Origin IGS: tatgcctcctgatgtgaataaattttacaaaaatagaaaatgcttataaaaaagtgcggtcggatttcccggcgtttttaaaataccgaaaattcccaccgcacttttgctcaagacatgactaacgaaccatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatggttcgttagtcatgtcttgagcaaaagtgcggtgggaattttcggtattttaaaaacgccgggaaatccgaccgcacttttttataagcattttctatttttgtaaaatttattcacatcaggaggcata Mask Tandem Repeat Region ================================================ tatgcctcctgatgtgaataaattttacaaaaatagaaaatgcttataaaaaagtgcggtcggatttcccggcgtttttaaaataccgaaaattcccaccgcacttttgctcaagacatgactaacgaaccatta Find is-nt database================================================ Query_seq: AA01563:AA01564|AA01563:AA01564:transketolase isozyme 1:transaldolase B:->->:1049104..1049238 135 tatgcctcctgatgtgaataaattttacaaaaatagaaaatgcttataaaaaagtgcggtcggatttcccggcgtttttaaaataccgaaaattcccaccgcacttttgctcaagacatgactaacgaaccatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01563:AA01564|AA01563:AA01564:transketolase isozyme 1:transaldolase B:->->:1049104..1049238 135 tatgcctcctgatgtgaataaattttacaaaaatagaaaatgcttataaaaaagtgcggtcggatttcccggcgtttttaaaataccgaaaattcccaccgcacttttgctcaagacatgactaacgaaccatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01563:AA01564|AA01563:AA01564:transketolase isozyme 1:transaldolase B:->->:1049104..1049238 135 tatgcctcctgatgtgaataaattttacaaaaatagaaaatgcttataaaaaagtgcggtcggatttcccggcgtttttaaaataccgaaaattcccaccgcacttttgctcaagacatgactaacgaaccatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tatgcctcctgatgtgaataaattttacaaaaatagaaaatgcttataaaaaagtgcggtcggatttcccggcgtttttaaaataccgaaaattcccaccgcacttttgctcaagacatgactaacgaaccatta Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 18 End: 122 ................. M N F T K I E N A Y K K V R S D F P A F L K Y R K F P P H F C S R H D ............. tatgcctcctgatgtgaataaattttacaaaaatagaaaatgcttataaaaaagtgcggtcggatttcccggcgtttttaaaataccgaaaattcccaccgcacttttgctcaagacatgactaacgaaccatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 82 Start: 22 End: 51 .....................TTTTATAAGCATTTTCTATTTTTGTAAAAT.................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tatgcctcctgatgtgaataaattttacaaaaatagaaaatgcttataaaaaagtgcggtcggatttcccggcgtttttaaaataccgaaaattcccaccgcacttttgctcaagacatgactaacgaaccatta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -14.8 Tail_Score: -5.25803 Start: 54 End: 104 Full_Region: atgtcttgagcaaaa gtgcggtgggaattttcggtattt taa aaacgccgggaaatccgaccgcac ttttttataagcatt .....................................................gtgcggtgggaattttcggtattttaaaaacgccgggaaatccgaccgcac............................... Find igs database================================================ Query_seq: AA01563:AA01564|AA01563:AA01564:transketolase isozyme 1:transaldolase B:->->:1049104..1049238 135 tatgcctcctgatgtgaataaattttacaaaaatagaaaatgcttataaaaaagtgcggtcggatttcccggcgtttttaaaataccgaaaattcccaccgcacttttgctcaagacatgactaacgaaccatta Intra-Species Hit: Count: 1 Min: 1 Max: 135 Len: 135 Subject: aact_AA01563_AA01564|transketolase isozyme 1:transaldolase B|POSITIVE:POSITIVE|[1049104,1049238]|135 HSP 1 e-value: 4.0E-71 bit: 268.0 Len: 135 Query Start:1 Query End:135 Subject Strand: POSITIVE Subject Start: 1 Subject End: 135 tatgcctcctgatgtgaataaattttacaaaaatagaaaatgcttataaaaaagtgcggtcggatttcccggcgtttttaaaataccgaaaattcccaccgcacttttgctcaagacatgactaacgaaccatta tatgcctcctgatgtgaataaattttacaaaaatagaaaatgcttataaaaaagtgcggtcggatttcccggcgtttttaaaataccgaaaattcccaccgcacttttgctcaagacatgactaacgaaccatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUGCCUCCUGAUGUGAAUAAAUUUUACAAAAAUAGAAAAUGCUUAUAAAAAAGUGCGGUCGGAUUUCCCGGCGUUUUUAAAAUACCGAAAAUUCCCACCGCACUUUUGCUCAAGACAUGACUAACGAACCAUUA .(((..((....((((((.....))))))....(((...((((((....(((((((((((.(((.((..(((..((.....))..)))..)).))).)))))))))))....))).)))..)))..))..))).. (-34.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGGUUCGUUAGUCAUGUCUUGAGCAAAAGUGCGGUGGGAAUUUUCGGUAUUUUAAAAACGCCGGGAAAUCCGACCGCACUUUUUUAUAAGCAUUUUCUAUUUUUGUAAAAUUUAUUCACAUCAGGAGGCAUA .............(((...((((((...