Origin IGS: taaaataccgaagaaaatgaccgcactttgctctgtaccttaaaacttaggtttgttatctgtctaagttttgggggagagggtagagtgcttttcttttcagaaaaaatgcacctctatctaacctactaaataggggcgctcaaacta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagtttgagcgcccctatttagtaggttagatagaggtgcattttttctgaaaagaaaagcactctaccctctcccccaaaacttagacagataacaaacctaagttttaaggtacagagcaaagtgcggtcattttcttcggtatttta Mask Tandem Repeat Region ================================================ taaaataccgaagaaaatgaccgcactttgctctgtaccttaaaacttaggtttgttatctgtctaagttttgggggagagggtagagtgcttttcttttcagaaaaaatgcacctctatctaacctactaaataggggcgctcaaacta Find is-nt database================================================ Query_seq: AA01778:AA01779|AA01778:AA01779:aminopeptidase A/I; cytosol aminopeptidase; leucyl aminopeptidase:molybdenum ABC transporter, ATP-binding protein:->->:1200026..1200175 150 taaaataccgaagaaaatgaccgcactttgctctgtaccttaaaacttaggtttgttatctgtctaagttttgggggagagggtagagtgcttttcttttcagaaaaaatgcacctctatctaacctactaaataggggcgctcaaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01778:AA01779|AA01778:AA01779:aminopeptidase A/I; cytosol aminopeptidase; leucyl aminopeptidase:molybdenum ABC transporter, ATP-binding protein:->->:1200026..1200175 150 taaaataccgaagaaaatgaccgcactttgctctgtaccttaaaacttaggtttgttatctgtctaagttttgggggagagggtagagtgcttttcttttcagaaaaaatgcacctctatctaacctactaaataggggcgctcaaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01778:AA01779|AA01778:AA01779:aminopeptidase A/I; cytosol aminopeptidase; leucyl aminopeptidase:molybdenum ABC transporter, ATP-binding protein:->->:1200026..1200175 150 taaaataccgaagaaaatgaccgcactttgctctgtaccttaaaacttaggtttgttatctgtctaagttttgggggagagggtagagtgcttttcttttcagaaaaaatgcacctctatctaacctactaaataggggcgctcaaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaataccgaagaaaatgaccgcactttgctctgtaccttaaaacttaggtttgttatctgtctaagttttgggggagagggtagagtgcttttcttttcagaaaaaatgcacctctatctaacctactaaataggggcgctcaaacta Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 28 End: 129 ........................... M L C T L K L R F V I C L S F G G E G R V L F F S E K M H L Y L T Y ..................... Protein_Len: 36 Strand: - Start: 3 End: 110 .. F V S S F S R V K S Q V K F S L N T I Q R L K P P S P L T S K K E S F M ........................................ taaaataccgaagaaaatgaccgcactttgctctgtaccttaaaacttaggtttgttatctgtctaagttttgggggagagggtagagtgcttttcttttcagaaaaaatgcacctctatctaacctactaaataggggcgctcaaacta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaataccgaagaaaatgaccgcactttgctctgtaccttaaaacttaggtttgttatctgtctaagttttgggggagagggtagagtgcttttcttttcagaaaaaatgcacctctatctaacctactaaataggggcgctcaaacta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01778:AA01779|AA01778:AA01779:aminopeptidase A/I; cytosol aminopeptidase; leucyl aminopeptidase:molybdenum ABC transporter, ATP-binding protein:->->:1200026..1200175 150 taaaataccgaagaaaatgaccgcactttgctctgtaccttaaaacttaggtttgttatctgtctaagttttgggggagagggtagagtgcttttcttttcagaaaaaatgcacctctatctaacctactaaataggggcgctcaaacta Intra-Species Hit: Count: 1 Min: 1 Max: 150 Len: 150 Subject: aact_AA01778_AA01779|aminopeptidase A/I; cytosol aminopeptidase; leucyl aminopeptidase:molybdenum ABC transporter, ATP-binding protein|POSITIVE:POSITIVE|[1200026,1200175]|150 HSP 1 e-value: 5.0E-80 bit: 297.0 Len: 150 Query Start:1 Query End:150 Subject Strand: POSITIVE Subject Start: 1 Subject End: 150 taaaataccgaagaaaatgaccgcactttgctctgtaccttaaaacttaggtttgttatctgtctaagttttgggggagagggtagagtgcttttcttttcagaaaaaatgcacctctatctaacctactaaataggggcgctcaaacta taaaataccgaagaaaatgaccgcactttgctctgtaccttaaaacttaggtttgttatctgtctaagttttgggggagagggtagagtgcttttcttttcagaaaaaatgcacctctatctaacctactaaataggggcgctcaaacta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAUACCGAAGAAAAUGACCGCACUUUGCUCUGUACCUUAAAACUUAGGUUUGUUAUCUGUCUAAGUUUUGGGGGAGAGGGUAGAGUGCUUUUCUUUUCAGAAAAAAUGCACCUCUAUCUAACCUACUAAAUAGGGGCGCUCAAACUA .........((((((((.....((((((((((((.(.((((((((((((((...........)))))))))))))).).))))))))))))))))))))...........((.(((((((.((......)).))))))).))........ (-49.