Origin IGS: gttgtaagtccttgttggttatttactgataccggagcgaatacgttgagccgcttcgtccggtgcctgtttaacggtttgacgttcggtaactgcaagttga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tcaacttgcagttaccgaacgtcaaaccgttaaacaggcaccggacgaagcggctcaacgtattcgctccggtatcagtaaataaccaacaaggacttacaac Mask Tandem Repeat Region ================================================ gttgtaagtccttgttggttatttactgataccggagcgaatacgttgagccgcttcgtccggtgcctgtttaacggtttgacgttcggtaactgcaagttga Find is-nt database================================================ Query_seq: AA01654:AA01655|AA01654:AA01655:periplasmic maltose-binding protein precursor:hypothetical protein:->->:1121224..1121326 103 gttgtaagtccttgttggttatttactgataccggagcgaatacgttgagccgcttcgtccggtgcctgtttaacggtttgacgttcggtaactgcaagttga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01654:AA01655|AA01654:AA01655:periplasmic maltose-binding protein precursor:hypothetical protein:->->:1121224..1121326 103 gttgtaagtccttgttggttatttactgataccggagcgaatacgttgagccgcttcgtccggtgcctgtttaacggtttgacgttcggtaactgcaagttga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01654:AA01655|AA01654:AA01655:periplasmic maltose-binding protein precursor:hypothetical protein:->->:1121224..1121326 103 gttgtaagtccttgttggttatttactgataccggagcgaatacgttgagccgcttcgtccggtgcctgtttaacggtttgacgttcggtaactgcaagttga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gttgtaagtccttgttggttatttactgataccggagcgaatacgttgagccgcttcgtccggtgcctgtttaacggtttgacgttcggtaactgcaagttga Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: - Start: 3 End: 98 .. T L G Q Q N N V S V P A F V N L R K T R H R N L P K V N P L Q M ..... gttgtaagtccttgttggttatttactgataccggagcgaatacgttgagccgcttcgtccggtgcctgtttaacggtttgacgttcggtaactgcaagttga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gttgtaagtccttgttggttatttactgataccggagcgaatacgttgagccgcttcgtccggtgcctgtttaacggtttgacgttcggtaactgcaagttga Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01654:AA01655|AA01654:AA01655:periplasmic maltose-binding protein precursor:hypothetical protein:->->:1121224..1121326 103 gttgtaagtccttgttggttatttactgataccggagcgaatacgttgagccgcttcgtccggtgcctgtttaacggtttgacgttcggtaactgcaagttga Intra-Species Hit: Count: 1 Min: 1 Max: 103 Len: 103 Subject: aact_AA01654_AA01655|periplasmic maltose-binding protein precursor:hypothetical protein|POSITIVE:POSITIVE|[1121224,1121326]|103 HSP 1 e-value: 4.0E-52 bit: 204.0 Len: 103 Query Start:1 Query End:103 Subject Strand: POSITIVE Subject Start: 1 Subject End: 103 gttgtaagtccttgttggttatttactgataccggagcgaatacgttgagccgcttcgtccggtgcctgtttaacggtttgacgttcggtaactgcaagttga gttgtaagtccttgttggttatttactgataccggagcgaatacgttgagccgcttcgtccggtgcctgtttaacggtttgacgttcggtaactgcaagttga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUGUAAGUCCUUGUUGGUUAUUUACUGAUACCGGAGCGAAUACGUUGAGCCGCUUCGUCCGGUGCCUGUUUAACGGUUUGACGUUCGGUAACUGCAAGUUGA ...((((((((.....))..))))))...(((((((((((((.((((((((.((.((....)).))..))))))))))))).).)))))))............ (-26.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCAACUUGCAGUUACCGAACGUCAAACCGUUAAACAGGCACCGGACGAAGCGGCUCAACGUAUUCGCUCCGGUAUCAGUAAAUAACCAACAAGGACUUACAAC ....((((..((((...((((......)))).....(..(((((((((((((......))).)))).))))))..)......))))...)))).......... (-25.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 417 76 32 125 78 aact:AA00603|5end_undecaprenyl_pyrophosphate_synthetase_(UPPS)_410283..410513_POSITIVE 412 81 42 199 152 aact:AA02038|5end_50S_ribosomal_protein_L35_1387052..1387282_POSITIVE 399 89 43 203 147 aact:AA00991|5end_conserved_hypothetical_protein_693191..693421_POSITIVE 398 90 44 168 120 aact:AA02218|5end_deoxyribose-phosphate_aldolase_(fragment?)