Origin IGS: taattaagcagccataattttcctccttgttgctataagcttttggatggcgggttatggcttttattctatttagtgagtgattgatttttgttcagtttaataagaaggttact .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agtaaccttcttattaaactgaacaaaaatcaatcactcactaaatagaataaaagccataacccgccatccaaaagcttatagcaacaaggaggaaaattatggctgcttaatta Mask Tandem Repeat Region ================================================ taattaagcagccataattttcctccttgttgctataagcttttggatggcgggttatggcttttattctatttagtgagtgattgatttttgttcagtttaataagaaggttact Find is-nt database================================================ Query_seq: AA01599:AA01600|AA01599:AA01600:phosphocarrier protein HPr:phosphotransferase system enzyme I:->->:1080505..1080620 116 taattaagcagccataattttcctccttgttgctataagcttttggatggcgggttatggcttttattctatttagtgagtgattgatttttgttcagtttaataagaaggttact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01599:AA01600|AA01599:AA01600:phosphocarrier protein HPr:phosphotransferase system enzyme I:->->:1080505..1080620 116 taattaagcagccataattttcctccttgttgctataagcttttggatggcgggttatggcttttattctatttagtgagtgattgatttttgttcagtttaataagaaggttact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01599:AA01600|AA01599:AA01600:phosphocarrier protein HPr:phosphotransferase system enzyme I:->->:1080505..1080620 116 taattaagcagccataattttcctccttgttgctataagcttttggatggcgggttatggcttttattctatttagtgagtgattgatttttgttcagtttaataagaaggttact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taattaagcagccataattttcctccttgttgctataagcttttggatggcgggttatggcttttattctatttagtgagtgattgatttttgttcagtttaataagaaggttact Predict ORF larger than 30AA ================================================ taattaagcagccataattttcctccttgttgctataagcttttggatggcgggttatggcttttattctatttagtgagtgattgatttttgttcagtttaataagaaggttact Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taattaagcagccataattttcctccttgttgctataagcttttggatggcgggttatggcttttattctatttagtgagtgattgatttttgttcagtttaataagaaggttact Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01599:AA01600|AA01599:AA01600:phosphocarrier protein HPr:phosphotransferase system enzyme I:->->:1080505..1080620 116 taattaagcagccataattttcctccttgttgctataagcttttggatggcgggttatggcttttattctatttagtgagtgattgatttttgttcagtttaataagaaggttact Intra-Species Hit: Count: 1 Min: 1 Max: 116 Len: 116 Subject: aact_AA01599_AA01600|phosphocarrier protein HPr:phosphotransferase system enzyme I|POSITIVE:POSITIVE|[1080505,1080620]|116 HSP 1 e-value: 8.0E-60 bit: 230.0 Len: 116 Query Start:1 Query End:116 Subject Strand: POSITIVE Subject Start: 1 Subject End: 116 taattaagcagccataattttcctccttgttgctataagcttttggatggcgggttatggcttttattctatttagtgagtgattgatttttgttcagtttaataagaaggttact taattaagcagccataattttcctccttgttgctataagcttttggatggcgggttatggcttttattctatttagtgagtgattgatttttgttcagtttaataagaaggttact Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUAAGCAGCCAUAAUUUUCCUCCUUGUUGCUAUAAGCUUUUGGAUGGCGGGUUAUGGCUUUUAUUCUAUUUAGUGAGUGAUUGAUUUUUGUUCAGUUUAAUAAGAAGGUUACU ..(((((((.(((((((((..(((((.....((.....))....))).))..)))))))))..((((((........))))))..............)))))))............ (-28.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUAACCUUCUUAUUAAACUGAACAAAAAUCAAUCACUCACUAAAUAGAAUAAAAGCCAUAACCCGCCAUCCAAAAGCUUAUAGCAACAAGGAGGAAAAUUAUGGCUGCUUAAUUA ..................................................(((.((((((((....((.(((....((.....)).....)))))....))))))))..))).... (-16.