Origin IGS: tgatctagtctgccttcatgggtttacctgttggcgtgagatgatagttcacaatgacagtttgccttatgctatacagagcatgaactaatacagaaattgaatgcggtaatagagcgttttttcagcatgttaacatcagatgttttattcccgacaatttaaaggta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tacctttaaattgtcgggaataaaacatctgatgttaacatgctgaaaaaacgctctattaccgcattcaatttctgtattagttcatgctctgtatagcataaggcaaactgtcattgtgaactatcatctcacgccaacaggtaaacccatgaaggcagactagatca Mask Tandem Repeat Region ================================================ tgatctagtctgccttcatgggtttacctgttggcgtgagatgatagttcacaatgacagtttgccttatgctatacagagcatgaactaatacagaaattgaatgcggtaatagagcgttttttcagcatgttaacatcagatgttttattcccgacaatttaaaggta Find is-nt database================================================ Query_seq: AA01816:AA01817|AA01816:AA01817:hypothetical protein:hypothetical protein:->->:1224195..1224364 170 tgatctagtctgccttcatgggtttacctgttggcgtgagatgatagttcacaatgacagtttgccttatgctatacagagcatgaactaatacagaaattgaatgcggtaatagagcgttttttcagcatgttaacatcagatgttttattcccgacaatttaaaggta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01816:AA01817|AA01816:AA01817:hypothetical protein:hypothetical protein:->->:1224195..1224364 170 tgatctagtctgccttcatgggtttacctgttggcgtgagatgatagttcacaatgacagtttgccttatgctatacagagcatgaactaatacagaaattgaatgcggtaatagagcgttttttcagcatgttaacatcagatgttttattcccgacaatttaaaggta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01816:AA01817|AA01816:AA01817:hypothetical protein:hypothetical protein:->->:1224195..1224364 170 tgatctagtctgccttcatgggtttacctgttggcgtgagatgatagttcacaatgacagtttgccttatgctatacagagcatgaactaatacagaaattgaatgcggtaatagagcgttttttcagcatgttaacatcagatgttttattcccgacaatttaaaggta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgatctagtctgccttcatgggtttacctgttggcgtgagatgatagttcacaatgacagtttgccttatgctatacagagcatgaactaatacagaaattgaatgcggtaatagagcgttttttcagcatgttaacatcagatgttttattcccgacaatttaaaggta Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: - Start: 8 End: 100 ....... D A K M P K G T P T L H Y N V I V T Q R I S Y L A H V L V S M ...................................................................... tgatctagtctgccttcatgggtttacctgttggcgtgagatgatagttcacaatgacagtttgccttatgctatacagagcatgaactaatacagaaattgaatgcggtaatagagcgttttttcagcatgttaacatcagatgttttattcccgacaatttaaaggta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgatctagtctgccttcatgggtttacctgttggcgtgagatgatagttcacaatgacagtttgccttatgctatacagagcatgaactaatacagaaattgaatgcggtaatagagcgttttttcagcatgttaacatcagatgttttattcccgacaatttaaaggta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01816:AA01817|AA01816:AA01817:hypothetical protein:hypothetical protein:->->:1224195..1224364 170 tgatctagtctgccttcatgggtttacctgttggcgtgagatgatagttcacaatgacagtttgccttatgctatacagagcatgaactaatacagaaattgaatgcggtaatagagcgttttttcagcatgttaacatcagatgttttattcccgacaatttaaaggta Intra-Species Hit: Count: 1 Min: 1 Max: 170 Len: 170 Subject: aact_AA01816_AA01817|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1224195,1224364]|170 HSP 1 e-value: 7.0E-92 bit: 337.0 Len: 170 Query Start:1 Query End:170 Subject Strand: POSITIVE Subject Start: 1 Subject End: 170 tgatctagtctgccttcatgggtttacctgttggcgtgagatgatagttcacaatgacagtttgccttatgctatacagagcatgaactaatacagaaattgaatgcggtaatagagcgttttttcagcatgttaacatcagatgttttattcccgacaatttaaaggta tgatctagtctgccttcatgggtttacctgttggcgtgagatgatagttcacaatgacagtttgccttatgctatacagagcatgaactaatacagaaattgaatgcggtaatagagcgttttttcagcatgttaacatcagatgttttattcccgacaatttaaaggta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUCUAGUCUGCCUUCAUGGGUUUACCUGUUGGCGUGAGAUGAUAGUUCACAAUGACAGUUUGCCUUAUGCUAUACAGAGCAUGAACUAAUACAGAAAUUGAAUGCGGUAAUAGAGCGUUUUUUCAGCAUGUUAACAUCAGAUGUUUUAUUCCCGACAAUUUAAAGGUA ..........((((((....((((((((((((((((((((..(((.(((......))).)))...))))))))).)))).)..)))))).......((((((....(((.((((((((((((.....(.(((....)))))))))))))))).))).)))))).)))))) (-38.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UACCUUUAAAUUGUCGGGAAUAAAACAUCUGAUGUUAACAUGCUGAAAAAACGCUCUAUUACCGCAUUCAAUUUCUGUAUUAGUUCAUGCUCUGUAUAGCAUAAGGCAAACUGUCAUUGUGAACUAUCAUCUCACGCCAACAGGUAAACCCAUGAAGGCAGACUAGAUCA ............