Origin IGS: agatcctcaattatagaaggtagcaattaaaaaaatagaattaattgattgcgactatatcataattttacctataattcaccttatgtttagatattgcttaggcttttaacggcggtttctaaatgtgttctgcaaattttaataggagaaaaatc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gatttttctcctattaaaatttgcagaacacatttagaaaccgccgttaaaagcctaagcaatatctaaacataaggtgaattataggtaaaattatgatatagtcgcaatcaattaattctatttttttaattgctaccttctataattgaggatct Mask Tandem Repeat Region ================================================ agatcctcaattatagaaggtagcaattaaaaaaatagaattaattgattgcgactatatcataattttacctataattcaccttatgtttagatattgcttaggcttttaacggcggtttctaaatgtgttctgcaaattttaataggagaaaaatc Find is-nt database================================================ Query_seq: AA01513:AA01514|AA01513:AA01514:hydrogen peroxide-inducible activator:peroxiredoxin 2 family protein/glutaredoxin:->->:1019872..1020029 158 agatcctcaattatagaaggtagcaattaaaaaaatagaattaattgattgcgactatatcataattttacctataattcaccttatgtttagatattgcttaggcttttaacggcggtttctaaatgtgttctgcaaattttaataggagaaaaatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01513:AA01514|AA01513:AA01514:hydrogen peroxide-inducible activator:peroxiredoxin 2 family protein/glutaredoxin:->->:1019872..1020029 158 agatcctcaattatagaaggtagcaattaaaaaaatagaattaattgattgcgactatatcataattttacctataattcaccttatgtttagatattgcttaggcttttaacggcggtttctaaatgtgttctgcaaattttaataggagaaaaatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01513:AA01514|AA01513:AA01514:hydrogen peroxide-inducible activator:peroxiredoxin 2 family protein/glutaredoxin:->->:1019872..1020029 158 agatcctcaattatagaaggtagcaattaaaaaaatagaattaattgattgcgactatatcataattttacctataattcaccttatgtttagatattgcttaggcttttaacggcggtttctaaatgtgttctgcaaattttaataggagaaaaatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agatcctcaattatagaaggtagcaattaaaaaaatagaattaattgattgcgactatatcataattttacctataattcaccttatgtttagatattgcttaggcttttaacggcggtttctaaatgtgttctgcaaattttaataggagaaaaatc Predict ORF larger than 30AA ================================================ agatcctcaattatagaaggtagcaattaaaaaaatagaattaattgattgcgactatatcataattttacctataattcaccttatgtttagatattgcttaggcttttaacggcggtttctaaatgtgttctgcaaattttaataggagaaaaatc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agatcctcaattatagaaggtagcaattaaaaaaatagaattaattgattgcgactatatcataattttacctataattcaccttatgtttagatattgcttaggcttttaacggcggtttctaaatgtgttctgcaaattttaataggagaaaaatc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01513:AA01514|AA01513:AA01514:hydrogen peroxide-inducible activator:peroxiredoxin 2 family protein/glutaredoxin:->->:1019872..1020029 158 agatcctcaattatagaaggtagcaattaaaaaaatagaattaattgattgcgactatatcataattttacctataattcaccttatgtttagatattgcttaggcttttaacggcggtttctaaatgtgttctgcaaattttaataggagaaaaatc Intra-Species Hit: Count: 1 Min: 1 Max: 158 Len: 158 Subject: aact_AA01513_AA01514|hydrogen peroxide-inducible activator:peroxiredoxin 2 family protein/glutaredoxin|POSITIVE:POSITIVE|[1019872, 1020029]|158 HSP 1 e-value: 3.0E-72 bit: 272.0 Len: 158 Query Start:1 Query End:158 Subject Strand: POSITIVE Subject Start: 1 Subject End: 158 agatcctcaattatagaaggtagcaattnnnnnnntagaattaattgattgcgactatatcataattttacctataattcaccttatgtttagatattgcttaggcttttaacggcggtttctaaatgtgttctgcaaattttaataggagaaaaatc agatcctcaattatagaaggtagcaattaaaaaaatagaattaattgattgcgactatatcataattttacctataattcaccttatgtttagatattgcttaggcttttaacggcggtttctaaatgtgttctgcaaattttaataggagaaaaatc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAUCCUCAAUUAUAGAAGGUAGCAAUUAAAAAAAUAGAAUUAAUUGAUUGCGACUAUAUCAUAAUUUUACCUAUAAUUCACCUUAUGUUUAGAUAUUGCUUAGGCUUUUAACGGCGGUUUCUAAAUGUGUUCUGCAAAUUUUAAUAGGAGAAAAAUC ........((((((.((.(((.((((((((..............)))))))).)))...))))))))...(((((((((((...((((((((((.((((((..(........))))))).))))))))))...))..))))...)))))......... (-25.24) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUUUUUCUCCUAUUAAAAUUUGCAGAACACAUUUAGAAACCGCCGUUAAAAGCCUAAGCAAUAUCUAAACAUAAGGUGAAUUAUAGGUAAAAUUAUGAUAUAGUCGCAAUCAAUUAAUUCUAUUUUUUUAAUUGCUACCUUCUAUAAUUGAGGAUCU (((((((..(((((...((((..(........((((((....((.((.....))....))....))))))