Origin IGS: taatttattccttcctgcccaaaggctttcggcgtgtaaaattcaccaaaatttttgaccgcacttttctaaaacacgcttttcagtgtataaaaaagtgcgatcgtttttgctagaatttatcccaattttttattgttcttaagcccgtaaatagcgataatacaccgcttacacgaacgaatcgaactct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agagttcgattcgttcgtgtaagcggtgtattatcgctatttacgggcttaagaacaataaaaaattgggataaattctagcaaaaacgatcgcacttttttatacactgaaaagcgtgttttagaaaagtgcggtcaaaaattttggtgaattttacacgccgaaagcctttgggcaggaaggaataaatta Mask Tandem Repeat Region ================================================ taatttattccttcctgcccaaaggctttcggcgtgtaaaattcaccaaaatttttgaccgcacttttctaaaacacgcttttcagtgtataaaaaagtgcgatcgtttttgctagaatttatcccaattttttattgttcttaagcccgtaaatagcgataatacaccgcttacacgaacgaatcgaactct Find is-nt database================================================ Query_seq: AA01804:AA01805|AA01804:AA01805:sugar nucleotide epimerase; cell division inhibitor:electron transport complex protein:->->:1216062..1216254 193 taatttattccttcctgcccaaaggctttcggcgtgtaaaattcaccaaaatttttgaccgcacttttctaaaacacgcttttcagtgtataaaaaagtgcgatcgtttttgctagaatttatcccaattttttattgttcttaagcccgtaaatagcgataatacaccgcttacacgaacgaatcgaactct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01804:AA01805|AA01804:AA01805:sugar nucleotide epimerase; cell division inhibitor:electron transport complex protein:->->:1216062..1216254 193 taatttattccttcctgcccaaaggctttcggcgtgtaaaattcaccaaaatttttgaccgcacttttctaaaacacgcttttcagtgtataaaaaagtgcgatcgtttttgctagaatttatcccaattttttattgttcttaagcccgtaaatagcgataatacaccgcttacacgaacgaatcgaactct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01804:AA01805|AA01804:AA01805:sugar nucleotide epimerase; cell division inhibitor:electron transport complex protein:->->:1216062..1216254 193 taatttattccttcctgcccaaaggctttcggcgtgtaaaattcaccaaaatttttgaccgcacttttctaaaacacgcttttcagtgtataaaaaagtgcgatcgtttttgctagaatttatcccaattttttattgttcttaagcccgtaaatagcgataatacaccgcttacacgaacgaatcgaactct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatttattccttcctgcccaaaggctttcggcgtgtaaaattcaccaaaatttttgaccgcacttttctaaaacacgcttttcagtgtataaaaaagtgcgatcgtttttgctagaatttatcccaattttttattgttcttaagcccgtaaatagcgataatacaccgcttacacgaacgaatcgaactct Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 3 End: 113 .. M Y S F L P K G F R R V K F T K I F D R T F L K H A F Q C I K K C D R F C ................................................................................ taatttattccttcctgcccaaaggctttcggcgtgtaaaattcaccaaaatttttgaccgcacttttctaaaacacgcttttcagtgtataaaaaagtgcgatcgtttttgctagaatttatcccaattttttattgttcttaagcccgtaaatagcgataatacaccgcttacacgaacgaatcgaactct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatttattccttcctgcccaaaggctttcggcgtgtaaaattcaccaaaatttttgaccgcacttttctaaaacacgcttttcagtgtataaaaaagtgcgatcgtttttgctagaatttatcccaattttttattgttcttaagcccgtaaatagcgataatacaccgcttacacgaacgaatcgaactct Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 47 HP_score: -2.6 Tail_Score: -3.44158 Start: 57 End: 105 Full_Region: TTCACCAAAATTTTT GACCGCACTTTTCTAAAACAC GCTTTTCA GTGTATAAAAAAGTGCGATC GTTTTTGCTAGAATT ........................................................GACCGCACTTTTCTAAAACACGCTTTTCAGTGTATAAAAAAGTGCGATC........................................................................................ TransTerm Strand: + Conf: 41 HP_score: -2.9 Tail_Score: -2.56601 Start: 60 End: 102 Full_Region: ACCAAAATTTTTGAC CGCACTTTTCTAAAACAC GCTTTTCA GTGTATAAAAAAGTGCG ATCGTTTTTGCTAGA ...........................................................CGCACTTTTCTAAAACACGCTTTTCAGTGTATAAAAAAGTGCG........................................................................................... Find igs database================================================ Query_seq: AA01804:AA01805|AA01804:AA01805:sugar nucleotide epimerase; cell division inhibitor:electron transport complex protein:->->:1216062..1216254 193 taatttattccttcctgcccaaaggctttcggcgtgtaaaattcaccaaaatttttgaccgcacttttctaaaacacgcttttcagtgtataaaaaagtgcgatcgtttttgctagaatttatcccaattttttattgttcttaagcccgtaaatagcgataatacaccgcttacacgaacgaatcgaactct Intra-Species Hit: Count: 1 Min: 1 Max: 193 Len: 193 Subject: aact_AA01804_AA01805|sugar nucleotide epimerase; cell division inhibitor:electron transport complex protein|POSITIVE:POSITIVE|[1216062,1216254]|193 HSP 1 e-value: 1.0E-105 bit: 383.0 Len: 193 Query Start:1 Query End:193 Subject Strand: POSITIVE Subject Start: 1 Subject End: 193 taatttattccttcctgcccaaaggctttcggcgtgtaaaattcaccaaaatttttgaccgcacttttctaaaacacgcttttcagtgtataaaaaagtgcgatcgtttttgctagaatttatcccaattttttattgttcttaagcccgtaaatagcgataatacaccgcttacacgaacgaatcgaactct taatttattccttcctgcccaaaggctttcggcgtgtaaaattcaccaaaatttttgaccgcacttttctaaaacacgcttttcagtgtataaaaaagtgcgatcgtttttgctagaatttatcccaattttttattgttcttaagcccgtaaatagcgataatacaccgcttacacgaacgaatcgaactct Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUUAUUCCUUCCUGCCCAAAGGCUUUCGGCGUGUAAAAUUCACCAAAAUUUUUGACCGCACUUUUCUAAAACACGCUUUUCAGUGUAUAAAAAAGUGCGAUCGUUUUUGCUAGAAUUUAUCCCAAUUUUUUAUUGUUCUUAAGCCCGUAAAUAGCGAUAAUACACCGCUUACACGAACGAAUCGAACUCU ...((.((((.(((..(((....)))....(((.....((((((..(((((....(((.(((((((((.((..((((........)))).)).))))))))).))).)))))...))))))....((((.....)))).......))).(((...((((.........)))))))..))).)))).))..... (-37.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAGUUCGAUUCGUUCGUGUAAGCGGUGUAUUAUCGCUAUUUACGGGCUUAAGAACAAUAAAAAAUUGGGAUAAAUUCUAGCAAAAACGAUCGCACUUUUUUAUACACUGAAAAGCGUGUUUUAGAAAAGUGCGGUCAAAAAUUUUGGUGAAUUUUACACGCCGAAAGCCUUUGGGCAGGAAGGAAUAAAUUA ...((((.....(((((((..(((((((...)))))))...)))))))....))))..................(((((..(......(((((((((((((((.((((........))))..))))))))))))))).....((((((((.........))))))))(((....)))..)..)))))...... (-57.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 387 70 24 173 124 aact:AA01374|5end_tRNA/rRNA_methyltransferase_930283..930513_POSITIVE 375 70 22 185 137 aact:AA01251|5end_hypothetical_protein_851825..852055_POSITIVE 370 100 56 156 120 aact:AA02082|5end_glutaminyl-tRNA_synthetase_1416101..1416331_POSITIVE 369 69 24 124 68 aact:AA00988|5end_conserved_hypothetical_protein_690898..691128_POSITIVE 360 38 5 199 158 aact:AA00432|5end_conserved_hypothetical_protein;_possible_methyltransferase_293897..294127_POSITIVE 339 47 4 131 87 aact:AA01753|5end_conserved_hypothetical_protein_1185322..1185552_POSITIVE 330 68 24 