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 326 62 26 60 21 aact:AA01192|5end_conserved_hypothetical_protein_(possible_membrane_protein)_817358..817588_POSITIVE 323 56 12 137 88 aact:AA01533|5end_conserved_hypothetical_protein_1028931..1029161_POSITIVE 307 70 21 104 60 aact:AA02140|5end_ABC_transporter_ATP-binding_protein_1462144..1462374_POSITIVE 303 61 21 75 19 aact:AA01649|5end_maltose_ABC_transporter,_permease_protein_1117316..1117546_POSITIVE 298 68 27 183 143 aact:AA02175|5end_anthranilate_synthase_component_II_1488299..1488529_POSITIVE 293 62 25 228 198 aact:AA01476|5end_hypothetical_protein_991402..991632_POSITIVE 291 78 39 177 137 aact:AA02357|5end_bifunctional_protein:_aspartokinase_I;_homoserine_dehydrogenaseI_1614668..1614898_POSITIVE 290 80 31 88 24 aact:AA02050|5end_1-deoxy-D-xylulose_5-phosphate_synthase_1395625..1395855_POSITIVE 288 61 23 148 98 aact:AA01127|5end_energy_transducer_protein_772685..772915_POSITIVE 285 69 21 189 133 aact:AA01668|5end_transcription_elongation_factor_1129830..1130060_POSITIVE 284 68 27 65 30 aact:AA00408|5end_SUN_protein_(FMU_protein)_272256..272486_POSITIVE 276 61 24 151 118 aact:AA02206|5end_tryptophan_synthase,_beta_chain_1501917..1502147_POSITIVE 273 61 24 84 45 aact:AA01473|5end_type_I_restriction-modification_system_S_(specificity_subunit)_989025..989255_POSITIVE 273 50 10 158 119 aact:AA01224|5end_CTP_synthase_836221..836451_POSITIVE 268 80 32 113 69 aact:AA00810|5end_alcohol_dehydrogenase_555626..555856_POSITIVE 266 80 38 147 106 aact:AA01287|5end_transposase_871583..871813_POSITIVE 264 98 51 217 158 aact:AA00871|5end_rough_colony_protein_A_602561..602791_POSITIVE 263 50 6 130 82 aact:AA01391|5end_glycerol-3-phosphate_acyltransferase_937887..938117_POSITIVE 262 60 15 187 136 aact:AA01393|5end_ABC_transporter,_ATP-binding_protein_940369..940599_POSITIVE 261 65 26 189 141 aact:AA00333|5end_1-phosphofructokinase;_tagatose-6-phosphate_kinase_225543..225773_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 326 62 26 60 21 aact:AA01191|3end_AMP-binding_enzyme_817438..817588_POSITIVE 323 56 12 72 23 aact:AA01532|3end_hypothetical_protein_1028946..1029096_POSITIVE 307 70 21 98 54 aact:AA02139|3end_possible_membrane_protein_1462218..1462368_POSITIVE 305 61 27 149 114 aact:AA01224|3end_CTP_synthase_837899..838049_POSITIVE 291 78 39 85 45 aact:AA02356|3end_hypothetical_protein_1614656..1614806_POSITIVE 290 80 31 78 14 aact:AA02049|3end_hypothetical_protein_1395695..1395845_POSITIVE 288 69 25 151 104 aact:AA01709|3end_glutathione-S-transferase-nitroreductase_A_fusion_protein;_modulator_of_drug_activity_A_1159334..1159484_POSITIVE 288 61 23 135 85 aact:AA01124.1|3end_biopolymer_transport_protein_D_772752..772902_POSITIVE 288 61 23 134 84 aact:AA01125|3end_hypothetical_protein_772751..772901_POSITIVE 284 68 27 38 3 aact:AA00407|3end_trk_system_potassium_uptake_protein_272309..272459_POSITIVE 278 53 12 63 9 aact:AA00540|3end_hypothetical_protein_370261..370411_POSITIVE 276 97 51 100 39 aact:AA02972|3end_conserved_hypothetical_protein_(tyrosine_phenol_lyase-related_protein)_2077622..2077772_POSITIVE 276 61 24 138 105 aact:AA02205|3end_short_chain_dehydrogenase/reductase_1501984..1502134_POSITIVE 274 70 21 109 32 aact:AA01404|3end_hypothetical_protein_948834..948984_POSITIVE 273 61 24 137 98 aact:AA01472|3end_hypothetical_protein_989158..989308_POSITIVE 271 54 24 144 110 aact:AA01607|3end_oligopeptide_transporter,_periplasmic-binding_protein_1087112..1087262_POSITIVE 262 60 15 140 89 aact:AA01391|3end_glycerol-3-phosphate_acyltransferase_940402..940552_POSITIVE 260 50 10 115 66 aact:AA01455|3end_D-xylose_ABC_transporter,_periplasmic-binding_protein_976106..976256_POSITIVE 260 63 23 51 18 aact:AA00271|3end_hypothetical_protein_190469..190619_POSITIVE 260 66 26 146 111 aact:AA00106|3end_1-hydroxy-2-methyl-2-(E)-butenyl_4-diphosphate_synthase_76724..76874_POSITIVE