Origin IGS: tagttgttcctacaaagtgcggctagcttaactatttgaggggtataaaacctaaagatctgtttttggcatgaaatgtaaaataagcagagctaaattgaatctgttaaaaaatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taattttttaacagattcaatttagctctgcttattttacatttcatgccaaaaacagatctttaggttttatacccctcaaatagttaagctagccgcactttgtaggaacaacta Mask Tandem Repeat Region ================================================ tagttgttcctacaaagtgcggctagcttaactatttgaggggtataaaacctaaagatctgtttttggcatgaaatgtaaaataagcagagctaaattgaatctgttaaaaaatta Find is-nt database================================================ Query_seq: AA01510:AA01511|AA01510:AA01511:ABC transporter, ATP-binding protein/permease:probable transcriptional regulator:->->:1018226..1018342 117 tagttgttcctacaaagtgcggctagcttaactatttgaggggtataaaacctaaagatctgtttttggcatgaaatgtaaaataagcagagctaaattgaatctgttaaaaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01510:AA01511|AA01510:AA01511:ABC transporter, ATP-binding protein/permease:probable transcriptional regulator:->->:1018226..1018342 117 tagttgttcctacaaagtgcggctagcttaactatttgaggggtataaaacctaaagatctgtttttggcatgaaatgtaaaataagcagagctaaattgaatctgttaaaaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01510:AA01511|AA01510:AA01511:ABC transporter, ATP-binding protein/permease:probable transcriptional regulator:->->:1018226..1018342 117 tagttgttcctacaaagtgcggctagcttaactatttgaggggtataaaacctaaagatctgtttttggcatgaaatgtaaaataagcagagctaaattgaatctgttaaaaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagttgttcctacaaagtgcggctagcttaactatttgaggggtataaaacctaaagatctgtttttggcatgaaatgtaaaataagcagagctaaattgaatctgttaaaaaatta Predict ORF larger than 30AA ================================================ tagttgttcctacaaagtgcggctagcttaactatttgaggggtataaaacctaaagatctgtttttggcatgaaatgtaaaataagcagagctaaattgaatctgttaaaaaatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagttgttcctacaaagtgcggctagcttaactatttgaggggtataaaacctaaagatctgtttttggcatgaaatgtaaaataagcagagctaaattgaatctgttaaaaaatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01510:AA01511|AA01510:AA01511:ABC transporter, ATP-binding protein/permease:probable transcriptional regulator:->->:1018226..1018342 117 tagttgttcctacaaagtgcggctagcttaactatttgaggggtataaaacctaaagatctgtttttggcatgaaatgtaaaataagcagagctaaattgaatctgttaaaaaatta Intra-Species Hit: Count: 1 Min: 1 Max: 117 Len: 117 Subject: aact_AA01510_AA01511|ABC transporter, ATP-binding protein/permease:probable transcriptional regulator|POSITIVE:POSITIVE|[1018226,1018342]|117 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 117 Query Start:1 Query End:117 Subject Strand: POSITIVE Subject Start: 1 Subject End: 117 tagttgttcctacaaagtgcggctagcttaactatttgaggggtataaaacctaaagatctgtttttggcatgaaatgtaaaataagcagagctaaattgaatctgttaaaaaatta tagttgttcctacaaagtgcggctagcttaactatttgaggggtataaaacctaaagatctgtttttggcatgaaatgtaaaataagcagagctaaattgaatctgttaaaaaatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUUGUUCCUACAAAGUGCGGCUAGCUUAACUAUUUGAGGGGUAUAAAACCUAAAGAUCUGUUUUUGGCAUGAAAUGUAAAAUAAGCAGAGCUAAAUUGAAUCUGUUAAAAAAUUA (((((((..((....)).)))))))..(((((.(((..(.((((.....))))..((.(((((((...((((...)))).....))))))).))...)..)))..)))))....... (-21.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUUUUUAACAGAUUCAAUUUAGCUCUGCUUAUUUUACAUUUCAUGCCAAAAACAGAUCUUUAGGUUUUAUACCCCUCAAAUAGUUAAGCUAGCCGCACUUUGUAGGAACAACUA .....(((..(((((......((((((..(((.((((..........(((.(((.......))).)))............)))))))..))))))......)))))..)))