Origin IGS: agtatttacctatttaacttaactatttatgactaacaggaggagttgtgggttttactggagcagactgttgttccctaacctttatattaaaatcatctgactttaatttatagttttctgtggataatctttcatttgtggttcttaatgttataccatccattacaatggcttcatggttttggttatatctcatatcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagatatgagatataaccaaaaccatgaagccattgtaatggatggtataacattaagaaccacaaatgaaagattatccacagaaaactataaattaaagtcagatgattttaatataaaggttagggaacaacagtctgctccagtaaaacccacaactcctcctgttagtcataaatagttaagttaaataggtaaatact Mask Tandem Repeat Region ================================================ agtatttacctatttaacttaactatttatgactaacaggaggagttgtgggttttactggagcagactgttgttccctaacctttatattaaaatcatctgactttaatttatagttttctgtggataatctttcatttgtggttcttaatgttataccatccattacaatggcttcatggttttggttatatctcatatcta Find is-nt database================================================ Query_seq: AA01635:AA01636|AA01635:AA01636:ribonucleoside diphosphate reductase, beta chain:conserved hypothetical protein:->->:1105883..1106086 204 agtatttacctatttaacttaactatttatgactaacaggaggagttgtgggttttactggagcagactgttgttccctaacctttatattaaaatcatctgactttaatttatagttttctgtggataatctttcatttgtggttcttaatgttataccatccattacaatggcttcatggttttggttatatctcatatcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01635:AA01636|AA01635:AA01636:ribonucleoside diphosphate reductase, beta chain:conserved hypothetical protein:->->:1105883..1106086 204 agtatttacctatttaacttaactatttatgactaacaggaggagttgtgggttttactggagcagactgttgttccctaacctttatattaaaatcatctgactttaatttatagttttctgtggataatctttcatttgtggttcttaatgttataccatccattacaatggcttcatggttttggttatatctcatatcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01635:AA01636|AA01635:AA01636:ribonucleoside diphosphate reductase, beta chain:conserved hypothetical protein:->->:1105883..1106086 204 agtatttacctatttaacttaactatttatgactaacaggaggagttgtgggttttactggagcagactgttgttccctaacctttatattaaaatcatctgactttaatttatagttttctgtggataatctttcatttgtggttcttaatgttataccatccattacaatggcttcatggttttggttatatctcatatcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agtatttacctatttaacttaactatttatgactaacaggaggagttgtgggttttactggagcagactgttgttccctaacctttatattaaaatcatctgactttaatttatagttttctgtggataatctttcatttgtggttcttaatgttataccatccattacaatggcttcatggttttggttatatctcatatcta Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 100 End: 204 ................................................................................................... M T L I Y S F L W I I F H L W F L M L Y H P L Q W L H G F G Y I S Y L Protein_Len: 58 Strand: - Start: 26 End: 199 ......................... K H S V P P T T P K V P A S Q Q E R V K I N F D D S K L K Y N E T S L R E N T T R L T I G D M V I A E H N Q N Y R M ..... agtatttacctatttaacttaactatttatgactaacaggaggagttgtgggttttactggagcagactgttgttccctaacctttatattaaaatcatctgactttaatttatagttttctgtggataatctttcatttgtggttcttaatgttataccatccattacaatggcttcatggttttggttatatctcatatcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agtatttacctatttaacttaactatttatgactaacaggaggagttgtgggttttactggagcagactgttgttccctaacctttatattaaaatcatctgactttaatttatagttttctgtggataatctttcatttgtggttcttaatgttataccatccattacaatggcttcatggttttggttatatctcatatcta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01635:AA01636|AA01635:AA01636:ribonucleoside diphosphate reductase, beta chain:conserved hypothetical protein:->->:1105883..1106086 204 agtatttacctatttaacttaactatttatgactaacaggaggagttgtgggttttactggagcagactgttgttccctaacctttatattaaaatcatctgactttaatttatagttttctgtggataatctttcatttgtggttcttaatgttataccatccattacaatggcttcatggttttggttatatctcatatcta Intra-Species Hit: Count: 1 Min: 1 Max: 204 Len: 204 Subject: aact_AA01635_AA01636|ribonucleoside diphosphate reductase, beta chain:conserved hypothetical protein|POSITIVE:POSITIVE|[1105883,1106086]|204 HSP 1 e-value: 1.0E-112 bit: 404.0 Len: 204 Query Start:1 Query End:204 Subject Strand: POSITIVE Subject Start: 1 Subject End: 204 agtatttacctatttaacttaactatttatgactaacaggaggagttgtgggttttactggagcagactgttgttccctaacctttatattaaaatcatctgactttaatttatagttttctgtggataatctttcatttgtggttcttaatgttataccatccattacaatggcttcatggttttggttatatctcatatcta agtatttacctatttaacttaactatttatgactaacaggaggagttgtgggttttactggagcagactgttgttccctaacctttatattaaaatcatctgactttaatttatagttttctgtggataatctttcatttgtggttcttaatgttataccatccattacaatggcttcatggttttggttatatctcatatcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUAUUUACCUAUUUAACUUAACUAUUUAUGACUAACAGGAGGAGUUGUGGGUUUUACUGGAGCAGACUGUUGUUCCCUAACCUUUAUAUUAAAAUCAUCUGACUUUAAUUUAUAGUUUUCUGUGGAUAAUCUUUCAUUUGUGGUUCUUAAUGUUAUACCAUCCAUUACAAUGGCUUCAUGGUUUUGGUUAUAUCUCAUAUCUA ((((...