Origin IGS: taatcctgtaggtgggcttgtcccaccttttttgtctttcgatacgataaatccctcccgcgtacataaagtgcggtcacaaaaaattctgttttttgaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttacaaatgttttttgttagctcagaaaaaagagaatgattatattttacgcctgaaacttttcagctatactgacacaatttttttgactcgagaaaggtaacttttatgaaatttaaattaagtttaatttccaccgcactttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaagtgcggtggaaattaaacttaatttaaatttcataaaagttacctttctcgagtcaaaaaaattgtgtcagtatagctgaaaagtttcaggcgtaaaatataatcattctcttttttctgagctaacaaaaaacatttgtaaaaatattcataaatcactgaagtgatttattcaccccttcggggccgttggcatagccaacgttcaaaaaacagaattttttgtgaccgcactttatgtacgcgggagggatttatcgtatcgaaagacaaaaaaggtgggacaagcccacctacaggatta Mask Tandem Repeat Region ================================================ taatcctgtaggtgggcttgtcccaccttttttgtctttcgatacgataaatccctcccgcgtacataaagtgcggtcacaaaaaattctgttttttgaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttacaaatgttttttgttagctcagaaaaaagagaatgattatattttacgcctgaaacttttcagctatactgacacaatttttttgactcgagaaaggtaacttttatgaaatttaaattaagtttaatttccaccgcactttta Find is-nt database================================================ Query_seq: AA01675:AA01678|AA01675:AA01678:probable multidrug resistance protein; multidrug-efflux transporter:possible transcriptional regulator:->->:1133650..1133958 309 taatcctgtaggtgggcttgtcccaccttttttgtctttcgatacgataaatccctcccgcgtacataaagtgcggtcacaaaaaattctgttttttgaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttacaaatgttttttgttagctcagaaaaaagagaatgattatattttacgcctgaaacttttcagctatactgacacaatttttttgactcgagaaaggtaacttttatgaaatttaaattaagtttaatttccaccgcactttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01675:AA01678|AA01675:AA01678:probable multidrug resistance protein; multidrug-efflux transporter:possible transcriptional regulator:->->:1133650..1133958 309 taatcctgtaggtgggcttgtcccaccttttttgtctttcgatacgataaatccctcccgcgtacataaagtgcggtcacaaaaaattctgttttttgaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttacaaatgttttttgttagctcagaaaaaagagaatgattatattttacgcctgaaacttttcagctatactgacacaatttttttgactcgagaaaggtaacttttatgaaatttaaattaagtttaatttccaccgcactttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01675:AA01678|AA01675:AA01678:probable multidrug resistance protein; multidrug-efflux transporter:possible transcriptional regulator:->->:1133650..1133958 309 taatcctgtaggtgggcttgtcccaccttttttgtctttcgatacgataaatccctcccgcgtacataaagtgcggtcacaaaaaattctgttttttgaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttacaaatgttttttgttagctcagaaaaaagagaatgattatattttacgcctgaaacttttcagctatactgacacaatttttttgactcgagaaaggtaacttttatgaaatttaaattaagtttaatttccaccgcactttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatcctgtaggtgggcttgtcccaccttttttgtctttcgatacgataaatccctcccgcgtacataaagtgcggtcacaaaaaattctgttttttgaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttacaaatgttttttgttagctcagaaaaaagagaatgattatattttacgcctgaaacttttcagctatactgacacaatttttttgactcgagaaaggtaacttttatgaaatttaaattaagtttaatttccaccgcactttta Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 32 End: 133 ............................... M S F D T I N P S R V H K V R S Q K I L F F E R W L C Q R P R R G E ................................................................................................................................................................................ Protein_Len: 32 Strand: + Start: 51 End: 146 .................................................. M P P A Y I K C G H K K F C F L N V G Y A N G P E G V N K S L Q ................................................................................................................................................................... Protein_Len: 42 Strand: + Start: 137 End: 262 ........................................................................................................................................ M T S V I Y E Y F Y K C F L L A Q K K E N D Y I L R L K L F S Y T D T I F L T R E R ............................................... Protein_Len: 44 Strand: - Start: 3 End: 134 .. D Q L H A Q G V K K T K R Y S L D R G R V Y L A T V F F E T K Q V N A I G V A G F P H M ............................................................................................................................................................................... Protein_Len: 