Origin IGS: tagaaaatagttaccctaaaattttatttatccttatctattattctgggagttttataggcttcctgataaaaaatcccaatgagtaaataggtaacatgtgaataaattctgttcaaacagacccgtaaaacttgaactaagataattttttcataaaatgattttcactcttattttaccaacaataaattaaagtcttt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaagactttaatttattgttggtaaaataagagtgaaaatcattttatgaaaaaattatcttagttcaagttttacgggtctgtttgaacagaatttattcacatgttacctatttactcattgggattttttatcaggaagcctataaaactcccagaataatagataaggataaataaaattttagggtaactattttcta Mask Tandem Repeat Region ================================================ tagaaaatagttaccctaaaattttatttatccttatctattattctgggagttttataggcttcctgataaaaaatcccaatgagtaaataggtaacatgtgaataaattctgttcaaacagacccgtaaaacttgaactaagataattttttcataaaatgattttcactcttattttaccaacaataaattaaagtcttt Find is-nt database================================================ Query_seq: AA01798:AA01799|AA01798:AA01799:hypothetical protein:polysacharide biosynthesis protein (Orf14 of cluster):->->:1212396..1212598 203 tagaaaatagttaccctaaaattttatttatccttatctattattctgggagttttataggcttcctgataaaaaatcccaatgagtaaataggtaacatgtgaataaattctgttcaaacagacccgtaaaacttgaactaagataattttttcataaaatgattttcactcttattttaccaacaataaattaaagtcttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01798:AA01799|AA01798:AA01799:hypothetical protein:polysacharide biosynthesis protein (Orf14 of cluster):->->:1212396..1212598 203 tagaaaatagttaccctaaaattttatttatccttatctattattctgggagttttataggcttcctgataaaaaatcccaatgagtaaataggtaacatgtgaataaattctgttcaaacagacccgtaaaacttgaactaagataattttttcataaaatgattttcactcttattttaccaacaataaattaaagtcttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01798:AA01799|AA01798:AA01799:hypothetical protein:polysacharide biosynthesis protein (Orf14 of cluster):->->:1212396..1212598 203 tagaaaatagttaccctaaaattttatttatccttatctattattctgggagttttataggcttcctgataaaaaatcccaatgagtaaataggtaacatgtgaataaattctgttcaaacagacccgtaaaacttgaactaagataattttttcataaaatgattttcactcttattttaccaacaataaattaaagtcttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagaaaatagttaccctaaaattttatttatccttatctattattctgggagttttataggcttcctgataaaaaatcccaatgagtaaataggtaacatgtgaataaattctgttcaaacagacccgtaaaacttgaactaagataattttttcataaaatgattttcactcttattttaccaacaataaattaaagtcttt Predict ORF larger than 30AA ================================================ Protein_Len: 41 Strand: - Start: 27 End: 149 .......................... K D K D I I R P T K Y A E Q Y F I G I L L Y T V H S Y I R N L C V R L V Q V L I M ...................................................... tagaaaatagttaccctaaaattttatttatccttatctattattctgggagttttataggcttcctgataaaaaatcccaatgagtaaataggtaacatgtgaataaattctgttcaaacagacccgtaaaacttgaactaagataattttttcataaaatgattttcactcttattttaccaacaataaattaaagtcttt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagaaaatagttaccctaaaattttatttatccttatctattattctgggagttttataggcttcctgataaaaaatcccaatgagtaaataggtaacatgtgaataaattctgttcaaacagacccgtaaaacttgaactaagataattttttcataaaatgattttcactcttattttaccaacaataaattaaagtcttt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01798:AA01799|AA01798:AA01799:hypothetical