Origin IGS: taattcagcctgaacggcattaaagtgcggtcgaaattctctgcgttttttaaccgcactttttcctttcttactgccagaaaaggaattttatgctagaattcacgcgctatttttcattaacatatctataggaaagaac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gttctttcctatagatatgttaatgaaaaatagcgcgtgaattctagcataaaattccttttctggcagtaagaaaggaaaaagtgcggttaaaaaacgcagagaatttcgaccgcactttaatgccgttcaggctgaatta Mask Tandem Repeat Region ================================================ taattcagcctgaacggcattaaagtgcggtcgaaattctctgcgttttttaaccgcactttttcctttcttactgccagaaaaggaattttatgctagaattcacgcgctatttttcattaacatatctataggaaagaac Find is-nt database================================================ Query_seq: AA01624:AA01625|AA01624:AA01625:glucose-6-phosphate isomerase:riboflavin synthase, beta chain:->->:1099962..1100103 142 taattcagcctgaacggcattaaagtgcggtcgaaattctctgcgttttttaaccgcactttttcctttcttactgccagaaaaggaattttatgctagaattcacgcgctatttttcattaacatatctataggaaagaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01624:AA01625|AA01624:AA01625:glucose-6-phosphate isomerase:riboflavin synthase, beta chain:->->:1099962..1100103 142 taattcagcctgaacggcattaaagtgcggtcgaaattctctgcgttttttaaccgcactttttcctttcttactgccagaaaaggaattttatgctagaattcacgcgctatttttcattaacatatctataggaaagaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01624:AA01625|AA01624:AA01625:glucose-6-phosphate isomerase:riboflavin synthase, beta chain:->->:1099962..1100103 142 taattcagcctgaacggcattaaagtgcggtcgaaattctctgcgttttttaaccgcactttttcctttcttactgccagaaaaggaattttatgctagaattcacgcgctatttttcattaacatatctataggaaagaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taattcagcctgaacggcattaaagtgcggtcgaaattctctgcgttttttaaccgcactttttcctttcttactgccagaaaaggaattttatgctagaattcacgcgctatttttcattaacatatctataggaaagaac Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 41 End: 142 ........................................ M R F L T A L F P F L L P E K E F Y A R I H A L F F I N I S I G K N taattcagcctgaacggcattaaagtgcggtcgaaattctctgcgttttttaaccgcactttttcctttcttactgccagaaaaggaattttatgctagaattcacgcgctatttttcattaacatatctataggaaagaac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 83 Start: 73 End: 102 ........................................................................ACTGCCAGAAAAGGAATTTTATGCTAGAAT........................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taattcagcctgaacggcattaaagtgcggtcgaaattctctgcgttttttaaccgcactttttcctttcttactgccagaaaaggaattttatgctagaattcacgcgctatttttcattaacatatctataggaaagaac Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 53 HP_score: -3.5 Tail_Score: -3.76492 Start: 25 End: 59 Full_Region: gtaagaaaggaaaaa gtgcggttaaaaaac gcaga gaatttcgaccgcac tttaatgccgttcag ........................gtgcggttaaaaaacgcagagaatttcgaccgcac................................................................................... Find igs database================================================ Query_seq: AA01624:AA01625|AA01624:AA01625:glucose-6-phosphate isomerase:riboflavin synthase, beta chain:->->:1099962..1100103 142 taattcagcctgaacggcattaaagtgcggtcgaaattctctgcgttttttaaccgcactttttcctttcttactgccagaaaaggaattttatgctagaattcacgcgctatttttcattaacatatctataggaaagaac Intra-Species Hit: Count: 1 Min: 1 Max: 142 Len: 142 Subject: aact_AA01624_AA01625|glucose-6-phosphate isomerase:riboflavin synthase, beta chain|POSITIVE:POSITIVE|[1099962,1100103]|142 HSP 1 e-value: 3.0E-75 bit: 281.0 Len: 142 Query Start:1 Query End:142 Subject Strand: POSITIVE Subject Start: 1 Subject End: 142 taattcagcctgaacggcattaaagtgcggtcgaaattctctgcgttttttaaccgcactttttcctttcttactgccagaaaaggaattttatgctagaattcacgcgctatttttcattaacatatctataggaaagaac taattcagcctgaacggcattaaagtgcggtcgaaattctctgcgttttttaaccgcactttttcctttcttactgccagaaaaggaattttatgctagaattcacgcgctatttttcattaacatatctataggaaagaac Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUCAGCCUGAACGGCAUUAAAGUGCGGUCGAAAUUCUCUGCGUUUUUUAACCGCACUUUUUCCUUUCUUACUGCCAGAAAAGGAAUUUUAUGCUAGAAUUCACGCGCUAUUUUUCAUUAACAUAUCUAUAGGAAAGAAC .......((.((((.(((((.((((((((((..(((..(.....)..)))..))))))))))(((((((((.......)).)))))))....)))))....)))).))....((((((..((.......))...)))))).. (-32.