(((...............((((.....((((.((((....((.........))......)))).))))(((((((((((......)))....(((.....)))...)))))))).))))....(((..((((.....)).))..))).)))....... (-26.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 405 149 103 166 119 aact:AA00146|5end_UDP-glucose/GDP-mannose_dehydrogenase_105256..105486_POSITIVE 361 50 10 206 168 aact:AA01191|5end_AMP-binding_enzyme_816000..816230_POSITIVE 348 36 6 228 194 aact:AA02745|5end_isopentenyl_monophosphate_kinase_1923279..1923509_POSITIVE 320 46 3 223 185 aact:AA01759|5end_hydrogenase_isoenzymes_formation_protein_1188675..1188905_POSITIVE 317 69 21 70 20 aact:AA01972|5end_phosphotransacetylase_1332219..1332449_POSITIVE 316 50 4 151 103 aact:AA02827|5end_conserved_hypothetical_protein_1987021..1987251_POSITIVE 310 60 12 97 41 aact:AA01111|5end_conserved_hypothetical_protein_764765..764995_POSITIVE 304 45 1 98 49 aact:AA02260|5end_pseudouridylate_synthase_I_1537067..1537297_POSITIVE 300 60 11 72 13 aact:AA02365|5end_hflC_protein_1621997..1622227_POSITIVE 295 69 27 63 1 aact:AA01994|5end_chorismate_mutase;_prephenate_dehydrogenase_1354959..1355189_POSITIVE 294 38 1 197 160 aact:AA01998|5end_16S_rRNA_pseudouridine_synthase_1356842..1357072_POSITIVE 290 50 4 41 1 aact:AA01796|5end_branched-chain_amino_acid_aminotransferase_1211072..1211302_POSITIVE 290 43 5 51 8 aact:AA00093|5end_L-serine_dehydratase_(L-serine_deaminase)_69732..69962_POSITIVE 289 35 5 34 4 aact:AA01577|5end_conserved_hypothetical_transmembrane_protein_1063084..1063314_POSITIVE 288 47 8 66 30 aact:AA01183|5end_conserved_hypothetical_protein_810442..810672_POSITIVE 286 39 5 42 5 aact:AA00281|5end_phosphatidylserine_decarboxylase_proenzyme_197939..198169_POSITIVE 283 45 1 166 115 aact:AA00595|5end_hypothetical_protein_407194..407424_POSITIVE 281 58 15 140 87 aact:AA02579|5end_aminotransferase_nifS_protein_1795773..1796003_POSITIVE 281 59 12 231 176 aact:AA01113|5end_transcriptional_regulator_(merR_family)_764900..765130_POSITIVE 280 69 21 224 156 aact:AA01995|5end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355120..1355350_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 322 46 2 151 108 aact:AA00196|3end_conserved_hypothetical_protein_138015..138165_POSITIVE 304 45 1 79 30 aact:AA02259|3end_possible_RelE-like_protein_1537128..1537278_POSITIVE 300 60 11 69 10 aact:AA02363|3end_hflK_protein_1622074..1622224_POSITIVE 294 38 1 139 102 aact:AA01997|3end_probable_translation_factor_1356864..1357014_POSITIVE 288 47 8 54 18 aact:AA01182|3end_3-oxoacyl-(acyl-carrier-protein)_synthase_810510..810660_POSITIVE 281 58 15 131 78 aact:AA02577|3end_nuclease_1795844..1795994_POSITIVE 280 66 30 57 22 aact:AA00379|3end_possible_membrane_protein_256488..256638_POSITIVE 279 50 3 65 5 aact:AA00813|3end_hypothetical_protein_559021..559171_POSITIVE 276 51 11 87 54 aact:AA00633|3end_hypothetical_protein_435167..435317_POSITIVE 275 39 4 151 110 aact:AA02826|3end_conserved_hypothetical_protein_1987108..1987258_POSITIVE 273 158 116 137 100 aact:AA00399|3end_conserved_hypothetical_protein_268363..268513_POSITIVE 268 66 21 125 93 aact:AA02905|3end_conserved_hypothetical_protein_2033405..2033555_POSITIVE 266 39 1 117 85 aact:AA00724|3end_hypothetical_protein_498036..498186_POSITIVE 265 38 4 130 94 aact:AA00713|3end_chaperone_protein_hscA,_heat_shock_cognate_66_490697..490847_POSITIVE 264 50 2 105 49 aact:AA02293|3end_hypothetical_protein_1563709..1563859_POSITIVE 259 36 10 127 98 aact:AA02077|3end_probable_outer_membrane_protein_1412708..1412858_POSITIVE 259 146 102 151 99 aact:AA01384|3end_hypothetical_protein_935540..935690_POSITIVE 259 128 81 52 3 aact:AA00207|3end_D-ribose_ABC_transporter,_ATP-binding_protein_/_galactoside_ABC_transporter,_ATP-binding_protein_144177..144327_POSITIVE 258 57 11 54 1 aact:AA02022|3end_hypothetical_protein_1376754..1376904_POSITIVE 258 57 11 146 89 aact:AA00735|3end_citrate_lyase,_beta_chain_503626..503776_POSITIVE