Origin IGS: tagtctttttttccatgaagagcagctacgatgaattatcatttggagcacttttgccatacatggcataaaagtcgcattttgttgacgtagcgcatagtctctcaggtatattcctgttgtgacatattattttatgatttttgtctttttatttttagggtgattt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaatcaccctaaaaataaaaagacaaaaatcataaaataatatgtcacaacaggaatatacctgagagactatgcgctacgtcaacaaaatgcgacttttatgccatgtatggcaaaagtgctccaaatgataattcatcgtagctgctcttcatggaaaaaaagacta Mask Tandem Repeat Region ================================================ tagtctttttttccatgaagagcagctacgatgaattatcatttggagcacttttgccatacatggcataaaagtcgcattttgttgacgtagcgcatagtctctcaggtatattcctgttgtgacatattattttatgatttttgtctttttatttttagggtgattt Find is-nt database================================================ Query_seq: AA01782:AA01784|AA01782:AA01784:molybdenum transport protein; transcriptional regulator:sodium-dependent transporter:->->:1203796..1203964 169 tagtctttttttccatgaagagcagctacgatgaattatcatttggagcacttttgccatacatggcataaaagtcgcattttgttgacgtagcgcatagtctctcaggtatattcctgttgtgacatattattttatgatttttgtctttttatttttagggtgattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01782:AA01784|AA01782:AA01784:molybdenum transport protein; transcriptional regulator:sodium-dependent transporter:->->:1203796..1203964 169 tagtctttttttccatgaagagcagctacgatgaattatcatttggagcacttttgccatacatggcataaaagtcgcattttgttgacgtagcgcatagtctctcaggtatattcctgttgtgacatattattttatgatttttgtctttttatttttagggtgattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01782:AA01784|AA01782:AA01784:molybdenum transport protein; transcriptional regulator:sodium-dependent transporter:->->:1203796..1203964 169 tagtctttttttccatgaagagcagctacgatgaattatcatttggagcacttttgccatacatggcataaaagtcgcattttgttgacgtagcgcatagtctctcaggtatattcctgttgtgacatattattttatgatttttgtctttttatttttagggtgattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagtctttttttccatgaagagcagctacgatgaattatcatttggagcacttttgccatacatggcataaaagtcgcattttgttgacgtagcgcatagtctctcaggtatattcctgttgtgacatattattttatgatttttgtctttttatttttagggtgattt Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: - Start: 29 End: 133 ............................ S S N D N P A S K G Y M A Y F D C K T S T A C L R E P I N R N H C I M .................................... Protein_Len: 38 Strand: - Start: 1 End: 114 L R K K W S S C S R H I I M Q L V K A M C P M F T A N Q Q R L A Y D R L Y M ....................................................... tagtctttttttccatgaagagcagctacgatgaattatcatttggagcacttttgccatacatggcataaaagtcgcattttgttgacgtagcgcatagtctctcaggtatattcctgttgtgacatattattttatgatttttgtctttttatttttagggtgattt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagtctttttttccatgaagagcagctacgatgaattatcatttggagcacttttgccatacatggcataaaagtcgcattttgttgacgtagcgcatagtctctcaggtatattcctgttgtgacatattattttatgatttttgtctttttatttttagggtgattt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01782:AA01784|AA01782:AA01784:molybdenum transport protein; transcriptional regulator:sodium-dependent transporter:->->:1203796..1203964 169 tagtctttttttccatgaagagcagctacgatgaattatcatttggagcacttttgccatacatggcataaaagtcgcattttgttgacgtagcgcatagtctctcaggtatattcctgttgtgacatattattttatgatttttgtctttttatttttagggtgattt Intra-Species Hit: Count: 1 Min: 13 Max: 127 Len: 115 Subject: aact_AA01782_AA01784|molybdenum transport protein; transcriptional regulator:sodium-dependent transporter|POSITIVE:POSITIVE|[1203796,1203964]|169 HSP 1 e-value: 5.0E-59 bit: 228.0 Len: 115 Query Start:13 Query End:127 Subject Strand: POSITIVE Subject Start: 13 Subject End: 127 ............ccatgaagagcagctacgatgaattatcatttggagcacttttgccatacatggcataaaagtcgcattttgttgacgtagcgcatagtctctcaggtatattcctgttgtgaca.......................................... ............ccatgaagagcagctacgatgaattatcatttggagcacttttgccatacatggcataaaagtcgcattttgttgacgtagcgcatagtctctcaggtatattcctgttgtgaca.......................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CCAUGAAGAGCAGCUACGAUGAAUUAUCAUUUGGAGCACUUUUGCCAUACAUGGCAUAAAAGUCGCAUUUUGUUGACGUAGCGCAUAGUCUCUCAGGUAUAUUCCUGUUGUGACA .........((.((((((.(((....)))......((((((((((((....)))))..))))).))..........))))))))...(((.(.((((......))))..).))). (-32.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUCACAACAGGAAUAUACCUGAGAGACUAUGCGCUACGUCAACAAAAUGCGACUUUUAUGCCAUGUAUGGCAAAAGUGCUCCAAAUGAUAAUUCAUCGUAGCUGCUCUUCAUGG .