Origin IGS: agattttccttattgtgtatttaaaagttgacggagattgtattttaaattttacattctgtaaaacaaagatcgtgatataagccgcaaaatcttaattttaggtgaaatttatggggcggattgagtatagtaaaaattttcattccgctgtaaggggttccgccatgacattactcaattactttcaggatttagacacattacacatcaacaccgcaacacgcctattttattccccatgccaatgaccaaagtgcg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 cgcactttggtcattggcatggggaataaaataggcgtgttgcggtgttgatgtgtaatgtgtctaaatcctgaaagtaattgagtaatgtcatggcggaaccccttacagcggaatgaaaatttttactatactcaatccgccccataaatttcacctaaaattaagattttgcggcttatatcacgatctttgttttacagaatgtaaaatttaaaatacaatctccgtcaacttttaaatacacaataaggaaaatct Mask Tandem Repeat Region ================================================ agattttccttattgtgtatttaaaagttgacggagattgtattttaaattttacattctgtaaaacaaagatcgtgatataagccgcaaaatcttaattttaggtgaaatttatggggcggattgagtatagtaaaaattttcattccgctgtaaggggttccgccatgacattactcaattactttcaggatttagacacattacacatcaacaccgcaacacgcctattttattccccatgccaatgaccaaagtgcg Find is-nt database================================================ Query_seq: AA01704:AA01705|AA01704:AA01705:tyrosine-specific transport protein:beta-galactosidase:->->:1157522..1157782 261 agattttccttattgtgtatttaaaagttgacggagattgtattttaaattttacattctgtaaaacaaagatcgtgatataagccgcaaaatcttaattttaggtgaaatttatggggcggattgagtatagtaaaaattttcattccgctgtaaggggttccgccatgacattactcaattactttcaggatttagacacattacacatcaacaccgcaacacgcctattttattccccatgccaatgaccaaagtgcg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01704:AA01705|AA01704:AA01705:tyrosine-specific transport protein:beta-galactosidase:->->:1157522..1157782 261 agattttccttattgtgtatttaaaagttgacggagattgtattttaaattttacattctgtaaaacaaagatcgtgatataagccgcaaaatcttaattttaggtgaaatttatggggcggattgagtatagtaaaaattttcattccgctgtaaggggttccgccatgacattactcaattactttcaggatttagacacattacacatcaacaccgcaacacgcctattttattccccatgccaatgaccaaagtgcg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01704:AA01705|AA01704:AA01705:tyrosine-specific transport protein:beta-galactosidase:->->:1157522..1157782 261 agattttccttattgtgtatttaaaagttgacggagattgtattttaaattttacattctgtaaaacaaagatcgtgatataagccgcaaaatcttaattttaggtgaaatttatggggcggattgagtatagtaaaaattttcattccgctgtaaggggttccgccatgacattactcaattactttcaggatttagacacattacacatcaacaccgcaacacgcctattttattccccatgccaatgaccaaagtgcg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agattttccttattgtgtatttaaaagttgacggagattgtattttaaattttacattctgtaaaacaaagatcgtgatataagccgcaaaatcttaattttaggtgaaatttatggggcggattgagtatagtaaaaattttcattccgctgtaaggggttccgccatgacattactcaattactttcaggatttagacacattacacatcaacaccgcaacacgcctattttattccccatgccaatgaccaaagtgcg Predict ORF larger than 30AA ================================================ Protein_Len: 48 Strand: + Start: 105 End: 248 ........................................................................................................ M K F M G R I E Y S K N F H S A V R G S A M T L L N Y F Q D L D T L H I N T A T R L F Y S P C Q ............. Protein_Len: 33 Strand: - Start: 48 End: 146 ............................................... I K C E T F C L D H Y L G C F R L K L H F K H P P N L I T F I K M ................................................................................................................... agattttccttattgtgtatttaaaagttgacggagattgtattttaaattttacattctgtaaaacaaagatcgtgatataagccgcaaaatcttaattttaggtgaaatttatggggcggattgagtatagtaaaaattttcattccgctgtaaggggttccgccatgacattactcaattactttcaggatttagacacattacacatcaacaccgcaacacgcctattttattccccatgccaatgaccaaagtgcg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 84 Start: 37 End: 66 ....................................TTTTACAGAATGTAAAATTTAAAATACAAT................