50 Strand: - Start: 1 End: 150 L G T P P S T G G K K D K S V I F G E R T C L T R D C F I R N K S R Q S H W R G R L P S Y I V E T M ............................................................................................................................................................... taatcctgtaggtgggcttgtcccaccttttttgtctttcgatacgataaatccctcccgcgtacataaagtgcggtcacaaaaaattctgttttttgaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttacaaatgttttttgttagctcagaaaaaagagaatgattatattttacgcctgaaacttttcagctatactgacacaatttttttgactcgagaaaggtaacttttatgaaatttaaattaagtttaatttccaccgcactttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatcctgtaggtgggcttgtcccaccttttttgtctttcgatacgataaatccctcccgcgtacataaagtgcggtcacaaaaaattctgttttttgaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttacaaatgttttttgttagctcagaaaaaagagaatgattatattttacgcctgaaacttttcagctatactgacacaatttttttgactcgagaaaggtaacttttatgaaatttaaattaagtttaatttccaccgcactttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01675:AA01678|AA01675:AA01678:probable multidrug resistance protein; multidrug-efflux transporter:possible transcriptional regulator:->->:1133650..1133958 309 taatcctgtaggtgggcttgtcccaccttttttgtctttcgatacgataaatccctcccgcgtacataaagtgcggtcacaaaaaattctgttttttgaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttacaaatgttttttgttagctcagaaaaaagagaatgattatattttacgcctgaaacttttcagctatactgacacaatttttttgactcgagaaaggtaacttttatgaaatttaaattaagtttaatttccaccgcactttta Intra-Species Hit: Count: 2 Min: 1 Max: 309 Len: 309 Subject: aact_AA01675_AA01678|probable multidrug resistance protein; multidrug-efflux transporter:possible transcriptional regulator|POSITIVE:POSITIVE|[1133650,1133958]|309 HSP 1 e-value: 1.0E-162 bit: 571.0 Len: 309 Query Start:1 Query End:309 Subject Strand: POSITIVE Subject Start: 1 Subject End: 309 taatcctgtaggtgggcttgtcccaccttttttgtctttcgatacgataaatccctcccgcgtacataaagtgcggtcacaaaaaattctgttttttgaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttacaaatgttttttgttagctcagaaaaaagagaatgattatattttacgcctgaaacttttcagctatactgacacaannnnnnngactcgagaaaggtaacttttatgaaatttaaattaagtttaatttccaccgcactttta taatcctgtaggtgggcttgtcccaccttttttgtctttcgatacgataaatccctcccgcgtacataaagtgcggtcacaaaaaattctgttttttgaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttacaaatgttttttgttagctcagaaaaaagagaatgattatattttacgcctgaaacttttcagctatactgacacaatttttttgactcgagaaaggtaacttttatgaaatttaaattaagtttaatttccaccgcactttta Subject: aact_AA00987_AA00988|tetracenomycin polyketide synthesis O-methyltransferase:conserved hypothetical protein|POSITIVE:POSITIVE|[690647,691037]|391 HSP 1 e-value: 3.0E-43 bit: 176.0 Len: 109 Query Start:68 Query End:176 Subject Strand: NEGATIVE Subject Start: 215 Subject End: 323 ...................................................................aaagtgcggtcacaaaaaattctgttttttgaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttacaaatgttttt..................................................................................................................................... ...................................................................aaagtgcggtcacaaaaaattccgttttttgaacgttggttatgccaagggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttccaaacgttttt..................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCCUGUAGGUGGGCUUGUCCCACCUUUUUUGUCUUUCGAUACGAUAAAUCCCUCCCGCGUACAUAAAGUGCGGUCACAAAAAAUUCUGUUUUUUGAACGUUGGCUAUGCCAACGGCCCCGAAGGGGUGAAUAAAUCACUUCAGUGAUUUAUGAAUAUUUUUACAAAUGUUUUUUGUUAGCUCAGAAAAAAGAGAAUGAUUAUAUUUUACGCCUGAAACUUUUCAGCUAUACUGACACAAUUUUUUUGACUCGAGAAAGGUAACUUUUAUGAAAUUUAAAUUAAGUUUAAUUUCCACCGCACUUUUA .........(((((((.....)))))))..((((((.........))))))................((((((((((..(((((((.(((.((((((((.(((((((...)))))))..(((....)))...(((((((((....)))))))))..........(((((.....)))))....)))))))).))).................((.((((....))))))..............)))))))..(((.(((((....))))).))).......................)))))))))).. (-72.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAGUGCGGUGGAAAUUAAACUUAAUUUAAAUUUCAUAAAAGUUACCUUUCUCGAGUCAAAAAAAUUGUGUCAGUAUAGCUGAAAAGUUUCAGGCGUAAAAUAUAAUCAUUCUCUUUUUUCUGAGCUAACAAAAAACAUUUGUAAAAAUAUUCAUAAAUCACUGAAGUGAUUUAUUCACCCCUUCGGGGCCGUUGGCAUAGCCAACGUUCAAAAAACAGAAUUUUUUGUGACCGCACUUUAUGUACGCGGGAGGGAUUUAUCGUAUCGAAAGACAAAAAAGGUGGGACAAGCCCACCUACAGGAUUA .....