Origin IGS: aaaaatctgcatcttattaactagttgggtgtagtgtccaacttttgggtgcagatcatttttttagttaaaaaattatctaattcatcaaacaaatataatgcccctctaggaaaacccttagaagcaaaggctctatttctcattgattgcgtatattccatttgttcaattctcaatctaatcatttatcaaatagtaaaacactaccactactaaaatcgaccgcacttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaagtgcggtcgattttagtagtggtagtgttttactatttgataaatgattagattgagaattgaacaaatggaatatacgcaatcaatgagaaatagagcctttgcttctaagggttttcctagaggggcattatatttgtttgatgaattagataattttttaactaaaaaaatgatctgcacccaaaagttggacactacacccaactagttaataagatgcagattttt Mask Tandem Repeat Region ================================================ aaaaatctgcatcttattaactagttgggtgtagtgtccaacttttgggtgcagatcatttttttagttaaaaaattatctaattcatcaaacaaatataatgcccctctaggaaaacccttagaagcaaaggctctatttctcattgattgcgtatattccatttgttcaattctcaatctaatcatttatcaaatagtaaaacactaccactactaaaatcgaccgcacttta Find is-nt database================================================ Query_seq: AA01549:AA01550|AA01549:AA01550:hypothetical protein:hypothetical protein:->->:1037941..1038175 235 aaaaatctgcatcttattaactagttgggtgtagtgtccaacttttgggtgcagatcatttttttagttaaaaaattatctaattcatcaaacaaatataatgcccctctaggaaaacccttagaagcaaaggctctatttctcattgattgcgtatattccatttgttcaattctcaatctaatcatttatcaaatagtaaaacactaccactactaaaatcgaccgcacttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01549:AA01550|AA01549:AA01550:hypothetical protein:hypothetical protein:->->:1037941..1038175 235 aaaaatctgcatcttattaactagttgggtgtagtgtccaacttttgggtgcagatcatttttttagttaaaaaattatctaattcatcaaacaaatataatgcccctctaggaaaacccttagaagcaaaggctctatttctcattgattgcgtatattccatttgttcaattctcaatctaatcatttatcaaatagtaaaacactaccactactaaaatcgaccgcacttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01549:AA01550|AA01549:AA01550:hypothetical protein:hypothetical protein:->->:1037941..1038175 235 aaaaatctgcatcttattaactagttgggtgtagtgtccaacttttgggtgcagatcatttttttagttaaaaaattatctaattcatcaaacaaatataatgcccctctaggaaaacccttagaagcaaaggctctatttctcattgattgcgtatattccatttgttcaattctcaatctaatcatttatcaaatagtaaaacactaccactactaaaatcgaccgcacttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaaatctgcatcttattaactagttgggtgtagtgtccaacttttgggtgcagatcatttttttagttaaaaaattatctaattcatcaaacaaatataatgcccctctaggaaaacccttagaagcaaaggctctatttctcattgattgcgtatattccatttgttcaattctcaatctaatcatttatcaaatagtaaaacactaccactactaaaatcgaccgcacttta Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 25 End: 216 ........................ M G V V S N F W V Q I I F L V K K L S N S S N K Y N A P L G K P L E A K A L F L I D C V Y S I C S I L N L I I Y Q I V K ................... Protein_Len: 55 Strand: - Start: 24 End: 188 ....................... N P T T D L K Q T C I M K K T L F N D L E D F L Y L A G R P F G K S A F A R N R M S Q T Y E M Q E I R L R I M ............................................... aaaaatctgcatcttattaactagttgggtgtagtgtccaacttttgggtgcagatcatttttttagttaaaaaattatctaattcatcaaacaaatataatgcccctctaggaaaacccttagaagcaaaggctctatttctcattgattgcgtatattccatttgttcaattctcaatctaatcatttatcaaatagtaaaacactaccactactaaaatcgaccgcacttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaaatctgcatcttattaactagttgggtgtagtgtccaacttttgggtgcagatcatttttttagttaaaaaattatctaattcatcaaacaaatataatgcccctctaggaaaacccttagaagcaaaggctctatttctcattgattgcgtatattccatttgttcaattctcaatctaatcatttatcaaatagtaaaacactaccactactaaaatcgaccgcacttta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 