Origin IGS: taagaagaaattcaataaactcttttgctagtacaagagatggtggtagagtagagtcgcttttttaaatttagttttgttgttgtgtaataaagagtgaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttttcactctttattacacaacaacaaaactaaatttaaaaaagcgactctactctaccaccatctcttgtactagcaaaagagtttattgaatttcttctta Mask Tandem Repeat Region ================================================ taagaagaaattcaataaactcttttgctagtacaagagatggtggtagagtagagtcgcttttttaaatttagttttgttgttgtgtaataaagagtgaaaa Find is-nt database================================================ Query_seq: AA01618:AA01619|AA01618:AA01619:lipoprotein releasing system transmembrane protein:phospho-2-dehydro-3-deoxyheptonate aldolase (DAHP synthase):->->:1094459..1094561 103 taagaagaaattcaataaactcttttgctagtacaagagatggtggtagagtagagtcgcttttttaaatttagttttgttgttgtgtaataaagagtgaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01618:AA01619|AA01618:AA01619:lipoprotein releasing system transmembrane protein:phospho-2-dehydro-3-deoxyheptonate aldolase (DAHP synthase):->->:1094459..1094561 103 taagaagaaattcaataaactcttttgctagtacaagagatggtggtagagtagagtcgcttttttaaatttagttttgttgttgtgtaataaagagtgaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01618:AA01619|AA01618:AA01619:lipoprotein releasing system transmembrane protein:phospho-2-dehydro-3-deoxyheptonate aldolase (DAHP synthase):->->:1094459..1094561 103 taagaagaaattcaataaactcttttgctagtacaagagatggtggtagagtagagtcgcttttttaaatttagttttgttgttgtgtaataaagagtgaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagaagaaattcaataaactcttttgctagtacaagagatggtggtagagtagagtcgcttttttaaatttagttttgttgttgtgtaataaagagtgaaaa Predict ORF larger than 30AA ================================================ taagaagaaattcaataaactcttttgctagtacaagagatggtggtagagtagagtcgcttttttaaatttagttttgttgttgtgtaataaagagtgaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagaagaaattcaataaactcttttgctagtacaagagatggtggtagagtagagtcgcttttttaaatttagttttgttgttgtgtaataaagagtgaaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01618:AA01619|AA01618:AA01619:lipoprotein releasing system transmembrane protein:phospho-2-dehydro-3-deoxyheptonate aldolase (DAHP synthase):->->:1094459..1094561 103 taagaagaaattcaataaactcttttgctagtacaagagatggtggtagagtagagtcgcttttttaaatttagttttgttgttgtgtaataaagagtgaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 103 Len: 103 Subject: aact_AA01618_AA01619|lipoprotein releasing system transmembrane protein:phospho-2-dehydro-3-deoxyheptonate aldolase (DAHP synthase)|POSITIVE:POSITIVE|[1094459,1094561]|103 HSP 1 e-value: 4.0E-52 bit: 204.0 Len: 103 Query Start:1 Query End:103 Subject Strand: POSITIVE Subject Start: 1 Subject End: 103 taagaagaaattcaataaactcttttgctagtacaagagatggtggtagagtagagtcgcttttttaaatttagttttgttgttgtgtaataaagagtgaaaa taagaagaaattcaataaactcttttgctagtacaagagatggtggtagagtagagtcgcttttttaaatttagttttgttgttgtgtaataaagagtgaaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGAAGAAAUUCAAUAAACUCUUUUGCUAGUACAAGAGAUGGUGGUAGAGUAGAGUCGCUUUUUUAAAUUUAGUUUUGUUGUUGUGUAAUAAAGAGUGAAAA ...(((....))).....(((((.(..(((..........)))..).)))))....(((((((((......(((........)))......)))))))))... (-18.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUCACUCUUUAUUACACAACAACAAAACUAAAUUUAAAAAAGCGACUCUACUCUACCACCAUCUCUUGUACUAGCAAAAGAGUUUAUUGAAUUUCUUCUUA ...............................((((((((......((((((...(((..((........))..)))....))))))..))))))))....... ( -6.