((((((..(((((..((.((.......)).))...)))))))))))..))))(((((((....))))))).(((((...(((((((((((((.(((.....((((((((....))))))))......)))...)))))).)))))))...(((((((((....))))....)))))...)))))............. (-38.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAUAUGAGAUAUAACCAAAACCAUGAAGCCAUUGUAAUGGAUGGUAUAACAUUAAGAACCACAAAUGAAAGAUUAUCCACAGAAAACUAUAAAUUAAAGUCAGAUGAUUUUAAUAUAAAGGUUAGGGAACAACAGUCUGCUCCAGUAAAACCCACAACUCCUCCUGUUAGUCAUAAAUAGUUAAGUUAAAUAGGUAAAUACU .......(((...(((((...((.(((....))).))..((((((((....((((..........))))....)))))))).............(((((((((....))))))))).....))))).(((.((......)).)))..............)))..((((((...(.(((....))).)...))))))........ (-29.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 333 79 45 198 167 aact:AA02416|5end_conserved_hypothetical_protein_1658920..1659150_POSITIVE 301 75 39 69 22 aact:AA01355|5end_large_subunit_pseudouridylate_synthase_D_917228..917458_POSITIVE 282 190 142 110 57 aact:AA02950|5end_isoleucyl-tRNA_synthetase_2062295..2062525_POSITIVE 261 65 29 107 75 aact:AA02731|5end_outer_membrane_lipoprotein_carrier_precursor_1911498..1911728_POSITIVE 258 78 46 44 1 aact:AA01190|5end_glycosyl_transferase_(possible_dolichol-phosphate_mannosyltransferase)_815278..815508_POSITIVE 257 80 39 60 2 aact:AA01566|5end_conserved_hypothetical_protein_1051811..1052041_POSITIVE 257 80 39 60 2 aact:AA01261|5end_conserved_hypothetical_protein_856443..856673_POSITIVE 256 80 37 103 65 aact:AA00452|5end_phosphomannomutase;_phosphoglucomutase_315288..315518_POSITIVE 255 79 31 151 95 aact:AA02911|5end_hypothetical_protein_2039006..2039236_POSITIVE 255 73 31 160 119 aact:AA00863|5end_conserved_hypothetical_protein_596679..596909_POSITIVE 251 163 122 171 132 aact:AA02254|5end_2-oxoglutarate/malate_translocator_1534846..1535076_POSITIVE 251 78 31 164 89 aact:AA01650|5end_maltose_transport_inner_membrane_protein_1118228..1118458_POSITIVE 249 75 37 70 18 aact:AA01488|5end_ATP_synthase_F0,_subunit_b_1000676..1000906_POSITIVE 248 79 39 86 30 aact:AA02419|5end_hypothetical_protein_1661731..1661961_POSITIVE 247 68 21 63 27 aact:AA00686|5end_hypothetical_protein_468852..469082_POSITIVE 247 67 42 46 14 aact:AA00212|5end_ribokinase_146056..146286_POSITIVE 243 84 41 149 103 aact:AA02271|5end_hypothetical_protein_1548623..1548853_POSITIVE 242 62 30 68 36 aact:AA01997|5end_probable_translation_factor_1356164..1356394_POSITIVE 241 83 45 58 21 aact:AA01306|5end_glutathione_reductase_886538..886768_POSITIVE 241 75 32 163 105 aact:AA00550|5end_ferredoxin-type_protein_376444..376674_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 333 79 45 119 88 aact:AA02414|3end_hypothetical_protein_1658921..1659071_POSITIVE 301 75 39 82 35 aact:AA01354|3end_conserved_hypothetical_protein_917321..917471_POSITIVE 292 179 141 121 83 aact:AA00419|3end_conserved_hypothetical_protein_(possible_phosphatidylethanolamine-binding_protein)_284911..285061_POSITIVE 284 66 37 143 114 aact:AA02100|3end_lipoprotein_1430759..1430909_POSITIVE 262 77 39 142 94 aact:AA00662|3end_cystathionine_beta-lyase_455527..455677_POSITIVE 261 65 29 91 59 aact:AA02728|3end_conserved_hypothetical_protein_(possible_ATPase)_1911562..1911712_POSITIVE 258 78 46 44 1 aact:AA01189|3end_conserved_hypothetical_protein_(possible_glycosyl_transferase)_815358..815508_POSITIVE 256 80 37 83 45 aact:AA00451|3end_hypothetical_protein_315348..315498_POSITIVE 255 73 31 116 75 aact:AA00862|3end_conserved_hypothetical_protein_596715..596865_POSITIVE 251 78 31 139 64 aact:AA01649|3end_maltose_ABC_transporter,_permease_protein_1118283..1118433_POSITIVE 249 75 37 53 1 aact:AA01487|3end_ATP_synthase_F1,_subunit_delta_1000739..1000889_POSITIVE 248 79 39 86 30 aact:AA02418|3end_peptidyl-tRNA_hydrolase_1661811..1661961_POSITIVE 247 67 42 73 41 aact:AA00211|3end_hypothetical_protein_146163..146313_POSITIVE 243 84 41 114 68 aact:AA02270|3end_endopeptidase_La;_lon_protease;_protease_La_homolog;_ATP-dependent_protease_LA_(lon-1)_1548668..1548818_POSITIVE 236 83 41 78 38 aact:AA00324|3end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_221321..221471_POSITIVE 235 78 34 148 102 aact:AA02005|3end_vancomycin_sensitivity;_SanA_protein_1364020..1364170_POSITIVE 235 68 30 51 11 aact:AA01942|3end_hypothetical_protein_1312953..1313103_POSITIVE 234 60 39 73 54 aact:AA02632|3end_rhamnosyltransferase_1832694..1832844_POSITIVE 234 67 30 113 71 aact:AA02590|3end_conserved_hypothetical_protein_1801642..1801792_POSITIVE 230 77 36 46 2 aact:AA01814|3end_endonuclease_III_1222593..1222743_POSITIVE