Origin IGS: aatacaaactccgtttttggttagtaaataaattatatatcgcgcagattatacaacgatgagcgcaatataaaaatatttttgaacaagaaaagatcacaagattgcgtatgtgccaaacgatttatcggcgttcttatttagaacaaaatatagtatgatgtgcaaccgataaaccatgtcataaaacgatatattccacagcgaattttgaggggaaaaccac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gtggttttcccctcaaaattcgctgtggaatatatcgttttatgacatggtttatcggttgcacatcatactatattttgttctaaataagaacgccgataaatcgtttggcacatacgcaatcttgtgatcttttcttgttcaaaaatatttttatattgcgctcatcgttgtataatctgcgcgatatataatttatttactaaccaaaaacggagtttgtatt Mask Tandem Repeat Region ================================================ aatacaaactccgtttttggttagtaaataaattatatatcgcgcagattatacaacgatgagcgcaatataaaaatatttttgaacaagaaaagatcacaagattgcgtatgtgccaaacgatttatcggcgttcttatttagaacaaaatatagtatgatgtgcaaccgataaaccatgtcataaaacgatatattccacagcgaattttgaggggaaaaccac Find is-nt database================================================ Query_seq: AA01781:AA01782|AA01781:AA01782:molybdenum ABC transporter, periplasmic substrate-binding:molybdenum transport protein; transcriptional regulator:->->:1202793..1203018 226 aatacaaactccgtttttggttagtaaataaattatatatcgcgcagattatacaacgatgagcgcaatataaaaatatttttgaacaagaaaagatcacaagattgcgtatgtgccaaacgatttatcggcgttcttatttagaacaaaatatagtatgatgtgcaaccgataaaccatgtcataaaacgatatattccacagcgaattttgaggggaaaaccac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01781:AA01782|AA01781:AA01782:molybdenum ABC transporter, periplasmic substrate-binding:molybdenum transport protein; transcriptional regulator:->->:1202793..1203018 226 aatacaaactccgtttttggttagtaaataaattatatatcgcgcagattatacaacgatgagcgcaatataaaaatatttttgaacaagaaaagatcacaagattgcgtatgtgccaaacgatttatcggcgttcttatttagaacaaaatatagtatgatgtgcaaccgataaaccatgtcataaaacgatatattccacagcgaattttgaggggaaaaccac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01781:AA01782|AA01781:AA01782:molybdenum ABC transporter, periplasmic substrate-binding:molybdenum transport protein; transcriptional regulator:->->:1202793..1203018 226 aatacaaactccgtttttggttagtaaataaattatatatcgcgcagattatacaacgatgagcgcaatataaaaatatttttgaacaagaaaagatcacaagattgcgtatgtgccaaacgatttatcggcgttcttatttagaacaaaatatagtatgatgtgcaaccgataaaccatgtcataaaacgatatattccacagcgaattttgaggggaaaaccac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatacaaactccgtttttggttagtaaataaattatatatcgcgcagattatacaacgatgagcgcaatataaaaatatttttgaacaagaaaagatcacaagattgcgtatgtgccaaacgatttatcggcgttcttatttagaacaaaatatagtatgatgtgcaaccgataaaccatgtcataaaacgatatattccacagcgaattttgaggggaaaaccac Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 28 End: 141 ........................... M N Y I S R R L Y N D E R N I K I F L N K K R S Q D C V C A K R F I G V L I ..................................................................................... Protein_Len: 35 Strand: - Start: 100 End: 204 ................................................................................................... L I A Y T G F S K D A N K N L V F Y L I I H L R Y V M D Y F S I N W M ...................... Protein_Len: 35 Strand: - Start: 36 End: 140 ................................... I D R L N Y L S S R L I F I N K F L F L D C S Q T H A L R N I P T R M ...................................................................................... Protein_Len: 41 Strand: - Start: 2 End: 124 . Y L S R K Q N T F L N Y I A C I I C R H A C Y L F I K S C S F I V L N R I H W V M ...................................................................................................... aatacaaactccgtttttggttagtaaataaattatatatcgcgcagattatacaacgatgagcgcaatataaaaatatttttgaacaagaaaagatcacaagattgcgtatgtgccaaacgatttatcggcgttcttatttagaacaaaatatagtatgatgtgcaaccgataaaccatgtcataaaacgatatattccacagcgaattttgaggggaaaaccac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 84 Start: 48 End: 77 ...............................................ATTTTTATATTGCGCTCATCGTTGTATAAT..................