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUUUGAGCGCCCCUAUUUAGUAGGUUAGAUAGAGGUGCAUUUUUUCUGAAAAGAAAAGCACUCUACCCUCUCCCCCAAAACUUAGACAGAUAACAAACCUAAGUUUUAAGGUACAGAGCAAAGUGCGGUCAUUUUCUUCGGUAUUUUA ........(((((.((((((((....)))))))).))))).........(((((((...(((((.....((((.((..(((((((((.............)))))))))..))...))))...)))))..)).)))))............ (-37.92) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 466 51 18 142 108 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 444 80 31 127 61 aact:AA00145|5end_probable_degenerate_transposase_fragment_105046..105276_POSITIVE 421 34 2 138 106 aact:AA00454|5end_conserved_hypothetical_protein_318020..318250_POSITIVE 420 39 2 139 98 aact:AA01692|5end_hexulose-6-phosphate_synthase_1149178..1149408_POSITIVE 416 45 12 127 93 aact:AA00674|5end_nicotinate-nucleotide-dimethylbenzimidazole_phosphoribosyltransferase_461810..462040_POSITIVE 396 31 2 138 109 aact:AA01027|5end_Na(+)-translocating_NADH-quinone_reductase_subunit_F_717676..717906_POSITIVE 393 41 2 132 88 aact:AA02654|5end_probable_lipopolysaccharide_biosynthesis_protein_1848523..1848753_POSITIVE 387 68 21 196 141 aact:AA02010|5end_peptide_methionine_sulfoxide_reductase;_cytochrome_C-TYPE_biogenesis_protein;_transcriptional_regulator_1367180..1367410_POSITIVE 386 29 2 132 105 aact:AA00075|5end_conserved_hypothetical_protein_54963..55193_POSITIVE 363 46 1 136 92 aact:AA01818|5end_high-affinity_zinc_uptake_system_membrane_protein_1224306..1224536_POSITIVE 363 46 1 55 11 aact:AA01817|5end_hypothetical_protein_1224225..1224455_POSITIVE 361 35 2 140 107 aact:AA02963|5end_opacity_associated_protein_B_2072302..2072532_POSITIVE 361 45 2 130 88 aact:AA00536|5end_5'-nucleotidase_precursor_366151..366381_POSITIVE 356 80 32 119 80 aact:AA02115|5end_hypothetical_protein_1444528..1444758_POSITIVE 348 37 9 141 109 aact:AA00511|5end_nicotinamide_mononucleotide_transporter_353884..354114_POSITIVE 345 85 47 134 95 aact:AA00643|5end_hypothetical_protein_440432..440662_POSITIVE 344 38 2 153 109 aact:AA02131|5end_conserved_hypothetical_protein_(possible_TrpH_protein)_1454239..1454469_POSITIVE 342 60 14 186 136 aact:AA00386|5end_aspartate--ammonia_ligase_(asparagine_synthetase_A)_261150..261380_POSITIVE 335 44 9 158 120 aact:AA02410|5end_cell_division_protein_1655429..1655659_POSITIVE 333 32 2 124 93 aact:AA02106|5end_hypothetical_protein_1433241..1433471_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 466 51 18 81 47 aact:AA01289|3end_hypothetical_protein_872848..872998_POSITIVE 421 34 2 47 15 aact:AA00453|3end_phosphoglyceromutase_318009..318159_POSITIVE 396 31 2 151 122 aact:AA01025|3end_hypothetical_protein_717769..717919_POSITIVE 393 41 2 120 76 aact:AA02652|3end_conserved_hypothetical_protein_1848591..1848741_POSITIVE 386 29 2 36 9 aact:AA00072|3end_conserved_hypothetical_protein_54947..55097_POSITIVE 363 46 1 150 106 aact:AA01817|3end_hypothetical_protein_1224400..1224550_POSITIVE 361 35 2 43 10 aact:AA02962|3end_recombination_associated_protein_2072285..2072435_POSITIVE 356 80 32 81 42 aact:AA02114|3end_exodeoxyribonuclease_I_1444570..1444720_POSITIVE 355 58 12 90 39 aact:AA00010|3end_transposase_orfB_fragment_6297..6447_POSITIVE 348 37 9 60 28 aact:AA00508|3end_SAM-dependent_methyltransferase_353883..354033_POSITIVE 345 85 47 84 45 aact:AA00642|3end_conserved_hypothetical_protein_440462..440612_POSITIVE 344 38 2 135 91 aact:AA02130|3end_hypothetical_protein_1454301..1454451_POSITIVE 342 60 14 124 74 aact:AA00384|3end_conserved_hypothetical_protein_(possible_nitrogen_fixation_protein)_261168..261318_POSITIVE 335 44 9 53 15 aact:AA02409|3end_DNA_ligase_1655404..1655554_POSITIVE 333 32 2 93 62 aact:AA02105|3end_killing_protein_1433290..1433440_POSITIVE 331 91 61 46 16 aact:AA02338|3end_conserved_hypothetical_protein;_possible_integral_membrane_protein_1598885..1599035_POSITIVE 331 91 61 46 16 aact:AA01266|3end_hypothetical_protein_862251..862401_POSITIVE 331 91 61 66 36 aact:AA01074|3end_diacylglycerol_kinase_745623..745773_POSITIVE 328 80 32 62 16 aact:AA02418|3end_peptidyl-tRNA_hydrolase_1661811..1661961_POSITIVE 326 32 8 79 52 aact:AA00470|3end_conserved_hypothetical_protein_(possible_Zn-dependent_protease_with_chaperone_function)_327659..327809_POSITIVE