Origin IGS: taggctcccgagtgaaaagtgcggtggtttttctcagcgttttttagatcgggatcacacaaatcacaaaaatcctgccattcccttgtttttccttaaaggcctctttaaagttatgaaccgcattcagaatttattacgcataaaaggagtttttg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 caaaaactccttttatgcgtaataaattctgaatgcggttcataactttaaagaggcctttaaggaaaaacaagggaatggcaggatttttgtgatttgtgtgatcccgatctaaaaaacgctgagaaaaaccaccgcacttttcactcgggagccta Mask Tandem Repeat Region ================================================ taggctcccgagtgaaaagtgcggtggtttttctcagcgttttttagatcgggatcacacaaatcacaaaaatcctgccattcccttgtttttccttaaaggcctctttaaagttatgaaccgcattcagaatttattacgcataaaaggagtttttg Find is-nt database================================================ Query_seq: AA01611:AA01614|AA01611:AA01614:anthranilate synthase component II:D-isomer specific 2-hydroxyacid dehydrogenase family protein:->->:1089680..1089837 158 taggctcccgagtgaaaagtgcggtggtttttctcagcgttttttagatcgggatcacacaaatcacaaaaatcctgccattcccttgtttttccttaaaggcctctttaaagttatgaaccgcattcagaatttattacgcataaaaggagtttttg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01611:AA01614|AA01611:AA01614:anthranilate synthase component II:D-isomer specific 2-hydroxyacid dehydrogenase family protein:->->:1089680..1089837 158 taggctcccgagtgaaaagtgcggtggtttttctcagcgttttttagatcgggatcacacaaatcacaaaaatcctgccattcccttgtttttccttaaaggcctctttaaagttatgaaccgcattcagaatttattacgcataaaaggagtttttg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01611:AA01614|AA01611:AA01614:anthranilate synthase component II:D-isomer specific 2-hydroxyacid dehydrogenase family protein:->->:1089680..1089837 158 taggctcccgagtgaaaagtgcggtggtttttctcagcgttttttagatcgggatcacacaaatcacaaaaatcctgccattcccttgtttttccttaaaggcctctttaaagttatgaaccgcattcagaatttattacgcataaaaggagtttttg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taggctcccgagtgaaaagtgcggtggtttttctcagcgttttttagatcgggatcacacaaatcacaaaaatcctgccattcccttgtttttccttaaaggcctctttaaagttatgaaccgcattcagaatttattacgcataaaaggagtttttg Predict ORF larger than 30AA ================================================ taggctcccgagtgaaaagtgcggtggtttttctcagcgttttttagatcgggatcacacaaatcacaaaaatcctgccattcccttgtttttccttaaaggcctctttaaagttatgaaccgcattcagaatttattacgcataaaaggagtttttg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taggctcccgagtgaaaagtgcggtggtttttctcagcgttttttagatcgggatcacacaaatcacaaaaatcctgccattcccttgtttttccttaaaggcctctttaaagttatgaaccgcattcagaatttattacgcataaaaggagtttttg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01611:AA01614|AA01611:AA01614:anthranilate synthase component II:D-isomer specific 2-hydroxyacid dehydrogenase family protein:->->:1089680..1089837 158 taggctcccgagtgaaaagtgcggtggtttttctcagcgttttttagatcgggatcacacaaatcacaaaaatcctgccattcccttgtttttccttaaaggcctctttaaagttatgaaccgcattcagaatttattacgcataaaaggagtttttg Intra-Species Hit: Count: 1 Min: 1 Max: 158 Len: 158 Subject: aact_AA01611_AA01614|anthranilate synthase component II:D-isomer specific 2-hydroxyacid dehydrogenase family protein|POSITIVE:POSITIVE|[1089680,1089837]|158 HSP 1 e-value: 9.0E-85 bit: 313.0 Len: 158 Query Start:1 Query End:158 Subject Strand: POSITIVE Subject Start: 1 Subject End: 158 taggctcccgagtgaaaagtgcggtggtttttctcagcgttttttagatcgggatcacacaaatcacaaaaatcctgccattcccttgtttttccttaaaggcctctttaaagttatgaaccgcattcagaatttattacgcataaaaggagtttttg taggctcccgagtgaaaagtgcggtggtttttctcagcgttttttagatcgggatcacacaaatcacaaaaatcctgccattcccttgtttttccttaaaggcctctttaaagttatgaaccgcattcagaatttattacgcataaaaggagtttttg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGCUCCCGAGUGAAAAGUGCGGUGGUUUUUCUCAGCGUUUUUUAGAUCGGGAUCACACAAAUCACAAAAAUCCUGCCAUUCCCUUGUUUUUCCUUAAAGGCCUCUUUAAAGUUAUGAACCGCAUUCAGAAUUUAUUACGCAUAAAAGGAGUUUUUG ..((.((((((.((((((((((.(.((....)).).))))))))))..))))))))..........((((((((((.......((((...........))))........(((((.((((.....)))).)))))............)))).)))))) (-30.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAAAAACUCCUUUUAUGCGUAAUAAAUUCUGAAUGCGGUUCAUAACUUUAAAGAGGCCUUUAAGGAAAAACAAGGGAAUGGCAGGAUUUUUGUGAUUUGUGUGAUCCCGAUCUAAAAAACGCUGAGAAAAACCACCGCACUUUUCACUCGGGAGCCUA ......