...... (-12.55) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 286 29 6 163 140 aact:AA00464|5end_conserved_hypothetical_protein_323912..324142_POSITIVE 284 45 1 181 129 aact:AA00131|5end_transcriptional_regulator_(MerR_family)_94521..94751_POSITIVE 248 97 57 211 168 aact:AA01265|5end_hypothetical_protein_861979..862209_POSITIVE 248 97 57 95 52 aact:AA01264|5end_conserved_hypothetical_protein_861863..862093_POSITIVE 245 106 61 70 24 aact:AA02080|5end_ABC_transporter,_ATP-binding/permease_1412744..1412974_POSITIVE 244 44 3 164 123 aact:AA01661|5end_molybdopterin_biosynthesis_protein_1125384..1125614_POSITIVE 237 80 37 99 51 aact:AA01376|5end_hypothetical_protein_931163..931393_POSITIVE 232 44 6 187 149 aact:AA02099|5end_50S_ribosomal_protein_L25_1429639..1429869_POSITIVE 232 22 2 213 188 aact:AA00698|5end_iron(III)_ABC_transporter,_periplasmic_iron-compound-binding_protein_477191..477421_POSITIVE 228 60 12 106 55 aact:AA00694|5end_dCTP_deaminase_(deoxycytidine_triphosphate_deaminas)_474439..474669_POSITIVE 228 50 2 209 158 aact:AA00675|5end_glutaredoxin_2_462524..462754_POSITIVE 226 46 2 186 134 aact:AA01878|5end_hydroxyacylglutathione_hydrolase_1264831..1265061_POSITIVE 223 46 4 151 93 aact:AA01601|5end_PTS_system,_glucose-specific_IIA_componen_1082269..1082499_POSITIVE 222 69 21 229 174 aact:AA01136|5end_hypothetical_protein_778285..778515_POSITIVE 220 55 16 47 15 aact:AA00383|5end_chloride_channel_protein_258894..259124_POSITIVE 219 47 3 85 47 aact:AA00814|5end_conserved_hypothetical_protein_559125..559355_POSITIVE 217 42 3 201 157 aact:AA02649|5end_possible_glycosyltransferase_1845562..1845792_POSITIVE 216 68 22 174 133 aact:AA01920|5end_N_utilization_substance_protein_A_1293198..1293428_POSITIVE 215 80 38 105 74 aact:AA02721|5end_spermidine/putrescine_ABC_transporter,_periplasmic-binding_protein_1904121..1904351_POSITIVE 214 78 35 150 114 aact:AA02357|5end_bifunctional_protein:_aspartokinase_I;_homoserine_dehydrogenaseI_1614668..1614898_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 286 29 6 69 46 aact:AA00463|3end_class_B_acid_phosphatase_323898..324048_POSITIVE 284 45 1 101 49 aact:AA00130|3end_mercuric_transport_protein_(periplasmic_mercury_ion_binding_protein)_(mercury_scavenger_protein)_94521..94671_POSITIVE 284 45 1 132 80 aact:AA00129|3end_hypothetical_protein_94552..94702_POSITIVE 252 109 67 58 18 aact:AA01984|3end_tellurite_resistance_protein;_hemagglutinin_homolog_1345754..1345904_POSITIVE 237 80 37 60 12 aact:AA01375|3end_hypothetical_protein_931204..931354_POSITIVE 235 63 26 147 106 aact:AA02528|3end_30S_ribosomal_protein_S1_1757692..1757842_POSITIVE 232 22 2 116 91 aact:AA00697|3end_hypothetical_protein_477174..477324_POSITIVE 232 22 2 48 23 aact:AA00696|3end_iron(III)_ABC_transporter,_periplasmic_iron-compound-binding_protein_477106..477256_POSITIVE 228 60 12 102 51 aact:AA00692|3end_conserved_hypothetical_protein_474515..474665_POSITIVE 223 46 4 87 29 aact:AA01600|3end_phosphotransferase_system_enzyme_I_1082285..1082435_POSITIVE 216 68 22 147 106 aact:AA01919|3end_conserved_hypothetical_protein_1293251..1293401_POSITIVE 216 46 9 123 77 aact:AA00172|3end_conserved_hypothetical_protein_(possible_regulator_protein_/_nitrogen_fixation_protein)_122904..123054_POSITIVE 214 78 35 58 22 aact:AA02356|3end_hypothetical_protein_1614656..1614806_POSITIVE 214 48 3 54 7 aact:AA00075|3end_conserved_hypothetical_protein_55636..55786_POSITIVE 212 43 1 55 8 aact:AA00565|3end_30S_ribosomal_protein_S7_390519..390669_POSITIVE 211 44 3 52 14 aact:AA02910|3end_ribonuclease_E_2039105..2039255_POSITIVE 211 45 1 151 109 aact:AA02814|3end_hypothetical_protein_1980445..1980595_POSITIVE 211 76 37 79 37 aact:AA01238|3end_50S_ribosomal_protein_L36_844438..844588_POSITIVE 210 88 41 68 9 aact:AA02630|3end_hypothetical_protein_1830474..1830624_POSITIVE 208 43 3 85 29 aact:AA02137|3end_transcriptional_regulators_(TetR/AcrR_family)_1461093..1461243_POSITIVE