Origin IGS: taaaacggaaccgtgggaggatttcagtgaagcgaaaatctcggaggaatgtagcttttccgatggtgcggcgcatgaagttcagtgataattatttgacccgcgcgtttggtggcggaaaattattgttcggggtttgagcaaattattctaacggaacaaattgcactatttttttatcatgcagaaaaatctaaatgttttttccaccgcacttttttattaagcgaaatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatttcgcttaataaaaaagtgcggtggaaaaaacatttagatttttctgcatgataaaaaaatagtgcaatttgttccgttagaataatttgctcaaaccccgaacaataattttccgccaccaaacgcgcgggtcaaataattatcactgaacttcatgcgccgcaccatcggaaaagctacattcctccgagattttcgcttcactgaaatcctcccacggttccgtttta Mask Tandem Repeat Region ================================================ taaaacggaaccgtgggaggatttcagtgaagcgaaaatctcggaggaatgtagcttttccgatggtgcggcgcatgaagttcagtgataattatttgacccgcgcgtttggtggcggaaaattattgttcggggtttgagcaaattattctaacggaacaaattgcactatttttttatcatgcagaaaaatctaaatgttttttccaccgcacttttttattaagcgaaatta Find is-nt database================================================ Query_seq: AA01705:AA01708|AA01705:AA01708:beta-galactosidase:glutaredoxin:->->:1158041..1158275 235 taaaacggaaccgtgggaggatttcagtgaagcgaaaatctcggaggaatgtagcttttccgatggtgcggcgcatgaagttcagtgataattatttgacccgcgcgtttggtggcggaaaattattgttcggggtttgagcaaattattctaacggaacaaattgcactatttttttatcatgcagaaaaatctaaatgttttttccaccgcacttttttattaagcgaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01705:AA01708|AA01705:AA01708:beta-galactosidase:glutaredoxin:->->:1158041..1158275 235 taaaacggaaccgtgggaggatttcagtgaagcgaaaatctcggaggaatgtagcttttccgatggtgcggcgcatgaagttcagtgataattatttgacccgcgcgtttggtggcggaaaattattgttcggggtttgagcaaattattctaacggaacaaattgcactatttttttatcatgcagaaaaatctaaatgttttttccaccgcacttttttattaagcgaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01705:AA01708|AA01705:AA01708:beta-galactosidase:glutaredoxin:->->:1158041..1158275 235 taaaacggaaccgtgggaggatttcagtgaagcgaaaatctcggaggaatgtagcttttccgatggtgcggcgcatgaagttcagtgataattatttgacccgcgcgtttggtggcggaaaattattgttcggggtttgagcaaattattctaacggaacaaattgcactatttttttatcatgcagaaaaatctaaatgttttttccaccgcacttttttattaagcgaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaacggaaccgtgggaggatttcagtgaagcgaaaatctcggaggaatgtagcttttccgatggtgcggcgcatgaagttcagtgataattatttgacccgcgcgtttggtggcggaaaattattgttcggggtttgagcaaattattctaacggaacaaattgcactatttttttatcatgcagaaaaatctaaatgttttttccaccgcacttttttattaagcgaaatta Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 21 End: 137 .................... M S V K R K S R R N V A F P M V R R M K F S D N Y L T R A F G G G K L L F G V .................................................................................................. Protein_Len: 42 Strand: + Start: 109 End: 234 ............................................................................................................ M V A E N Y C S G F E Q I I L T E Q I A L F F Y H A E K S K C F F H R T F L L S E I . taaaacggaaccgtgggaggatttcagtgaagcgaaaatctcggaggaatgtagcttttccgatggtgcggcgcatgaagttcagtgataattatttgacccgcgcgtttggtggcggaaaattattgttcggggtttgagcaaattattctaacggaacaaattgcactatttttttatcatgcagaaaaatctaaatgttttttccaccgcacttttttattaagcgaaatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaacggaaccgtgggaggatttcagtgaagcgaaaatctcggaggaatgtagcttttccgatggtgcggcgcatgaagttcagtgataattatttgacccgcgcgtttggtggcggaaaattattgttcggggtttgagcaaattattctaacggaacaaattgcactatttttttatcatgcagaaaaatctaaatgttttttccaccgcacttttttattaagcgaaatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01705:AA01708|AA01705:AA01708:beta-galactosidase:glutaredoxin:->->:1158041..1158275 