Origin IGS: taaccaaaatagctcgaagcagatagtgcacgtcttccaacgcatacttctagtattcaggtaatgtatatggttgctagccgaaaaaacatttgaaattttaactgcacttttcatttatgatggttaagagcccacgcttggagtaacggaagcaccggcttagtgccgttgctatcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagatagcaacggcactaagccggtgcttccgttactccaagcgtgggctcttaaccatcataaatgaaaagtgcagttaaaatttcaaatgttttttcggctagcaaccatatacattacctgaatactagaagtatgcgttggaagacgtgcactatctgcttcgagctattttggtta Mask Tandem Repeat Region ================================================ taaccaaaatagctcgaagcagatagtgcacgtcttccaacgcatacttctagtattcaggtaatgtatatggttgctagccgaaaaaacatttgaaattttaactgcacttttcatttatgatggttaagagcccacgcttggagtaacggaagcaccggcttagtgccgttgctatcta Find is-nt database================================================ Query_seq: AA01660:AA01661|AA01660:AA01661:hypothetical protein:molybdopterin biosynthesis protein:->->:1125343..1125523 181 taaccaaaatagctcgaagcagatagtgcacgtcttccaacgcatacttctagtattcaggtaatgtatatggttgctagccgaaaaaacatttgaaattttaactgcacttttcatttatgatggttaagagcccacgcttggagtaacggaagcaccggcttagtgccgttgctatcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01660:AA01661|AA01660:AA01661:hypothetical protein:molybdopterin biosynthesis protein:->->:1125343..1125523 181 taaccaaaatagctcgaagcagatagtgcacgtcttccaacgcatacttctagtattcaggtaatgtatatggttgctagccgaaaaaacatttgaaattttaactgcacttttcatttatgatggttaagagcccacgcttggagtaacggaagcaccggcttagtgccgttgctatcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01660:AA01661|AA01660:AA01661:hypothetical protein:molybdopterin biosynthesis protein:->->:1125343..1125523 181 taaccaaaatagctcgaagcagatagtgcacgtcttccaacgcatacttctagtattcaggtaatgtatatggttgctagccgaaaaaacatttgaaattttaactgcacttttcatttatgatggttaagagcccacgcttggagtaacggaagcaccggcttagtgccgttgctatcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaccaaaatagctcgaagcagatagtgcacgtcttccaacgcatacttctagtattcaggtaatgtatatggttgctagccgaaaaaacatttgaaattttaactgcacttttcatttatgatggttaagagcccacgcttggagtaacggaagcaccggcttagtgccgttgctatcta Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: - Start: 1 End: 117 L W F L E F C I T C T K W R M S R T N L Y H I H N S A S F V N S I K V A S K M ................................................................ taaccaaaatagctcgaagcagatagtgcacgtcttccaacgcatacttctagtattcaggtaatgtatatggttgctagccgaaaaaacatttgaaattttaactgcacttttcatttatgatggttaagagcccacgcttggagtaacggaagcaccggcttagtgccgttgctatcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaccaaaatagctcgaagcagatagtgcacgtcttccaacgcatacttctagtattcaggtaatgtatatggttgctagccgaaaaaacatttgaaattttaactgcacttttcatttatgatggttaagagcccacgcttggagtaacggaagcaccggcttagtgccgttgctatcta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01660:AA01661|AA01660:AA01661:hypothetical protein:molybdopterin biosynthesis protein:->->:1125343..1125523 181 taaccaaaatagctcgaagcagatagtgcacgtcttccaacgcatacttctagtattcaggtaatgtatatggttgctagccgaaaaaacatttgaaattttaactgcacttttcatttatgatggttaagagcccacgcttggagtaacggaagcaccggcttagtgccgttgctatcta Intra-Species Hit: Count: 1 Min: 1 Max: 181 Len: 181 Subject: aact_AA01660_AA01661|hypothetical protein:molybdopterin biosynthesis protein|POSITIVE:POSITIVE|[1125343,1125523]|181 HSP 1 e-value: 2.0E-98 bit: 359.0 Len: 181 Query Start:1 Query End:181 Subject Strand: POSITIVE Subject Start: 1 Subject End: 181 taaccaaaatagctcgaagcagatagtgcacgtcttccaacgcatacttctagtattcaggtaatgtatatggttgctagccgaaaaaacatttgaaattttaactgcacttttcatttatgatggttaagagcccacgcttggagtaacggaagcaccggcttagtgccgttgctatcta taaccaaaatagctcgaagcagatagtgcacgtcttccaacgcatacttctagtattcaggtaatgtatatggttgctagccgaaaaaacatttgaaattttaactgcacttttcatttatgatggttaagagcccacgcttggagtaacggaagcaccggcttagtgccgttgctatcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACCAAAAUAGCUCGAAGCAGAUAGUGCACGUCUUCCAACGCAUACUUCUAGUAUUCAGGUAAUGUAUAUGGUUGCUAGCCGAAAAAACAUUUGAAAUUUUAACUGCACUUUUCAUUUAUGAUGGUUAAGAGCCCACGCUUGGAGUAACGGAAGCACCGGCUUAGUGCCGUUGCUAUCUA ...........((((.((((.((.((((((.((........(.((((.....)))).).(((...(((......)))..))).....................))))))))..)).........(((.....)))...)))).))))...(((((((.((((.....)))).)))).))). (-43.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAUAGCAACGGCACUAAGCCGGUGCUUCCGUUACUCCAAGCGUGGGCUCUUAACCAUCAUAAAUGAAAAGUGCAGUUAAAAUUUCAAAUGUUUUUUCGGCUAGCAACCAUAUACAUUACCUGAAUACUAGAAGUAUGCGUUGGAAGACGUGCACUAUCUGCUUCGAGCUAUUUUGGUUA ..