Origin IGS: tgagatggtttactgctttttaggcattgaatgttaatttttggttaaaagattaatttattttgaatttttttgtgatctgattcaaacttttgggtaaaacggcttgcatcagaagatgaaacacaagatactgtcatttattttagtattttaaataaataattgaaaaaatactgaaatgaattaaatgcattctattaactttttagaggaaagtaac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gttactttcctctaaaaagttaatagaatgcatttaattcatttcagtattttttcaattatttatttaaaatactaaaataaatgacagtatcttgtgtttcatcttctgatgcaagccgttttacccaaaagtttgaatcagatcacaaaaaaattcaaaataaattaatcttttaaccaaaaattaacattcaatgcctaaaaagcagtaaaccatctca Mask Tandem Repeat Region ================================================ tgagatggtttactgctttttaggcattgaatgttaatttttggttaaaagattaatttattttgaatttttttgtgatctgattcaaacttttgggtaaaacggcttgcatcagaagatgaaacacaagatactgtcatttattttagtattttaaataaataattgaaaaaatactgaaatgaattaaatgcattctattaactttttagaggaaagtaac Find is-nt database================================================ Query_seq: AA01598:AA01599|AA01598:AA01599:conserved hypothetical protein:phosphocarrier protein HPr:->->:1080027..1080249 223 tgagatggtttactgctttttaggcattgaatgttaatttttggttaaaagattaatttattttgaatttttttgtgatctgattcaaacttttgggtaaaacggcttgcatcagaagatgaaacacaagatactgtcatttattttagtattttaaataaataattgaaaaaatactgaaatgaattaaatgcattctattaactttttagaggaaagtaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01598:AA01599|AA01598:AA01599:conserved hypothetical protein:phosphocarrier protein HPr:->->:1080027..1080249 223 tgagatggtttactgctttttaggcattgaatgttaatttttggttaaaagattaatttattttgaatttttttgtgatctgattcaaacttttgggtaaaacggcttgcatcagaagatgaaacacaagatactgtcatttattttagtattttaaataaataattgaaaaaatactgaaatgaattaaatgcattctattaactttttagaggaaagtaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01598:AA01599|AA01598:AA01599:conserved hypothetical protein:phosphocarrier protein HPr:->->:1080027..1080249 223 tgagatggtttactgctttttaggcattgaatgttaatttttggttaaaagattaatttattttgaatttttttgtgatctgattcaaacttttgggtaaaacggcttgcatcagaagatgaaacacaagatactgtcatttattttagtattttaaataaataattgaaaaaatactgaaatgaattaaatgcattctattaactttttagaggaaagtaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgagatggtttactgctttttaggcattgaatgttaatttttggttaaaagattaatttattttgaatttttttgtgatctgattcaaacttttgggtaaaacggcttgcatcagaagatgaaacacaagatactgtcatttattttagtattttaaataaataattgaaaaaatactgaaatgaattaaatgcattctattaactttttagaggaaagtaac Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: - Start: 48 End: 140 ............................................... F I L K N Q I K K H D S E F K Q T F R S A D S S S V C S V T M ................................................................................... tgagatggtttactgctttttaggcattgaatgttaatttttggttaaaagattaatttattttgaatttttttgtgatctgattcaaacttttgggtaaaacggcttgcatcagaagatgaaacacaagatactgtcatttattttagtattttaaataaataattgaaaaaatactgaaatgaattaaatgcattctattaactttttagaggaaagtaac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgagatggtttactgctttttaggcattgaatgttaatttttggttaaaagattaatttattttgaatttttttgtgatctgattcaaacttttgggtaaaacggcttgcatcagaagatgaaacacaagatactgtcatttattttagtattttaaataaataattgaaaaaatactgaaatgaattaaatgcattctattaactttttagaggaaagtaac Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01598:AA01599|AA01598:AA01599:conserved hypothetical protein:phosphocarrier protein HPr:->->:1080027..1080249 223 tgagatggtttactgctttttaggcattgaatgttaatttttggttaaaagattaatttattttgaatttttttgtgatctgattcaaacttttgggtaaaacggcttgcatcagaagatgaaacacaagatactgtcatttattttagtattttaaataaataattgaaaaaatactgaaatgaattaaatgcattctattaactttttagaggaaagtaac Intra-Species Hit: Count: 1 Min: 75 Max: 223 Len: 149 Subject: aact_AA01598_AA01599|conserved hypothetical protein:phosphocarrier protein HPr|POSITIVE:POSITIVE|[1080027,1080249]|223 HSP 1 e-value: 3.0E-79 bit: 295.0 Len: 149 Query Start:75 Query End:223 Subject Strand: POSITIVE Subject Start: 75 Subject End: 223 ..........................................................................gtgatctgattcaaacttttgggtaaaacggcttgcatcagaagatgaaacacaagatactgtcatttattttagtattttaaataaataattgaaaaaatactgaaatgaattaaatgcattctattaactttttagaggaaagtaac ..........................................................................gtgatctgattcaaacttttgggtaaaacggcttgcatcagaagatgaaacacaagatactgtcatttattttagtattttaaataaataattgaaaaaatactgaaatgaattaaatgcattctattaactttttagaggaaagtaac Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUGAUCUGAUUCAAACUUUUGGGUAAAACGGCUUGCAUCAGAAGAUGAAACACAAGAUACUGUCAUUUAUUUUAGUAUUUUAAAUAAAUAAUUGAAAAAAUACUGAAAUGAAUUAAAUGCAUUCUAUUAACUUUUUAGAGGAAAGUAAC (((.