Origin IGS: taacggtttattttccctctgcattcccgccgcgtacgcgtttgccgtacgcggaaacctaattatccacacacttatctctctcattttcccgtatttttctcaaaaaaacacttgattctaaagttacttcatattttattatggtatcaaattttaattgataaaaggagagatcac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gtgatctctccttttatcaattaaaatttgataccataataaaatatgaagtaactttagaatcaagtgtttttttgagaaaaatacgggaaaatgagagagataagtgtgtggataattaggtttccgcgtacggcaaacgcgtacgcggcgggaatgcagagggaaaataaaccgtta Mask Tandem Repeat Region ================================================ taacggtttattttccctctgcattcccgccgcgtacgcgtttgccgtacgcggaaacctaattatccacacacttatctctctcattttcccgtatttttctcaaaaaaacacttgattctaaagttacttcatattttattatggtatcaaattttaattgataaaaggagagatcac Find is-nt database================================================ Query_seq: AA01750:AA01751|AA01750:AA01751:MTA/SAH nucleosidase [Includes: 5'-methylthioadenosine nucleosidase; S-adenosylhomocysteine nucleosidase]:conserved hypothetical protein:->->:1184580..1184759 180 taacggtttattttccctctgcattcccgccgcgtacgcgtttgccgtacgcggaaacctaattatccacacacttatctctctcattttcccgtatttttctcaaaaaaacacttgattctaaagttacttcatattttattatggtatcaaattttaattgataaaaggagagatcac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01750:AA01751|AA01750:AA01751:MTA/SAH nucleosidase [Includes: 5'-methylthioadenosine nucleosidase; S-adenosylhomocysteine nucleosidase]:conserved hypothetical protein:->->:1184580..1184759 180 taacggtttattttccctctgcattcccgccgcgtacgcgtttgccgtacgcggaaacctaattatccacacacttatctctctcattttcccgtatttttctcaaaaaaacacttgattctaaagttacttcatattttattatggtatcaaattttaattgataaaaggagagatcac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01750:AA01751|AA01750:AA01751:MTA/SAH nucleosidase [Includes: 5'-methylthioadenosine nucleosidase; S-adenosylhomocysteine nucleosidase]:conserved hypothetical protein:->->:1184580..1184759 180 taacggtttattttccctctgcattcccgccgcgtacgcgtttgccgtacgcggaaacctaattatccacacacttatctctctcattttcccgtatttttctcaaaaaaacacttgattctaaagttacttcatattttattatggtatcaaattttaattgataaaaggagagatcac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacggtttattttccctctgcattcccgccgcgtacgcgtttgccgtacgcggaaacctaattatccacacacttatctctctcattttcccgtatttttctcaaaaaaacacttgattctaaagttacttcatattttattatggtatcaaattttaattgataaaaggagagatcac Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 23 End: 115 ...................... M P A A Y A F A V R G N L I I H T L I S L I F P Y F S Q K N T ................................................................. Protein_Len: 57 Strand: + Start: 10 End: 180 ......... M F P L H S R R V R V C R T R K P N Y P H T Y L S H F P V F F S K K H L I L K L L H I L L W Y Q I L I D K R R D H taacggtttattttccctctgcattcccgccgcgtacgcgtttgccgtacgcggaaacctaattatccacacacttatctctctcattttcccgtatttttctcaaaaaaacacttgattctaaagttacttcatattttattatggtatcaaattttaattgataaaaggagagatcac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacggtttattttccctctgcattcccgccgcgtacgcgtttgccgtacgcggaaacctaattatccacacacttatctctctcattttcccgtatttttctcaaaaaaacacttgattctaaagttacttcatattttattatggtatcaaattttaattgataaaaggagagatcac Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01750:AA01751|AA01750:AA01751:MTA/SAH