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agattttccttattgtgtatttaaaagttgacggagattgtattttaaattttacattctgtaaaacaaagatcgtgatataagccgcaaaatcttaattttaggtgaaatttatggggcggattgagtatagtaaaaattttcattccgctgtaaggggttccgccatgacattactcaattactttcaggatttagacacattacacatcaacaccgcaacacgcctattttattccccatgccaatgaccaaagtgcg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01704:AA01705|AA01704:AA01705:tyrosine-specific transport protein:beta-galactosidase:->->:1157522..1157782 261 agattttccttattgtgtatttaaaagttgacggagattgtattttaaattttacattctgtaaaacaaagatcgtgatataagccgcaaaatcttaattttaggtgaaatttatggggcggattgagtatagtaaaaattttcattccgctgtaaggggttccgccatgacattactcaattactttcaggatttagacacattacacatcaacaccgcaacacgcctattttattccccatgccaatgaccaaagtgcg Intra-Species Hit: Count: 1 Min: 1 Max: 261 Len: 261 Subject: aact_AA01704_AA01705|tyrosine-specific transport protein:beta-galactosidase|POSITIVE:POSITIVE|[1157522, 1157782]|261 HSP 1 e-value: 1.0E-146 bit: 517.0 Len: 261 Query Start:1 Query End:261 Subject Strand: POSITIVE Subject Start: 1 Subject End: 261 agattttccttattgtgtatttaaaagttgacggagattgtattttaaattttacattctgtaaaacaaagatcgtgatataagccgcaaaatcttaattttaggtgaaatttatggggcggattgagtatagtaaaaattttcattccgctgtaaggggttccgccatgacattactcaattactttcaggatttagacacattacacatcaacaccgcaacacgcctattttattccccatgccaatgaccaaagtgcg agattttccttattgtgtatttaaaagttgacggagattgtattttaaattttacattctgtaaaacaaagatcgtgatataagccgcaaaatcttaattttaggtgaaatttatggggcggattgagtatagtaaaaattttcattccgctgtaaggggttccgccatgacattactcaattactttcaggatttagacacattacacatcaacaccgcaacacgcctattttattccccatgccaatgaccaaagtgcg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAUUUUCCUUAUUGUGUAUUUAAAAGUUGACGGAGAUUGUAUUUUAAAUUUUACAUUCUGUAAAACAAAGAUCGUGAUAUAAGCCGCAAAAUCUUAAUUUUAGGUGAAAUUUAUGGGGCGGAUUGAGUAUAGUAAAAAUUUUCAUUCCGCUGUAAGGGGUUCCGCCAUGACAUUACUCAAUUACUUUCAGGAUUUAGACACAUUACACAUCAACACCGCAACACGCCUAUUUUAUUCCCCAUGCCAAUGACCAAAGUGCG ..(((((..((((((.((((......((((((((...(((((((.....((((((.....))))))..........))))))).)))..(((((((.......((((.(((((.((.((((((.((((.............)))).)))))).))..))))).)))).(((......))).........)))))))..............)))))...((.....))..............))))))))))..)))))... (-46.38) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGCACUUUGGUCAUUGGCAUGGGGAAUAAAAUAGGCGUGUUGCGGUGUUGAUGUGUAAUGUGUCUAAAUCCUGAAAGUAAUUGAGUAAUGUCAUGGCGGAACCCCUUACAGCGGAAUGAAAAUUUUUACUAUACUCAAUCCGCCCCAUAAAUUUCACCUAAAAUUAAGAUUUUGCGGCUUAUAUCACGAUCUUUGUUUUACAGAAUGUAAAAUUUAAAAUACAAUCUCCGUCAACUUUUAAAUACACAAUAAGGAAAAUCU .((((.(((..(((..((((.(.((..............)).).))))..)))..))).)))).....((((....(((.(((((....((..(((((((..........(((((.(((...............))).)))))....(((((((.((.((((((.((((((.((..........)).)))))).))))))......)).)))))))..........))))))))).))))).)))......))))...... (-48.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 337 173 141 63 25 aact:AA02179|5end_anthanilate_phosphoribosyltransferase_1489367..1489597_POSITIVE 330 137 93 140 99 aact:AA02603|5end_possible_thioredoxin_trxA_homolog_1814635..1814865_POSITIVE 321 260 218 48 1 aact:AA01732|5end_multidrug_resistance_protein_A_1170677..1170907_POSITIVE 319 256 215 192 155 aact:AA01981|5end_4-alpha-glucanotransferase_1337202..1337432_POSITIVE 319 256 215 45 8 aact:AA01978|5end_hypothetical_protein_1337055..1337285_POSITIVE 315 129 