Origin IGS: aaaaaacgcctgtaatggaaagtgggagcatattactacgcaagggggagaagtcaatatttgtacgacagacaagcaaaagtgcggtcataaaaaaatgtattttacaccgcacctatcaagatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatcttgataggtgcggtgtaaaatacatttttttatgaccgcacttttgcttgtctgtcgtacaaatattgacttctcccccttgcgtagtaatatgctcccactttccattacaggcgtttttt Mask Tandem Repeat Region ================================================ aaaaaacgcctgtaatggaaagtgggagcatattactacgcaagggggagaagtcaatatttgtacgacagacaagcaaaagtgcggtcataaaaaaatgtattttacaccgcacctatcaagatta Find is-nt database================================================ Query_seq: AA01528:AA01530|AA01528:AA01530:probable oxidoreductase:conserved hypothetical protein:->->:1027321..1027447 127 aaaaaacgcctgtaatggaaagtgggagcatattactacgcaagggggagaagtcaatatttgtacgacagacaagcaaaagtgcggtcataaaaaaatgtattttacaccgcacctatcaagatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01528:AA01530|AA01528:AA01530:probable oxidoreductase:conserved hypothetical protein:->->:1027321..1027447 127 aaaaaacgcctgtaatggaaagtgggagcatattactacgcaagggggagaagtcaatatttgtacgacagacaagcaaaagtgcggtcataaaaaaatgtattttacaccgcacctatcaagatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01528:AA01530|AA01528:AA01530:probable oxidoreductase:conserved hypothetical protein:->->:1027321..1027447 127 aaaaaacgcctgtaatggaaagtgggagcatattactacgcaagggggagaagtcaatatttgtacgacagacaagcaaaagtgcggtcataaaaaaatgtattttacaccgcacctatcaagatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaaaacgcctgtaatggaaagtgggagcatattactacgcaagggggagaagtcaatatttgtacgacagacaagcaaaagtgcggtcataaaaaaatgtattttacaccgcacctatcaagatta Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 15 End: 125 .............. M E S G S I L L R K G E K S I F V R Q T S K S A V I K K C I L H R T Y Q D .. Protein_Len: 32 Strand: + Start: 32 End: 127 ............................... M T T Q G G E V N I C T T D K Q K C G H K K M Y F T P H L S R L Protein_Len: 38 Strand: - Start: 2 End: 115 . F V G T I S L P L M N S R L P S F D I N T R C V L L L A T M F F H I K C R M ............ aaaaaacgcctgtaatggaaagtgggagcatattactacgcaagggggagaagtcaatatttgtacgacagacaagcaaaagtgcggtcataaaaaaatgtattttacaccgcacctatcaagatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaaaacgcctgtaatggaaagtgggagcatattactacgcaagggggagaagtcaatatttgtacgacagacaagcaaaagtgcggtcataaaaaaatgtattttacaccgcacctatcaagatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01528:AA01530|AA01528:AA01530:probable oxidoreductase:conserved hypothetical protein:->->:1027321..1027447 127 aaaaaacgcctgtaatggaaagtgggagcatattactacgcaagggggagaagtcaatatttgtacgacagacaagcaaaagtgcggtcataaaaaaatgtattttacaccgcacctatcaagatta Intra-Species Hit: Count: 1 Min: 1 Max: 127 Len: 127 Subject: aact_AA01528_AA01530|probable oxidoreductase:conserved hypothetical protein|POSITIVE:POSITIVE|[1027321,1027447]|127 HSP 1 e-value: 8.0E-54 bit: 210.0 Len: 127 Query Start:1 Query End:127 Subject Strand: POSITIVE Subject Start: 1 Subject End: 127 aaaaaacgcctgtaatggaaagtgggagcatattactacgcaagggggagaagtcaatatttgtacgacagacaagcaaaagtgcggtcatnnnnnnntgtattttacaccgcacctatcaagatta aaaaaacgcctgtaatggaaagtgggagcatattactacgcaagggggagaagtcaatatttgtacgacagacaagcaaaagtgcggtcataaaaaaatgtattttacaccgcacctatcaagatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAAACGCCUGUAAUGGAAAGUGGGAGCAUAUUACUACGCAAGGGGGAGAAGUCAAUAUUUGUACGACAGACAAGCAAAAGUGCGGUCAUAAAAAAAUGUAUUUUACACCGCACCUAUCAAGAUUA ......(.(((....(((((.(((....))).)).)))....))).)....((((....(((((.(....)))))).....(((((((..(((((.......))))).))))))).......)))). (-22.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCUUGAUAGGUGCGGUGUAAAAUACAUUUUUUUAUGACCGCACUUUUGCUUGUCUGUCGUACAAAUAUUGACUUCUCCCCCUUGCGUAGUAAUAUGCUCCCACUUUCCAUUACAGGCGUUUUUU ..........(((((((((((((((.......)))))).)))))))))..((((((..((((.........))))...........(((((....)))))..............))))))....... (-30.