nucleosidase [Includes: 5'-methylthioadenosine nucleosidase; S-adenosylhomocysteine nucleosidase]:conserved hypothetical protein:->->:1184580..1184759 180 taacggtttattttccctctgcattcccgccgcgtacgcgtttgccgtacgcggaaacctaattatccacacacttatctctctcattttcccgtatttttctcaaaaaaacacttgattctaaagttacttcatattttattatggtatcaaattttaattgataaaaggagagatcac Intra-Species Hit: Count: 1 Min: 1 Max: 180 Len: 180 Subject: aact_AA01750_AA01751|MTA/SAH nucleosidase [Includes: 5'-methylthioadenosine nucleosidase; S-adenosylhomocysteine nucleosidase]:conserved hypothetical protein|POSITIVE:POSITIVE|[1184580,1184759]|180 HSP 1 e-value: 3.0E-85 bit: 315.0 Len: 180 Query Start:1 Query End:180 Subject Strand: POSITIVE Subject Start: 1 Subject End: 180 taacggtttattttccctctgcattcccgccgcgtacgcgtttgccgtacgcggaaacctaattatccacacacttatctctctcattttcccgtatttttctcnnnnnnncacttgattctaaagttacttcatattttattatggtatcaaattttaattgataaaaggagagatcac taacggtttattttccctctgcattcccgccgcgtacgcgtttgccgtacgcggaaacctaattatccacacacttatctctctcattttcccgtatttttctcaaaaaaacacttgattctaaagttacttcatattttattatggtatcaaattttaattgataaaaggagagatcac Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACGGUUUAUUUUCCCUCUGCAUUCCCGCCGCGUACGCGUUUGCCGUACGCGGAAACCUAAUUAUCCACACACUUAUCUCUCUCAUUUUCCCGUAUUUUUCUCAAAAAAACACUUGAUUCUAAAGUUACUUCAUAUUUUAUUAUGGUAUCAAAUUUUAAUUGAUAAAAGGAGAGAUCAC ....(((((....................(((((((((.(....))))))))))))))).................((((((((.......(((((......((((........))))...((((((........)))))).)))))((((((.......))))))...))))))))... (-34.75) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUGAUCUCUCCUUUUAUCAAUUAAAAUUUGAUACCAUAAUAAAAUAUGAAGUAACUUUAGAAUCAAGUGUUUUUUUGAGAAAAAUACGGGAAAAUGAGAGAGAUAAGUGUGUGGAUAAUUAGGUUUCCGCGUACGGCAAACGCGUACGCGGCGGGAAUGCAGAGGGAAAAUAAACCGUUA ...(((((((....((((((.......)))))).(((.........(((((...)))))...((..(((((((((...)))))))))..))..))).)))))))........((....(((..((((((((((((.......))))))).(((....)))...)))))..))).)).... (-38.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 429 54 16 111 72 aact:AA00329|5end_molybdopterin-guanine_dinucleotide_biosynthesis_protein_222737..222967_POSITIVE 407 60 13 159 105 aact:AA01957|5end_conserved_hypothetical_protein_1323211..1323441_POSITIVE 407 56 15 117 82 aact:AA01527|5end_hypothetical_protein_1025704..1025934_POSITIVE 405 59 11 204 144 aact:AA01338|5end_hypothetical_protein_904967..905197_POSITIVE 405 54 21 184 147 aact:AA00207|5end_D-ribose_ABC_transporter,_ATP-binding_protein_/_galactoside_ABC_transporter,_ATP-binding_protein_142592..142822_POSITIVE 395 59 15 201 134 aact:AA00894|5end_selenium_transferase_(Cysteinyl-tRNA(Sec)_selenium_transferase)_619879..620109_POSITIVE 391 46 4 89 37 aact:AA02076|5end_conserved_hypothetical_protein_1409926..1410156_POSITIVE 381 70 22 166 125 aact:AA02196|5end_oxaloacetate_decarboxylase_beta_chain_1496308..1496538_POSITIVE 380 54 20 75 42 aact:AA01760|5end_hydrogenase_isoenzymes_formation_protein_1189787..1190017_POSITIVE 377 70 26 63 