Origin IGS: tgatctcatcgacattttttataagtttgtgacttcgatcacataaattaattaaaaaaattagagctggatcacaaaattttatgcgcctaccaaaaaagaaaatatttgaataaattttgaacgattttatctaatccccctaaattttttatcagggaggattttttctttatacgtataagtatgattagcccgaacatataaaagacaaggtatcgagaggtttcatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aatgaaacctctcgataccttgtcttttatatgttcgggctaatcatacttatacgtataaagaaaaaatcctccctgataaaaaatttagggggattagataaaatcgttcaaaatttattcaaatattttcttttttggtaggcgcataaaattttgtgatccagctctaatttttttaattaatttatgtgatcgaagtcacaaacttataaaaaatgtcgatgagatca Mask Tandem Repeat Region ================================================ tgatctcatcgacattttttataagtttgtgacttcgatcacataaattaattaaaaaaattagagctggatcacaaaattttatgcgcctaccaaaaaagaaaatatttgaataaattttgaacgattttatctaatccccctaaattttttatcagggaggattttttctttatacgtataagtatgattagcccgaacatataaaagacaaggtatcgagaggtttcatt Find is-nt database================================================ Query_seq: AA01655:AA01656|AA01655:AA01656:hypothetical protein:maltose/maltodextrin transport ATP-binding protein:->->:1121447..1121679 233 tgatctcatcgacattttttataagtttgtgacttcgatcacataaattaattaaaaaaattagagctggatcacaaaattttatgcgcctaccaaaaaagaaaatatttgaataaattttgaacgattttatctaatccccctaaattttttatcagggaggattttttctttatacgtataagtatgattagcccgaacatataaaagacaaggtatcgagaggtttcatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01655:AA01656|AA01655:AA01656:hypothetical protein:maltose/maltodextrin transport ATP-binding protein:->->:1121447..1121679 233 tgatctcatcgacattttttataagtttgtgacttcgatcacataaattaattaaaaaaattagagctggatcacaaaattttatgcgcctaccaaaaaagaaaatatttgaataaattttgaacgattttatctaatccccctaaattttttatcagggaggattttttctttatacgtataagtatgattagcccgaacatataaaagacaaggtatcgagaggtttcatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01655:AA01656|AA01655:AA01656:hypothetical protein:maltose/maltodextrin transport ATP-binding protein:->->:1121447..1121679 233 tgatctcatcgacattttttataagtttgtgacttcgatcacataaattaattaaaaaaattagagctggatcacaaaattttatgcgcctaccaaaaaagaaaatatttgaataaattttgaacgattttatctaatccccctaaattttttatcagggaggattttttctttatacgtataagtatgattagcccgaacatataaaagacaaggtatcgagaggtttcatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgatctcatcgacattttttataagtttgtgacttcgatcacataaattaattaaaaaaattagagctggatcacaaaattttatgcgcctaccaaaaaagaaaatatttgaataaattttgaacgattttatctaatccccctaaattttttatcagggaggattttttctttatacgtataagtatgattagcccgaacatataaaagacaaggtatcgagaggtttcatt Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: - Start: 93 End: 203 ............................................................................................ W F L F Y K F L N Q V I K D L G G L N K I L S S K K K I R I L I I L G F M .............................. Protein_Len: 44 Strand: - Start: 2 End: 133 . I E D V N K I L K H S R D C L N I L F I L A P D C F K I R R G F F F I N S Y I K F S K M .................................................................................................... tgatctcatcgacattttttataagtttgtgacttcgatcacataaattaattaaaaaaattagagctggatcacaaaattttatgcgcctaccaaaaaagaaaatatttgaataaattttgaacgattttatctaatccccctaaattttttatcagggaggattttttctttatacgtataagtatgattagcccgaacatataaaagacaaggtatcgagaggtttcatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgatctcatcgacattttttataagtttgtgacttcgatcacataaattaattaaaaaaattagagctggatcacaaaattttatgcgcctaccaaaaaagaaaatatttgaataaattttgaacgattttatctaatccccctaaattttttatcagggaggattttttctttatacgtataagtatgattagcccgaacatataaaagacaaggtatcgagaggtttcatt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01655:AA01656|AA01655:AA01656:hypothetical