Origin IGS: tagtaatttttttgttgcatacacttcgtttctccaaataatttcaagtccaattcccgtacttgccctcggcttttaaagcctataaagtgcggtcgttttttccggcgtttttca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaaaaacgccggaaaaaacgaccgcactttataggctttaaaagccgagggcaagtacgggaattggacttgaaattatttggagaaacgaagtgtatgcaacaaaaaaattacta Mask Tandem Repeat Region ================================================ tagtaatttttttgttgcatacacttcgtttctccaaataatttcaagtccaattcccgtacttgccctcggcttttaaagcctataaagtgcggtcgttttttccggcgtttttca Find is-nt database================================================ Query_seq: AA01534:AA01537|AA01534:AA01537:conserved hypothetical protein (possible GTP pyrophosphokinase):possible glucokinase regulatory protein:->->:1030639..1030755 117 tagtaatttttttgttgcatacacttcgtttctccaaataatttcaagtccaattcccgtacttgccctcggcttttaaagcctataaagtgcggtcgttttttccggcgtttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01534:AA01537|AA01534:AA01537:conserved hypothetical protein (possible GTP pyrophosphokinase):possible glucokinase regulatory protein:->->:1030639..1030755 117 tagtaatttttttgttgcatacacttcgtttctccaaataatttcaagtccaattcccgtacttgccctcggcttttaaagcctataaagtgcggtcgttttttccggcgtttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01534:AA01537|AA01534:AA01537:conserved hypothetical protein (possible GTP pyrophosphokinase):possible glucokinase regulatory protein:->->:1030639..1030755 117 tagtaatttttttgttgcatacacttcgtttctccaaataatttcaagtccaattcccgtacttgccctcggcttttaaagcctataaagtgcggtcgttttttccggcgtttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagtaatttttttgttgcatacacttcgtttctccaaataatttcaagtccaattcccgtacttgccctcggcttttaaagcctataaagtgcggtcgttttttccggcgtttttca Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 19 End: 117 .................. M H F V S P N N F K S N S R T C P R L L K P I K C G R F F R R F S tagtaatttttttgttgcatacacttcgtttctccaaataatttcaagtccaattcccgtacttgccctcggcttttaaagcctataaagtgcggtcgttttttccggcgtttttca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagtaatttttttgttgcatacacttcgtttctccaaataatttcaagtccaattcccgtacttgccctcggcttttaaagcctataaagtgcggtcgttttttccggcgtttttca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01534:AA01537|AA01534:AA01537:conserved hypothetical protein (possible GTP pyrophosphokinase):possible glucokinase regulatory protein:->->:1030639..1030755 117 tagtaatttttttgttgcatacacttcgtttctccaaataatttcaagtccaattcccgtacttgccctcggcttttaaagcctataaagtgcggtcgttttttccggcgtttttca Intra-Species Hit: Count: 1 Min: 14 Max: 117 Len: 104 Subject: aact_AA01534_AA01537|conserved hypothetical protein (possible GTP pyrophosphokinase):possible glucokinase regulatory protein|POSITIVE:POSITIVE|[1030639,1030755]|117 HSP 1 e-value: 1.0E-52 bit: 206.0 Len: 104 Query Start:14 Query End:117 Subject Strand: POSITIVE Subject Start: 14 Subject End: 117 .............gttgcatacacttcgtttctccaaataatttcaagtccaattcccgtacttgccctcggcttttaaagcctataaagtgcggtcgttttttccggcgtttttca .............gttgcatacacttcgtttctccaaataatttcaagtccaattcccgtacttgccctcggcttttaaagcctataaagtgcggtcgttttttccggcgtttttca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUGCAUACACUUCGUUUCUCCAAAUAAUUUCAAGUCCAAUUCCCGUACUUGCCCUCGGCUUUUAAAGCCUAUAAAGUGCGGUCGUUUUUUCCGGCGUUUUUCA ..................................(((.(((..((((((((......((((.....))))....))))))))..)))......)))........ (-16.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAAAACGCCGGAAAAAACGACCGCACUUUAUAGGCUUUAAAAGCCGAGGGCAAGUACGGGAAUUGGACUUGAAAUUAUUUGGAGAAACGAAGUGUAUGCAAC ........((((.......))...(((((((...((((.....)))).....(((((.((.....)).))))).................)))))))..))... (-17.