Origin IGS: actaagcctattaattcggttaatcgttatttaggcattgccgcccgaataaaattcaatcggtgatcacaaatgtgttttgaaaggagaagttgaaatgtcatgtgatacctagtttttttatgtaggaggtttgcgaactacagaaaaagcgatgaccttttcagatcatcacttttttcaaggttatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taataaccttgaaaaaagtgatgatctgaaaaggtcatcgctttttctgtagttcgcaaacctcctacataaaaaaactaggtatcacatgacatttcaacttctcctttcaaaacacatttgtgatcaccgattgaattttattcgggcggcaatgcctaaataacgattaaccgaattaataggcttagt Mask Tandem Repeat Region ================================================ actaagcctattaattcggttaatcgttatttaggcattgccgcccgaataaaattcaatcggtgatcacaaatgtgttttgaaaggagaagttgaaatgtcatgtgatacctagtttttttatgtaggaggtttgcgaactacagaaaaagcgatgaccttttcagatcatcacttttttcaaggttatta Find is-nt database================================================ Query_seq: AA01741:AA01743|AA01741:AA01743:6-pyruvoyl tetrahydrobiopterin synthase:lysyl-tRNA synthetase:->->:1177756..1177947 192 actaagcctattaattcggttaatcgttatttaggcattgccgcccgaataaaattcaatcggtgatcacaaatgtgttttgaaaggagaagttgaaatgtcatgtgatacctagtttttttatgtaggaggtttgcgaactacagaaaaagcgatgaccttttcagatcatcacttttttcaaggttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01741:AA01743|AA01741:AA01743:6-pyruvoyl tetrahydrobiopterin synthase:lysyl-tRNA synthetase:->->:1177756..1177947 192 actaagcctattaattcggttaatcgttatttaggcattgccgcccgaataaaattcaatcggtgatcacaaatgtgttttgaaaggagaagttgaaatgtcatgtgatacctagtttttttatgtaggaggtttgcgaactacagaaaaagcgatgaccttttcagatcatcacttttttcaaggttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01741:AA01743|AA01741:AA01743:6-pyruvoyl tetrahydrobiopterin synthase:lysyl-tRNA synthetase:->->:1177756..1177947 192 actaagcctattaattcggttaatcgttatttaggcattgccgcccgaataaaattcaatcggtgatcacaaatgtgttttgaaaggagaagttgaaatgtcatgtgatacctagtttttttatgtaggaggtttgcgaactacagaaaaagcgatgaccttttcagatcatcacttttttcaaggttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 actaagcctattaattcggttaatcgttatttaggcattgccgcccgaataaaattcaatcggtgatcacaaatgtgttttgaaaggagaagttgaaatgtcatgtgatacctagtttttttatgtaggaggtttgcgaactacagaaaaagcgatgaccttttcagatcatcacttttttcaaggttatta Predict ORF larger than 30AA ================================================ actaagcctattaattcggttaatcgttatttaggcattgccgcccgaataaaattcaatcggtgatcacaaatgtgttttgaaaggagaagttgaaatgtcatgtgatacctagtttttttatgtaggaggtttgcgaactacagaaaaagcgatgaccttttcagatcatcacttttttcaaggttatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ actaagcctattaattcggttaatcgttatttaggcattgccgcccgaataaaattcaatcggtgatcacaaatgtgttttgaaaggagaagttgaaatgtcatgtgatacctagtttttttatgtaggaggtttgcgaactacagaaaaagcgatgaccttttcagatcatcacttttttcaaggttatta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 72 HP_score: -3.8 Tail_Score: -5.06103 Start: 152 End: 175 Full_Region: CGAACTACAGAAAAA GCGATGACCT TTTC AGATCATCAC TTTTTTCAAGGTTAT .......................................................................................................................................................GCGATGACCTTTTCAGATCATCAC................. Find