Origin IGS: tggtaactccttactgcaacaattaattaagggaattatagcgaaaataaattttaggaacgtgatctgtataacatttttgaggttttttattgggtagggggatttttgtgagatttgtaacagtgaggagaaagaaaagtgatctaccctaccaaaaaaaactggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaattatcaagccggtcaccgagcaggatttcggtatatgagcggtggctaaatttcatcagatttctcgttctcaagtcatttattggaaaagcctttcgtgaaagagggcttgatggcgtaaaatcgccttatacaaacctaaaacctccaaaattctgaaaccccaaatgaaaagaacgcagattgaagtggctgaaacaagtgacttttcaccacaagcctttaaaaaactgaaacgggaaaccgataagcttcaatacggtgttgaatgccgacctttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaaggtcggcattcaacaccgtattgaagcttatcggtttcccgtttcagttttttaaaggcttgtggtgaaaagtcacttgtttcagccacttcaatctgcgttcttttcatttggggtttcagaattttggaggttttaggtttgtataaggcgattttacgccatcaagccctctttcacgaaaggcttttccaataaatgacttgagaacgagaaatctgatgaaatttagccaccgctcatataccgaaatcctgctcggtgaccggcttgataatttttaaacgaaattggtaagagtaagacataatctgcacctcaaattaggtgtccagttttttttggtagggtagatcacttttctttctcctcactgttacaaatctcacaaaaatccccctacccaataaaaaacctcaaaaatgttatacagatcacgttcctaaaatttattttcgctataattcccttaattaattgttgcagtaaggagttacca Mask Tandem Repeat Region ================================================ tggtaactccttactgcaacaattaattaagggaattatagcgaaaataaattttaggaacgtgatctgtataacatttttgaggttttttattgggtagggggatttttgtgagatttgtaacagtgaggagaaagaaaagtgatctaccctaccaaaaaaaactggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaattatcaagccggtcaccgagcaggatttcggtatatgagcggtggctaaatttcatcagatttctcgttctcaagtcatttattggaaaagcctttcgtgaaagagggcttgatggcgtaaaatcgccttatacaaacctaaaacctccaaaattctgaaaccccaaatgaaaagaacgcagattgaagtggctgaaacaagtgacttttcaccacaagcctttaaaaaactgaaacgggaaaccgataagcttcaatacggtgttgaatgccgacctttta Find is-nt database================================================ Query_seq: AA01547:AA01549|AA01547:AA01549:deoxyribose-phosphate aldolase:hypothetical protein:->->:1037080..1037583 504 tggtaactccttactgcaacaattaattaagggaattatagcgaaaataaattttaggaacgtgatctgtataacatttttgaggttttttattgggtagggggatttttgtgagatttgtaacagtgaggagaaagaaaagtgatctaccctaccaaaaaaaactggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaattatcaagccggtcaccgagcaggatttcggtatatgagcggtggctaaatttcatcagatttctcgttctcaagtcatttattggaaaagcctttcgtgaaagagggcttgatggcgtaaaatcgccttatacaaacctaaaacctccaaaattctgaaaccccaaatgaaaagaacgcagattgaagtggctgaaacaagtgacttttcaccacaagcctttaaaaaactgaaacgggaaaccgataagcttcaatacggtgttgaatgccgacctttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01547:AA01549|AA01547:AA01549:deoxyribose-phosphate aldolase:hypothetical protein:->->:1037080..1037583 504 tggtaactccttactgcaacaattaattaagggaattatagcgaaaataaattttaggaacgtgatctgtataacatttttgaggttttttattgggtagggggatttttgtgagatttgtaacagtgaggagaaagaaaagtgatctaccctaccaaaaaaaactggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaattatcaagccggtcaccgagcaggatttcggtatatgagcggtggctaaatttcatcagatttctcgttctcaagtcatttattggaaaagcctttcgtgaaagagggcttgatggcgtaaaatcgccttatacaaacctaaaacctccaaaattctgaaaccccaaatgaaaagaacgcagattgaagtggctgaaacaagtgacttttcaccacaagcctttaaaaaactgaaacgggaaaccgataagcttcaatacggtgttgaatgccgacctttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01547:AA01549|AA01547:AA01549:deoxyribose-phosphate aldolase:hypothetical protein:->->:1037080..1037583 504 tggtaactccttactgcaacaattaattaagggaattatagcgaaaataaattttaggaacgtgatctgtataacatttttgaggttttttattgggtagggggatttttgtgagatttgtaacagtgaggagaaagaaaagtgatctaccctaccaaaaaaaactggacacctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaattatcaagccggtcaccgagcaggatttcggtatatgagcggtggctaaatttcatcagatttctcgttctcaagtcatttattggaaaagcctttcgtgaaagagggcttgatggcgtaaaatcgccttatacaaacctaaaacctccaaaattctgaaaccccaaatgaaaagaacgcagattgaagtggctgaaacaagtgacttttcaccacaagcctttaaaaaactgaaacgggaaaccgataagcttcaatacggtgttgaatgccgacctttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 132 Max: 278 Len: 147 Subject: UniRef90_Q7WRX1 Cluster: Hypothetical protein orf3; n=1; Haemophilus influenzae|Rep: Hypothetical protein orf3 - Haemophilus influenzae HSP 1 e-value: 7.0E-8 bit: 58.9 Len: 147 Query Start:132 Query End:278 Subject Strand: null Subject Start: 1 Subject End: 47 ................................................................................................................................... M