Origin IGS: tagtttaaaagaaataatgctcagataatttatcctcatataatttgtctgagcgactttgaattcttaggaaaattctaaccaagctcagataagcaaattagcttggtgtgtattttttgcacaagcaaaaaagtaaaaggaagttt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaacttccttttacttttttgcttgtgcaaaaaatacacaccaagctaatttgcttatctgagcttggttagaattttcctaagaattcaaagtcgctcagacaaattatatgaggataaattatctgagcattatttcttttaaacta Mask Tandem Repeat Region ================================================ tagtttaaaagaaataatgctcagataatttatcctcatataatttgtctgagcgactttgaattcttaggaaaattctaaccaagctcagataagcaaattagcttggtgtgtattttttgcacaagcaaaaaagtaaaaggaagttt Find is-nt database================================================ Query_seq: AA01813:AA01814|AA01813:AA01814:electron transport complex protein:endonuclease III:->->:1221872..1222020 149 tagtttaaaagaaataatgctcagataatttatcctcatataatttgtctgagcgactttgaattcttaggaaaattctaaccaagctcagataagcaaattagcttggtgtgtattttttgcacaagcaaaaaagtaaaaggaagttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01813:AA01814|AA01813:AA01814:electron transport complex protein:endonuclease III:->->:1221872..1222020 149 tagtttaaaagaaataatgctcagataatttatcctcatataatttgtctgagcgactttgaattcttaggaaaattctaaccaagctcagataagcaaattagcttggtgtgtattttttgcacaagcaaaaaagtaaaaggaagttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01813:AA01814|AA01813:AA01814:electron transport complex protein:endonuclease III:->->:1221872..1222020 149 tagtttaaaagaaataatgctcagataatttatcctcatataatttgtctgagcgactttgaattcttaggaaaattctaaccaagctcagataagcaaattagcttggtgtgtattttttgcacaagcaaaaaagtaaaaggaagttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagtttaaaagaaataatgctcagataatttatcctcatataatttgtctgagcgactttgaattcttaggaaaattctaaccaagctcagataagcaaattagcttggtgtgtattttttgcacaagcaaaaaagtaaaaggaagttt Predict ORF larger than 30AA ================================================ tagtttaaaagaaataatgctcagataatttatcctcatataatttgtctgagcgactttgaattcttaggaaaattctaaccaagctcagataagcaaattagcttggtgtgtattttttgcacaagcaaaaaagtaaaaggaagttt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagtttaaaagaaataatgctcagataatttatcctcatataatttgtctgagcgactttgaattcttaggaaaattctaaccaagctcagataagcaaattagcttggtgtgtattttttgcacaagcaaaaaagtaaaaggaagttt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 58 HP_score: -2.8 Tail_Score: -4.43397 Start: 18 End: 55 Full_Region: ctaagaattcaaagt cgctcagacaaattat atgagg ataaattatctgagca ttatttcttttaaac .................cgctcagacaaattatatgaggataaattatctgagca.............................................................................................. TransTerm Strand: - Conf: 61 HP_score: -6.3 Tail_Score: -3.24954 Start: 19 End: 54 Full_Region: taagaattcaaagtc gctcagacaaattat atgagg ataaattatctgagc attatttcttttaaa ..................gctcagacaaattatatgaggataaattatctgagc............................................................................................... Find igs database================================================ Query_seq: AA01813:AA01814|AA01813:AA01814:electron transport complex protein:endonuclease III:->->:1221872..1222020 149 tagtttaaaagaaataatgctcagataatttatcctcatataatttgtctgagcgactttgaattcttaggaaaattctaaccaagctcagataagcaaattagcttggtgtgtattttttgcacaagcaaaaaagtaaaaggaagttt Intra-Species Hit: Count: 1 Min: 1 Max: 149 Len: 149 Subject: aact_AA01813_AA01814|electron transport complex protein:endonuclease III|POSITIVE:POSITIVE|[1221872,1222020]|149 HSP 1 e-value: 2.0E-79 bit: 295.0 Len: 149 Query Start:1 Query End:149 Subject Strand: POSITIVE Subject Start: 1 Subject End: 149 tagtttaaaagaaataatgctcagataatttatcctcatataatttgtctgagcgactttgaattcttaggaaaattctaaccaagctcagataagcaaattagcttggtgtgtattttttgcacaagcaaaaaagtaaaaggaagttt tagtttaaaagaaataatgctcagataatttatcctcatataatttgtctgagcgactttgaattcttaggaaaattctaaccaagctcagataagcaaattagcttggtgtgtattttttgcacaagcaaaaaagtaaaaggaagttt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUUUAAAAGAAAUAAUGCUCAGAUAAUUUAUCCUCAUAUAAUUUGUCUGAGCGACUUUGAAUUCUUAGGAAAAUUCUAACCAAGCUCAGAUAAGCAAAUUAGCUUGGUGUGUAUUUUUUGCACAAGCAAAAAAGUAAAAGGAAGUUU .................(((((((((((.((((......)))).)))))))))))........(((((............((((((((..(((......))))))))))).....((((((((....)))))))).....))))).... (-35.