igs database================================================ Query_seq: AA01741:AA01743|AA01741:AA01743:6-pyruvoyl tetrahydrobiopterin synthase:lysyl-tRNA synthetase:->->:1177756..1177947 192 actaagcctattaattcggttaatcgttatttaggcattgccgcccgaataaaattcaatcggtgatcacaaatgtgttttgaaaggagaagttgaaatgtcatgtgatacctagtttttttatgtaggaggtttgcgaactacagaaaaagcgatgaccttttcagatcatcacttttttcaaggttatta Intra-Species Hit: Count: 1 Min: 1 Max: 192 Len: 192 Subject: aact_AA01741_AA01743|6-pyruvoyl tetrahydrobiopterin synthase:lysyl-tRNA synthetase|POSITIVE:POSITIVE|[1177756,1177947]|192 HSP 1 e-value: 2.0E-92 bit: 339.0 Len: 192 Query Start:1 Query End:192 Subject Strand: POSITIVE Subject Start: 1 Subject End: 192 actaagcctattaattcggttaatcgttatttaggcattgccgcccgaataaaattcaatcggtgatcacaaatgtgttttgaaaggagaagttgaaatgtcatgtgatacctagnnnnnnnatgtaggaggtttgcgaactacagaaaaagcgatgaccttttcagatcatcacttttttcaaggttatta actaagcctattaattcggttaatcgttatttaggcattgccgcccgaataaaattcaatcggtgatcacaaatgtgttttgaaaggagaagttgaaatgtcatgtgatacctagtttttttatgtaggaggtttgcgaactacagaaaaagcgatgaccttttcagatcatcacttttttcaaggttatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACUAAGCCUAUUAAUUCGGUUAAUCGUUAUUUAGGCAUUGCCGCCCGAAUAAAAUUCAAUCGGUGAUCACAAAUGUGUUUUGAAAGGAGAAGUUGAAAUGUCAUGUGAUACCUAGUUUUUUUAUGUAGGAGGUUUGCGAACUACAGAAAAAGCGAUGACCUUUUCAGAUCAUCACUUUUUUCAAGGUUAUUA ....(((((........((((.....(((((..(((......)))..))))).....))))(((((((.((((.....))))....((((((......((((....))))....((((((((.(((((............)))))))))))))......)))))).)))))))..........))))).... (-41.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAACCUUGAAAAAAGUGAUGAUCUGAAAAGGUCAUCGCUUUUUCUGUAGUUCGCAAACCUCCUACAUAAAAAAACUAGGUAUCACAUGACAUUUCAACUUCUCCUUUCAAAACACAUUUGUGAUCACCGAUUGAAUUUUAUUCGGGCGGCAAUGCCUAAAUAACGAUUAACCGAAUUAAUAGGCUUAGU ...((((((...(((((((((((((((....)))))))))))))))...(((((((........................(((((((((................................)))))).))).....(((((((((..((((....))))..))))).))))...)))))))..))).))).. (-44.75) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 363 53 14 197 148 aact:AA02306|5end_DNA-directed_RNA_polymerase,_omega_chain_1576947..1577177_POSITIVE 328 47 4 152 97 aact:AA01629|5end_phosphatidylglycerophosphatase_A_1101881..1102111_POSITIVE 300 65 23 69 17 aact:AA01162|5end_conserved_hypothetical_protein_(possible_transmembrane_protein)_795073..795303_POSITIVE 297 50 9 107 70 aact:AA02676|5end_formate_dehydrogenase_alpha_subunit_1861671..1861901_POSITIVE 295 68 29 83 30 aact:AA01861|5end_phosphomethylpyrimidine_kinase_1251806..1252036_POSITIVE 294 128 81 176 127 aact:AA01318|5end_hypothetical_protein_892082..892312_POSITIVE 293 70 24 158 107 aact:AA01813|5end_electron_transport_complex_protein_1220949..1221179_POSITIVE 291 48 6 230 190 aact:AA01417|5end_probable_4-diphosphocytidyl-2C-methyl-D-erthyritol_synthase_(MEP_cytidyltransferase)_(MCT)_953256..953486_POSITIVE 289 65 34 229 202 