K F S H R S Y T E I L L G D R L D N F * T K L V R V R H N L H L K L G V Q F F L V G * I T F L S .................................................................................................................................................................................................................................. ................................................................................................................................... M K F S H L T D T I I L F G D E F D N F Q T K L I R V G H N L H L K L G V Q - - I L G C S S F I S .................................................................................................................................................................................................................................. tggtaactccttactgcaacaattaattaagggaattatagcgaaaataaattttaggaacgtgatctgtataacatttttgaggttttttattgggtagggggatttttgtgagatttgtaacagtgaggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncagatttctcgttctcaagtcatttattggaaaagcctttcgtgaaagagggcttgatggcgtaaaatcgccttatacaaacctaaaacctccaaaattctgaaaccccaaatgaaaagaacgcagattgaagtggctgaaacaagtgacttttcaccacaagcctttaaaaaactgaaacgggaaaccgataagcttcaatacggtgttgaatgccgacctttta Predict ORF larger than 30AA ================================================ Protein_Len: 44 Strand: + Start: 247 End: 378 ...................................................................................................................................................................................................................................................... M S V Y E R W L N F I R F L V L K S F I G K A F R E R G L D G V K S P Y T N L K P P K F .............................................................................................................................. Protein_Len: 53 Strand: + Start: 345 End: 503 ........................................................................................................................................................................................................................................................................................................................................................ M A L Y K P K T S K I L K P Q M K R T Q I E V A E T S D F S P Q A F K K L K R E T D K L Q Y G V E C R P F . Protein_Len: 34 Strand: - Start: 305 End: 406 ................................................................................................................................................................................................................................................................................................................ Q F L R E H F L A Q H R L I A K Y L G L V E L I R F G W I F L V C M .................................................................................................. Protein_Len: 59 Strand: - Start: 1 End: 177 P L E K S C C N I L P I I A F I F K L F T I Q I V N K L N K I P Y P S K Q S I Q L L S S F S F H D V R G F F V P C R M ....................................................................................................................................................................................................................................................................................................................................... tggtaactccttactgcaacaattaattaagggaattatagcgaaaataaattttaggaacgtgatctgtataacatttttgaggttttttattgggtagggggatttttgtgagatttgtaacagtgaggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncagatttctcgttctcaagtcatttattggaaaagcctttcgtgaaagagggcttgatggcgtaaaatcgccttatacaaacctaaaacctccaaaattctgaaaccccaaatgaaaagaacgcagattgaagtggctgaaacaagtgacttttcaccacaagcctttaaaaaactgaaacgggaaaccgataagcttcaatacggtgttgaatgccgacctttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tggtaactccttactgcaacaattaattaagggaattatagcgaaaataaattttaggaacgtgatctgtataacatttttgaggttttttattgggtagggggatttttgtgagatttgtaacagtgaggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncagatttctcgttctcaagtcatttattggaaaagcctttcgtgaaagagggcttgatggcgtaaaatcgccttatacaaacctaaaacctccaaaattctgaaaccccaaatgaaaagaacgcagattgaagtggctgaaacaagtgacttttcaccacaagcctttaaaaaactgaaacgggaaaccgataagcttcaatacggtgttgaatgccgacctttta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 44 HP_score: -2.5 Tail_Score: -3.18768 Start: 174 End: 192 Full_Region: AAAAAACTGGACACC TAATTTG AGGTG CAGATTA TGTCTTACTCTTACC .............................................................................................................................................................................TAATTTGAGGTGCAGATTA........................