Origin IGS: aataatctctttgaaaaaattgatggctgattctaatgcaatatttatttcgatacaacatatcgggtgaaagaaccttatgttgataaaggtaaagtgttaactatacttgacacttttaagctaaagactatacttaggcgaaacaaataacgacaattaattca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaattaattgtcgttatttgtttcgcctaagtatagtctttagcttaaaagtgtcaagtatagttaacactttacctttatcaacataaggttctttcacccgatatgttgtatcgaaataaatattgcattagaatcagccatcaattttttcaaagagattatt Mask Tandem Repeat Region ================================================ aataatctctttgaaaaaattgatggctgattctaatgcaatatttatttcgatacaacatatcgggtgaaagaaccttatgttgataaaggtaaagtgttaactatacttgacacttttaagctaaagactatacttaggcgaaacaaataacgacaattaattca Find is-nt database================================================ Query_seq: AA01670:AA01671|AA01670:AA01671:conserved hypothetical protein:conserved hypothetical protein:->->:1130822..1130988 167 aataatctctttgaaaaaattgatggctgattctaatgcaatatttatttcgatacaacatatcgggtgaaagaaccttatgttgataaaggtaaagtgttaactatacttgacacttttaagctaaagactatacttaggcgaaacaaataacgacaattaattca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01670:AA01671|AA01670:AA01671:conserved hypothetical protein:conserved hypothetical protein:->->:1130822..1130988 167 aataatctctttgaaaaaattgatggctgattctaatgcaatatttatttcgatacaacatatcgggtgaaagaaccttatgttgataaaggtaaagtgttaactatacttgacacttttaagctaaagactatacttaggcgaaacaaataacgacaattaattca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01670:AA01671|AA01670:AA01671:conserved hypothetical protein:conserved hypothetical protein:->->:1130822..1130988 167 aataatctctttgaaaaaattgatggctgattctaatgcaatatttatttcgatacaacatatcgggtgaaagaaccttatgttgataaaggtaaagtgttaactatacttgacacttttaagctaaagactatacttaggcgaaacaaataacgacaattaattca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aataatctctttgaaaaaattgatggctgattctaatgcaatatttatttcgatacaacatatcgggtgaaagaaccttatgttgataaaggtaaagtgttaactatacttgacacttttaagctaaagactatacttaggcgaaacaaataacgacaattaattca Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 43 End: 150 .......................................... M Y F D T T Y R V K E P Y V D K G K V L T I L D T F K L K T I L R R N K ................. aataatctctttgaaaaaattgatggctgattctaatgcaatatttatttcgatacaacatatcgggtgaaagaaccttatgttgataaaggtaaagtgttaactatacttgacacttttaagctaaagactatacttaggcgaaacaaataacgacaattaattca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aataatctctttgaaaaaattgatggctgattctaatgcaatatttatttcgatacaacatatcgggtgaaagaaccttatgttgataaaggtaaagtgttaactatacttgacacttttaagctaaagactatacttaggcgaaacaaataacgacaattaattca Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 53 HP_score: -2.9 Tail_Score: -4.13767 Start: 97 End: 116 Full_Region: GTTGATAAAGGTAAA GTGTTAA CTATAC TTGACAC TTTTAAGCTAAAGAC ................................................................................................GTGTTAACTATACTTGACAC................................................... Find igs database================================================ Query_seq: AA01670:AA01671|AA01670:AA01671:conserved hypothetical protein:conserved hypothetical protein:->->:1130822..1130988 167 aataatctctttgaaaaaattgatggctgattctaatgcaatatttatttcgatacaacatatcgggtgaaagaaccttatgttgataaaggtaaagtgttaactatacttgacacttttaagctaaagactatacttaggcgaaacaaataacgacaattaattca Intra-Species Hit: Count: 1 Min: 1 Max: 167 Len: 167 Subject: aact_AA01670_AA01671|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1130822,1130988]|167 HSP 1 e-value: 4.0E-90 bit: 331.0 Len: 167 Query Start:1 Query End:167 Subject Strand: POSITIVE Subject Start: 1 Subject End: 167 aataatctctttgaaaaaattgatggctgattctaatgcaatatttatttcgatacaacatatcgggtgaaagaaccttatgttgataaaggtaaagtgttaactatacttgacacttttaagctaaagactatacttaggcgaaacaaataacgacaattaattca aataatctctttgaaaaaattgatggctgattctaatgcaatatttatttcgatacaacatatcgggtgaaagaaccttatgttgataaaggtaaagtgttaactatacttgacacttttaagctaaagactatacttaggcgaaacaaataacgacaattaattca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAAUCUCUUUGAAAAAAUUGAUGGCUGAUUCUAAUGCAAUAUUUAUUUCGAUACAACAUAUCGGGUGAAAGAACCUUAUGUUGAUAAAGGUAAAGUGUUAACUAUACUUGACACUUUUAAGCUAAAGACUAUACUUAGGCGAAACAAAUAACGACAAUUAAUUCA ...........((((..(((((...((..........))..(((((.(((((...(((((((..((((......))))))))))).....(((((((((((((......))))))))))...))).....(((....))).))))).)))))....)))))..)))) (-31.