Origin IGS: taaggtcgagtgcgtgtaaacgcacttttttaatttatggatggtgcaacaagttgtaccttccaaagtgcggtcgtttttttactcgtaaatcctgaagatttactcgctccttcggaactgttggcagagacaacgttcaaaaagcgttgctttttgtctgatattttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaaatatcagacaaaaagcaacgctttttgaacgttgtctctgccaacagttccgaaggagcgagtaaatcttcaggatttacgagtaaaaaaacgaccgcactttggaaggtacaacttgttgcaccatccataaattaaaaaagtgcgtttacacgcactcgacctta Mask Tandem Repeat Region ================================================ taaggtcgagtgcgtgtaaacgcacttttttaatttatggatggtgcaacaagttgtaccttccaaagtgcggtcgtttttttactcgtaaatcctgaagatttactcgctccttcggaactgttggcagagacaacgttcaaaaagcgttgctttttgtctgatattttta Find is-nt database================================================ Query_seq: AA01631:AA01633|AA01631:AA01633:dihydrodipicolinate reductase:probable ferredoxin-like protein:->->:1103976..1104147 172 taaggtcgagtgcgtgtaaacgcacttttttaatttatggatggtgcaacaagttgtaccttccaaagtgcggtcgtttttttactcgtaaatcctgaagatttactcgctccttcggaactgttggcagagacaacgttcaaaaagcgttgctttttgtctgatattttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01631:AA01633|AA01631:AA01633:dihydrodipicolinate reductase:probable ferredoxin-like protein:->->:1103976..1104147 172 taaggtcgagtgcgtgtaaacgcacttttttaatttatggatggtgcaacaagttgtaccttccaaagtgcggtcgtttttttactcgtaaatcctgaagatttactcgctccttcggaactgttggcagagacaacgttcaaaaagcgttgctttttgtctgatattttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01631:AA01633|AA01631:AA01633:dihydrodipicolinate reductase:probable ferredoxin-like protein:->->:1103976..1104147 172 taaggtcgagtgcgtgtaaacgcacttttttaatttatggatggtgcaacaagttgtaccttccaaagtgcggtcgtttttttactcgtaaatcctgaagatttactcgctccttcggaactgttggcagagacaacgttcaaaaagcgttgctttttgtctgatattttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaggtcgagtgcgtgtaaacgcacttttttaatttatggatggtgcaacaagttgtaccttccaaagtgcggtcgtttttttactcgtaaatcctgaagatttactcgctccttcggaactgttggcagagacaacgttcaaaaagcgttgctttttgtctgatattttta Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: + Start: 37 End: 171 .................................... M D G A T S C T F Q S A V V F L L V N P E D L L A P S E L L A E T T F K K R C F L S D I F . Protein_Len: 33 Strand: - Start: 38 End: 136 ..................................... P H H L L N Y R G F H P R K K V R L D Q L N V R E K P V T P L S M .................................... Protein_Len: 45 Strand: - Start: 33 End: 167 ................................ N I S P A V L Q V K W L A T T K K S T F G S S K S A G E S S N A S V V N L F R Q K K D S M ..... taaggtcgagtgcgtgtaaacgcacttttttaatttatggatggtgcaacaagttgtaccttccaaagtgcggtcgtttttttactcgtaaatcctgaagatttactcgctccttcggaactgttggcagagacaacgttcaaaaagcgttgctttttgtctgatattttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaggtcgagtgcgtgtaaacgcacttttttaatttatggatggtgcaacaagttgtaccttccaaagtgcggtcgtttttttactcgtaaatcctgaagatttactcgctccttcggaactgttggcagagacaacgttcaaaaagcgttgctttttgtctgatattttta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 59 HP_score: -5.3 Tail_Score: -3.60416 Start: 134 End: 152 Full_Region: AACTGTTGGCAGAGA CAACGTT CAAAA AGCGTTG CTTTTTGTCTGATAT .....................................................................................................................................CAACGTTCAAAAAGCGTTG.................... Find igs database================================================ Query_seq: AA01631:AA01633|AA01631:AA01633:dihydrodipicolinate reductase:probable ferredoxin-like protein:->->:1103976..1104147 172 taaggtcgagtgcgtgtaaacgcacttttttaatttatggatggtgcaacaagttgtaccttccaaagtgcggtcgtttttttactcgtaaatcctgaagatttactcgctccttcggaactgttggcagagacaacgttcaaaaagcgttgctttttgtctgatattttta Intra-Species Hit: Count: 1 Min: 1 Max: 172 Len: 172 Subject: aact_AA01631_AA01633|dihydrodipicolinate reductase:probable ferredoxin-like protein|POSITIVE:POSITIVE|[1103976,1104147]|172 HSP 1 e-value: 2.0E-80 bit: 299.0 Len: 172 Query Start:1 Query End:172 Subject Strand: POSITIVE Subject Start: 1 Subject End: 172 taaggtcgagtgcgtgtaaacgcacttttttaatttatggatggtgcaacaagttgtaccttccaaagtgcggtcgnnnnnnnactcgtaaatcctgaagatttactcgctccttcggaactgttggcagagacaacgttcaaaaagcgttgctttttgtctgatattttta taaggtcgagtgcgtgtaaacgcacttttttaatttatggatggtgcaacaagttgtaccttccaaagtgcggtcgtttttttactcgtaaatcctgaagatttactcgctccttcggaactgttggcagagacaacgttcaaaaagcgttgctttttgtctgatattttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGGUCGAGUGCGUGUAAACGCACUUUUUUAAUUUAUGGAUGGUGCAACAAGUUGUACCUUCCAAAGUGCGGUCGUUUUUUUACUCGUAAAUCCUGAAGAUUUACUCGCUCCUUCGGAACUGUUGGCAGAGACAACGUUCAAAAAGCGUUGCUUUUUGUCUGAUAUUUUUA .