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 42 HP_score: -2.5 Tail_Score: -2.87931 Start: 238 End: 253 Full_Region: TTATCAAGCCGGTCA CCGAG CAGGAT TTCGG TATATGAGCGGTGGC .............................................................................................................................................................................................................................................CCGAGCAGGATTTCGG........................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: AA01547:AA01549|AA01547:AA01549:deoxyribose-phosphate aldolase:hypothetical protein:->->:1037080..1037583 504 tggtaactccttactgcaacaattaattaagggaattatagcgaaaataaattttaggaacgtgatctgtataacatttttgaggttttttattgggtagggggatttttgtgagatttgtaacagtgaggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncagatttctcgttctcaagtcatttattggaaaagcctttcgtgaaagagggcttgatggcgtaaaatcgccttatacaaacctaaaacctccaaaattctgaaaccccaaatgaaaagaacgcagattgaagtggctgaaacaagtgacttttcaccacaagcctttaaaaaactgaaacgggaaaccgataagcttcaatacggtgttgaatgccgacctttta Intra-Species Hit: Count: 3 Min: 1 Max: 504 Len: 504 Subject: aact_AA01547_AA01549|deoxyribose-phosphate aldolase:hypothetical protein|POSITIVE:POSITIVE|[1037080,1037583]|504 HSP 1 e-value: 1.0E-125 bit: 448.0 Len: 226 Query Start:279 Query End:504 Subject Strand: POSITIVE Subject Start: 279 Subject End: 504 ......................................................................................................................................................................................................................................................................................cagatttctcgttctcaagtcatttattggaaaagcctttcgtgaaagagggcttgatggcgtaaaatcgccttatacaaacctaaaacctccaaaattctgaaaccccaaatgaaaagaacgcagattgaagtggctgaaacaagtgacttttcaccacaagcctttaaaaaactgaaacgggaaaccgataagcttcaatacggtgttgaatgccgacctttta ......................................................................................................................................................................................................................................................................................cagatttctcgttctcaagtcatttattggaaaagcctttcgtgaaagagggcttgatggcgtaaaatcgccttatacaaacctaaaacctccaaaattctgaaaccccaaatgaaaagaacgcagattgaagtggctgaaacaagtgacttttcaccacaagcctttaaaaaactgaaacgggaaaccgataagcttcaatacggtgttgaatgccgacctttta HSP 2 e-value: 7.0E-67 bit: 256.0 Len: 129 Query Start:1 Query End:129 Subject Strand: POSITIVE Subject Start: 1 Subject End: 129 tggtaactccttactgcaacaattaattaagggaattatagcgaaaataaattttaggaacgtgatctgtataacatttttgaggttttttattgggtagggggatttttgtgagatttgtaacagtga....................................................................................................................................................................................................................................................................................................................................................................................... tggtaactccttactgcaacaattaattaagggaattatagcgaaaataaattttaggaacgtgatctgtataacatttttgaggttttttattgggtagggggatttttgtgagatttgtaacagtga....................................................................................................................................................................................................................................................................................................................................................................................... Subject: aact_AA00227_AA00229|hypothetical sequence:DNA polymerase III, alpha subunit|POSITIVE:POSITIVE|[156703,157002]|300 HSP 1 e-value: 5.0E-52 bit: 206.0 Len: 124 Query Start:282 Query End:405 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 