45 HP_score: -3.9 Tail_Score: -2.77899 Start: 54 End: 80 Full_Region: CAACTTTTGGGTGCA GATCATTTTTT TAGTT AAAAAATTATC TAATTCATCAAACAA .....................................................GATCATTTTTTTAGTTAAAAAATTATC........................................................................................................................................................... Find igs database================================================ Query_seq: AA01549:AA01550|AA01549:AA01550:hypothetical protein:hypothetical protein:->->:1037941..1038175 235 aaaaatctgcatcttattaactagttgggtgtagtgtccaacttttgggtgcagatcatttttttagttaaaaaattatctaattcatcaaacaaatataatgcccctctaggaaaacccttagaagcaaaggctctatttctcattgattgcgtatattccatttgttcaattctcaatctaatcatttatcaaatagtaaaacactaccactactaaaatcgaccgcacttta Intra-Species Hit: Count: 1 Min: 1 Max: 235 Len: 235 Subject: aact_AA01549_AA01550|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1037941,1038175]|235 HSP 1 e-value: 1.0E-118 bit: 424.0 Len: 235 Query Start:1 Query End:235 Subject Strand: POSITIVE Subject Start: 1 Subject End: 235 aaaaatctgcatcttattaactagttgggtgtagtgtccaacttttgggtgcagatcannnnnnnagttaaaaaattatctaattcatcaaacaaatataatgcccctctaggaaaacccttagaagcaaaggctctatttctcattgattgcgtatattccatttgttcaattctcaatctaatcatttatcaaatagtaaaacactaccactactaaaatcgaccgcacttta aaaaatctgcatcttattaactagttgggtgtagtgtccaacttttgggtgcagatcatttttttagttaaaaaattatctaattcatcaaacaaatataatgcccctctaggaaaacccttagaagcaaaggctctatttctcattgattgcgtatattccatttgttcaattctcaatctaatcatttatcaaatagtaaaacactaccactactaaaatcgaccgcacttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAAUCUGCAUCUUAUUAACUAGUUGGGUGUAGUGUCCAACUUUUGGGUGCAGAUCAUUUUUUUAGUUAAAAAAUUAUCUAAUUCAUCAAACAAAUAUAAUGCCCCUCUAGGAAAACCCUUAGAAGCAAAGGCUCUAUUUCUCAUUGAUUGCGUAUAUUCCAUUUGUUCAAUUCUCAAUCUAAUCAUUUAUCAAAUAGUAAAACACUACCACUACUAAAAUCGACCGCACUUUA ....((((((((((........(((((((.......)))))))...)))))))))).....(((((((......................(((((((....(((...((((((......)))))).)))...((...(((.......))).)).........)))))))...........................((((.....)))).....))))))).............. (-35.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAGUGCGGUCGAUUUUAGUAGUGGUAGUGUUUUACUAUUUGAUAAAUGAUUAGAUUGAGAAUUGAACAAAUGGAAUAUACGCAAUCAAUGAGAAAUAGAGCCUUUGCUUCUAAGGGUUUUCCUAGAGGGGCAUUAUAUUUGUUUGAUGAAUUAGAUAAUUUUUUAACUAAAAAAAUGAUCUGCACCCAAAAGUUGGACACUACACCCAACUAGUUAAUAAGAUGCAGAUUUUU .....((((.((....((((((((.((((((((..(((.((((...................((..((((((((........((..((.((.....)).))))...(((((((..(((....)))..)))))))...))))))))..))....(((((.(((((((.....))))))).)))))....)))))))..)))))))).....)))).))))...))))))....... (-50.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 533 58 22 72 35 aact:AA02681|5end_hypothetical_protein_1865749..1865979_POSITIVE 517 58 24 48 13 aact:AA01176|5end_hypothetical_protein_806309..806539_POSITIVE 442 58 13 130 78 aact:AA00145|5end_probable_degenerate_transposase_fragment_105046..105276_POSITIVE 434 59 24 37 1 aact:AA02629|5end_hypothetical_protein_1830072..1830302_POSITIVE 426 79 35 145 101 aact:AA01444|5end_ISRS011/IS150_related_transposase,_orfB_969159..969389_POSITIVE 407 66 22 144 105 