Origin IGS: agtggatccttaaataaaaaagtacggtcattatactaaaagttttcatgctacatagtcattatcttcattttattattgccttggagtttgctaaaatgcgccattattttcattgggagcagtaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttactgctcccaatgaaaataatggcgcattttagcaaactccaaggcaataataaaatgaagataatgactatgtagcatgaaaacttttagtataatgaccgtacttttttatttaaggatccact Mask Tandem Repeat Region ================================================ agtggatccttaaataaaaaagtacggtcattatactaaaagttttcatgctacatagtcattatcttcattttattattgccttggagtttgctaaaatgcgccattattttcattgggagcagtaa Find is-nt database================================================ Query_seq: AA01662:AA01663|AA01662:AA01663:molybdopterin biosynthesis protein:GTP cyclohydrolase I:->->:1127483..1127610 128 agtggatccttaaataaaaaagtacggtcattatactaaaagttttcatgctacatagtcattatcttcattttattattgccttggagtttgctaaaatgcgccattattttcattgggagcagtaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01662:AA01663|AA01662:AA01663:molybdopterin biosynthesis protein:GTP cyclohydrolase I:->->:1127483..1127610 128 agtggatccttaaataaaaaagtacggtcattatactaaaagttttcatgctacatagtcattatcttcattttattattgccttggagtttgctaaaatgcgccattattttcattgggagcagtaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01662:AA01663|AA01662:AA01663:molybdopterin biosynthesis protein:GTP cyclohydrolase I:->->:1127483..1127610 128 agtggatccttaaataaaaaagtacggtcattatactaaaagttttcatgctacatagtcattatcttcattttattattgccttggagtttgctaaaatgcgccattattttcattgggagcagtaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agtggatccttaaataaaaaagtacggtcattatactaaaagttttcatgctacatagtcattatcttcattttattattgccttggagtttgctaaaatgcgccattattttcattgggagcagtaa Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 30 End: 125 ............................. M I L K V F M L H S H Y L H F I I A L E F A K M R H Y F H W E Q * ... agtggatccttaaataaaaaagtacggtcattatactaaaagttttcatgctacatagtcattatcttcattttattattgccttggagtttgctaaaatgcgccattattttcattgggagcagtaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 71 End: 100 ......................................................................TTTTATTATTGCCTTGGAGTTTGCTAAAAT............................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agtggatccttaaataaaaaagtacggtcattatactaaaagttttcatgctacatagtcattatcttcattttattattgccttggagtttgctaaaatgcgccattattttcattgggagcagtaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01662:AA01663|AA01662:AA01663:molybdopterin biosynthesis protein:GTP cyclohydrolase I:->->:1127483..1127610 128 agtggatccttaaataaaaaagtacggtcattatactaaaagttttcatgctacatagtcattatcttcattttattattgccttggagtttgctaaaatgcgccattattttcattgggagcagtaa Intra-Species Hit: Count: 1 Min: 1 Max: 128 Len: 128 Subject: aact_AA01662_AA01663|molybdopterin biosynthesis protein:GTP cyclohydrolase I|POSITIVE:POSITIVE|[1127483,1127610]|128 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:1 Query End:128 Subject Strand: POSITIVE Subject Start: 1 Subject End: 128 agtggatccttaaataaaaaagtacggtcattatactaaaagttttcatgctacatagtcattatcttcattttattattgccttggagtttgctaaaatgcgccattattttcattgggagcagtaa agtggatccttaaataaaaaagtacggtcattatactaaaagttttcatgctacatagtcattatcttcattttattattgccttggagtttgctaaaatgcgccattattttcattgggagcagtaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUGGAUCCUUAAAUAAAAAAGUACGGUCAUUAUACUAAAAGUUUUCAUGCUACAUAGUCAUUAUCUUCAUUUUAUUAUUGCCUUGGAGUUUGCUAAAAUGCGCCAUUAUUUUCAUUGGGAGCAGUAA ((..(((((...(((((.(((((..((.......((((..(((......)))...))))......))..))))).))))).....))).))..))....(((.(((..........)))..))).... (-17.82) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUACUGCUCCCAAUGAAAAUAAUGGCGCAUUUUAGCAAACUCCAAGGCAAUAAUAAAAUGAAGAUAAUGACUAUGUAGCAUGAAAACUUUUAGUAUAAUGACCGUACUUUUUUAUUUAAGGAUCCACU .......(((.((((((((..(((((.(((((((.......(....)......))))))).........((((...((........))..)))).....).))))...))))))))...)))