124 .........................................................................................................................................................................................................................................................................................atttctcgttctcaagtcatttattggaaaagcctttcgtgaaagagggcttgatggcgtaaaatcgccttatacaaacctaaaacctccaaaattctgaaaccccaaatgaaaagaacgcaga................................................................................................... .........................................................................................................................................................................................................................................................................................atttctcgttctcaagtcatttattggaaaagcctttcgtgaaagagggcttgatggcgtaaaatcaccttacacaaacctaaaacctccaaaattatgaaacccaaaatgaaaaaaacgcaga................................................................................................... Subject: aact_AA01402_AA01404|beta 1-4 glucosyltransferase:hypothetical protein|POSITIVE:POSITIVE|[948443,948717]|275 HSP 1 e-value: 1.0E-37 bit: 159.0 Len: 127 Query Start:279 Query End:405 Subject Strand: POSITIVE Subject Start: 146 Subject End: 275 ......................................................................................................................................................................................................................................................................................cagatttctcgttctcaagtcatttattgga---aaagcctttcgtgaaagagggcttgatggcgtaaaatcgccttatacaaacctaaaacctccaaaattctgaaaccccaaatgaaaagaacgcaga................................................................................................... ......................................................................................................................................................................................................................................................................................cagattcctcattctcaagtcatttattgtggttaaagcctttcgtgaaagaaggcttgatggcgtaaaatcaccttacacaaacctaaaacctccaaaattctgaaacccaaaatgaaaaaaacgcaga................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGGUAACUCCUUACUGCAACAAUUAAUUAAGGGAAUUAUAGCGAAAAUAAAUUUUAGGAACGUGAUCUGUAUAACAUUUUUGAGGUUUUUUAUUGGGUAGGGGGAUUUUUGUGAGAUUUGUAACAGUGAGGAGAAAGAAAAGUGAUCUACCCUACCAAAAAAAACUGGACACCUAAUUUGAGGUGCAGAUUAUGUCUUACUCUUACCAAUUUCGUUUAAAAAUUAUCAAGCCGGUCACCGAGCAGGAUUUCGGUAUAUGAGCGGUGGCUAAAUUUCAUCAGAUUUCUCGUUCUCAAGUCAUUUAUUGGAAAAGCCUUUCGUGAAAGAGGGCUUGAUGGCGUAAAAUCGCCUUAUACAAACCUAAAACCUCCAAAAUUCUGAAACCCCAAAUGAAAAGAACGCAGAUUGAAGUGGCUGAAACAAGUGACUUUUCACCACAAGCCUUUAAAAAACUGAAACGGGAAACCGAUAAGCUUCAAUACGGUGUUGAAUGCCGACCUUUUA .((((.........................(((..((((.......)))).((((((((..((..........))..(((.((((((((...(((..(((((.(((((((((((((((.((((.(((((((..........(((((((......(((........))).(((((......))))).))))))).))))))).)))).))))))..........(((((((((.(((((((..((..((....))...))..)))))))....((((((..((.......(((.(((........)))))).......))))))))...)))))))))))).)))))).)))))..)))....)))))))).))).)))))))).)))..........(((((..((((((((((((.......((((....))))....))))...............(((....)))....))))))))...)))))...))))......... (-125.94) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAGGUCGGCAUUCAACACCGUAUUGAAGCUUAUCGGUUUCCCGUUUCAGUUUUUUAAAGGCUUGUGGUGAAAAGUCACUUGUUUCAGCCACUUCAAUCUGCGUUCUUUUCAUUUGGGGUUUCAGAAUUUUGGAGGUUUUAGGUUUGUAUAAGGCGAUUUUACGCCAUCAAGCCCUCUUUCACGAAAGGCUUUUCCAAUAAAUGACUUGAGAACGAGAAAUCUGAUGAAAUUUAGCCACCGCUCAUAUACCGAAAUCCUGCUCGGUGACCGGCUUGAUAAUUUUUAAACGAAAUUGGUAAGAGUAAGACAUAAUCUGCACCUCAAAUUAGGUGUCCAGUUUUUUUUGGUAGGGUAGAUCACUUUUCUUUCUCCUCACUGUUACAAAUCUCACAAAAAUCCCCCUACCCAAUAAAAAACCUCAAAAAUGUUAUACAGAUCACGUUCCUAAAAUUUAUUUUCGCUAUAAUUCCCUUAAUUAAUUGUUGCAGUAAGGAGUUACCA .....(((.(((.(((((.......)))))..(((((((((((.((((((((((.((((..(((((((((((.((((((.(((((...((((.((((((((((.(..((........))..)..))))...))))))......))))...)))))..)))))).))))).))))))........(....)..........)))).))).)))).))).)))).)))))))......)))))).......((((((........))))))...(((..(((((.(((((...(((..((((....(((.(((((.....