12 aact:AA01776|5end_conserved_hypothetical_protein_1196074..1196304_POSITIVE 374 60 23 222 186 aact:AA01316|5end_hypothetical_protein_891262..891492_POSITIVE 373 59 13 89 37 aact:AA02088|5end_glycogen_operon_protein_1421282..1421512_POSITIVE 368 54 18 199 152 aact:AA00918|5end_hypothetical_protein_630044..630274_POSITIVE 365 59 19 89 46 aact:AA01224|5end_CTP_synthase_836221..836451_POSITIVE 365 47 18 181 151 aact:AA00805|5end_fumarate_reductase_iron-sulfur_protein_551583..551813_POSITIVE 365 54 12 176 135 aact:AA00042|5end_hypothetical_protein_33810..34040_POSITIVE 364 54 21 44 3 aact:AA00582|5end_conserved_hypothetical_protein_397904..398134_POSITIVE 363 80 31 101 48 aact:AA01234|5end_50S_ribosomal_protein_L18_841400..841630_POSITIVE 362 60 11 84 13 aact:AA01861|5end_phosphomethylpyrimidine_kinase_1251806..1252036_POSITIVE 362 69 21 195 129 aact:AA00993|5end_possible_D-tyrosyl-tRNA(Tyr)_deacylase_694488..694718_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 391 46 4 62 10 aact:AA02075|3end_conserved_hypothetical_protein_1409979..1410129_POSITIVE 381 55 13 150 100 aact:AA01956|3end_3-deoxy-manno-octulosonate_cytidylyltransferase_1323286..1323436_POSITIVE 380 54 20 72 39 aact:AA01759|3end_hydrogenase_isoenzymes_formation_protein_1189864..1190014_POSITIVE 377 70 26 91 40 aact:AA01775|3end_hypothetical_protein_1196182..1196332_POSITIVE 365 54 12 103 62 aact:AA00041|3end_cytochrome_c-type_biogenesis_protein_33817..33967_POSITIVE 363 80 31 84 31 aact:AA01233|3end_50S_ribosomal_protein_L6_841463..841613_POSITIVE 362 60 11 88 17 aact:AA01859|3end_hypothetical_protein_1251890..1252040_POSITIVE 356 52 18 147 107 aact:AA00267|3end_hypothetical_protein_188143..188293_POSITIVE 355 59 22 142 111 aact:AA02195|3end_oxaloacetate_decarboxylase_alpha_chain_1496374..1496524_POSITIVE 354 53 25 130 97 aact:AA00089|3end_S-adenosylmethionine_synthase;_methionine_adenosyltransferase_67749..67899_POSITIVE 350 54 11 122 80 aact:AA01690|3end_penicillin-binding_protein_1C_1149142..1149292_POSITIVE 349 60 17 142 91 aact:AA00974|3end_glucosamine-fructose-6-phosphate_681127..681277_POSITIVE 349 59 12 150 97 aact:AA00760|3end_conserved_hypothetical_protein_(possible_peptidase)_523815..523965_POSITIVE 341 53 23 82 51 aact:AA01954|3end_excinuclease_ABC,_subunit_C_1322314..1322464_POSITIVE 338 48 5 140 101 aact:AA00326|3end_periplasmic_oxidoreductase_(por)_(Thiol:disulfide_interchange_protein_dsbA_precursor.)_222397..222547_POSITIVE 337 41 4 144 111 aact:AA01151|3end_porphobilinogen_synthase_(delta-aminolevulinic_acid_dehydratase)_789422..789572_POSITIVE 335 59 26 147 110 aact:AA00196|3end_conserved_hypothetical_protein_138015..138165_POSITIVE 331 55 17 51 15 aact:AA01260|3end_hypothetical_protein_855758..855908_POSITIVE 330 54 30 61 37 aact:AA01750|3end_MTA/SAH_nucleosidase_[Includes:_5'-methylthioadenosine_nucleosidase;_S-adenosylhomocysteine_nucleosidase]_1184519..1184669_POSITIVE 329 57 14 58 9 aact:AA01422|3end_cell_division_protein_E,_ABC_system_ATP-binding_protein_956736..956886_POSITIVE