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAACUUCCUUUUACUUUUUUGCUUGUGCAAAAAAUACACACCAAGCUAAUUUGCUUAUCUGAGCUUGGUUAGAAUUUUCCUAAGAAUUCAAAGUCGCUCAGACAAAUUAUAUGAGGAUAAAUUAUCUGAGCAUUAUUUCUUUUAAACUA ..............((((((((....)))))))).....((((((((..............))))))))..((((((......))))))......(((((((.((.((((......)))).)).))))))).................. (-32.54) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 425 59 13 216 168 aact:AA00239|5end_hypothetical_protein_171213..171443_POSITIVE 326 130 81 65 6 aact:AA00237|5end_hypothetical_protein_170934..171164_POSITIVE 259 126 81 90 41 aact:AA01765|5end_Mu-like_prophage_FluMu_transposase_A_1191756..1191986_POSITIVE 257 89 44 202 166 aact:AA01848|5end_argininosuccinate_synthetase_1242278..1242508_POSITIVE 253 129 86 204 156 aact:AA01156|5end_chaperonin_heat_shock_protein_33_homolog_791459..791689_POSITIVE 251 130 82 114 52 aact:AA01207|5end_hypothetical_protein_826288..826518_POSITIVE 249 129 81 77 28 aact:AA02961|5end_pyrroline-5-carboxylate_reductase_2070785..2071015_POSITIVE 243 130 84 206 145 aact:AA00863|5end_conserved_hypothetical_protein_596679..596909_POSITIVE 238 130 81 210 156 aact:AA00575|5end_shikimate_5-dehydrogenase_393216..393446_POSITIVE 231 83 44 208 165 aact:AA00619|5end_hypothetical_protein_424131..424361_POSITIVE 226 129 81 84 39 aact:AA01187|5end_conserved_hypothetical_protein_813331..813561_POSITIVE 221 126 86 169 131 aact:AA01732|5end_multidrug_resistance_protein_A_1170677..1170907_POSITIVE 217 55 15 226 182 aact:AA00167|5end_possible_amino-acid_transporter_(sodium-alanine_symporters)_118239..118469_POSITIVE 213 124 82 51 12 aact:AA02409|5end_DNA_ligase_1653315..1653545_POSITIVE 213 99 51 136 73 aact:AA00570|5end_hypothetical_protein_391613..391843_POSITIVE 211 130 84 73 27 aact:AA01220|5end_competence_protein_F;_amidophosphoribosyltransferase_832775..833005_POSITIVE 210 111 81 198 161 aact:AA02996|5end_probable_cation_transport_protein_2093369..2093599_POSITIVE 210 130 86 71 22 aact:AA01360|5end_XAA-pro_aminopeptidase_(X-pro_aminopeptidase)_921047..921277_POSITIVE 206 98 53 221 168 aact:AA02355|5end_nicotinamide_phosphoribosyl_transferase_1612924..1613154_POSITIVE 204 102 64 104 58 aact:AA00658|5end_hypothetical_protein_451618..451848_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 425 59 13 92 44 aact:AA00237|3end_hypothetical_protein_171169..171319_POSITIVE 326 130 81 72 13 aact:AA00236|3end_anaerobic_ribonucleoside-triphosphate_reductase_171021..171171_POSITIVE 253 129 86 76 28 aact:AA01155|3end_hypothetical_protein_791411..791561_POSITIVE 251 130 82 73 11 aact:AA01206|3end_phosphoribosylformylglycinamidine_cyclo-ligase_826327..826477_POSITIVE 236 129 95 137 103 aact:AA00047|3end_formate_dependent_nitrite_reductase,_cytochrome_c552_38353..38503_POSITIVE 233 123 81 148 105 aact:AA01116|3end_hypothetical_protein_767840..767990_POSITIVE 226 129 81 74 29 aact:AA01185|3end_conserved_hypothetical_protein_(possible_membrane_protein)_813401..813551_POSITIVE 221 126 86 87 49 aact:AA01731|3end_multidrug_resistance_protein_B_1170675..1170825_POSITIVE 219 129 82 129 84 aact:AA02562|3end_adenine_specific_methylase_1785146..1785296_POSITIVE 211 130 84 73 27 aact:AA01217|3end_hypothetical_protein_832855..833005_POSITIVE 210 111 81 65 28 aact:AA02994|3end_probable_cation_transport_protein_2093316..2093466_POSITIVE 210 130 86 53 4 aact:AA01359|3end_conserved_hypothetical_protein_921109..921259_POSITIVE 204 54 18 72 35 aact:AA01813|3end_electron_transport_complex_protein_1221811..1221961_POSITIVE 203 126 81 65 22 aact:AA02128|3end_DNA_topoisomerase_I_1454078..1454228_POSITIVE 201 129 83 109 59 aact:AA00795|3end_iron(III)_dicitrate-binding_periplasmic_protein_precursor_545763..545913_POSITIVE 200 113 82 39 10 aact:AA02436|3end_periplasmic_protein_1681095..1681245_POSITIVE 200 138 106 141 103 aact:AA00895|3end_hypothetical_protein_621469..621619_POSITIVE 199 137 97 131 97 aact:AA02486|3end_topoisomerase_IV,_subunit_B_1726312..1726462_POSITIVE 199 129 82 39 6 aact:AA00983|3end_aldo-keto_reductase_688492..688642_POSITIVE 198 124 83 138 99 aact:AA02024|3end_acyl_carrier_protein_phosphodiesterase_1378739..1378889_POSITIVE