Origin IGS: taattacatttaatcaaatataaggttcaaaaatgaaagttgttattctaattttggctatatttctttcatttattttttaaagtaaaagaaacaagaagaaaaataaaattttcactacttatcaaccgcaagaattttcagagaaagagaaggaaaatatacgacaaaaactaattctatctacagatttcaaaacaataccaata .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tattggtattgttttgaaatctgtagatagaattagtttttgtcgtatattttccttctctttctctgaaaattcttgcggttgataagtagtgaaaattttatttttcttcttgtttcttttactttaaaaaataaatgaaagaaatatagccaaaattagaataacaactttcatttttgaaccttatatttgattaaatgtaatta Mask Tandem Repeat Region ================================================ taattacatttaatcaaatataaggttcaaaaatgaaagttgttattctaattttggctatatttctttcatttattttttaaagtaaaagaaacaagaagaaaaataaaattttcactacttatcaaccgcaagaattttcagagaaagagaaggaaaatatacgacaaaaactaattctatctacagatttcaaaacaataccaata Find is-nt database================================================ Query_seq: AA01722:AA01723|AA01722:AA01723:conserved hypothetical protein:conserved hypothetical protein:->->:1163700..1163908 209 taattacatttaatcaaatataaggttcaaaaatgaaagttgttattctaattttggctatatttctttcatttattttttaaagtaaaagaaacaagaagaaaaataaaattttcactacttatcaaccgcaagaattttcagagaaagagaaggaaaatatacgacaaaaactaattctatctacagatttcaaaacaataccaata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01722:AA01723|AA01722:AA01723:conserved hypothetical protein:conserved hypothetical protein:->->:1163700..1163908 209 taattacatttaatcaaatataaggttcaaaaatgaaagttgttattctaattttggctatatttctttcatttattttttaaagtaaaagaaacaagaagaaaaataaaattttcactacttatcaaccgcaagaattttcagagaaagagaaggaaaatatacgacaaaaactaattctatctacagatttcaaaacaataccaata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01722:AA01723|AA01722:AA01723:conserved hypothetical protein:conserved hypothetical protein:->->:1163700..1163908 209 taattacatttaatcaaatataaggttcaaaaatgaaagttgttattctaattttggctatatttctttcatttattttttaaagtaaaagaaacaagaagaaaaataaaattttcactacttatcaaccgcaagaattttcagagaaagagaaggaaaatatacgacaaaaactaattctatctacagatttcaaaacaataccaata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taattacatttaatcaaatataaggttcaaaaatgaaagttgttattctaattttggctatatttctttcatttattttttaaagtaaaagaaacaagaagaaaaataaaattttcactacttatcaaccgcaagaattttcagagaaagagaaggaaaatatacgacaaaaactaattctatctacagatttcaaaacaataccaata Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 111 End: 209 .............................................................................................................. M F T T Y Q P Q E F S E K E K E N I R Q K L I L S T D F K T I P I taattacatttaatcaaatataaggttcaaaaatgaaagttgttattctaattttggctatatttctttcatttattttttaaagtaaaagaaacaagaagaaaaataaaattttcactacttatcaaccgcaagaattttcagagaaagagaaggaaaatatacgacaaaaactaattctatctacagatttcaaaacaataccaata Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taattacatttaatcaaatataaggttcaaaaatgaaagttgttattctaattttggctatatttctttcatttattttttaaagtaaaagaaacaagaagaaaaataaaattttcactacttatcaaccgcaagaattttcagagaaagagaaggaaaatatacgacaaaaactaattctatctacagatttcaaaacaataccaata Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01722:AA01723|AA01722:AA01723:conserved hypothetical protein:conserved hypothetical protein:->->:1163700..1163908 209 taattacatttaatcaaatataaggttcaaaaatgaaagttgttattctaattttggctatatttctttcatttattttttaaagtaaaagaaacaagaagaaaaataaaattttcactacttatcaaccgcaagaattttcagagaaagagaaggaaaatatacgacaaaaactaattctatctacagatttcaaaacaataccaata Intra-Species Hit: Count: 1 Min: 1 Max: 209 Len: 209 Subject: aact_AA01722_AA01723|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1163700,1163908]|209 HSP 1 e-value: 1.0E-115 bit: 414.0 Len: 209 Query Start:1 Query End:209 Subject Strand: POSITIVE Subject Start: 1 Subject End: 209 taattacatttaatcaaatataaggttcaaaaatgaaagttgttattctaattttggctatatttctttcatttattttttaaagtaaaagaaacaagaagaaaaataaaattttcactacttatcaaccgcaagaattttcagagaaagagaaggaaaatatacgacaaaaactaattctatctacagatttcaaaacaataccaata taattacatttaatcaaatataaggttcaaaaatgaaagttgttattctaattttggctatatttctttcatttattttttaaagtaaaagaaacaagaagaaaaataaaattttcactacttatcaaccgcaagaattttcagagaaagagaaggaaaatatacgacaaaaactaattctatctacagatttcaaaacaataccaata Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUACAUUUAAUCAAAUAUAAGGUUCAAAAAUGAAAGUUGUUAUUCUAAUUUUGGCUAUAUUUCUUUCAUUUAUUUUUUAAAGUAAAAGAAACAAGAAGAAAAAUAAAAUUUUCACUACUUAUCAACCGCAAGAAUUUUCAGAGAAAGAGAAGGAAAAUAUACGACAAAAACUAAUUCUAUCUACAGAUUUCAAAACAAUACCAAUA .......................(((.......(((((.((((........(((((..((((((((((((.(((.(((((.(((((..............