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatacaaactccgtttttggttagtaaataaattatatatcgcgcagattatacaacgatgagcgcaatataaaaatatttttgaacaagaaaagatcacaagattgcgtatgtgccaaacgatttatcggcgttcttatttagaacaaaatatagtatgatgtgcaaccgataaaccatgtcataaaacgatatattccacagcgaattttgaggggaaaaccac Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01781:AA01782|AA01781:AA01782:molybdenum ABC transporter, periplasmic substrate-binding:molybdenum transport protein; transcriptional regulator:->->:1202793..1203018 226 aatacaaactccgtttttggttagtaaataaattatatatcgcgcagattatacaacgatgagcgcaatataaaaatatttttgaacaagaaaagatcacaagattgcgtatgtgccaaacgatttatcggcgttcttatttagaacaaaatatagtatgatgtgcaaccgataaaccatgtcataaaacgatatattccacagcgaattttgaggggaaaaccac Intra-Species Hit: Count: 1 Min: 1 Max: 226 Len: 226 Subject: aact_AA01781_AA01782|molybdenum ABC transporter, periplasmic substrate-binding:molybdenum transport protein; transcriptional regulator|POSITIVE:POSITIVE|[1202793,1203018]|226 HSP 1 e-value: 1.0E-125 bit: 448.0 Len: 226 Query Start:1 Query End:226 Subject Strand: POSITIVE Subject Start: 1 Subject End: 226 aatacaaactccgtttttggttagtaaataaattatatatcgcgcagattatacaacgatgagcgcaatataaaaatatttttgaacaagaaaagatcacaagattgcgtatgtgccaaacgatttatcggcgttcttatttagaacaaaatatagtatgatgtgcaaccgataaaccatgtcataaaacgatatattccacagcgaattttgaggggaaaaccac aatacaaactccgtttttggttagtaaataaattatatatcgcgcagattatacaacgatgagcgcaatataaaaatatttttgaacaagaaaagatcacaagattgcgtatgtgccaaacgatttatcggcgttcttatttagaacaaaatatagtatgatgtgcaaccgataaaccatgtcataaaacgatatattccacagcgaattttgaggggaaaaccac Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUACAAACUCCGUUUUUGGUUAGUAAAUAAAUUAUAUAUCGCGCAGAUUAUACAACGAUGAGCGCAAUAUAAAAAUAUUUUUGAACAAGAAAAGAUCACAAGAUUGCGUAUGUGCCAAACGAUUUAUCGGCGUUCUUAUUUAGAACAAAAUAUAGUAUGAUGUGCAACCGAUAAACCAUGUCAUAAAACGAUAUAUUCCACAGCGAAUUUUGAGGGGAAAACCAC .........(((.(((..((((.((.........((((((((.(((.(((((((..(((((((((((((.........(((((......))))).........)))))))..((.....))...))))))..(((((.....)))))........))))))).)))....((((.....))))......))))))))......)).))))..))).)))....... (-47.63) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUGGUUUUCCCCUCAAAAUUCGCUGUGGAAUAUAUCGUUUUAUGACAUGGUUUAUCGGUUGCACAUCAUACUAUAUUUUGUUCUAAAUAAGAACGCCGAUAAAUCGUUUGGCACAUACGCAAUCUUGUGAUCUUUUCUUGUUCAAAAAUAUUUUUAUAUUGCGCUCAUCGUUGUAUAAUCUGCGCGAUAUAUAAUUUAUUUACUAACCAAAAACGGAGUUUGUAUU .(((((......(((......((((((..(((........)))..)))))).....((((((.................(((((.....)))))(((((........)))))......))))))...)))...............(((((..((((((((((((.................)))))))))).))..)))))...)))))