235 taaaacggaaccgtgggaggatttcagtgaagcgaaaatctcggaggaatgtagcttttccgatggtgcggcgcatgaagttcagtgataattatttgacccgcgcgtttggtggcggaaaattattgttcggggtttgagcaaattattctaacggaacaaattgcactatttttttatcatgcagaaaaatctaaatgttttttccaccgcacttttttattaagcgaaatta Intra-Species Hit: Count: 1 Min: 1 Max: 235 Len: 235 Subject: aact_AA01705_AA01708|beta- galactosidase:glutaredoxin|POSITIVE:POSITIVE|[1158041, 1158275]|235 HSP 1 e-value: 1.0E-118 bit: 424.0 Len: 235 Query Start:1 Query End:235 Subject Strand: POSITIVE Subject Start: 1 Subject End: 235 taaaacggaaccgtgggaggatttcagtgaagcgaaaatctcggaggaatgtagcttttccgatggtgcggcgcatgaagttcagtgataattatttgacccgcgcgtttggtggcggaaaattattgttcggggtttgagcaaattattctaacggaacaaattgcactannnnnnnatcatgcagaaaaatctaaatgttttttccaccgcacttttttattaagcgaaatta taaaacggaaccgtgggaggatttcagtgaagcgaaaatctcggaggaatgtagcttttccgatggtgcggcgcatgaagttcagtgataattatttgacccgcgcgtttggtggcggaaaattattgttcggggtttgagcaaattattctaacggaacaaattgcactatttttttatcatgcagaaaaatctaaatgttttttccaccgcacttttttattaagcgaaatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAACGGAACCGUGGGAGGAUUUCAGUGAAGCGAAAAUCUCGGAGGAAUGUAGCUUUUCCGAUGGUGCGGCGCAUGAAGUUCAGUGAUAAUUAUUUGACCCGCGCGUUUGGUGGCGGAAAAUUAUUGUUCGGGGUUUGAGCAAAUUAUUCUAACGGAACAAAUUGCACUAUUUUUUUAUCAUGCAGAAAAAUCUAAAUGUUUUUUCCACCGCACUUUUUUAUUAAGCGAAAUUA .......((((((((((.(((((((((((..(((....(.((((((((.......)))))))).).)))..))).)))))))).....(((....))).)))))).)))).((((.((((((...((((((.(((..(((.....)))..))..).)))))).(((((..((......))..)))))...............)))))).))))...................... (-57.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUUCGCUUAAUAAAAAAGUGCGGUGGAAAAAACAUUUAGAUUUUUCUGCAUGAUAAAAAAAUAGUGCAAUUUGUUCCGUUAGAAUAAUUUGCUCAAACCCCGAACAAUAAUUUUCCGCCACCAAACGCGCGGGUCAAAUAAUUAUCACUGAACUUCAUGCGCCGCACCAUCGGAAAAGCUACAUUCCUCCGAGAUUUUCGCUUCACUGAAAUCCUCCCACGGUUCCGUUUUA ....................(((((((((((((..(((.((((....)))).)))...........(.((((.((((((.....)))))).)))).)................))))))))).......(((((..(((............)))......))))).))))....((((........))))...(((..(((((......)))))..)))..(((....))).... (-47.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 430 145 101 217 179 aact:AA01401|5end_3-deoxy-D-manno-octulosonic-acid_transferase_946165..946395_POSITIVE 420 80 32 68 17 aact:AA01648|5end_alpha-amylase_protein;_1,4-alpha-D-glucan_glucanohydrolase_1115170..1115400_POSITIVE 388 87 42 161 119 aact:AA00866|5end_conserved_hypothetical_protein_599925..600155_POSITIVE 388 228 183 152 105 aact:AA00497|5end_hexose_phosphate_transport_system_regulatory_protein;_regulator_of_uhpT_expression_345077..345307_POSITIVE 383 133 97 229 191 aact:AA01313|5end_deoxyuridinetriphosphatase;_deoxyuridine_5'-triphosphate_nucleotidohydrolase_889567..889797_POSITIVE 381 85 41 82 48 aact:AA02470|5end_glucose_kinase_1706730..1706960_POSITIVE 380 139 101 219 179 aact:AA02590|5end_conserved_hypothetical_protein_1801113..1801343_POSITIVE 374 149 101 92 32 