatggtgcggtaatcagtgaaagaatgatgcctgtcagccaggcgggaaagagaatatcaaacataatgtcagtcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01818:AA01820|AA01818:AA01820:high-affinity zinc uptake system membrane protein:high-affinity zinc uptake system ATP-binding protein:->->:1225139..1225242 104 tttgcgccacacgacaaaagcccctaatggtgcggtaatcagtgaaagaatgatgcctgtcagccaggcgggaaagagaatatcaaacataatgtcagtcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01818:AA01820|AA01818:AA01820:high-affinity zinc uptake system membrane protein:high-affinity zinc uptake system ATP-binding protein:->->:1225139..1225242 104 tttgcgccacacgacaaaagcccctaatggtgcggtaatcagtgaaagaatgatgcctgtcagccaggcgggaaagagaatatcaaacataatgtcagtcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tttgcgccacacgacaaaagcccctaatggtgcggtaatcagtgaaagaatgatgcctgtcagccaggcgggaaagagaatatcaaacataatgtcagtcgtta Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 2 End: 103 . M R H T T K A P N G A V I S E R M M P V S Q A G K R I S N I M S V V . Protein_Len: 31 Strand: - Start: 1 End: 93 K R W V V F A G L P A T I L S L I I G T L W A P F L I D F M M ........... tttgcgccacacgacaaaagcccctaatggtgcggtaatcagtgaaagaatgatgcctgtcagccaggcgggaaagagaatatcaaacataatgtcagtcgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tttgcgccacacgacaaaagcccctaatggtgcggtaatcagtgaaagaatgatgcctgtcagccaggcgggaaagagaatatcaaacataatgtcagtcgtta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01818:AA01820|AA01818:AA01820:high-affinity zinc uptake system membrane protein:high-affinity zinc uptake system ATP-binding protein:->->:1225139..1225242 104 tttgcgccacacgacaaaagcccctaatggtgcggtaatcagtgaaagaatgatgcctgtcagccaggcgggaaagagaatatcaaacataatgtcagtcgtta Intra-Species Hit: Count: 1 Min: 1 Max: 104 Len: 104 Subject: aact_AA01818_AA01820|high-affinity zinc uptake system membrane protein:high-affinity zinc uptake system ATP-binding protein|POSITIVE:POSITIVE|[1225139,1225242]|104 HSP 1 e-value: 1.0E-52 bit: 206.0 Len: 104 Query Start:1 Query End:104 Subject Strand: POSITIVE Subject Start: 1 Subject End: 104 tttgcgccacacgacaaaagcccctaatggtgcggtaatcagtgaaagaatgatgcctgtcagccaggcgggaaagagaatatcaaacataatgtcagtcgtta tttgcgccacacgacaaaagcccctaatggtgcggtaatcagtgaaagaatgatgcctgtcagccaggcgggaaagagaatatcaaacataatgtcagtcgtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUGCGCCACACGACAAAAGCCCCUAAUGGUGCGGUAAUCAGUGAAAGAAUGAUGCCUGUCAGCCAGGCGGGAAAGAGAAUAUCAAACAUAAUGUCAGUCGUUA ..........(((((......(((...((((((((..((((.........))))..))))..))))...)))...((.....)).............))))).. (-22.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACGACUGACAUUAUGUUUGAUAUUCUCUUUCCCGCCUGGCUGACAGGCAUCAUUCUUUCACUGAUUACCGCACCAUUAGGGGCUUUUGUCGUGUGGCGCAAA ....((.(((.((((....))))............(((((.....))))).))).))..............((.((((..(.(((....))).))))).))... (-21.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 357 44 3 125 74 aact:AA02076|5end_conserved_hypothetical_protein_1409926..1410156_POSITIVE 351 78 33 150 106 aact:AA00286|5end_glutamate_5-kinase_(gamma-glutamyl_kinase)_199965..200195_POSITIVE 328 70 22 143 89 aact:AA01056|5end_conserved_hypothetical_protein_733867..734097_POSITIVE 327 77 31 181 140 aact:AA00343|5end_conserved_hypothetical_protein_(possible_cytosolic_protein)_229908..230138_POSITIVE 325 48 4 71 30 aact:AA00190|5end_triosephosphate_isomerase_134088..134318_POSITIVE 319 44 9 48 12 