227 171 aact:AA01810|5end_electron_transport_complex_protein_1219250..1219480_POSITIVE 324 67 23 215 179 aact:AA02479|5end_outer_membrane_protein_34_1715134..1715364_POSITIVE 323 88 49 168 113 aact:AA01988|5end_dihydrolipoamide_dehydrogenase;_lipoamide_dehydrogenase_1350517..1350747_POSITIVE 323 88 49 66 11 aact:AA01987|5end_hypothetical_protein_1350415..1350645_POSITIVE 320 70 32 209 170 aact:AA02754|5end_conserved_hypothetical_protein_1928949..1929179_POSITIVE 318 70 21 160 115 aact:AA02993|5end_6-phosphofructokinase_2091766..2091996_POSITIVE 315 80 35 176 129 aact:AA00239|5end_hypothetical_protein_171213..171443_POSITIVE 312 70 21 207 153 aact:AA01830|5end_2,3-cyclic-nucleotide_2-phosphodiesterase_1231556..1231786_POSITIVE 311 100 58 156 112 aact:AA01390|5end_LexA_repressor_937059..937289_POSITIVE 311 100 58 53 9 aact:AA01389|5end_hypothetical_protein_936956..937186_POSITIVE 303 70 22 54 1 aact:AA01829|5end_hypothetical_protein_1231403..1231633_POSITIVE 302 85 44 156 110 aact:AA02047|5end_galactitol-1-phosphate_5-dehydrogenase_1393633..1393863_POSITIVE 296 69 24 83 44 aact:AA00908|5end_probable_sigma-E_factor_regulatory_protein_626076..626306_POSITIVE 296 80 32 62 18 aact:AA00697|5end_hypothetical_protein_476999..477229_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 375 70 22 60 12 aact:AA01250|3end_nadAB_transcriptional_regulator_851780..851930_POSITIVE 357 67 26 106 63 aact:AA01021|3end_hypothetical_protein_714276..714426_POSITIVE 339 47 4 121 77 aact:AA01751|3end_conserved_hypothetical_protein_1185392..1185542_POSITIVE 323 88 49 71 16 aact:AA01986|3end_pyruvate_dehydrogenase_complex_E2_1350500..1350650_POSITIVE 320 70 32 48 9 aact:AA02751|3end_L-lactate_permease_1928868..1929018_POSITIVE 318 70 21 54 9 aact:AA02992|3end_RNA_methyltransferase_2091740..2091890_POSITIVE 315 80 35 52 5 aact:AA00237|3end_hypothetical_protein_171169..171319_POSITIVE 302 85 44 130 84 aact:AA02046|3end_PTS_system_galactitol-specific_enzyme_IIC_1393687..1393837_POSITIVE 296 84 53 99 62 aact:AA01351|3end_probable_sulfatase_916393..916543_POSITIVE 296 69 24 70 31 aact:AA00906|3end_sigma-E_factor_regulatory_protein_626143..626293_POSITIVE 296 80 32 89 45 aact:AA00696|3end_iron(III)_ABC_transporter,_periplasmic_iron-compound-binding_protein_477106..477256_POSITIVE 293 86 50 53 15 aact:AA01561|3end_conserved_hypothetical_protein_1046971..1047121_POSITIVE 292 84 52 109 67 aact:AA01966|3end_2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_pyrophosphokinase_1331514..1331664_POSITIVE 290 70 21 73 31 aact:AA00659|3end_heat_shock_protein_453027..453177_POSITIVE 289 68 21 79 37 aact:AA02781|3end_protein-export_membrane_protein_1945981..1946131_POSITIVE 288 68 21 90 36 aact:AA02564|3end_transketolase_N-terminal_section_1786783..1786933_POSITIVE 287 70 24 134 80 aact:AA02998|3end_conserved_hypothetical_protein_2094350..2094500_POSITIVE 286 68 29 67 27 aact:AA01439|3end_probable_ferredoxin_(fdx-2)_966498..966648_POSITIVE 286 67 22 89 39 aact:AA00938|3end_L-xylulokinase_648358..648508_POSITIVE 284 187 146 151 109 aact:AA01547|3end_deoxyribose-phosphate_aldolase_1037019..1037169_POSITIVE