(((....((((......))))...)))...((((((((..........((.(((((..(((((....)))))))))))).....((((....)))))))))).))......... (-28.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 329 110 64 185 128 aact:AA00506|5end_ATP-dependent_RNA_helicase_protein_351681..351911_POSITIVE 329 110 64 65 8 aact:AA00505|5end_hypothetical_protein_351561..351791_POSITIVE 327 89 41 210 151 aact:AA01996|5end_prephenate_dehydrogenase_1355314..1355544_POSITIVE 312 89 43 102 49 aact:AA00688|5end_curved_DNA-binding_protein_469165..469395_POSITIVE 310 67 21 148 100 aact:AA01609|5end_p-aminobenzoate_synthase_component_I_homolog_1087903..1088133_POSITIVE 300 79 31 91 48 aact:AA00581|5end_thiosulfate_sulfurtransferase_(rhodanese)_397605..397835_POSITIVE 298 107 63 216 167 aact:AA01840|5end_conserved_hypothetical_protein_1237088..1237318_POSITIVE 296 90 42 119 67 aact:AA01477|5end_type_I_restriction_enzyme_R_protein_992249..992479_POSITIVE 295 110 61 197 140 aact:AA00324|5end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_220147..220377_POSITIVE 286 92 51 230 190 aact:AA00778|5end_trp_operon_repressor_536568..536798_POSITIVE 278 69 24 44 1 aact:AA01611|5end_anthranilate_synthase_component_II_1088976..1089206_POSITIVE 277 46 1 85 39 aact:AA02963|5end_opacity_associated_protein_B_2072302..2072532_POSITIVE 275 105 64 206 161 aact:AA01014|5end_UTP-glucose-1-phosphate_uridylyltransferase_709469..709699_POSITIVE 274 88 45 74 29 aact:AA01482|5end_ATP_synthase_F1,_subunit_beta_996285..996515_POSITIVE 274 87 41 77 21 aact:AA01423|5end_cell_division_protein_X,_ABC_system_permease_956668..956898_POSITIVE 273 34 1 198 163 aact:AA02166|5end_phenylalanyl-tRNA_synthetase,_alpha_subunit_1482200..1482430_POSITIVE 272 80 37 209 168 aact:AA00416|5end_D-methionine_transport_system_permease_protein_(ABC_transport_system)_282677..282907_POSITIVE 271 110 63 163 110 aact:AA02875|5end_conserved_hypothetical_protein_(2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol_hydroxylase)_2017930..2018160_POSITIVE 270 90 41 124 65 aact:AA01781|5end_molybdenum_ABC_transporter,_periplasmic_substrate-binding_1201891..1202121_POSITIVE 269 90 41 222 160 aact:AA01510|5end_ABC_transporter,_ATP-binding_protein/permease_1016430..1016660_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 327 89 41 67 8 aact:AA01994|3end_chorismate_mutase;_prephenate_dehydrogenase_1355251..1355401_POSITIVE 312 89 43 76 23 aact:AA00686|3end_hypothetical_protein_469219..469369_POSITIVE 310 67 21 142 94 aact:AA01608|3end_conserved_hypothetical_protein_1087977..1088127_POSITIVE 297 83 41 142 98 aact:AA01750|3end_MTA/SAH_nucleosidase_[Includes:_5'-methylthioadenosine_nucleosidase;_S-adenosylhomocysteine_nucleosidase]_1184519..1184669_POSITIVE 293 99 52 145 81 aact:AA00505|3end_hypothetical_protein_351733..351883_POSITIVE 275 105 64 127 82 aact:AA01013|3end_phosphomannomutase_709470..709620_POSITIVE 274 87 41 65 9 aact:AA01422|3end_cell_division_protein_E,_ABC_system_ATP-binding_protein_956736..956886_POSITIVE 270 90 41 87 28 aact:AA01780|3end_ABC-related_molybdenum_transport_system_permease_protein_1201934..1202084_POSITIVE 270 90 44 51 1 aact:AA01476|3end_hypothetical_protein_992261..992411_POSITIVE 263 46 10 60 25 aact:AA01661|3end_molybdopterin_biosynthesis_protein_1126192..1126342_POSITIVE 263 48 2 140 86 aact:AA01027|3end_Na(+)-translocating_NADH-quinone_reductase_subunit_F_718988..719138_POSITIVE 262 48 1 68 19 aact:AA01130|3end_conserved_hypothetical_protein_775023..775173_POSITIVE 261 66 28 52 6 aact:AA01859|3end_hypothetical_protein_1251890..1252040_POSITIVE 260 76 31 73 27 aact:AA01358|3end_conserved_hypothetical_protein_920387..920537_POSITIVE 259 97 51 142 110 aact:AA02695|3end_hydrogenase-3_component_G_1879418..1879568_POSITIVE 258 90 42 141 85 aact:AA01547|3end_deoxyribose-phosphate_aldolase_1037019..1037169_POSITIVE 258 97 52 83 28 aact:AA01152|3end_sec-independent_protein_translocase_protein_790205..790355_POSITIVE 257 110 61 149 91 aact:AA00350|3end_50S_ribosomal_protein_L10_234189..234339_POSITIVE 257 94 51 144 95 aact:AA00211|3end_hypothetical_protein_146163..146313_POSITIVE 255 97 51 131 91 aact:AA03012|3end_conserved_hypothetical_protein_2104556..2104706_POSITIVE