(((..((((..(((((((((((......)))).))))))).)))).....))))))....(((((((((((((((((.(((.....)))...)))))))))))))))))....(((.((((.((((....)))).)))).))).. (-27.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUACUUUCCUCUAAAAAGUUAAUAGAAUGCAUUUAAUUCAUUUCAGUAUUUUUUCAAUUAUUUAUUUAAAAUACUAAAAUAAAUGACAGUAUCUUGUGUUUCAUCUUCUGAUGCAAGCCGUUUUACCCAAAAGUUUGAAUCAGAUCAC ...(((((.......))))).....(((((((..((..((((((.((((((((...............)))))))).....))))))...))...))))))).....((((((.(((((..((......))..))))).)))))).... (-19.96) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 293 46 2 166 125 aact:AA02100|5end_lipoprotein_1430074..1430304_POSITIVE 271 36 1 193 158 aact:AA02431|5end_conserved_hypothetical_protein_1675594..1675824_POSITIVE 253 67 22 92 46 aact:AA01780|5end_ABC-related_molybdenum_transport_system_permease_protein_1201084..1201314_POSITIVE 245 48 1 62 1 aact:AA01344|5end_magnesium_and_cobalt_transport_protein_911661..911891_POSITIVE 242 65 29 63 25 aact:AA01886|5end_UDP-glucose-4-epimerase_1271464..1271694_POSITIVE 237 45 1 220 180 aact:AA02367|5end_conserved_hypothetical_protein_1623174..1623404_POSITIVE 237 45 1 66 26 aact:AA02366|5end_hypothetical_protein_1623020..1623250_POSITIVE 234 48 1 228 186 aact:AA01432|5end_lysophospholipase_L2_960715..960945_POSITIVE 234 43 5 230 183 aact:AA00763|5end_hemoglobin_binding_protein_A_524976..525206_POSITIVE 229 63 21 111 57 aact:AA00443|5end_hypothetical_protein_307601..307831_POSITIVE 226 47 2 218 174 aact:AA01877|5end_conserved_hypothetical_protein_1264115..1264345_POSITIVE 224 50 1 99 47 aact:AA02716|5end_peptidase_T_1899789..1900019_POSITIVE 224 48 6 205 168 aact:AA00287|5end_glutamate_5-kinase_(gamma-glutamyl_kinase)_200158..200388_POSITIVE 223 47 4 136 103 aact:AA00145|5end_probable_degenerate_transposase_fragment_105046..105276_POSITIVE 221 38 2 173 131 aact:AA01855|5end_transcriptional_regulator,_Cro/CI_family_1247009..1247239_POSITIVE 219 70 22 78 22 aact:AA00952|5end_hemY_protein_homolog_657541..657771_POSITIVE 216 47 19 52 14 aact:AA00601|5end_hypothetical_protein_408863..409093_POSITIVE 215 59 17 223 174 aact:AA02851|5end_hypothetical_protein_2000896..2001126_POSITIVE 215 68 21 175 128 aact:AA00981|5end_carboxylesterase_685245..685475_POSITIVE 214 69 27 175 123 aact:AA02566|5end_PTS_system,_IIC_component_1787008..1787238_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 268 64 21 144 93 aact:AA02959|3end_pyrroline-5-carboxylate_(P5C)_reductase_2070976..2071126_POSITIVE 253 67 22 102 56 aact:AA01779|3end_molybdenum_ABC_transporter,_ATP-binding_protein_1201174..1201324_POSITIVE 242 65 29 54 16 aact:AA01885|3end_hydrogenase_nickel_incorporation_protein_1271535..1271685_POSITIVE 238 75 31 63 26 aact:AA00882|3end_hypothetical_protein_613645..613795_POSITIVE 229 63 21 111 57 aact:AA00442|3end_conserved_hypothetical_protein_(possible_zinc_finger_protein)_307681..307831_POSITIVE 227 40 1 44 11 aact:AA02545|3end_conserved_hypothetical_protein_1774221..1774371_POSITIVE 216 49 1 62 19 aact:AA02835|3end_hypothetical_protein_1991876..1992026_POSITIVE 216 47 19 48 10 aact:AA00600|3end_ribosome_recycling_factor_(ribosome_releasing_factor)_408939..409089_POSITIVE 213 44 1 58 10 aact:AA00253|3end_hypothetical_protein_177636..177786_POSITIVE 212 49 4 86 44 aact:AA00279|3end_8-amino-7-oxonoanoate_synthase_196514..196664_POSITIVE 210 37 2 135 107 aact:AA00046|3end_formate_dependent_nitrite_reductase,_cytochrome-c_type_protein_36756..36906_POSITIVE 207 48 1 149 106 aact:AA02917|3end_cystathionine_gamma-synthase_2043280..2043430_POSITIVE 206 42 1 87 46 aact:AA02893|3end_oligopeptide_transport_system_permease_protein,_N-terminal_2027544..2027694_POSITIVE 206 40 2 148 110 aact:AA01876|3end_PTS_system,_IIBC_components_1264131..1264281_POSITIVE 204 48 1 100 54 aact:AA00408|3end_SUN_protein_(FMU_protein)_273688..273838_POSITIVE 203 52 11 38 1 aact:AA02484|3end_ABC_transporter,_ATP-binding_protein_1722920..1723070_POSITIVE 203 60 16 104 40 aact:AA01784|3end_sodium-dependent_transporter_1205299..1205449_POSITIVE 202 62 29 143 105 aact:AA02145|3end_iron(III)_ABC_transporter,_ATP-binding_protein_1469176..1469326_POSITIVE 202 35 6 130 100 aact:AA00921|3end_conserved_hypothetical_protein_634014..634164_POSITIVE 202 50 18 58 10 aact:AA00038|3end_conserved_hypothetical_protein_30392..30542_POSITIVE