protein:maltose/maltodextrin transport ATP-binding protein:->->:1121447..1121679 233 tgatctcatcgacattttttataagtttgtgacttcgatcacataaattaattaaaaaaattagagctggatcacaaaattttatgcgcctaccaaaaaagaaaatatttgaataaattttgaacgattttatctaatccccctaaattttttatcagggaggattttttctttatacgtataagtatgattagcccgaacatataaaagacaaggtatcgagaggtttcatt Intra-Species Hit: Count: 1 Min: 1 Max: 233 Len: 233 Subject: aact_AA01655_AA01656|hypothetical protein:maltose/maltodextrin transport ATP-binding protein|POSITIVE:POSITIVE|[1121447,1121679]|233 HSP 1 e-value: 1.0E-117 bit: 420.0 Len: 233 Query Start:1 Query End:233 Subject Strand: POSITIVE Subject Start: 1 Subject End: 233 tgatctcatcgacattttttataagtttgtgacttcgatcacataaattaattnnnnnnnttagagctggatcacaaaattttatgcgcctaccaaaaaagaaaatatttgaataaattttgaacgattttatctaatccccctaaattttttatcagggaggattttttctttatacgtataagtatgattagcccgaacatataaaagacaaggtatcgagaggtttcatt tgatctcatcgacattttttataagtttgtgacttcgatcacataaattaattaaaaaaattagagctggatcacaaaattttatgcgcctaccaaaaaagaaaatatttgaataaattttgaacgattttatctaatccccctaaattttttatcagggaggattttttctttatacgtataagtatgattagcccgaacatataaaagacaaggtatcgagaggtttcatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUCUCAUCGACAUUUUUUAUAAGUUUGUGACUUCGAUCACAUAAAUUAAUUAAAAAAAUUAGAGCUGGAUCACAAAAUUUUAUGCGCCUACCAAAAAAGAAAAUAUUUGAAUAAAUUUUGAACGAUUUUAUCUAAUCCCCCUAAAUUUUUUAUCAGGGAGGAUUUUUUCUUUAUACGUAUAAGUAUGAUUAGCCCGAACAUAUAAAAGACAAGGUAUCGAGAGGUUUCAUU .((((((.(((((.(((((((((.(((((.(.((..((((.((....((((((.....))))))...))))))........((((((...(((....((((((((..((..((.....))..))...........(((((((((............)))).)))))))))))))....)))...))))))..)).)))))).)))))))))....)..))))))))))..... (-42.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUGAAACCUCUCGAUACCUUGUCUUUUAUAUGUUCGGGCUAAUCAUACUUAUACGUAUAAAGAAAAAAUCCUCCCUGAUAAAAAAUUUAGGGGGAUUAGAUAAAAUCGUUCAAAAUUUAUUCAAAUAUUUUCUUUUUUGGUAGGCGCAUAAAAUUUUGUGAUCCAGCUCUAAUUUUUUUAAUUAAUUUAUGUGAUCGAAGUCACAAACUUAUAAAAAAUGUCGAUGAGAUCA ........(((((((((.......(((((((.(((.(((((.(((((((((((......(((((((((((((.((((((........))))))))))).(((...)))....................))))))))....)))))............))))))..)))))....................((((((....))))))))).)))))))..)))))).))).... (-44.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 254 89 49 44 4 aact:AA02141|5end_ABC_transporter,_permease_protein_1464890..1465120_POSITIVE 242 227 187 230 192 aact:AA01962|5end_recombination_protein_rec2_1326200..1326430_POSITIVE 241 197 160 148 116 aact:AA01947|5end_iron(III)-transport_ATP-binding_protein_1316390..1316620_POSITIVE 241 228 189 85 49 aact:AA00492|5end_conserved_hypothetical_protein_343578..343808_POSITIVE 237 174 134 160 116 aact:AA01956|5end_3-deoxy-manno-octulosonate_cytidylyltransferase_1322436..1322666_POSITIVE 236 199 155 85 34 aact:AA02875|5end_conserved_hypothetical_protein_(2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol_hydroxylase)_2017930..2018160_POSITIVE 235 218 171 82 38 aact:AA01305|5end_conserved_hypothetical_protein_885598..885828_POSITIVE 