...... (-13.52) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 307 116 79 201 161 aact:AA00424|5end_hypothetical_protein_287123..287353_POSITIVE 281 120 71 182 138 aact:AA01370|5end_rod_shape-determining_protein_925436..925666_POSITIVE 278 114 73 158 109 aact:AA00109|5end_conserved_hypothetical_protein_78582..78812_POSITIVE 258 118 79 180 137 aact:AA01792|5end_conserved_hypothetical_protein_1208058..1208288_POSITIVE 253 120 79 159 117 aact:AA00719|5end_hypothetical_protein_494627..494857_POSITIVE 252 105 75 60 32 aact:AA02651|5end_glycosyltransferase_1846440..1846670_POSITIVE 249 120 72 166 129 aact:AA00559|5end_conserved_hypothetical_protein_383167..383397_POSITIVE 247 104 67 123 87 aact:AA02329|5end_conserved_hypothetical_protein_1589659..1589889_POSITIVE 241 119 79 182 145 aact:AA02731|5end_outer_membrane_lipoprotein_carrier_precursor_1911498..1911728_POSITIVE 241 94 58 190 149 aact:AA02511|5end_transmembrane_protein_1742047..1742277_POSITIVE 241 96 54 125 89 aact:AA01013|5end_phosphomannomutase_708029..708259_POSITIVE 241 118 81 190 150 aact:AA00519|5end_O-acetyltransferase_357139..357369_POSITIVE 241 120 81 220 184 aact:AA00333|5end_1-phosphofructokinase;_tagatose-6-phosphate_kinase_225543..225773_POSITIVE 239 104 62 89 30 aact:AA01003|5end_conserved_hypothetical_protein_700120..700350_POSITIVE 238 106 75 130 94 aact:AA01295|5end_3-dehydroquinate_synthase_877272..877502_POSITIVE 237 119 79 208 175 aact:AA01810|5end_electron_transport_complex_protein_1219250..1219480_POSITIVE 236 119 80 64 20 aact:AA01519.1|5end_conserved_hypothetical_protein_1022466..1022696_POSITIVE 234 119 79 81 37 aact:AA02936|5end_lipoprotein_E_precursor;_outer_membrane_protein_P4_precursor;_acid_phosphatase_2055610..2055840_POSITIVE 231 108 66 76 31 aact:AA01363|5end_hypothetical_protein_922505..922735_POSITIVE 231 120 78 227 184 aact:AA00579|5end_probable_DNA_recombination_protein_395265..395495_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 306 120 78 82 30 aact:AA01873|3end_mannose-6-phosphate_isomerase_1261940..1262090_POSITIVE 278 114 73 144 95 aact:AA00108|3end_conserved_hypothetical_protein_78648..78798_POSITIVE 257 120 85 73 38 aact:AA02249|3end_thiamine_biosynthesis_protein_1529899..1530049_POSITIVE 249 120 72 147 110 aact:AA00558|3end_UDP-n-acetylenolpyruvoylglucosamine_reductase_383228..383378_POSITIVE 247 104 67 120 84 aact:AA02328|3end_anti-sigma_factor_B_antagonist_1589736..1589886_POSITIVE 241 96 54 90 54 aact:AA01012|3end_probable_carbon_storage_regulator_homolog_708074..708224_POSITIVE 239 106 79 131 102 aact:AA01878|3end_hydroxyacylglutathione_hydrolase_1265609..1265759_POSITIVE 238 106 75 145 109 aact:AA01294|3end_DNA_adenine_methylase_877367..877517_POSITIVE 236 119 80 57 13 aact:AA01519|3end_conserved_hypothetical_protein_1022539..1022689_POSITIVE 235 105 71 33 2 aact:AA01666|3end_penicillin-binding_protein_4;_D-alanyl-D-alanine_carboxypeptidase;_D-alanyl-D-alanine-endopeptidase_1129621..1129771_POSITIVE 233 36 2 131 91 aact:AA00151|3end_polynucleotide_phosphorylase_111071..111221_POSITIVE 231 120 78 83 40 aact:AA00578|3end_DNA_topoisomerase_395201..395351_POSITIVE 229 51 17 104 69 aact:AA01270|3end_hydrolase_(possible_2-haloalkanoic_acid_dehalogenase_I)_863897..864047_POSITIVE 228 120 71 148 98 aact:AA01222|3end_hypothetical_protein_834386..834536_POSITIVE 228 119 76 56 16 aact:AA00132|3end_conserved_hypothetical_protein_(possible_regulator_protein,_VirR_family)_95299..95449_POSITIVE 227 115 79 65 27 aact:AA01113|3end_transcriptional_regulator_(merR_family)_765249..765399_POSITIVE 227 54 17 62 30 aact:AA00659|3end_heat_shock_protein_453027..453177_POSITIVE 225 107 77 45 22 aact:AA02626|3end_conserved_hypothetical_protein_1829202..1829352_POSITIVE 224 104 79 99 68 aact:AA01345|3end_conserved_hypothetical_protein_(possible_membrane_protein)_913261..913411_POSITIVE 223 105 70 150 121 aact:AA01969|3end_hypothetical_protein_1332165..1332315_POSITIVE