(((((......))))).............(....).))))).))))).)))))))).)))))))...))))).............)))).))))).......))).... (-23.84) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUUGGUAUUGUUUUGAAAUCUGUAGAUAGAAUUAGUUUUUGUCGUAUAUUUUCCUUCUCUUUCUCUGAAAAUUCUUGCGGUUGAUAAGUAGUGAAAAUUUUAUUUUUCUUCUUGUUUCUUUUACUUUAAAAAAUAAAUGAAAGAAAUAUAGCCAAAAUUAGAAUAACAACUUUCAUUUUUGAACCUUAUAUUUGAUUAAAUGUAAUUA ..(((((........((((......(((((((.....)))))))....((((((((.((..((..(((..........)))..))..)).)).))))))......))))....(((((((((((.((((.....)))))))))))))))..)))))................((((....))))..((((((((....))))))))... (-29.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 268 65 23 161 119 aact:AA02832|5end_outer_membrane_integrity_protein_1990044..1990274_POSITIVE 218 169 121 227 175 aact:AA01933|5end_hypothetical_protein_1306060..1306290_POSITIVE 218 156 116 229 201 aact:AA01379|5end_glycerophosphoryl_diester_phosphodiesterase_(protein_D)_933029..933259_POSITIVE 215 158 126 85 53 aact:AA01154|5end_sec-independent_protein_translocase_protein_790832..791062_POSITIVE 202 169 122 63 9 aact:AA02998|5end_conserved_hypothetical_protein_2093707..2093937_POSITIVE 202 72 40 141 104 aact:AA02516|5end_ferric_uptake_regulation_protein_1749264..1749494_POSITIVE 201 169 128 226 178 aact:AA00113|5end_uracil_phosphoribosyltransferase_81286..81516_POSITIVE 200 199 151 94 42 aact:AA02005|5end_vancomycin_sensitivity;_SanA_protein_1363143..1363373_POSITIVE 200 156 129 206 181 aact:AA01391|5end_glycerol-3-phosphate_acyltransferase_937887..938117_POSITIVE 199 136 92 60 5 aact:AA02001|5end_conserved_hypothetical_protein_1359842..1360072_POSITIVE 197 157 128 176 150 aact:AA00766|5end_heat_shock_protein_B25.3_(HSP-70_cofactor)_527384..527614_POSITIVE 195 168 126 197 162 aact:AA02504|5end_lipid_A_biosynthesis_(kdo)2-(lauroyl)-lipid_IVA_acyltransferase_1738215..1738445_POSITIVE 187 156 121 116 76 aact:AA01515|5end_SlyX_family_protein_1020682..1020912_POSITIVE 187 169 130 74 38 aact:AA01288|5end_hypothetical_protein_872086..872316_POSITIVE 186 155 117 135 96 aact:AA00876|5end_tight_adherence_protein_C_607761..607991_POSITIVE 184 161 128 228 190 aact:AA00652|5end_arginyl-tRNA_synthetase_(arginine--tRNA_ligase)_445718..445948_POSITIVE 183 148 112 174 130 aact:AA01359|5end_conserved_hypothetical_protein_920484..920714_POSITIVE 180 155 111 148 100 aact:AA01565|5end_conserved_hypothetical_protein_1050711..1050941_POSITIVE 180 45 6 101 64 aact:AA00884|5end_sodium-dependent_transporter_614392..614622_POSITIVE 179 75 33 70 27 aact:AA01385|5end_50S_ribosomal_protein_L9_935509..935739_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 268 65 23 123 81 aact:AA02830|3end_colicin_tolerance_protein_1990086..1990236_POSITIVE 215 158 126 78 46 aact:AA01153|3end_sec-independent_protein_translocase_790905..791055_POSITIVE 214 152 119 129 85 aact:AA01280|3end_groES_chaperonin_868530..868680_POSITIVE 202 169 122 60 6 aact:AA02996|3end_probable_cation_transport_protein_2093784..2093934_POSITIVE 202 72 40 119 82 aact:AA02515|3end_flavodoxin_1749322..1749472_POSITIVE 201 169 128 99 51 aact:AA00112|3end_uracil_permease_81239..81389_POSITIVE 197 157 128 59 33 aact:AA00764|3end_hemoglobin_binding_protein_A_527347..527497_POSITIVE 189 60 30 74 48 aact:AA01895|3end_hypothetical_protein_1280601..1280751_POSITIVE 189 74 34 141 98 aact:AA01715|3end_conserved_hypothetical_protein_1161162..1161312_POSITIVE 187 156 121 49 9 aact:AA01514|3end_peroxiredoxin_2_family_protein/glutaredoxin_1020695..1020845_POSITIVE 187 169 130 77 41 aact:AA01287|3end_transposase_872169..872319_POSITIVE 186 155 117 135 96 aact:AA00875|3end_tight_adherence_protein_B_607841..607991_POSITIVE 184 161 128 89 51 aact:AA00651|3end_conserved_hypothetical_protein_445659..445809_POSITIVE 180 45 6 96 59 aact:AA00883|3end_hypothetical_protein_614467..614617_POSITIVE 177 156 111 83 42 aact:AA00998|3end_small_protein_A_homolog_699015..699165_POSITIVE 176 58 23 41 13 aact:AA02918|3end_hypothetical_protein_2043501..2043651_POSITIVE 175 135 106 53 25 aact:AA00978|3end_conserved_hypothetical_protein_684585..684735_POSITIVE 173 185 146 42 5 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 173 155 129 28 1 aact:AA01942|3end_hypothetical_protein_1312953..1313103_POSITIVE 173 144 108 138 108 aact:AA01923|3end_type_III_restriction-modification_system_methylation_subunit_1299936..1300086_POSITIVE