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAUUAAUUGUCGUUAUUUGUUUCGCCUAAGUAUAGUCUUUAGCUUAAAAGUGUCAAGUAUAGUUAACACUUUACCUUUAUCAACAUAAGGUUCUUUCACCCGAUAUGUUGUAUCGAAAUAAAUAUUGCAUUAGAAUCAGCCAUCAAUUUUUUCAAAGAGAUUAUU ((((..(((((....(((((((((((......................(((((((.((......)).)))))))........(((((((.(((......)))...)))))))...)))))))))))..((..........))...)))))..))))........... (-26.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 247 113 72 154 112 aact:AA01367|5end_tRNA_(Guanine-N(1)-)-methyltransferase_(M1G-methyltransferase)_923710..923940_POSITIVE 232 80 37 157 116 aact:AA01763|5end_conserved_hypothetical_protein_(possible_transcriptional_regulatory_protein)_1190643..1190873_POSITIVE 232 142 109 142 108 aact:AA00530|5end_hypothetical_protein_362373..362603_POSITIVE 229 99 54 192 137 aact:AA02932|5end_lytic_murein_transglycosylase_A_2052349..2052579_POSITIVE 228 108 64 92 41 aact:AA02196|5end_oxaloacetate_decarboxylase_beta_chain_1496308..1496538_POSITIVE 228 150 102 217 159 aact:AA00869|5end_tight_adherence_protein_V;_prepilin_peptidase_601255..601485_POSITIVE 221 103 63 57 13 aact:AA00864|5end_conserved_hypothetical_protein_598605..598835_POSITIVE 220 104 65 83 43 aact:AA02762|5end_ribonuclease_H_1933164..1933394_POSITIVE 220 143 101 213 164 aact:AA02171|5end_polysialic_acid_capsule_expression_protein_1485084..1485314_POSITIVE 218 158 123 158 113 aact:AA02906|5end_hypothetical_protein_2033304..2033534_POSITIVE 218 70 24 208 163 aact:AA02091|5end_hypothetical_protein_1424441..1424671_POSITIVE 217 156 111 231 187 aact:AA02913|5end_ribosomal_large_subunit_pseudouridine_synthase_2039491..2039721_POSITIVE 217 56 25 45 6 aact:AA00194|5end_oxygen-independent_coproporphyrinogen_III_oxidase_135545..135775_POSITIVE 217 148 104 158 107 aact:AA00033|5end_conserved_hypothetical_protein_25643..25873_POSITIVE 211 70 21 87 37 aact:AA01299|5end_competence_protein_C_880946..881176_POSITIVE 210 91 63 50 16 aact:AA00078|5end_hypothetical_protein_57468..57698_POSITIVE 208 99 62 149 105 aact:AA02380|5end_iron_chelatin_ABC_transporter,periplasmic-binding_protein_1628057..1628287_POSITIVE 208 99 62 46 2 aact:AA02379|5end_hypothetical_protein_1627954..1628184_POSITIVE 207 107 61 87 34 aact:AA02638|5end_conserved_hypothetical_protein_(possible_reductase)_1836469..1836699_POSITIVE 207 107 65 64 26 aact:AA00930|5end_pantothenate_kinase_640275..640505_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 247 113 72 85 43 aact:AA01366|3end_16S_rRNA_processing_protein_923721..923871_POSITIVE 234 82 47 140 97 aact:AA02409|3end_DNA_ligase_1655404..1655554_POSITIVE 232 142 109 59 25 aact:AA00529|3end_outer_membrane_protein,_Omp_18/16_362370..362520_POSITIVE 228 108 64 78 27 aact:AA02195|3end_oxaloacetate_decarboxylase_alpha_chain_1496374..1496524_POSITIVE 226 81 58 26 6 aact:AA01762|3end_hypothetical_protein_1190591..1190741_POSITIVE 221 103 63 50 6 aact:AA00863|3end_conserved_hypothetical_protein_598678..598828_POSITIVE 211 70 21 84 34 aact:AA01298|3end_competence_protein_D_881023..881173_POSITIVE 210 91 63 36 2 aact:AA00077|3end_conserved_hypothetical_protein_(possible_periplasmic_binding_transport_protein)_57534..57684_POSITIVE 208 99 62 135 91 aact:AA02379|3end_hypothetical_protein_1628123..1628273_POSITIVE 207 107 61 97 44 aact:AA02637|3end_conserved_hypothetical_protein_(possible_membrane_protein)_1836559..1836709_POSITIVE 205 99 58 88 52 aact:AA00371|3end_transcriptional_regulator_250154..250304_POSITIVE 201 141 104 148 101 aact:AA00031|3end_conserved_hypothetical_protein_25717..25867_POSITIVE 200 85 49 97 65 aact:AA01873|3end_mannose-6-phosphate_isomerase_1261940..1262090_POSITIVE 195 92 62 81 46 aact:AA02736|3end_conserved_hypothetical_protein_1918581..1918731_POSITIVE 194 95 64 138 108 aact:AA02431|3end_conserved_hypothetical_protein_1675886..1676036_POSITIVE 194 97 51 70 15 aact:AA01978|3end_hypothetical_protein_1337257..1337407_POSITIVE 193 68 21 118 61 aact:AA01031|3end_Na(+)-translocating_NADH-quinone_reductase_subunit_C_721077..721227_POSITIVE 193 106 65 60 22 aact:AA00982|3end_aldo-keto_reductase_687583..687733_POSITIVE 193 143 101 44 6 aact:AA00806|3end_fumarate_reductase_15_kD_hydrophobic_protein_552834..552984_POSITIVE 191 160 119 62 5 aact:AA01738|3end_enoyl-(acyl-carrier-protein)_reductase_1175564..1175714_POSITIVE