((((((((((.....(((((................)))))..((((((((.((((...(((((...((((.....))))..)))))...(((...............(((........)))...((((((((..(((((..........(((((((((....)))))))))....(((....))).(((((((...)))))))........))))).))))))))...)))......)).)).)))))))).))))))))))(....).......(((((((.....)))))))......... (-75.49) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 780 139 91 231 183 aact:AA00988|5end_conserved_hypothetical_protein_690898..691128_POSITIVE 689 136 91 132 86 aact:AA01098|5end_bis(5'-nucleosyl)-tetraphosphatase_757699..757929_POSITIVE 654 132 91 225 181 aact:AA00200|5end_thymidylate_synthase_138892..139122_POSITIVE 645 136 94 195 152 aact:AA02108|5end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1433393..1433623_POSITIVE 636 136 95 43 1 aact:AA02106|5end_hypothetical_protein_1433241..1433471_POSITIVE 608 150 101 201 152 aact:AA02666|5end_DNA_helicase_II_1854410..1854640_POSITIVE 574 134 92 193 151 aact:AA01090|5end_leucyl-tRNA_synthetase,_leucyl-tRNA_ligase_753548..753778_POSITIVE 569 139 91 164 116 aact:AA01633|5end_probable_ferredoxin-like_protein_1104008..1104238_POSITIVE 512 134 98 37 1 aact:AA01089|5end_hypothetical_protein_753392..753622_POSITIVE 434 94 54 231 185 aact:AA00505|5end_hypothetical_protein_351561..351791_POSITIVE 416 57 11 162 108 aact:AA01635|5end_ribonucleoside_diphosphate_reductase,_beta_chain_1104615..1104845_POSITIVE 383 100 56 230 185 aact:AA02168|5end_possible_protease;_heat_shock_protein_1483529..1483759_POSITIVE 377 80 31 161 101 aact:AA01867|5end_thiamine_transport_ATP-binding_protein_1254853..1255083_POSITIVE 374 60 11 212 171 aact:AA02949|5end_hypothetical_protein_2061944..2062174_POSITIVE 362 131 98 164 116 aact:AA00413|5end_conserved_hypothetical_protein_280142..280372_POSITIVE 361 130 82 145 106 aact:AA00048|5end_conserved_hypothetical_protein_38853..39083_POSITIVE 347 79 34 218 174 aact:AA02426|5end_uridylyltransferase_1671663..1671893_POSITIVE 345 144 102 208 169 aact:AA00119|5end_ATP-dependent_Clp_protease_proteolytic_subunit_(endopeptidase_Clp)_88387..88617_POSITIVE 345 144 102 67 28 aact:AA00117|5end_hypothetical_protein_88246..88476_POSITIVE 330 148 110 167 129 aact:AA02132|5end_dihydroorotate_dehydrogenase_1455089..1455319_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 608 150 101 62 13 aact:AA02663|3end_DNA_ligase_1854351..1854501_POSITIVE 602 120 71 115 66 aact:AA02167|3end_hypothetical_protein_1483474..1483624_POSITIVE 592 136 100 38 1 aact:AA01097|3end_conserved_hypothetical_protein_757685..757835_POSITIVE 589 140 91 61 16 aact:AA01342|3end_conserved_hypotheticl_protein_(possible_ribosomal_RNA_adenine_dimethylase)_911085..911235_POSITIVE 574 134 92 48 6 aact:AA01088|3end_rare_lipoprotein_B_precursor_753483..753633_POSITIVE 416 57 11 137 83 aact:AA01634|3end_hypothetical_protein_1104670..1104820_POSITIVE 378 120 71 148 98 aact:AA01089|3end_hypothetical_protein_753597..753747_POSITIVE 374 60 11 62 21 aact:AA02947|3end_riboflavin_biosynthesis_protein_2061874..2062024_POSITIVE 347 79 34 136 92 aact:AA02425|3end_glutamate-1-semialdehyde_aminotransferase_1671661..1671811_POSITIVE 337 120 71 140 91 aact:AA00117|3end_hypothetical_protein_88418..88568_POSITIVE 330 148 110 150 112 aact:AA02131|3end_conserved_hypothetical_protein_(possible_TrpH_protein)_1455152..1455302_POSITIVE 327 40 10 97 64 aact:AA03008|3end_GTP-binding_protein_era_homolog_(GTPase)_2101072..2101222_POSITIVE 325 58 11 135 82 aact:AA00550|3end_ferredoxin-type_protein_377201..377351_POSITIVE 324 129 91 85 43 aact:AA02468|3end_conserved_hypothetical_protein_1706797..1706947_POSITIVE 318 131 101 131 105 aact:AA03010|3end_thiophene_and_furan_oxidation_protein_2102534..2102684_POSITIVE 315 78 45 127 98 aact:AA01297|3end_competence_protein_E_precursor_880611..880761_POSITIVE 315 110 61 91 30 aact:AA00896|3end_prolyl-tRNA_synthetase_(proline-tRNA_ligase)_623206..623356_POSITIVE 311 46 10 76 39 aact:AA02013|3end_carbonic_anhydrase_1370374..1370524_POSITIVE 311 131 91 59 20 aact:AA01269|3end_hypothetical_protein_863413..863563_POSITIVE 311 79 32 131 88 aact:AA01160|3end_ADP_compounds_hydrolase_794190..794340_POSITIVE