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUCUUUCCUAUAGAUAUGUUAAUGAAAAAUAGCGCGUGAAUUCUAGCAUAAAAUUCCUUUUCUGGCAGUAAGAAAGGAAAAAGUGCGGUUAAAAAACGCAGAGAAUUUCGACCGCACUUUAAUGCCGUUCAGGCUGAAUUA ((((..............((((.(....).))))((.(((((....((((....(((((((.((.......)))))))))((((((((((..(((..(.....)..)))..)))))))))).)))).))))).)).)))).. (-34.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 510 67 21 231 183 aact:AA02476|5end_conserved_hypothetical_protein_1711610..1711840_POSITIVE 510 67 21 101 53 aact:AA02475|5end_hypothetical_protein_1711480..1711710_POSITIVE 494 64 21 160 117 aact:AA00759|5end_3-oxoacyl-[acyl-carrier-protein]_synthase_I_520603..520833_POSITIVE 489 69 22 196 144 aact:AA02722|5end_cytidine_deaminase_1905379..1905609_POSITIVE 474 64 23 42 1 aact:AA00758|5end_hypothetical_protein_520485..520715_POSITIVE 466 60 16 138 95 aact:AA03010|5end_thiophene_and_furan_oxidation_protein_2101102..2101332_POSITIVE 461 65 21 159 114 aact:AA02993|5end_6-phosphofructokinase_2091766..2091996_POSITIVE 461 65 21 157 113 aact:AA02113|5end_conserved_hypothetical_protein_1442183..1442413_POSITIVE 459 62 22 142 102 aact:AA02288|5end_conserved_hypothetical_protein_1558590..1558820_POSITIVE 449 62 22 149 109 aact:AA02010|5end_peptide_methionine_sulfoxide_reductase;_cytochrome_C-TYPE_biogenesis_protein;_transcriptional_regulator_1367180..1367410_POSITIVE 449 49 6 188 135 aact:AA00337|5end_conserved_hypothetical_protein_228264..228494_POSITIVE 446 70 21 163 115 aact:AA02864|5end_glycerol-3-phosphate_transport_protein_2008784..2009014_POSITIVE 434 65 22 122 79 aact:AA02362|5end_conserved_hypothetical_protein_1619905..1620135_POSITIVE 433 64 22 154 112 aact:AA00124|5end_conserved_hypothetical_protein_(possible_cytoplasmic_protein)_90270..90500_POSITIVE 433 64 22 105 63 aact:AA00123|5end_hypothetical_protein_90221..90451_POSITIVE 433 64 22 96 54 aact:AA00122|5end_hypothetical_protein_90212..90442_POSITIVE 429 58 15 158 111 aact:AA00625|5end_hypothetical_protein_430565..430795_POSITIVE 429 58 15 50 3 aact:AA00623|5end_hypothetical_protein_430457..430687_POSITIVE 428 65 22 161 117 aact:AA01085|5end_mutator_mutT_protein_(7,8-dihydro-8-oxoguanine-triphosphatase)_(8-oxo-dGTPase)_(dGTP_pyrophosphohydrolase)_750970..751200_POSITIVE 426 65 21 208 164 aact:AA02754|5end_conserved_hypothetical_protein_1928949..1929179_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 582 64 22 88 46 aact:AA02280|3end_conserved_hypothetical_protein_1552767..1552917_POSITIVE 510 67 21 89 41 aact:AA02474|3end_transmembrane_protein_1711548..1711698_POSITIVE 489 69 22 71 19 aact:AA02721|3end_spermidine/putrescine_ABC_transporter,_periplasmic-binding_protein_1905334..1905484_POSITIVE 477 63 22 79 38 aact:AA02781|3end_protein-export_membrane_protein_1945981..1946131_POSITIVE 461 65 21 53 8 aact:AA02992|3end_RNA_methyltransferase_2091740..2091890_POSITIVE 461 65 21 78 34 aact:AA02112|3end_cell_division_protein_1442184..1442334_POSITIVE 449 49 6 74 21 aact:AA00335|3end_PTS_fructose-specific_IIBC_component_228230..228380_POSITIVE 446 70 21 147 99 aact:AA02862|3end_hypothetical_protein_2008848..2008998_POSITIVE 434 65 22 84 41 aact:AA02360|3end_threonine_synthase_1619947..1620097_POSITIVE 433 64 22 85 43 aact:AA00120|3end_ATP-dependent_Clp_protease_ATP-binding_subunit_90281..90431_POSITIVE 429 58 15 145 98 aact:AA00623|3end_hypothetical_protein_430632..430782_POSITIVE 428 65 22 81 37 aact:AA01083|3end_preprotein_translocase_SecA_subunit_750970..751120_POSITIVE 426 65 21 47 3 aact:AA02751|3end_L-lactate_permease_1928868..1929018_POSITIVE 425 69 22 63 18 aact:AA01619|3end_phospho-2-dehydro-3-deoxyheptonate_aldolase_(DAHP_synthase)_1095578..1095728_POSITIVE 424 70 22 96 40 aact:AA01645|3end_ferric_transport_ABC-related_ATP-binding_protein_1115184..1115334_POSITIVE 422 63 22 69 28 aact:AA01963|3end_dnaK_suppressor_protein_1329438..1329588_POSITIVE 421 60 15 145 102 aact:AA01068|3end_hypothetical_protein_739850..740000_POSITIVE 408 70 22 87 28 aact:AA00718|3end_ABC-related_ATP-binding_protein;_MRP_protein_494723..494873_POSITIVE 394 60 16 122 76 aact:AA00495|3end_hypothetical_protein_345139..345289_POSITIVE 389 63 22 83 37 aact:AA02777|3end_tRNA-guanine_transglycosylase_1942527..1942677_POSITIVE