protein:polysacharide biosynthesis protein (Orf14 of cluster):->->:1212396..1212598 203 tagaaaatagttaccctaaaattttatttatccttatctattattctgggagttttataggcttcctgataaaaaatcccaatgagtaaataggtaacatgtgaataaattctgttcaaacagacccgtaaaacttgaactaagataattttttcataaaatgattttcactcttattttaccaacaataaattaaagtcttt Intra-Species Hit: Count: 1 Min: 1 Max: 203 Len: 203 Subject: aact_AA01798_AA01799|hypothetical protein:polysacharide biosynthesis protein (Orf14 of cluster)|POSITIVE:POSITIVE|[1212396,1212598]|203 HSP 1 e-value: 1.0E-111 bit: 402.0 Len: 203 Query Start:1 Query End:203 Subject Strand: POSITIVE Subject Start: 1 Subject End: 203 tagaaaatagttaccctaaaattttatttatccttatctattattctgggagttttataggcttcctgataaaaaatcccaatgagtaaataggtaacatgtgaataaattctgttcaaacagacccgtaaaacttgaactaagataattttttcataaaatgattttcactcttattttaccaacaataaattaaagtcttt tagaaaatagttaccctaaaattttatttatccttatctattattctgggagttttataggcttcctgataaaaaatcccaatgagtaaataggtaacatgtgaataaattctgttcaaacagacccgtaaaacttgaactaagataattttttcataaaatgattttcactcttattttaccaacaataaattaaagtcttt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAAAAUAGUUACCCUAAAAUUUUAUUUAUCCUUAUCUAUUAUUCUGGGAGUUUUAUAGGCUUCCUGAUAAAAAAUCCCAAUGAGUAAAUAGGUAACAUGUGAAUAAAUUCUGUUCAAACAGACCCGUAAAACUUGAACUAAGAUAAUUUUUUCAUAAAAUGAUUUUCACUCUUAUUUUACCAACAAUAAAUUAAAGUCUUU .(((...(((((..........(((((((((..((((((((((((((((((.(((((((((...))).))))))..)))))..)))).)))))))))...))))))))).(((((....)))))...((((((...((...(((((.(((((.....))))).)))))...))...))))))........)))))...))).. (-35.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAGACUUUAAUUUAUUGUUGGUAAAAUAAGAGUGAAAAUCAUUUUAUGAAAAAAUUAUCUUAGUUCAAGUUUUACGGGUCUGUUUGAACAGAAUUUAUUCACAUGUUACCUAUUUACUCAUUGGGAUUUUUUAUCAGGAAGCCUAUAAAACUCCCAGAAUAAUAGAUAAGGAUAAAUAAAAUUUUAGGGUAACUAUUUUCUA .....(((((.((((((((((((((.....(((((((..((((...)))).............(((((((.............)))))))....))))))).....)))))...........(((((..((((((.(((...))))))))).))))).)))))))))))))).............(((((........))))) (-33.02) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 258 68 38 180 141 aact:AA01146|5end_conserved_hypothetical_protein_784433..784663_POSITIVE 255 157 111 214 168 aact:AA02094|5end_glycogen_phosphorylase;_glycogen_synthase_1426236..1426466_POSITIVE 252 108 67 121 78 aact:AA02834|5end_colicin_transport_protein_1991269..1991499_POSITIVE 252 127 83 118 74 aact:AA01273|5end_hypothetical_protein_864362..864592_POSITIVE 229 81 46 223 186 aact:AA02910|5end_ribonuclease_E_2036137..2036367_POSITIVE 229 130 84 216 171 aact:AA02733|5end_cell_division_protein_K;_sporulation_protein_spoIIIE_1912208..1912438_POSITIVE 224 68 35 105 58 aact:AA01724|5end_valyl-tRNA_synthetase_1164446..1164676_POSITIVE 221 97 60 110 74 aact:AA00314|5end_conserved_hypothetical_protein_(possible_Kmt1_protein)_212999..213229_POSITIVE 215 88 41 77 26 aact:AA01039|5end_transcriptional_regulator_(possible_murein_gene_regulator)_723864..724094_POSITIVE 213 68 30 150 113 