Origin IGS: ttttctcttgcaatttgagcaaaagagattaaattcgttgtaattaaaataggtgaatcacttgatttgcctaaataataagtaaggtatgataccctaatttagcatttaaaacagcaaaataagcgaggaaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttttcctcgcttattttgctgttttaaatgctaaattagggtatcataccttacttattatttaggcaaatcaagtgattcacctattttaattacaacgaatttaatctcttttgctcaaattgcaagagaaaa Mask Tandem Repeat Region ================================================ ttttctcttgcaatttgagcaaaagagattaaattcgttgtaattaaaataggtgaatcacttgatttgcctaaataataagtaaggtatgataccctaatttagcatttaaaacagcaaaataagcgaggaaaaa Find is-nt database================================================ Query_seq: AA01777:AA01778|AA01777:AA01778:conserved hypothetical protein:aminopeptidase A/I; cytosol aminopeptidase; leucyl aminopeptidase:->->:1198402..1198537 136 ttttctcttgcaatttgagcaaaagagattaaattcgttgtaattaaaataggtgaatcacttgatttgcctaaataataagtaaggtatgataccctaatttagcatttaaaacagcaaaataagcgaggaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01777:AA01778|AA01777:AA01778:conserved hypothetical protein:aminopeptidase A/I; cytosol aminopeptidase; leucyl aminopeptidase:->->:1198402..1198537 136 ttttctcttgcaatttgagcaaaagagattaaattcgttgtaattaaaataggtgaatcacttgatttgcctaaataataagtaaggtatgataccctaatttagcatttaaaacagcaaaataagcgaggaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01777:AA01778|AA01777:AA01778:conserved hypothetical protein:aminopeptidase A/I; cytosol aminopeptidase; leucyl aminopeptidase:->->:1198402..1198537 136 ttttctcttgcaatttgagcaaaagagattaaattcgttgtaattaaaataggtgaatcacttgatttgcctaaataataagtaaggtatgataccctaatttagcatttaaaacagcaaaataagcgaggaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttttctcttgcaatttgagcaaaagagattaaattcgttgtaattaaaataggtgaatcacttgatttgcctaaataataagtaaggtatgataccctaatttagcatttaaaacagcaaaataagcgaggaaaaa Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: - Start: 3 End: 101 .. E R A I Q A F S I L N T T I L I P S D S S K G L Y Y T L Y S V R M ................................... ttttctcttgcaatttgagcaaaagagattaaattcgttgtaattaaaataggtgaatcacttgatttgcctaaataataagtaaggtatgataccctaatttagcatttaaaacagcaaaataagcgaggaaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttttctcttgcaatttgagcaaaagagattaaattcgttgtaattaaaataggtgaatcacttgatttgcctaaataataagtaaggtatgataccctaatttagcatttaaaacagcaaaataagcgaggaaaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01777:AA01778|AA01777:AA01778:conserved hypothetical protein:aminopeptidase A/I; cytosol aminopeptidase; leucyl aminopeptidase:->->:1198402..1198537 136 ttttctcttgcaatttgagcaaaagagattaaattcgttgtaattaaaataggtgaatcacttgatttgcctaaataataagtaaggtatgataccctaatttagcatttaaaacagcaaaataagcgaggaaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 136 Len: 136 Subject: aact_AA01777_AA01778|conserved hypothetical protein:aminopeptidase A/I; cytosol aminopeptidase; leucyl aminopeptidase|POSITIVE:POSITIVE|[1198402,1198537]|136 HSP 1 e-value: 1.0E-71 bit: 270.0 Len: 136 Query Start:1 Query End:136 Subject Strand: POSITIVE Subject Start: 1 Subject End: 136 ttttctcttgcaatttgagcaaaagagattaaattcgttgtaattaaaataggtgaatcacttgatttgcctaaataataagtaaggtatgataccctaatttagcatttaaaacagcaaaataagcgaggaaaaa ttttctcttgcaatttgagcaaaagagattaaattcgttgtaattaaaataggtgaatcacttgatttgcctaaataataagtaaggtatgataccctaatttagcatttaaaacagcaaaataagcgaggaaaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUCUCUUGCAAUUUGAGCAAAAGAGAUUAAAUUCGUUGUAAUUAAAAUAGGUGAAUCACUUGAUUUGCCUAAAUAAUAAGUAAGGUAUGAUACCCUAAUUUAGCAUUUAAAACAGCAAAAUAAGCGAGGAAAAA (((((.(((((.((((((.(......).))))))..(((((........((((..((((....))))..)))).....(((((.(((........))).))))).........))))).......)))))))))). (-31.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUUCCUCGCUUAUUUUGCUGUUUUAAAUGCUAAAUUAGGGUAUCAUACCUUACUUAUUAUUUAGGCAAAUCAAGUGAUUCACCUAUUUUAAUUACAACGAAUUUAAUCUCUUUUGCUCAAAUUGCAAGAGAAAA .......((((((..((((((....((((((.(((..(((((((...))))))).))).))))))))))))..))))))...............................