aact:AA01807|5end_electron_transport_complex_protein_1216696..1216926_POSITIVE 319 79 31 74 9 aact:AA01515|5end_SlyX_family_protein_1020682..1020912_POSITIVE 319 70 23 188 130 aact:AA00675|5end_glutaredoxin_2_462524..462754_POSITIVE 317 45 1 184 133 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 317 47 4 220 184 aact:AA01025|5end_hypothetical_protein_717522..717752_POSITIVE 312 77 31 156 112 aact:AA00715|5end_[2Fe-2S]_ferredoxin_490632..490862_POSITIVE 311 68 21 224 166 aact:AA01815|5end_probable_sodium-dependent_transmembrane_transport_protein_1222541..1222771_POSITIVE 310 45 3 77 24 aact:AA02561|5end_conserved_hypothetical_protein_1783553..1783783_POSITIVE 305 79 31 163 105 aact:AA00316|5end_conserved_hypothetical_protein_214445..214675_POSITIVE 305 56 19 65 25 aact:AA00269|5end_3-oxoacyl-(acyl-carrier-protein)_reductase/short-chain_alcohol_dehydrogenase_188608..188838_POSITIVE 303 77 33 156 104 aact:AA02926|5end_hypothetical_protein_2048136..2048366_POSITIVE 301 69 21 192 134 aact:AA02634|5end_rhamnosyltransferase;_glycosyltransferase_homolog_2_1832816..1833046_POSITIVE 301 70 28 198 165 aact:AA00119|5end_ATP-dependent_Clp_protease_proteolytic_subunit_(endopeptidase_Clp)_88387..88617_POSITIVE 301 70 28 57 24 aact:AA00117|5end_hypothetical_protein_88246..88476_POSITIVE 298 50 3 222 177 aact:AA02429|5end_methionine_aminopeptidase_1674320..1674550_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 357 44 3 98 47 aact:AA02075|3end_conserved_hypothetical_protein_1409979..1410129_POSITIVE 351 78 33 120 76 aact:AA00283|3end_glutamate_5-kinase_(gamma-glutamyl_kinase)_200015..200165_POSITIVE 333 79 40 54 5 aact:AA00314|3end_conserved_hypothetical_protein_(possible_Kmt1_protein)_214326..214476_POSITIVE 328 70 22 136 82 aact:AA01054|3end_conserved_hypothetical_protein_733940..734090_POSITIVE 327 77 31 132 91 aact:AA00342|3end_hypothetical_protein_229939..230089_POSITIVE 322 78 43 82 45 aact:AA02472|3end_conserved_hypothetical_protein_1708409..1708559_POSITIVE 319 44 9 45 9 aact:AA01805|3end_electron_transport_complex_protein_1216773..1216923_POSITIVE 312 77 31 141 97 aact:AA00713|3end_chaperone_protein_hscA,_heat_shock_cognate_66_490697..490847_POSITIVE 310 45 3 74 21 aact:AA02560|3end_integral_membrane_protein_1783630..1783780_POSITIVE 303 77 33 91 39 aact:AA02925|3end_conserved_hypothetical_protein_2048151..2048301_POSITIVE 301 70 28 149 116 aact:AA00117|3end_hypothetical_protein_88418..88568_POSITIVE 298 50 3 48 3 aact:AA02426|3end_uridylyltransferase_1674226..1674376_POSITIVE 297 77 32 53 2 aact:AA00288|3end_conserved_hypothetical_protein_201347..201497_POSITIVE 296 69 21 135 84 aact:AA02970|3end_tyrosine_phenol-lyase_2077391..2077541_POSITIVE 296 70 21 110 62 aact:AA02569|3end_hypothetical_protein_1789184..1789334_POSITIVE 296 68 21 111 68 aact:AA00691|3end_conserved_hypothetical_protein_(possible_multidrug/sugar_efflux_transporter)_473155..473305_POSITIVE 291 46 5 44 1 aact:AA00740|3end_citrate_lyase_related_biosynthesis_protein_(2-(5'-triphosphoribosyl)-3'-dephosphocoenzyme-A_synthase)_506749..506899_POSITIVE 290 73 31 112 61 aact:AA00585|3end_glycerol-3-phosphate_regulon_repressor_399778..399928_POSITIVE 289 70 28 112 73 aact:AA01467|3end_mannose-specific_phosphotransferase_system_IID_987214..987364_POSITIVE 288 45 4 59 12 aact:AA02688|3end_hydrogenase_4_component_E_1874692..1874842_POSITIVE