((((((((((.(((.((..(((..((.....))..)))..)).))).))))))))))((((((((.((.....)).))))))))...........)))))))))... (-41.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 460 87 48 57 20 aact:AA01877|5end_conserved_hypothetical_protein_1264115..1264345_POSITIVE 436 97 51 212 162 aact:AA02743|5end_outer_membrane_lipoprotein_precursor/glutamyl-tRNA_reductase_subunit_1922650..1922880_POSITIVE 436 92 51 149 109 aact:AA00819|5end_D-alanyl-D-alanine_carboxypeptidase_562644..562874_POSITIVE 434 100 51 149 99 aact:AA01059|5end_orotate_phosphoribosyltransferase_735052..735282_POSITIVE 433 99 51 120 70 aact:AA02199|5end_hypothetical_protein_1497627..1497857_POSITIVE 432 97 51 191 142 aact:AA00511|5end_nicotinamide_mononucleotide_transporter_353884..354114_POSITIVE 416 100 51 92 32 aact:AA00602|5end_1-deoxy-D-xylulose_5-phosphate_reductoisomerase_409077..409307_POSITIVE 404 100 51 161 112 aact:AA02597|5end_ribonuclease_D_1807124..1807354_POSITIVE 404 100 51 166 117 aact:AA01666|5end_penicillin-binding_protein_4;_D-alanyl-D-alanine_carboxypeptidase;_D-alanyl-D-alanine-endopeptidase_1128231..1128461_POSITIVE 396 100 51 198 149 aact:AA02177|5end_conserved_hypothetical_protein_1488963..1489193_POSITIVE 393 80 50 137 107 aact:AA00679|5end_cytoplasmic_axial_filament_(Ribonuclease_G)_463288..463518_POSITIVE 389 78 34 161 107 aact:AA00557|5end_nitrate/nitrite_sensor_protein_380408..380638_POSITIVE 388 121 81 126 83 aact:AA01008|5end_mazG_beta-lactamase_regulatory_protein_homolog_703470..703700_POSITIVE 387 89 50 57 17 aact:AA02484|5end_ABC_transporter,_ATP-binding_protein_1721077..1721307_POSITIVE 386 90 50 190 143 aact:AA03003|5end_GTP-binding_membrane_protein_2096544..2096774_POSITIVE 386 90 50 69 22 aact:AA03002|5end_hypothetical_protein_2096423..2096653_POSITIVE 379 79 41 226 190 aact:AA01598|5end_conserved_hypothetical_protein_1078954..1079184_POSITIVE 374 107 69 131 84 aact:AA01532|5end_hypothetical_protein_1028771..1029001_POSITIVE 374 107 69 65 18 aact:AA01531|5end_hypothetical_protein_1028705..1028935_POSITIVE 373 100 51 176 126 aact:AA01824|5end_iron(III)_ABC_transporter,_ATP-binding_protein_1227705..1227935_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 434 100 51 64 14 aact:AA01057|3end_DnaJ-like_protein_djlA_735047..735197_POSITIVE 433 99 51 105 55 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 432 97 51 110 61 aact:AA00508|3end_SAM-dependent_methyltransferase_353883..354033_POSITIVE 404 100 51 67 18 aact:AA02595|3end_cytochrome_c_peroxidase_1807110..1807260_POSITIVE 404 100 51 59 10 aact:AA01663|3end_GTP_cyclohydrolase_I_1128204..1128354_POSITIVE 401 99 53 58 1 aact:AA01945|3end_selenophosphate_synthase_1316022..1316172_POSITIVE 396 100 51 121 72 aact:AA02175|3end_anthranilate_synthase_component_II_1488966..1489116_POSITIVE 389 78 34 115 61 aact:AA00555|3end_hypothetical_protein_380442..380592_POSITIVE 386 90 50 53 6 aact:AA03001|3end_probable_glycyl_radical_protein/pyruvate-formate_lyase_2096487..2096637_POSITIVE 379 79 41 128 92 aact:AA01597|3end_oligoribonuclease_1078936..1079086_POSITIVE 373 100 51 138 88 aact:AA01823|3end_hypothetical_protein_1227747..1227897_POSITIVE 373 100 51 62 12 aact:AA01822|3end_lysostaphin_precursor_1227671..1227821_POSITIVE 363 88 44 80 35 aact:AA02280|3end_conserved_hypothetical_protein_1552767..1552917_POSITIVE 360 109 62 72 24 aact:AA01648|3end_alpha-amylase_protein;_1,4-alpha-D-glucan_glucanohydrolase_1117298..1117448_POSITIVE 356 100 51 97 48 aact:AA01751|3end_conserved_hypothetical_protein_1185392..1185542_POSITIVE 356 119 71 88 40 aact:AA01457|3end_D-xylose_ABC_transporter,_membrane_spanning_protein_978808..978958_POSITIVE 354 129 81 55 4 aact:AA00950|3end_conserved_hypothetical_protein_657574..657724_POSITIVE 354 80 35 80 31 aact:AA00251|3end_lipoate_biosynthesis_protein_B_177238..177388_POSITIVE 352 90 41 59 10 aact:AA02462|3end_thiol_peroxidase_(scavengase)_1702673..1702823_POSITIVE 352 100 51 147 88 aact:AA01917|3end_hypothetical_protein_1290996..1291146_POSITIVE