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 444 115 74 191 145 aact:AA02902|5end_aerobic_respiration_control_protein_2031171..2031401_POSITIVE 401 107 63 127 77 aact:AA01776|5end_conserved_hypothetical_protein_1196074..1196304_POSITIVE 401 107 63 54 4 aact:AA01775|5end_hypothetical_protein_1196001..1196231_POSITIVE 382 115 76 148 102 aact:AA01648|5end_alpha-amylase_protein;_1,4-alpha-D-glucan_glucanohydrolase_1115170..1115400_POSITIVE 382 115 76 47 1 aact:AA01646|5end_hypothetical_protein_1115069..1115299_POSITIVE 350 115 78 138 100 aact:AA00126|5end_conserved_hypothetical_protein_91053..91283_POSITIVE 347 115 74 168 117 aact:AA01117|5end_signal_recognition_particle_protein_54_(GTP-binding_export_factor)_767919..768149_POSITIVE 345 115 79 174 135 aact:AA00654|5end_conserved_hypothetical_protein_447772..448002_POSITIVE 345 115 79 84 45 aact:AA00653|5end_hypothetical_protein_447682..447912_POSITIVE 331 49 5 98 39 aact:AA02888|5end_ssrA-binding_protein,_small_protein_B_2024628..2024858_POSITIVE 326 118 72 94 29 aact:AA02143|5end_hypothetical_protein_1467433..1467663_POSITIVE 323 88 43 66 15 aact:AA03008|5end_GTP-binding_protein_era_homolog_(GTPase)_2100087..2100317_POSITIVE 323 115 74 198 154 aact:AA01830|5end_2,3-cyclic-nucleotide_2-phosphodiesterase_1231556..1231786_POSITIVE 323 115 74 45 1 aact:AA01829|5end_hypothetical_protein_1231403..1231633_POSITIVE 321 115 75 165 124 aact:AA01544|5end_conserved_hypothetical_protein_1034265..1034495_POSITIVE 321 115 75 75 34 aact:AA01543|5end_hypothetical_protein_1034175..1034405_POSITIVE 320 90 42 223 182 aact:AA00169|5end_conserved_hypothetical_protein_120312..120542_POSITIVE 318 115 74 116 75 aact:AA02362|5end_conserved_hypothetical_protein_1619905..1620135_POSITIVE 313 120 76 138 88 aact:AA00091|5end_short-patch_reverse_transcription_protein_67741..67971_POSITIVE 312 115 75 217 172 aact:AA02778|5end_hypothetical_protein_1942585..1942815_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 444 115 74 78 32 aact:AA02899|3end_oligopeptide_ABC_transporter,_ATP-binding_protein_2031138..2031288_POSITIVE 405 115 71 75 22 aact:AA00218|3end_argininosuccinate_lyase_152211..152361_POSITIVE 400 100 63 146 105 aact:AA01775|3end_hypothetical_protein_1196182..1196332_POSITIVE 382 115 76 82 36 aact:AA01645|3end_ferric_transport_ABC-related_ATP-binding_protein_1115184..1115334_POSITIVE 360 115 76 54 13 aact:AA00010|3end_transposase_orfB_fragment_6297..6447_POSITIVE 326 118 72 113 48 aact:AA02142|3end_outer_membrane_protein_OprM_precursor_1467532..1467682_POSITIVE 323 88 43 66 15 aact:AA03006|3end_ribonuclease_III_2100167..2100317_POSITIVE 320 90 42 71 30 aact:AA00168|3end_possible_sigma_54_modulation_protein_120240..120390_POSITIVE 318 115 74 78 37 aact:AA02360|3end_threonine_synthase_1619947..1620097_POSITIVE 313 120 76 66 16 aact:AA00089|3end_S-adenosylmethionine_synthase;_methionine_adenosyltransferase_67749..67899_POSITIVE 312 115 75 79 34 aact:AA02777|3end_tRNA-guanine_transglycosylase_1942527..1942677_POSITIVE 312 120 74 72 23 aact:AA01963|3end_dnaK_suppressor_protein_1329438..1329588_POSITIVE 308 115 75 67 20 aact:AA02858|3end_conserved_hypothetical_protein_2006068..2006218_POSITIVE 303 120 74 79 31 aact:AA01083|3end_preprotein_translocase_SecA_subunit_750970..751120_POSITIVE 297 115 78 146 107 aact:AA00697|3end_hypothetical_protein_477174..477324_POSITIVE 297 115 78 78 39 aact:AA00696|3end_iron(III)_ABC_transporter,_periplasmic_iron-compound-binding_protein_477106..477256_POSITIVE 296 115 72 72 22 aact:AA02562|3end_adenine_specific_methylase_1785146..1785296_POSITIVE 295 115 76 121 77 aact:AA00495|3end_hypothetical_protein_345139..345289_POSITIVE 294 107 77 50 19 aact:AA02048|3end_galactitol_utilization_operon_repressor_1395579..1395729_POSITIVE 292 101 69 87 55 aact:AA02167|3end_hypothetical_protein_1483474..1483624_POSITIVE