((((..((((((((....))))))))..........((((.(((((((....))))))).))))....(((...((......))..(((((((.....))))))).))).)))).((((.(((..((((((((((((((.....))))))).)))))).)..))).)))). (-53.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAAUAUCAGACAAAAAGCAACGCUUUUUGAACGUUGUCUCUGCCAACAGUUCCGAAGGAGCGAGUAAAUCUUCAGGAUUUACGAGUAAAAAAACGACCGCACUUUGGAAGGUACAACUUGUUGCACCAUCCAUAAAUUAAAAAAGUGCGUUUACACGCACUCGACCUUA .............((((((((...))))))))...((.(((..........(((((...)))))..(((((((.....)))))))............))).))....((((.(((.(((....))).))).))))...........(((((((....)))))))........ (-43.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 628 150 107 186 143 aact:AA00054|5end_cytochrome_c_biogenesis_protein_41393..41623_POSITIVE 551 149 107 208 165 aact:AA02220|5end_ABC_transport_system_permease_protein_1511155..1511385_POSITIVE 547 84 42 199 143 aact:AA01175|5end_6-phosphogluconate_dehydrogenase_804864..805094_POSITIVE 534 160 112 179 129 aact:AA01079|5end_conserved_hypothetical_protein_747404..747634_POSITIVE 497 128 86 231 186 aact:AA00096|5end_L-asparaginase_II_precursor_71280..71510_POSITIVE 495 150 107 65 21 aact:AA01953|5end_hypothetical_protein_1320132..1320362_POSITIVE 467 150 101 143 94 aact:AA00240|5end_hypothetical_protein_171390..171620_POSITIVE 447 150 101 226 172 aact:AA01936|5end_tyrosyl-tRNA_synthetase_1307043..1307273_POSITIVE 415 145 102 169 118 aact:AA02728|5end_conserved_hypothetical_protein_(possible_ATPase)_1910145..1910375_POSITIVE 378 110 65 151 99 aact:AA01059|5end_orotate_phosphoribosyltransferase_735052..735282_POSITIVE 374 89 41 178 124 aact:AA01173|5end_VapD-homolog_803850..804080_POSITIVE 353 89 42 60 15 aact:AA00582|5end_conserved_hypothetical_protein_397904..398134_POSITIVE 344 88 56 176 149 aact:AA02410|5end_cell_division_protein_1655429..1655659_POSITIVE 341 89 43 208 157 aact:AA02476|5end_conserved_hypothetical_protein_1711610..1711840_POSITIVE 341 89 43 78 27 aact:AA02475|5end_hypothetical_protein_1711480..1711710_POSITIVE 337 76 34 87 37 aact:AA01892|5end_hypothetical_protein_1276688..1276918_POSITIVE 335 90 42 164 100 aact:AA01544|5end_conserved_hypothetical_protein_1034265..1034495_POSITIVE 335 90 42 74 10 aact:AA01543|5end_hypothetical_protein_1034175..1034405_POSITIVE 331 158 116 184 142 aact:AA00595|5end_hypothetical_protein_407194..407424_POSITIVE 326 80 38 109 53 aact:AA02097|5end_DedA-family_integral_membrane_protein_1428823..1429053_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 536 140 91 56 4 aact:AA00093|3end_L-serine_dehydratase_(L-serine_deaminase)_71173..71323_POSITIVE 534 160 112 148 98 aact:AA01078|3end_transcriptional_regulator_(probable_MerR_family)_747453..747603_POSITIVE 478 160 113 53 3 aact:AA01167|3end_glucose-6-phosphate_1-dehydrogenase_801067..801217_POSITIVE 467 150 101 91 42 aact:AA00239|3end_hypothetical_protein_171418..171568_POSITIVE 447 150 101 96 42 aact:AA01934|3end_conserved_hypothetical_protein_1306993..1307143_POSITIVE 378 110 65 66 14 aact:AA01057|3end_DnaJ-like_protein_djlA_735047..735197_POSITIVE 374 89 41 95 41 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 355 70 22 150 103 aact:AA01174|3end_hypothetical_protein_804923..805073_POSITIVE 352 161 124 38 1 aact:AA01811|3end_electron_transport_complex_protein_1221033..1221183_POSITIVE 344 88 56 71 44 aact:AA02409|3end_DNA_ligase_1655404..1655554_POSITIVE 341 89 43 66 15 aact:AA02474|3end_transmembrane_protein_1711548..1711698_POSITIVE 337 76 34 103 53 aact:AA01891|3end_pyridoxine_kinase_1276784..1276934_POSITIVE 318 110 65 112 61 aact:AA00508|3end_SAM-dependent_methyltransferase_353883..354033_POSITIVE 313 69 21 58 19 aact:AA01631|3end_dihydrodipicolinate_reductase_1103915..1104065_POSITIVE 311 88 48 82 38 aact:AA01778|3end_aminopeptidase_A/I;_cytosol_aminopeptidase;_leucyl_aminopeptidase_1199965..1200115_POSITIVE 311 102 65 146 102 aact:AA01434|3end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_963021..963171_POSITIVE 310 88 48 82 32 aact:AA02251|3end_biotin_sulfoxide_reductase;_Trimethylamine-N-oxide_reductase_precursor_1533843..1533993_POSITIVE 310 161 123 141 103 aact:AA01089|3end_hypothetical_protein_753597..753747_POSITIVE 306 110 65 57 7 aact:AA03001|3end_probable_glycyl_radical_protein/pyruvate-formate_lyase_2096487..2096637_POSITIVE 304 60 12 120 77 aact:AA01880|3end_N-acetylglucosamine_repressor_1268383..1268533_POSITIVE