234 180 132 216 173 aact:AA01331|5end_50S_ribosomal_protein_L31_898408..898638_POSITIVE 231 90 66 43 19 aact:AA01393|5end_ABC_transporter,_ATP-binding_protein_940369..940599_POSITIVE 230 199 154 122 72 aact:AA02035|5end_translation_initiation_factor_IF-3_1386349..1386579_POSITIVE 228 165 121 92 37 aact:AA01850|5end_conserved_hypothetical_protein;_possible_enzyme_of_sugar_metabolism_1243710..1243940_POSITIVE 228 177 138 205 169 aact:AA01297|5end_competence_protein_E_precursor_879125..879355_POSITIVE 228 198 156 66 28 aact:AA00142|5end_glycerate_kinase_101309..101539_POSITIVE 227 227 188 140 96 aact:AA00563|5end_elongation_factor_Tu_386507..386737_POSITIVE 225 208 170 85 46 aact:AA02940|5end_conserved_hypothetical_protein_2057922..2058152_POSITIVE 225 199 152 200 152 aact:AA01623|5end_alanine_racemase_1_1097077..1097307_POSITIVE 222 97 61 218 174 aact:AA01974|5end_conserved_hypothetical_protein_1335891..1336121_POSITIVE 221 227 182 67 12 aact:AA01799|5end_polysacharide_biosynthesis_protein_(Orf14_of_cluster)_1212459..1212689_POSITIVE 220 199 157 63 18 aact:AA02157|5end_TonB-dependent_outer_membrane_receptor_1475251..1475481_POSITIVE 217 127 84 82 33 aact:AA02468|5end_conserved_hypothetical_protein_1705851..1706081_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 257 200 152 62 10 aact:AA01982|3end_maltodextrin_phosphorylase_1341854..1342004_POSITIVE 237 109 71 43 2 aact:AA01705|3end_beta-galactosidase_1157980..1158130_POSITIVE 236 89 60 39 10 aact:AA02140|3end_ABC_transporter_ATP-binding_protein_1464965..1465115_POSITIVE 234 180 132 150 107 aact:AA01330|3end_LOS_biosynthesis_enzyme_(beta-1,4-galactosyltransferase)_898422..898572_POSITIVE 232 159 116 83 41 aact:AA00010|3end_transposase_orfB_fragment_6297..6447_POSITIVE 220 199 157 58 13 aact:AA02156|3end_conserved_hypothetical_protein_1475326..1475476_POSITIVE 218 94 61 144 110 aact:AA00516|3end_autoinducer-2_production_protein_356514..356664_POSITIVE 217 127 84 69 20 aact:AA02466|3end_N-acetylneuraminate_lyase_1705918..1706068_POSITIVE 216 50 4 68 12 aact:AA01581|3end_possible_GTP-binding_protein_1065518..1065668_POSITIVE 215 228 181 136 87 aact:AA01001|3end_conserved_hypothetical_protein_699974..700124_POSITIVE 209 219 172 92 49 aact:AA02028|3end_conserved_hypothetical_protein_1382010..1382160_POSITIVE 209 229 194 39 5 aact:AA00131|3end_transcriptional_regulator_(MerR_family)_94984..95134_POSITIVE 205 163 121 106 55 aact:AA00925|3end_hypothetical_protein_635563..635713_POSITIVE 201 199 152 41 1 aact:AA01129|3end_hypothetical_protein_774788..774938_POSITIVE 199 180 138 40 3 aact:AA01648|3end_alpha-amylase_protein;_1,4-alpha-D-glucan_glucanohydrolase_1117298..1117448_POSITIVE 199 91 63 149 126 aact:AA01476|3end_hypothetical_protein_992261..992411_POSITIVE 197 216 178 42 9 aact:AA02367|3end_conserved_hypothetical_protein_1623580..1623730_POSITIVE 197 228 183 129 85 aact:AA00826|3end_UDP-3-O-acyl-GlcNAc_deacetylase_569372..569522_POSITIVE 197 160 117 131 90 aact:AA00696|3end_iron(III)_ABC_transporter,_periplasmic_iron-compound-binding_protein_477106..477256_POSITIVE 195 227 181 126 85 aact:AA00179|3end_conserved_hypothetical_protein_(possible_outer_membrane_protein)_127725..127875_POSITIVE