_1509758..1509988_POSITIVE 380 80 33 211 158 aact:AA00790|5end_recombination_DNA_repair_protein_542455..542685_POSITIVE 376 67 26 224 163 aact:AA01957|5end_conserved_hypothetical_protein_1323211..1323441_POSITIVE 368 67 26 205 168 aact:AA01421|5end_phosphoglycolate_phosphatase_955055..955285_POSITIVE 366 67 23 80 40 aact:AA00574|5end_regulatory_protein_392113..392343_POSITIVE 363 96 51 200 153 aact:AA00612|5end_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosami_ne_417840..418070_POSITIVE 358 78 32 64 9 aact:AA00309|5end_3-hydroxyisobutyrate_dehydrogenase;_2-hydroxy-3-oxopropionate_reductase_209285..209515_POSITIVE 352 90 45 52 1 aact:AA01185|5end_conserved_hypothetical_protein_(possible_membrane_protein)_811051..811281_POSITIVE 352 89 44 88 53 aact:AA00601|5end_hypothetical_protein_408863..409093_POSITIVE 351 80 33 69 28 aact:AA02190|5end_hydrogenase-2_component_protein_1492919..1493149_POSITIVE 351 80 33 203 157 aact:AA00992|5end_ribonuclease_BN_693661..693891_POSITIVE 347 90 44 205 150 aact:AA00738|5end_hypothetical_protein_505087..505317_POSITIVE 344 100 52 67 10 aact:AA02205|5end_short_chain_dehydrogenase/reductase_1501170..1501400_POSITIVE 344 97 51 192 134 aact:AA01234|5end_50S_ribosomal_protein_L18_841400..841630_POSITIVE 344 90 41 228 174 aact:AA00311|5end_L-fuculose-phosphate_aldolase_211434..211664_POSITIVE 344 76 32 199 154 aact:AA00207|5end_D-ribose_ABC_transporter,_ATP-binding_protein_/_galactoside_ABC_transporter,_ATP-binding_protein_142592..142822_POSITIVE 343 67 25 151 116 aact:AA01276|5end_heptosyltransferase_III_864730..864960_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 422 78 33 141 72 aact:AA02584|3end_ADP-heptose_synthase_1798729..1798879_POSITIVE 412 81 42 119 72 aact:AA02037|3end_hypothetical_protein_1387052..1387202_POSITIVE 392 96 51 43 2 aact:AA01269|3end_hypothetical_protein_863413..863563_POSITIVE 371 70 25 146 87 aact:AA02217|3end_deoxyribose-phosphate_aldolase_1509835..1509985_POSITIVE 370 90 44 65 11 aact:AA02204|3end_hypothetical_protein_1501262..1501412_POSITIVE 359 90 44 65 5 aact:AA00758|3end_hypothetical_protein_520696..520846_POSITIVE 352 90 45 54 3 aact:AA01184|3end_hypothetical_protein_811133..811283_POSITIVE 352 89 44 84 49 aact:AA00600|3end_ribosome_recycling_factor_(ribosome_releasing_factor)_408939..409089_POSITIVE 338 77 33 148 102 aact:AA00408|3end_SUN_protein_(FMU_protein)_273688..273838_POSITIVE 332 89 49 81 45 aact:AA00530|3end_hypothetical_protein_362668..362818_POSITIVE 331 76 32 76 21 aact:AA00211|3end_hypothetical_protein_146163..146313_POSITIVE 329 90 43 150 91 aact:AA01367|3end_tRNA_(Guanine-N(1)-)-methyltransferase_(M1G-methyltransferase)_924536..924686_POSITIVE 328 86 44 145 105 aact:AA00332|3end_PTS_system,_fructose-specific_IIA/FPr_component_(diphosphoryl_transfer_protein)_225617..225767_POSITIVE 327 70 31 151 111 aact:AA00316|3end_conserved_hypothetical_protein_215340..215490_POSITIVE 321 65 21 75 12 aact:AA02696|3end_formate_hydrogenlyase_maturation_protein_1879957..1880107_POSITIVE 321 80 33 120 71 aact:AA02440|3end_ABC_transporter,_ATP-binding_protein_1685791..1685941_POSITIVE 320 89 45 118 81 aact:AA02959|3end_pyrroline-5-carboxylate_(P5C)_reductase_2070976..2071126_POSITIVE 317 90 42 138 94 aact:AA00974|3end_glucosamine-fructose-6-phosphate_681127..681277_POSITIVE 316 96 53 107 49 aact:AA02969|3end_beta_tyrosinase_(tyrosine_phenol-lyase)_2076417..2076567_POSITIVE 315 78 32 76 7 aact:AA01078|3end_transcriptional_regulator_(probable_MerR_family)_747453..747603_POSITIVE