......)..)))))))))...((((((((....((.(((((.((..((......))..)).))))).))..)).))))))))))))). (-19.64) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 309 146 102 82 21 aact:AA00191|5end_conserved_hypothetical_protein_(possible_acetyltransferase,_GNAT_family)_135019..135249_POSITIVE 297 138 98 67 27 aact:AA01166|5end_sulphite_synthesis_pathway_protein_798599..798829_POSITIVE 291 149 103 143 94 aact:AA02450|5end_conserved_hypothetical_protein_1687701..1687931_POSITIVE 284 116 71 146 99 aact:AA01367|5end_tRNA_(Guanine-N(1)-)-methyltransferase_(M1G-methyltransferase)_923710..923940_POSITIVE 272 137 98 227 182 aact:AA00341|5end_hypothetical_protein_229388..229618_POSITIVE 268 136 101 66 27 aact:AA01974|5end_conserved_hypothetical_protein_1335891..1336121_POSITIVE 261 138 92 88 47 aact:AA01995|5end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355120..1355350_POSITIVE 258 136 92 224 180 aact:AA01083|5end_preprotein_translocase_SecA_subunit_748131..748361_POSITIVE 255 144 104 223 178 aact:AA01838|5end_conserved_hypothetical_protein_1236396..1236626_POSITIVE 254 136 96 35 2 aact:AA01700|5end_hexulose-6-phosphate_isomerase_1154535..1154765_POSITIVE 251 136 94 210 161 aact:AA02187|5end_hypothetical_protein_1492603..1492833_POSITIVE 250 140 96 110 69 aact:AA02623|5end_dTDP-D-glucose_4,6-dehydratase_1827300..1827530_POSITIVE 246 136 97 47 1 aact:AA00237|5end_hypothetical_protein_170934..171164_POSITIVE 245 136 92 79 36 aact:AA02549|5end_iron_(chelated)_ABC_transporter,_periplasmic-binding_protein_1775870..1776100_POSITIVE 243 140 92 78 34 aact:AA02470|5end_glucose_kinase_1706730..1706960_POSITIVE 243 117 79 166 125 aact:AA00989|5end_hypothetical_protein_692319..692549_POSITIVE 241 118 71 61 6 aact:AA02419|5end_hypothetical_protein_1661731..1661961_POSITIVE 241 136 91 73 29 aact:AA01928|5end_ATP-dependent_RNA_helicase_1301986..1302216_POSITIVE 240 130 81 161 98 aact:AA01089|5end_hypothetical_protein_753392..753622_POSITIVE 240 122 87 167 125 aact:AA00636|5end_MrsA_protein_(phosphoglucomutase/phosphomannomutase)_436780..437010_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 284 116 71 77 30 aact:AA01366|3end_16S_rRNA_processing_protein_923721..923871_POSITIVE 265 136 92 54 2 aact:AA00236|3end_anaerobic_ribonucleoside-triphosphate_reductase_171021..171171_POSITIVE 261 138 92 139 98 aact:AA01994|3end_chorismate_mutase;_prephenate_dehydrogenase_1355251..1355401_POSITIVE 257 124 89 134 94 aact:AA01069|3end_translation_elongation_factor_(EF-Ts)_740700..740850_POSITIVE 255 144 104 143 98 aact:AA01837|3end_transcriptional_regulatory_protein_1236396..1236546_POSITIVE 251 136 94 68 19 aact:AA02184|3end_anthranilate_isomerase;_indole-3-glycerol_phosphate_synthase;_phosphoribosylanthranilate_isomerase_1492541..1492691_POSITIVE 243 140 92 65 21 aact:AA02468|3end_conserved_hypothetical_protein_1706797..1706947_POSITIVE 241 118 71 61 6 aact:AA02418|3end_peptidyl-tRNA_hydrolase_1661811..1661961_POSITIVE 240 122 87 135 93 aact:AA00635|3end_dihydropteroate_synthase_436828..436978_POSITIVE 238 117 71 118 67 aact:AA00569|3end_ketol-acid_reductoisomerase_391582..391732_POSITIVE 236 117 76 42 1 aact:AA01419|3end_D-ribulose-phosphate-3_epimerase_955129..955279_POSITIVE 235 140 98 46 6 aact:AA02043|3end_tagatose_6-phosphate_kinase_1391587..1391737_POSITIVE 231 136 97 98 57 aact:AA01925|3end_conserved_hypothetical_protein_1301000..1301150_POSITIVE 227 138 114 26 3 aact:AA01165|3end_conserved_hypothetical_protein_798638..798788_POSITIVE 226 134 96 76 35 aact:AA00748|3end_hypothetical_protein_513826..513976_POSITIVE 225 119 83 126 87 aact:AA01199|3end_conserved_hypothetical_protein_822486..822636_POSITIVE 223 137 98 124 84 aact:AA02802|3end_probable_myo-inositol_2-dehydrogenase_1970086..1970236_POSITIVE 223 127 83 102 45 aact:AA01331|3end_50S_ribosomal_protein_L31_898721..898871_POSITIVE 221 149 103 80 35 aact:AA02019|3end_hypothetical_protein_1373925..1374075_POSITIVE 216 137 92 141 103 aact:AA02885|3end_conserved_hypothetical_protein_2023422..2023572_POSITIVE