aact:AA02927|5end_hypothetical_protein_2048233..2048463_POSITIVE 289 65 34 132 105 aact:AA02926|5end_hypothetical_protein_2048136..2048366_POSITIVE 284 69 25 75 34 aact:AA01715|5end_conserved_hypothetical_protein_1160480..1160710_POSITIVE 284 47 17 34 10 aact:AA00705|5end_Inositol-1-monophosphatase_(IMPase);_extragenic_suppressor_483827..484057_POSITIVE 283 75 33 115 48 aact:AA01692|5end_hexulose-6-phosphate_synthase_1149178..1149408_POSITIVE 282 48 2 50 3 aact:AA01938|5end_NAD(P)_transhydrogenase_subunit_alpha_1309493..1309723_POSITIVE 281 63 21 127 85 aact:AA00492|5end_conserved_hypothetical_protein_343578..343808_POSITIVE 278 47 1 54 18 aact:AA02402|5end_O-sialoglycoprotein_endopeptidase_1651337..1651567_POSITIVE 276 64 25 194 155 aact:AA02847|5end_conserved_hypothetical_protein_1999276..1999506_POSITIVE 274 49 4 64 6 aact:AA01416|5end_2C-methyl-D-erythritol_2,4-cyclodiphosphate_952780..953010_POSITIVE 274 65 22 196 157 aact:AA01125|5end_hypothetical_protein_772192..772422_POSITIVE 272 109 61 70 22 aact:AA02552|5end_iron_(chelated)_ABC_transporter,_permease_protein_1778495..1778725_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 363 53 14 148 99 aact:AA02305|3end_guanosine-3',5'-bis(diphosphate)3'-_pyrophosphohydrolase_1576978..1577128_POSITIVE 328 47 4 139 84 aact:AA01628|3end_thiamin-monophosphate_kinase_1101948..1102098_POSITIVE 297 50 9 55 18 aact:AA02675|3end_formate_dehydrogenase_alpha_subunit_1861699..1861849_POSITIVE 295 68 29 87 34 aact:AA01859|3end_hypothetical_protein_1251890..1252040_POSITIVE 289 65 34 67 40 aact:AA02925|3end_conserved_hypothetical_protein_2048151..2048301_POSITIVE 276 64 25 135 96 aact:AA02846|3end_crossover_junction_endodeoxyribonuclease_1999297..1999447_POSITIVE 274 49 4 64 6 aact:AA01413|3end_conserved_hypothetical_protein_952860..953010_POSITIVE 272 109 61 70 22 aact:AA02551|3end_iron_(chelated)_ABC_transporter,_permease_protein_1778575..1778725_POSITIVE 266 65 25 133 86 aact:AA01374|3end_tRNA/rRNA_methyltransferase_931097..931247_POSITIVE 266 80 31 115 62 aact:AA00708|3end_cysteine_desulfurase_487395..487545_POSITIVE 265 90 41 84 32 aact:AA01216|3end_conserved_hypothetical_protein_832709..832859_POSITIVE 262 67 33 73 33 aact:AA02340|3end_hypothetical_protein_1599341..1599491_POSITIVE 262 67 33 81 41 aact:AA02115|3end_hypothetical_protein_1444991..1445141_POSITIVE 262 67 33 81 41 aact:AA01075|3end_hypothetical_protein_746068..746218_POSITIVE 262 48 5 80 40 aact:AA01066|3end_conserved_hypothetical_protein_738900..739050_POSITIVE 262 47 1 66 28 aact:AA01053|3end_conserved_hypothetical_protein_733747..733897_POSITIVE 262 66 33 140 96 aact:AA00988|3end_conserved_hypothetical_protein_692264..692414_POSITIVE 262 67 33 81 41 aact:AA00008|3end_hypothetical_protein_(possible_transposase_fragment)_5495..5645_POSITIVE 256 42 8 68 35 aact:AA00025|3end_hypothetical_protein_19986..20136_POSITIVE 254 79 37 53 12 aact:AA00318|3end_hypothetical_protein_219049..219199_POSITIVE