aact:AA00658|5end_hypothetical_protein_451618..451848_POSITIVE 208 80 37 108 69 aact:AA02970|5end_tyrosine_phenol-lyase_2076373..2076603_POSITIVE 207 69 26 178 130 aact:AA00140|5end_hypothetical_protein_100646..100876_POSITIVE 203 68 38 145 114 aact:AA01456|5end_D-xylose_ABC_transporter,_ATP-binding_protein_976089..976319_POSITIVE 202 187 141 169 122 aact:AA01053|5end_conserved_hypothetical_protein_733119..733349_POSITIVE 201 84 45 116 73 aact:AA02976|5end_UDP-2,3-diacylglucosamine_hydrolase_2080229..2080459_POSITIVE 200 197 165 125 92 aact:AA01619|5end_phospho-2-dehydro-3-deoxyheptonate_aldolase_(DAHP_synthase)_1094422..1094652_POSITIVE 197 144 112 40 15 aact:AA01911|5end_hypothetical_protein_1287791..1288021_POSITIVE 196 97 60 199 168 aact:AA02838|5end_conserved_hypothetical_protein_1993016..1993246_POSITIVE 195 96 57 154 123 aact:AA02688|5end_hydrogenase_4_component_E_1873977..1874207_POSITIVE 195 88 47 123 79 aact:AA02473|5end_conserved_hypothetical_protein_1708571..1708801_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 259 89 41 50 2 aact:AA01798|3end_hypothetical_protein_1212335..1212485_POSITIVE 258 68 38 127 88 aact:AA01145|3end_glycyl-tRNA_synthetase_alpha_subunit_784460..784610_POSITIVE 255 157 111 88 42 aact:AA02092|3end_glycogen_synthase_1426190..1426340_POSITIVE 252 108 67 101 58 aact:AA02832|3end_outer_membrane_integrity_protein_1991329..1991479_POSITIVE 229 130 84 120 75 aact:AA02731|3end_outer_membrane_lipoprotein_carrier_precursor_1912192..1912342_POSITIVE 221 97 60 88 52 aact:AA00313|3end_hypothetical_protein_213057..213207_POSITIVE 208 80 37 72 33 aact:AA02969|3end_beta_tyrosinase_(tyrosine_phenol-lyase)_2076417..2076567_POSITIVE 208 68 43 126 91 aact:AA00135|3end_S-adenosylmethionine:_2-demethylmenaquinone_methyltransferase_(menaquinone_biosynthesis_protein)_96880..97030_POSITIVE 206 68 30 144 110 aact:AA00700|3end_iron(III)_ABC_transporter,_ATP-binding_protein_481458..481608_POSITIVE 204 70 46 95 64 aact:AA00649|3end_peptide_chain_release_factor_1_(RF-1)_444355..444505_POSITIVE 203 68 38 82 51 aact:AA01455|3end_D-xylose_ABC_transporter,_periplasmic-binding_protein_976106..976256_POSITIVE 202 187 141 142 95 aact:AA01051|3end_iron_(III)_ABC_transporter,_ATP-binding_protein_733172..733322_POSITIVE 201 84 45 120 77 aact:AA02975|3end_conserved_hypothetical_protein_2080313..2080463_POSITIVE 196 97 60 80 49 aact:AA02837|3end_conserved_hypothetical_protein_1992977..1993127_POSITIVE 195 96 57 140 109 aact:AA02687|3end_hydrogenase-4_component_D_1874043..1874193_POSITIVE 194 81 46 58 15 aact:AA00921|3end_conserved_hypothetical_protein_634014..634164_POSITIVE 191 97 57 61 19 aact:AA00795|3end_iron(III)_dicitrate-binding_periplasmic_protein_precursor_545763..545913_POSITIVE 189 124 83 119 70 aact:AA01936|3end_tyrosyl-tRNA_synthetase_1308307..1308457_POSITIVE 189 135 92 115 69 aact:AA01477|3end_type_I_restriction_enzyme_R_protein_994479..994629_POSITIVE 189 83 48 40 11 aact:AA01067|3end_30S_ribosomal_protein_S2_739697..739847_POSITIVE