aact:AA00535|5end_hypothetical_protein_365695..365925_POSITIVE 396 69 23 132 91 aact:AA00008|5end_hypothetical_protein_(possible_transposase_fragment)_5032..5262_POSITIVE 380 63 35 130 101 aact:AA01287|5end_transposase_871583..871813_POSITIVE 347 64 35 177 145 aact:AA00226|5end_hypothetical_protein_156276..156506_POSITIVE 345 63 23 127 91 aact:AA02115|5end_hypothetical_protein_1444528..1444758_POSITIVE 339 70 26 141 95 aact:AA01816|5end_hypothetical_protein_1223956..1224186_POSITIVE 339 58 19 173 127 aact:AA00633|5end_hypothetical_protein_434971..435201_POSITIVE 332 49 4 112 78 aact:AA02340|5end_hypothetical_protein_1598887..1599117_POSITIVE 332 49 4 126 92 aact:AA01267|5end_degenerate_transposase_fragment_862267..862497_POSITIVE 332 49 4 112 78 aact:AA01075|5end_hypothetical_protein_745605..745835_POSITIVE 332 63 22 139 92 aact:AA00643|5end_hypothetical_protein_440432..440662_POSITIVE 331 58 22 65 25 aact:AA02419|5end_hypothetical_protein_1661731..1661961_POSITIVE 283 154 111 144 93 aact:AA02252|5end_conserved_hypothetical_protein_1533840..1534070_POSITIVE 279 68 24 179 140 aact:AA00978|5end_conserved_hypothetical_protein_683399..683629_POSITIVE 272 57 12 202 153 aact:AA00537|5end_conserved_hypothetical_protein_367992..368222_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 533 58 22 54 17 aact:AA02679|3end_formate_dehydrogenase,_gamma_subunit_1865811..1865961_POSITIVE 517 58 24 54 19 aact:AA01175|3end_6-phosphogluconate_dehydrogenase_806395..806545_POSITIVE 457 50 1 149 103 aact:AA02629|3end_hypothetical_protein_1830274..1830424_POSITIVE 426 79 35 99 55 aact:AA01442|3end_phosphatidylserine_synthetase_969193..969343_POSITIVE 398 66 23 85 46 aact:AA01402|3end_beta_1-4_glucosyltransferase_948382..948532_POSITIVE 396 69 23 75 34 aact:AA00007|3end_superoxide_dismutase_[Cu-Zn]_precursor_5055..5205_POSITIVE 345 63 23 89 53 aact:AA02114|3end_exodeoxyribonuclease_I_1444570..1444720_POSITIVE 339 70 26 90 44 aact:AA01815|3end_probable_sodium-dependent_transmembrane_transport_protein_1223985..1224135_POSITIVE 332 49 4 50 16 aact:AA01074|3end_diacylglycerol_kinase_745623..745773_POSITIVE 332 63 22 89 42 aact:AA00642|3end_conserved_hypothetical_protein_440462..440612_POSITIVE 331 58 22 65 25 aact:AA02418|3end_peptidyl-tRNA_hydrolase_1661811..1661961_POSITIVE 285 58 11 40 3 aact:AA02338|3end_conserved_hypothetical_protein;_possible_integral_membrane_protein_1598885..1599035_POSITIVE 285 58 11 40 3 aact:AA01266|3end_hypothetical_protein_862251..862401_POSITIVE 283 154 111 67 16 aact:AA02251|3end_biotin_sulfoxide_reductase;_Trimethylamine-N-oxide_reductase_precursor_1533843..1533993_POSITIVE 272 70 26 79 26 aact:AA00075|3end_conserved_hypothetical_protein_55636..55786_POSITIVE 263 58 22 50 10 aact:AA00221|3end_NADP-specific_glutamate_dehydrogenase_153865..154015_POSITIVE 260 58 29 51 14 aact:AA02936|3end_lipoprotein_E_precursor;_outer_membrane_protein_P4_precursor;_acid_phosphatase_2056373..2056523_POSITIVE 259 59 27 40 9 aact:AA01751|3end_conserved_hypothetical_protein_1185392..1185542_POSITIVE 245 156 112 140 96 aact:AA01855|3end_transcriptional_regulator,_Cro/CI_family_1247385..1247535_POSITIVE 242 183 141 49 6 aact:AA02662|3end_hypothetical_protein_1853596..1853746_POSITIVE