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 468 100 56 120 72 aact:AA00198|5end_conserved_hypothetical_protein_(Cytosine_deaminase-related_enzyme)_138039..138269_POSITIVE 446 100 57 62 25 aact:AA00582|5end_conserved_hypothetical_protein_397904..398134_POSITIVE 436 100 51 58 8 aact:AA00602|5end_1-deoxy-D-xylulose_5-phosphate_reductoisomerase_409077..409307_POSITIVE 425 100 55 59 16 aact:AA01890|5end_cell-cycle_protein_1274544..1274774_POSITIVE 418 100 52 183 120 aact:AA01991|5end_conserved_hypothetical_protein_(possible_nitroreductase)_1352372..1352602_POSITIVE 413 100 51 182 127 aact:AA02915|5end_thioredoxin_m_2040652..2040882_POSITIVE 411 100 57 125 82 aact:AA01059|5end_orotate_phosphoribosyltransferase_735052..735282_POSITIVE 407 90 44 166 130 aact:AA01519|5end_conserved_hypothetical_protein_1021794..1022024_POSITIVE 406 100 51 168 118 aact:AA00511|5end_nicotinamide_mononucleotide_transporter_353884..354114_POSITIVE 398 100 56 170 131 aact:AA03003|5end_GTP-binding_membrane_protein_2096544..2096774_POSITIVE 398 100 56 49 10 aact:AA03002|5end_hypothetical_protein_2096423..2096653_POSITIVE 393 100 56 224 184 aact:AA01598|5end_conserved_hypothetical_protein_1078954..1079184_POSITIVE 390 100 52 208 162 aact:AA01284|5end_heat_shock_protein_(hsp60)_868574..868804_POSITIVE 388 89 41 122 64 aact:AA00458|5end_nickel-binding_periplasmic_protein_319374..319604_POSITIVE 386 100 58 180 139 aact:AA01173|5end_VapD-homolog_803850..804080_POSITIVE 377 89 43 164 114 aact:AA01385|5end_50S_ribosomal_protein_L9_935509..935739_POSITIVE 375 100 51 105 41 aact:AA00618|5end_molybdopterin_biosynthesis_protein_422984..423214_POSITIVE 374 95 68 100 73 aact:AA02577|5end_nuclease_1795267..1795497_POSITIVE 370 100 53 181 137 aact:AA00501|5end_hypothetical_protein_347293..347523_POSITIVE 369 84 43 116 78 aact:AA02076|5end_conserved_hypothetical_protein_1409926..1410156_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 447 100 52 44 1 aact:AA00581|3end_thiosulfate_sulfurtransferase_(rhodanese)_397966..398116_POSITIVE 407 90 44 83 47 aact:AA01517|3end_peptidyl-prolyl_cis-trans_isomerase;_macrophage_infectivity_protein_1021791..1021941_POSITIVE 406 100 51 87 37 aact:AA00508|3end_SAM-dependent_methyltransferase_353883..354033_POSITIVE 402 97 65 93 63 aact:AA02253|3end_hypothetical_protein_1534786..1534936_POSITIVE 393 100 56 126 86 aact:AA01597|3end_oligoribonuclease_1078936..1079086_POSITIVE 390 100 52 84 38 aact:AA01280|3end_groES_chaperonin_868530..868680_POSITIVE 389 100 74 33 7 aact:AA03001|3end_probable_glycyl_radical_protein/pyruvate-formate_lyase_2096487..2096637_POSITIVE 386 100 58 97 56 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 377 89 43 115 65 aact:AA01384|3end_hypothetical_protein_935540..935690_POSITIVE 370 100 53 87 43 aact:AA00499|3end_pyridoxamine-5'-phosphate_oxidase_347279..347429_POSITIVE 369 84 43 89 51 aact:AA02075|3end_conserved_hypothetical_protein_1409979..1410129_POSITIVE 366 100 68 85 53 aact:AA02160|3end_conserved_hypothetical_protein_1478929..1479079_POSITIVE 361 100 56 51 10 aact:AA00833|3end_UDP-N-acetylmuramate-alanine_ligase_575218..575368_POSITIVE 358 100 55 134 96 aact:AA00623|3end_hypothetical_protein_430632..430782_POSITIVE 355 100 74 40 14 aact:AA01057|3end_DnaJ-like_protein_djlA_735047..735197_POSITIVE 353 100 51 87 17 aact:AA00702|3end_conserved_hypothetical_protein_483848..483998_POSITIVE 349 100 66 69 37 aact:AA02758|3end_3-phosphoshikimate_1-carboxyvinyltransferase_1933150..1933300_POSITIVE 348 100 51 145 84 aact:AA02507|3end_penicillin-insensitive_murein_endopeptidase_A_1741007..1741157_POSITIVE 348 100 54 128 74 aact:AA01434|3end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_963021..963171_POSITIVE 348 89 45 53 1 aact:AA00455|3end_lipoprotein_precursor_319385..319535_POSITIVE