84 212 172 aact:AA02934|5end_hypothetical_protein_2054242..2054472_POSITIVE 313 189 141 83 25 aact:AA02045|5end_PTS_system,_galactitol-specific_IIB_component_1391956..1392186_POSITIVE 308 170 146 176 148 aact:AA02736|5end_conserved_hypothetical_protein_1917350..1917580_POSITIVE 307 245 208 225 174 aact:AA01408|5end_conserved_hypothetical_protein_950191..950421_POSITIVE 307 170 121 162 111 aact:AA00408|5end_SUN_protein_(FMU_protein)_272256..272486_POSITIVE 306 190 141 72 16 aact:AA01411|5end_stationary-phase_survival_protein_951000..951230_POSITIVE 306 240 197 79 38 aact:AA00603|5end_undecaprenyl_pyrophosphate_synthetase_(UPPS)_410283..410513_POSITIVE 302 126 86 150 117 aact:AA00249|5end_cysteinyl-tRNA_synthetase_(cysteine--tRNA_ligase)_173966..174196_POSITIVE 302 126 86 52 19 aact:AA00248|5end_hypothetical_protein_173868..174098_POSITIVE 300 260 211 139 73 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 299 228 188 195 160 aact:AA01933|5end_hypothetical_protein_1306060..1306290_POSITIVE 292 171 148 90 69 aact:AA01434|5end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_961631..961861_POSITIVE 289 260 213 176 131 aact:AA00735|5end_citrate_lyase,_beta_chain_502674..502904_POSITIVE 287 248 208 145 105 aact:AA00938|5end_L-xylulokinase_647556..647786_POSITIVE 285 167 143 216 194 aact:AA01798|5end_hypothetical_protein_1212070..1212300_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 337 173 141 48 10 aact:AA02177|3end_conserved_hypothetical_protein_1489432..1489582_POSITIVE 319 256 215 42 5 aact:AA01976|3end_colicin_V_production_protein_1337132..1337282_POSITIVE 313 189 141 61 3 aact:AA02044|3end_phosphotransferase_system_enzyme_II,_galactitol_specific,_protein_A_1392014..1392164_POSITIVE 307 170 121 135 84 aact:AA00407|3end_trk_system_potassium_uptake_protein_272309..272459_POSITIVE 302 126 86 50 17 aact:AA00247|3end_peptidyl-prolyl_cis-trans_isomerase_B_173946..174096_POSITIVE 299 228 188 147 112 aact:AA01932|3end_tRNA_pseudouridine_synthase_B_1306092..1306242_POSITIVE 292 171 148 145 124 aact:AA01432|3end_lysophospholipase_L2_961766..961916_POSITIVE 281 247 202 151 100 aact:AA02195|3end_oxaloacetate_decarboxylase_alpha_chain_1496374..1496524_POSITIVE 280 229 189 130 78 aact:AA01956|3end_3-deoxy-manno-octulosonate_cytidylyltransferase_1323286..1323436_POSITIVE 280 159 111 71 11 aact:AA01807|3end_electron_transport_complex_protein_1217363..1217513_POSITIVE 279 260 223 142 101 aact:AA00036|3end_transposase_29254..29404_POSITIVE 277 258 215 125 78 aact:AA02543|3end_conserved_hypothetical_protein_1773434..1773584_POSITIVE 271 181 148 135 101 aact:AA01254|3end_conserved_hypothetical_protein_(possible_proline_dipeptidase)_852702..852852_POSITIVE 268 149 101 129 78 aact:AA00430|3end_hypothetical_protein_293190..293340_POSITIVE 266 124 86 151 120 aact:AA00248|3end_hypothetical_protein_174049..174199_POSITIVE 261 248 202 131 86 aact:AA02602|3end_conserved_hypothetical_protein_1814302..1814452_POSITIVE 261 260 214 138 98 aact:AA00480|3end_mannose-specific_phosphotransferase_system_IID_335199..335349_POSITIVE 260 248 207 151 102 aact:AA02046|3end_PTS_system_galactitol-specific_enzyme_IIC_1393687..1393837_POSITIVE 260 153 117 80 43 aact:AA01340|3end_possible_lipoprotein;_possible_HlyD_family_secretion_domain_protein;_possible_membrane_fusion_protein_907019..907169_POSITIVE 257 260 217 145 97 aact:AA02089|3end_glycogen_operon_protein_1423212..1423362_POSITIVE