((((((((.......))))))))... (-28.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 300 79 32 90 32 aact:AA02983|5end_formate_acetyltransferase_2085195..2085425_POSITIVE 297 71 52 154 135 aact:AA00244|5end_conserved_hypothetical_protein_(possible_deoxyribonuclease,_possible_hydrolase)_172043..172273_POSITIVE 238 97 51 143 97 aact:AA00674|5end_nicotinate-nucleotide-dimethylbenzimidazole_phosphoribosyltransferase_461810..462040_POSITIVE 224 42 6 40 6 aact:AA01559|5end_RecA_regulatory_protein,_RecX_1045970..1046200_POSITIVE 220 130 81 170 124 aact:AA00875|5end_tight_adherence_protein_B_606877..607107_POSITIVE 218 108 63 160 120 aact:AA01460|5end_hypothetical_protein_981412..981642_POSITIVE 212 106 61 152 100 aact:AA00332|5end_PTS_system,_fructose-specific_IIA/FPr_component_(diphosphoryl_transfer_protein)_224011..224241_POSITIVE 211 68 25 231 190 aact:AA02389|5end_transcriptional_repressor_for_uxu_operon_1638528..1638758_POSITIVE 207 129 86 125 71 aact:AA02834|5end_colicin_transport_protein_1991269..1991499_POSITIVE 206 70 23 67 20 aact:AA00974|5end_glucosamine-fructose-6-phosphate_679215..679445_POSITIVE 205 62 21 104 62 aact:AA03011|5end_inner_membrane_protein,_60_kDa_2102585..2102815_POSITIVE 203 48 1 65 21 aact:AA00742|5end_hypothetical_protein_508185..508415_POSITIVE 202 97 51 225 185 aact:AA01165|5end_conserved_hypothetical_protein_798133..798363_POSITIVE 200 127 82 67 24 aact:AA02811|5end_hypothetical_protein_1979582..1979812_POSITIVE 200 99 51 152 101 aact:AA02324|5end_ABC_transporter_ATP_binding_protein_1586518..1586748_POSITIVE 198 44 1 119 79 aact:AA02419|5end_hypothetical_protein_1661731..1661961_POSITIVE 197 106 61 74 29 aact:AA01062|5end_ribonuclease_PH_(RNase_PH)_735706..735936_POSITIVE 196 28 8 202 180 aact:AA00253|5end_hypothetical_protein_177431..177661_POSITIVE 194 106 69 34 2 aact:AA02606|5end_ABC_transporter,_ATP-binding_protein/permease_1816725..1816955_POSITIVE 194 110 63 145 92 aact:AA01384|5end_hypothetical_protein_935320..935550_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 297 71 52 121 102 aact:AA00241|3end_formate_acetyltransferase_activating_enzyme_(anaerobic_ribonucleoside-triphosphate_reductase_activating_protein)_172090..172240_POSITIVE 207 129 86 105 51 aact:AA02832|3end_outer_membrane_integrity_protein_1991329..1991479_POSITIVE 198 44 1 119 79 aact:AA02418|3end_peptidyl-tRNA_hydrolase_1661811..1661961_POSITIVE 197 106 61 61 16 aact:AA01059|3end_orotate_phosphoribosyltransferase_735773..735923_POSITIVE 196 72 37 68 36 aact:AA01378|3end_glycerol-3-phosphatase_transporter_932872..933022_POSITIVE 196 93 52 52 1 aact:AA00619|3end_hypothetical_protein_424324..424474_POSITIVE 194 110 63 111 58 aact:AA01383|3end_DNA_processing_chain_A_935366..935516_POSITIVE 190 98 52 71 21 aact:AA02365|3end_hflC_protein_1622961..1623111_POSITIVE 190 44 6 90 53 aact:AA01538|3end_conserved_hypothetical_protein_1032734..1032884_POSITIVE 187 74 33 103 67 aact:AA00273|3end_fatty_acid/phospholipid_synthesis_protein_192499..192649_POSITIVE 185 41 8 132 95 aact:AA01511|3end_probable_transcriptional_regulator_1018900..1019050_POSITIVE 185 100 51 83 36 aact:AA00569|3end_ketol-acid_reductoisomerase_391582..391732_POSITIVE 179 40 4 132 91 aact:AA01325|3end_formamidopyrimidine-DNA_glycosylase_895639..895789_POSITIVE 179 91 51 59 14 aact:AA00845|3end_hypothetical_protein_584730..584880_POSITIVE 178 94 51 142 99 aact:AA01737|3end_exoribonuclease_II_1174703..1174853_POSITIVE 178 98 52 81 34 aact:AA00111|3end_hypothetical_protein_79986..80136_POSITIVE 176 54 15 43 2 aact:AA02647|3end_dTDP-D-glucose-4,6-dehydratase_1844437..1844587_POSITIVE 175 70 25 124 71 aact:AA02905|3end_conserved_hypothetical_protein_2033405..2033555_POSITIVE 175 125 81 68 22 aact:AA00414|3end_possible_histidinol-phosphatase_281533..281683_POSITIVE 172 130 81 74 24 aact:AA01129|3end_hypothetical_protein_774788..774938_POSITIVE