aact:AA01188|5end_conserved_hypothetical_protein_813912..814142_POSITIVE 370 146 102 207 146 aact:AA00381|5end_ornithine_carbamoyltransferase_257470..257700_POSITIVE 368 140 102 224 182 aact:AA01152|5end_sec-independent_protein_translocase_protein_789364..789594_POSITIVE 365 149 103 76 22 aact:AA00259|5end_conserved_hypothetical_protein_182788..183018_POSITIVE 363 143 101 39 1 aact:AA01609|5end_p-aminobenzoate_synthase_component_I_homolog_1087903..1088133_POSITIVE 358 120 71 123 81 aact:AA01724|5end_valyl-tRNA_synthetase_1164446..1164676_POSITIVE 357 135 99 82 38 aact:AA01417|5end_probable_4-diphosphocytidyl-2C-methyl-D-erthyritol_synthase_(MEP_cytidyltransferase)_(MCT)_953256..953486_POSITIVE 357 77 31 145 100 aact:AA00012|5end_conserved_hypothetical_protein_6433..6663_POSITIVE 354 119 72 90 46 aact:AA02980|5end_conserved_hypothetical_protein_2083305..2083535_POSITIVE 352 148 101 80 22 aact:AA01692|5end_hexulose-6-phosphate_synthase_1149178..1149408_POSITIVE 348 143 101 167 119 aact:AA02567|5end_hypothetical_protein_(possible_inner_membrane_protein)_1788225..1788455_POSITIVE 348 135 95 162 120 aact:AA01794|5end_glycine_cleavage_system_transcription_activator_1209708..1209938_POSITIVE 345 106 61 213 170 aact:AA00803|5end_hypothetical_protein_549526..549756_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 420 80 32 80 29 aact:AA01646|3end_hypothetical_protein_1115262..1115412_POSITIVE 388 228 183 134 87 aact:AA00495|3end_hypothetical_protein_345139..345289_POSITIVE 381 85 41 69 35 aact:AA02468|3end_conserved_hypothetical_protein_1706797..1706947_POSITIVE 374 149 101 92 32 aact:AA01187|3end_conserved_hypothetical_protein_813992..814142_POSITIVE 365 149 103 60 6 aact:AA00258|3end_penicillin_binding_protein_2_182852..183002_POSITIVE 357 135 99 82 38 aact:AA01416|3end_2C-methyl-D-erythritol_2,4-cyclodiphosphate_953336..953486_POSITIVE 345 106 61 146 103 aact:AA00801|3end_lysine--tRNA_ligase_549539..549689_POSITIVE 337 119 84 50 17 aact:AA02965|3end_hypothetical_protein_2074286..2074436_POSITIVE 333 120 73 145 97 aact:AA02989|3end_conserved_hypothetical_protein_2089548..2089698_POSITIVE 332 219 183 84 46 aact:AA02402|3end_O-sialoglycoprotein_endopeptidase_1652442..1652592_POSITIVE 331 133 101 65 32 aact:AA00288|3end_conserved_hypothetical_protein_201347..201497_POSITIVE 324 135 101 135 95 aact:AA01428|3end_acetolactate_synthase_959625..959775_POSITIVE 320 136 97 85 42 aact:AA02805|3end_leukotoxin_expression_protein_B;_hemolysin_secretion_protein_1973930..1974080_POSITIVE 320 87 41 148 107 aact:AA00760|3end_conserved_hypothetical_protein_(possible_peptidase)_523815..523965_POSITIVE 319 145 101 100 60 aact:AA02225|3end_ABC_transporter_ATP-binding_protein_1514592..1514742_POSITIVE 318 75 32 62 1 aact:AA01358|3end_conserved_hypothetical_protein_920387..920537_POSITIVE 318 106 61 74 34 aact:AA00896|3end_prolyl-tRNA_synthetase_(proline-tRNA_ligase)_623206..623356_POSITIVE 316 59 12 139 100 aact:AA01003|3end_conserved_hypothetical_protein_700454..700604_POSITIVE 311 70 22 65 18 aact:AA00345|3end_transcription_antitermination_protein_232058..232208_POSITIVE 310 228 189 87 53 aact:AA02870|3end_conserved_hypothetical_protein_2015552..2015702_POSITIVE