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 271 60 23 169 121 aact:AA00397|5end_Fe-S_oxidoreductase;_MiaB-like_RNA_modifying_enzyme_(2-methylthioadenine_synthetase)_266069..266299_POSITIVE 267 55 11 79 35 aact:AA01533|5end_conserved_hypothetical_protein_1028931..1029161_POSITIVE 267 62 34 92 58 aact:AA00552|5end_periplasmic_nitrate_reductase_precursor_377331..377561_POSITIVE 263 59 25 51 16 aact:AA02560|5end_integral_membrane_protein_1781412..1781642_POSITIVE 261 58 13 142 95 aact:AA01471|5end_type_I_restriction_enzyme_M_(modification_chain)_987321..987551_POSITIVE 258 72 33 180 137 aact:AA00287|5end_glutamate_5-kinase_(gamma-glutamyl_kinase)_200158..200388_POSITIVE 243 63 21 110 50 aact:AA01902|5end_conserved_hypothetical_protein_1282997..1283227_POSITIVE 241 87 42 195 150 aact:AA01191|5end_AMP-binding_enzyme_816000..816230_POSITIVE 240 87 42 92 43 aact:AA02714|5end_paraquat-inducible_protein_PqiA_homolog_1895807..1896037_POSITIVE 238 67 30 90 49 aact:AA02157|5end_TonB-dependent_outer_membrane_receptor_1475251..1475481_POSITIVE 238 52 22 228 195 aact:AA01159|5end_phosphatase_792828..793058_POSITIVE 238 85 43 182 138 aact:AA00949|5end_hypothetical_protein_656220..656450_POSITIVE 237 66 21 100 48 aact:AA02391|5end_hypothetical_protein_1640525..1640755_POSITIVE 237 63 27 152 117 aact:AA00849|5end_MraZ_cell_division_protein_586038..586268_POSITIVE 234 88 48 114 69 aact:AA02443|5end_cytochrome_C_related_protein_1686202..1686432_POSITIVE 234 88 48 87 42 aact:AA02444|5end_hypothetical_protein_1686175..1686405_POSITIVE 229 60 20 65 27 aact:AA02487|5end_hypothetical_protein_1726277..1726507_POSITIVE 229 60 17 181 145 aact:AA02484|5end_ABC_transporter,_ATP-binding_protein_1721077..1721307_POSITIVE 229 58 21 227 181 aact:AA01927|5end_conserved_hypothetical_protein_1300928..1301158_POSITIVE 229 60 17 192 152 aact:AA00895|5end_hypothetical_protein_621285..621515_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 267 62 34 84 50 aact:AA00551|3end_hypothetical_protein_377403..377553_POSITIVE 263 59 25 39 4 aact:AA02558|3end_conserved_hypothetical_protein_1781480..1781630_POSITIVE 262 52 20 104 64 aact:AA01777|3end_conserved_hypothetical_protein_1198341..1198491_POSITIVE 238 67 30 85 44 aact:AA02156|3end_conserved_hypothetical_protein_1475326..1475476_POSITIVE 237 66 21 59 7 aact:AA02390|3end_mannonate_dehydratase_1640564..1640714_POSITIVE 237 63 27 39 4 aact:AA00847|3end_S-adenosyl-dependent_methyltransferase_586005..586155_POSITIVE 234 88 48 104 59 aact:AA02442|3end_thioredoxin-family_protein_1686272..1686422_POSITIVE 229 60 17 85 49 aact:AA02483|3end_hypothetical_protein_1721061..1721211_POSITIVE 228 80 34 144 97 aact:AA02965|3end_hypothetical_protein_2074286..2074436_POSITIVE 228 66 26 44 7 aact:AA02422|3end_conserved_hypothetical_protein_1668426..1668576_POSITIVE 228 66 26 44 7 aact:AA02298|3end_conserved_hypothetical_protein_1571000..1571150_POSITIVE 228 66 26 44 7 aact:AA00622|3end_conserved_hypothetical_protein_430104..430254_POSITIVE 228 66 26 44 7 aact:AA00413|3end_conserved_hypothetical_protein_280554..280704_POSITIVE 224 87 41 95 45 aact:AA01458|3end_aminotransferase_980093..980243_POSITIVE 222 61 40 90 64 aact:AA02465|3end_glucosamine-6-phosphate_isomerase_1704885..1705035_POSITIVE 221 68 37 36 7 aact:AA02922|3end_gamma-glutamylphosphate_reductase_2047441..2047591_POSITIVE 216 67 21 113 69 aact:AA02826|3end_conserved_hypothetical_protein_1987108..1987258_POSITIVE 216 64 22 138 99 aact:AA02777|3end_tRNA-guanine_transglycosylase_1942527..1942677_POSITIVE 216 59 19 89 48 aact:AA01646|3end_hypothetical_protein_1115262..1115412_POSITIVE 214 64 21 150 99 aact:AA02172|3end_conserved_hypothetical_protein_1486650..1486800_POSITIVE