((((((......))))))...(((((((..(((((((.(((.......)))..........(((..........))).......)))))))....))))))).......))))).)))..))))..))).))))).)))))...))).(((((((((((((.....)))).))...)))))))... (-116.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 893 340 291 111 62 aact:AA01445|5end_hypothetical_protein_969899..970129_POSITIVE 764 208 161 231 183 aact:AA01446|5end_conserved_hypothetical_protein_970151..970381_POSITIVE 717 210 161 184 134 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 606 230 181 226 176 aact:AA00229|5end_DNA_polymerase_III,_alpha_subunit_156863..157093_POSITIVE 453 499 457 115 67 aact:AA02420|5end_ISRSO11_related_transposase_1662070..1662300_POSITIVE 432 269 225 196 135 aact:AA00383|5end_chloride_channel_protein_258894..259124_POSITIVE 400 267 228 176 131 aact:AA02407|5end_thymidine_kinase_1652666..1652896_POSITIVE 391 268 230 39 4 aact:AA01286|5end_5,10-methylenetetrahydrofolate_dehydrogenase_(bifunctional_protein)_870309..870539_POSITIVE 388 268 223 209 149 aact:AA00507|5end_hypothetical_protein_352996..353226_POSITIVE 362 265 225 143 95 aact:AA01654|5end_periplasmic_maltose-binding_protein_precursor_1119896..1120126_POSITIVE 360 269 221 108 46 aact:AA02410|5end_cell_division_protein_1655429..1655659_POSITIVE 359 268 229 176 134 aact:AA02705|5end_hypothetical_protein_1886717..1886947_POSITIVE 357 270 224 190 148 aact:AA01858|5end_conserved_hypothetical_protein_1250699..1250929_POSITIVE 357 190 143 162 111 aact:AA00674|5end_nicotinate-nucleotide-dimethylbenzimidazole_phosphoribosyltransferase_461810..462040_POSITIVE 353 268 230 70 26 aact:AA02205|5end_short_chain_dehydrogenase/reductase_1501170..1501400_POSITIVE 353 268 225 111 63 aact:AA01128|5end_hypothetical_protein_774030..774260_POSITIVE 350 269 230 84 42 aact:AA02157|5end_TonB-dependent_outer_membrane_receptor_1475251..1475481_POSITIVE 346 268 231 196 145 aact:AA01051|5end_iron_(III)_ABC_transporter,_ATP-binding_protein_732043..732273_POSITIVE 341 310 261 167 123 aact:AA02826|5end_conserved_hypothetical_protein_1986336..1986566_POSITIVE 339 331 298 140 107 aact:AA02883|5end_conserved_hypothetical_protein_2021418..2021648_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 864 370 321 55 6 aact:AA00227|3end_hypothetical_sequence_156642..156792_POSITIVE 786 210 161 109 59 aact:AA01445|3end_hypothetical_protein_970107..970257_POSITIVE 717 210 161 123 73 aact:AA01289|3end_hypothetical_protein_872848..872998_POSITIVE 432 269 225 107 46 aact:AA00382|3end_hypothetical_protein_258885..259035_POSITIVE 354 340 293 96 47 aact:AA02674|3end_formate_dehydrogenase-related_protein_1860732..1860882_POSITIVE 353 268 230 82 38 aact:AA02204|3end_hypothetical_protein_1501262..1501412_POSITIVE 350 269 230 79 37 aact:AA02156|3end_conserved_hypothetical_protein_1475326..1475476_POSITIVE 341 310 261 150 106 aact:AA02824|3end_conserved_hypothetical_protein_1986399..1986549_POSITIVE 339 331 298 65 32 aact:AA02881|3end_50S_ribosomal_protein_L27_2021423..2021573_POSITIVE 325 499 453 57 4 aact:AA01138|3end_anaerobic_dimethyl_sulfoxide_reductase,_chain_B_781685..781835_POSITIVE 324 270 228 148 121 aact:AA02404|3end_hypothetical_protein_1652736..1652886_POSITIVE 320 140 93 61 11 aact:AA02442|3end_thioredoxin-family_protein_1686272..1686422_POSITIVE 315 268 227 150 112 aact:AA01379|3end_glycerophosphoryl_diester_phosphodiesterase_(protein_D)_934188..934338_POSITIVE 313 269 228 151 125 aact:AA02405|3end_conserved_hypothetical_protein_1652740..1652890_POSITIVE 313 267 221 91 41 aact:AA02225|3end_ABC_transporter_ATP-binding_protein_1514592..1514742_POSITIVE 311 189 146 59 4 aact:AA01778|3end_aminopeptidase_A/I;_cytosol_aminopeptidase;_leucyl_aminopeptidase_1199965..1200115_POSITIVE 309 280 232 107 34 aact:AA02409|3end_DNA_ligase_1655404..1655554_POSITIVE 308 268 228 49 1 aact:AA02677|3end_formate_dehydrogenase,_beta_subunit_1865105..1865255_POSITIVE 306 268 230 48 7 aact:AA00241|3end_formate_acetyltransferase_activating_enzyme_(anaerobic_ribonucleoside-triphosphate_reductase_activating_protein)_172090..172240_POSITIVE 305 388 346 47 11 aact:AA01857|3end_glutamate-ammonia-ligase_adenylyltransferase_1250357..1250507_POSITIVE