((((((((((.....)))))...((((((((((......((((((((....))))))))........((((.(.(((.((.((....)).)).))).))))).........................))))))...))))...))))).... (-32.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 441 54 12 140 99 aact:AA02706|5end_2-oxoglutarate_dehydrogenase_E1_component_(decarboxylase_component)_1886843..1887073_POSITIVE 437 44 3 123 76 aact:AA00854|5end_arginine_transport_system_permease_protein_588898..589128_POSITIVE 432 60 12 155 109 aact:AA02700|5end_succinyl-CoA_synthase,_alpha_subunit_1883256..1883486_POSITIVE 422 60 14 91 52 aact:AA00264|5end_hypothetical_protein_185052..185282_POSITIVE 417 43 3 209 160 aact:AA01284|5end_heat_shock_protein_(hsp60)_868574..868804_POSITIVE 410 44 2 122 87 aact:AA01463|5end_probable_transport_protein_982732..982962_POSITIVE 410 47 13 175 141 aact:AA00084|5end_aminotransferase_60814..61044_POSITIVE 396 60 15 137 93 aact:AA02673|5end_gluconate_utilization_operon_repressor_1858913..1859143_POSITIVE 396 58 12 157 97 aact:AA00250|5end_lipoate_biosynthesis_protein_A_175496..175726_POSITIVE 393 51 14 141 103 aact:AA00789|5end_DNA_topoisomerase_III_540486..540716_POSITIVE 390 44 2 86 36 aact:AA00906|5end_sigma-E_factor_regulatory_protein_625110..625340_POSITIVE 390 53 11 106 63 aact:AA00065|5end_hypothetical_protein_48854..49084_POSITIVE 377 53 11 167 123 aact:AA00557|5end_nitrate/nitrite_sensor_protein_380408..380638_POSITIVE 372 44 8 206 174 aact:AA01883|5end_recycling_protein;_permease_1269821..1270051_POSITIVE 372 48 14 157 124 aact:AA01824|5end_iron(III)_ABC_transporter,_ATP-binding_protein_1227705..1227935_POSITIVE 370 43 9 139 104 aact:AA02597|5end_ribonuclease_D_1807124..1807354_POSITIVE 369 44 6 131 85 aact:AA01918|5end_anaerobic_C4-dicarboxylate_transporter_1290931..1291161_POSITIVE 364 44 11 168 134 aact:AA02381|5end_4-hydroxy-2-oxoglutarate_aldolase_1629192..1629422_POSITIVE 364 60 11 91 33 aact:AA02143|5end_hypothetical_protein_1467433..1467663_POSITIVE 362 47 8 124 87 aact:AA02306|5end_DNA-directed_RNA_polymerase,_omega_chain_1576947..1577177_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 441 54 12 145 104 aact:AA02705|3end_hypothetical_protein_1886928..1887078_POSITIVE 422 60 14 54 15 aact:AA00263|3end_ATP-dependent_RNA_helicase_185095..185245_POSITIVE 417 43 3 85 36 aact:AA01280|3end_groES_chaperonin_868530..868680_POSITIVE 410 44 2 89 54 aact:AA01462|3end_xylose_operon_regulatory_protein_982779..982929_POSITIVE 410 47 13 132 98 aact:AA00083|3end_hypothetical_protein_60851..61001_POSITIVE 410 47 13 48 14 aact:AA00081|3end_fatty_acid_metabolism_regulator_protein_60767..60917_POSITIVE 409 44 8 89 57 aact:AA00523|3end_conserved_hypothetical_protein_358929..359079_POSITIVE 396 60 15 61 17 aact:AA02671|3end_gluconokinase_1858917..1859067_POSITIVE 390 60 16 123 73 aact:AA02253|3end_hypothetical_protein_1534786..1534936_POSITIVE 390 53 11 118 75 aact:AA00064|3end_ribosomal_small_subunit_pseudouridine_synthase_48946..49096_POSITIVE 382 59 12 99 55 aact:AA02210|3end_ribonucleoside-diphosphate_reductase_alpha_chain_1505956..1506106_POSITIVE 377 53 11 121 77 aact:AA00555|3end_hypothetical_protein_380442..380592_POSITIVE 372 44 8 70 38 aact:AA01881|3end_adenylate_kinase_1269765..1269915_POSITIVE 372 48 14 119 86 aact:AA01823|3end_hypothetical_protein_1227747..1227897_POSITIVE 372 48 14 43 10 aact:AA01822|3end_lysostaphin_precursor_1227671..1227821_POSITIVE 370 43 9 45 10 aact:AA02595|3end_cytochrome_c_peroxidase_1807110..1807260_POSITIVE 369 44 6 116 70 aact:AA01917|3end_hypothetical_protein_1290996..1291146_POSITIVE 364 44 11 76 42 aact:AA02380|3end_iron_chelatin_ABC_transporter,periplasmic-binding_protein_1629180..1629330_POSITIVE 364 60 11 110 52 aact:AA02142|3end_outer_membrane_protein_OprM_precursor_1467532..1467682_POSITIVE 362 47 8 75 38 aact:AA02305|3end_guanosine-3',5'-bis(diphosphate)3'-_pyrophosphohydrolase_1576978..1577128_POSITIVE