................. (-42.33) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 283 218 178 153 99 aact:AA02132|5end_dihydroorotate_dehydrogenase_1455089..1455319_POSITIVE 283 137 100 61 28 aact:AA00377|5end_acetyl-CoA_acetyltransferase_(Beta-ketothiolase,3-ketoacyl-CoA_thiolase)_253042..253272_POSITIVE 274 143 113 140 117 aact:AA01515|5end_SlyX_family_protein_1020682..1020912_POSITIVE 265 134 94 45 1 aact:AA00516|5end_autoinducer-2_production_protein_355931..356161_POSITIVE 263 132 98 186 158 aact:AA00745|5end_galactokinase_511163..511393_POSITIVE 259 140 93 78 36 aact:AA00581|5end_thiosulfate_sulfurtransferase_(rhodanese)_397605..397835_POSITIVE 257 160 112 150 96 aact:AA02078|5end_hypothetical_protein_1412612..1412842_POSITIVE 255 206 173 138 99 aact:AA02939|5end_phosphoserine_phosphatase_2056881..2057111_POSITIVE 255 143 103 218 174 aact:AA01284|5end_heat_shock_protein_(hsp60)_868574..868804_POSITIVE 253 137 97 73 38 aact:AA02081|5end_periplasmic_protein_1414698..1414928_POSITIVE 251 58 42 179 163 aact:AA02344|5end_GTP-binding_protein,_elongation_factor_typA/bipA_1601966..1602196_POSITIVE 248 208 161 212 151 aact:AA01025|5end_hypothetical_protein_717522..717752_POSITIVE 247 136 95 160 118 aact:AA00894|5end_selenium_transferase_(Cysteinyl-tRNA(Sec)_selenium_transferase)_619879..620109_POSITIVE 245 138 98 75 24 aact:AA01393|5end_ABC_transporter,_ATP-binding_protein_940369..940599_POSITIVE 245 135 92 146 114 aact:AA00741|5end_possible_2-oxoglutarate/malate_transporter_506659..506889_POSITIVE 243 207 162 175 134 aact:AA02496|5end_hypothetical_protein_1731960..1732190_POSITIVE 243 142 107 54 19 aact:AA02043|5end_tagatose_6-phosphate_kinase_1390218..1390448_POSITIVE 243 132 101 43 11 aact:AA00927|5end_ATP-dependent_DNA_helicase_635811..636041_POSITIVE 241 133 112 134 115 aact:AA00575|5end_shikimate_5-dehydrogenase_393216..393446_POSITIVE 240 133 102 41 1 aact:AA01959|5end_tetraacyldisaccharide_4'-kinase_1323412..1323642_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 288 136 93 50 11 aact:AA01291|3end_excinuclease_ABC_subunit_A_876001..876151_POSITIVE 283 218 178 136 82 aact:AA02131|3end_conserved_hypothetical_protein_(possible_TrpH_protein)_1455152..1455302_POSITIVE 283 137 100 40 7 aact:AA00374|3end_short-chain_fatty_acids_transporter_253101..253251_POSITIVE 274 143 113 73 50 aact:AA01514|3end_peroxiredoxin_2_family_protein/glutaredoxin_1020695..1020845_POSITIVE 255 206 173 124 85 aact:AA02937|3end_conserved_hypothetical_protein_2056947..2057097_POSITIVE 255 143 103 94 50 aact:AA01280|3end_groES_chaperonin_868530..868680_POSITIVE 253 137 97 89 54 aact:AA02080|3end_ABC_transporter,_ATP-binding/permease_1414794..1414944_POSITIVE 243 207 162 127 86 aact:AA02495|3end_conserved_hypothetical_protein_1731992..1732142_POSITIVE 243 132 101 43 11 aact:AA00926|3end_CyaY_protein_635891..636041_POSITIVE 242 207 166 143 103 aact:AA01750|3end_MTA/SAH_nucleosidase_[Includes:_5'-methylthioadenosine_nucleosidase;_S-adenosylhomocysteine_nucleosidase]_1184519..1184669_POSITIVE 241 133 112 133 114 aact:AA00573|3end_chloramphenicol-sensitive_protein_RarD_393295..393445_POSITIVE 240 205 162 56 11 aact:AA00310|3end_conserved_hypothetical_protein_211514..211664_POSITIVE 239 133 94 80 45 aact:AA00530|3end_hypothetical_protein_362668..362818_POSITIVE 237 134 99 68 32 aact:AA01202|3end_integrase/recombinase_824207..824357_POSITIVE 235 119 101 151 131 aact:AA02623|3end_dTDP-D-glucose_4,6-dehydratase_1827979..1828129_POSITIVE 234 137 103 142 101 aact:AA01997|3end_probable_translation_factor_1356864..1357014_POSITIVE 232 135 106 44 15 aact:AA02573|3end_ABC_transporter,_ATP-binding_protein_1792429..1792579_POSITIVE 232 204 165 45 2 aact:AA02180|3end_conserved_hypothetical_protein_1490818..1490968_POSITIVE 232 149 106 147 92 aact:AA01394|3end_transcriptional_regulator_(mtr_efflux_pump)_943211..943361_POSITIVE 229 180 131 78 11 aact:AA02818|3end_ribosomal_RNA_small_subunit_methyltransferase_C_1981820..1981970_POSITIVE