(..((((.((((.....)))).))))..)......(((((....(((((.......(((..((((.....((((((..(((...(....)...)))..))).)))........))))...)))....(((((((..(((((....)))))..))).))))....)))))..)))))... (-40.22) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 381 169 122 120 65 aact:AA01486|5end_ATP_synthase_F1,_subunit_alpha_998560..998790_POSITIVE 380 179 143 48 9 aact:AA01748|5end_thiol:disulfide_interchange_protein_1183062..1183292_POSITIVE 378 176 132 66 17 aact:AA02500|5end_conserved_hypothetical_protein_1734611..1734841_POSITIVE 373 180 133 173 124 aact:AA02141|5end_ABC_transporter,_permease_protein_1464890..1465120_POSITIVE 363 179 131 64 2 aact:AA01303|5end_competence_protein_A_881996..882226_POSITIVE 354 179 133 227 166 aact:AA01054|5end_conserved_hypothetical_protein_733678..733908_POSITIVE 354 179 133 214 161 aact:AA00796|5end_iron(III)_dicitrate_transport_system_permease_protein_545686..545916_POSITIVE 354 176 133 70 26 aact:AA00052|5end_cytochrome_c-type_biogenesis_protein_precursor_40842..41072_POSITIVE 352 175 132 147 87 aact:AA02422|5end_conserved_hypothetical_protein_1668014..1668244_POSITIVE 352 175 132 147 87 aact:AA02298|5end_conserved_hypothetical_protein_1570588..1570818_POSITIVE 352 175 132 147 87 aact:AA00622|5end_conserved_hypothetical_protein_429692..429922_POSITIVE 349 168 121 73 25 aact:AA00939|5end_transcription_accessory_protein_(toxin_expression)_648332..648562_POSITIVE 345 176 134 182 123 aact:AA02179|5end_anthanilate_phosphoribosyltransferase_1489367..1489597_POSITIVE 344 173 131 76 23 aact:AA00488|5end_purine_nucleoside_phosphorylase_338075..338305_POSITIVE 343 170 121 196 146 aact:AA01586|5end_DNA_mismatch_repair_protein_1066966..1067196_POSITIVE 343 180 132 107 43 aact:AA01019|5end_conserved_hypothetical_protein_712223..712453_POSITIVE 339 178 132 57 4 aact:AA01547|5end_deoxyribose-phosphate_aldolase_1036271..1036501_POSITIVE 339 169 124 109 58 aact:AA01274|5end_hypothetical_protein_864519..864749_POSITIVE 337 170 121 142 79 aact:AA00492|5end_conserved_hypothetical_protein_343578..343808_POSITIVE 337 177 132 139 87 aact:AA00413|5end_conserved_hypothetical_protein_280142..280372_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 381 169 122 101 46 aact:AA01484|3end_ATP_synthase_F1,_subunit_gamma_998621..998771_POSITIVE 371 170 121 72 15 aact:AA01987|3end_hypothetical_protein_1350620..1350770_POSITIVE 363 179 131 83 21 aact:AA01301|3end_competence_protein_B_882095..882245_POSITIVE 354 176 133 67 23 aact:AA00050|3end_cytochrome_c-type_biogenesis_protein_precursor_(reductase)_40919..41069_POSITIVE 343 180 132 104 40 aact:AA01018|3end_conserved_hypothetical_protein_(possible_transcriptional_regulator)_712300..712450_POSITIVE 339 169 124 98 47 aact:AA01273|3end_hypothetical_protein_864588..864738_POSITIVE 338 178 132 55 14 aact:AA00474|3end_cell_division_protein_Y_(GTP-binding_signal_recognition_particle)_331724..331874_POSITIVE 333 114 71 46 1 aact:AA02286|3end_sugar_ABC_transporter,_ATP-binding_protein_1558536..1558686_POSITIVE 328 175 132 76 29 aact:AA02654|3end_probable_lipopolysaccharide_biosynthesis_protein_1849247..1849397_POSITIVE 328 177 132 67 22 aact:AA00692|3end_conserved_hypothetical_protein_474515..474665_POSITIVE 327 177 131 131 74 aact:AA02970|3end_tyrosine_phenol-lyase_2077391..2077541_POSITIVE 326 173 132 145 96 aact:AA01344|3end_magnesium_and_cobalt_transport_protein_912685..912835_POSITIVE 325 168 121 151 89 aact:AA01897|3end_conserved_hypothetical_protein_1281284..1281434_POSITIVE 323 175 137 130 100 aact:AA01432|3end_lysophospholipase_L2_961766..961916_POSITIVE 322 179 134 105 46 aact:AA01565|3end_conserved_hypothetical_protein_1050967..1051117_POSITIVE 322 176 138 121 84 aact:AA00200|3end_thymidylate_synthase_139769..139919_POSITIVE 319 179 135 90 34 aact:AA02994|3end_probable_cation_transport_protein_2093316..2093466_POSITIVE 317 140 91 53 9 aact:AA02094|3end_glycogen_phosphorylase;_glycogen_synthase_1428778..1428928_POSITIVE 307 113 71 124 87 aact:AA01937|3end_sugar_fermentation_stimulation_protein_1309334..1309484_POSITIVE 307 150 104 103 66 aact:AA01611|3end_anthranilate_synthase_component_II_1089619..1089769_POSITIVE