Origin IGS: taacacgattttgcttcggggaataatagcgtcttatttcaggcgctatttttttatttaaccgttaaacacgaaatatattatttttacgaataaaattaattagcatttgtcatataaatgttatgatatagcactttcattgaggtgatttaaggacagataaacattatg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 cataatgtttatctgtccttaaatcacctcaatgaaagtgctatatcataacatttatatgacaaatgctaattaattttattcgtaaaaataatatatttcgtgtttaacggttaaataaaaaaatagcgcctgaaataagacgctattattccccgaagcaaaatcgtgtta Mask Tandem Repeat Region ================================================ taacacgattttgcttcggggaataatagcgtcttatttcaggcgctatttttttatttaaccgttaaacacgaaatatattatttttacgaataaaattaattagcatttgtcatataaatgttatgatatagcactttcattgaggtgatttaaggacagataaacattatg Find is-nt database================================================ Query_seq: AA01508:AA01509|AA01508:AA01509:anaerobic C4-dicarboxylate transporter:ABC system transport protein, ATP binding/permease:->->:1014658..1014831 174 taacacgattttgcttcggggaataatagcgtcttatttcaggcgctatttttttatttaaccgttaaacacgaaatatattatttttacgaataaaattaattagcatttgtcatataaatgttatgatatagcactttcattgaggtgatttaaggacagataaacattatg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01508:AA01509|AA01508:AA01509:anaerobic C4-dicarboxylate transporter:ABC system transport protein, ATP binding/permease:->->:1014658..1014831 174 taacacgattttgcttcggggaataatagcgtcttatttcaggcgctatttttttatttaaccgttaaacacgaaatatattatttttacgaataaaattaattagcatttgtcatataaatgttatgatatagcactttcattgaggtgatttaaggacagataaacattatg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01508:AA01509|AA01508:AA01509:anaerobic C4-dicarboxylate transporter:ABC system transport protein, ATP binding/permease:->->:1014658..1014831 174 taacacgattttgcttcggggaataatagcgtcttatttcaggcgctatttttttatttaaccgttaaacacgaaatatattatttttacgaataaaattaattagcatttgtcatataaatgttatgatatagcactttcattgaggtgatttaaggacagataaacattatg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacacgattttgcttcggggaataatagcgtcttatttcaggcgctatttttttatttaaccgttaaacacgaaatatattatttttacgaataaaattaattagcatttgtcatataaatgttatgatatagcactttcattgaggtgatttaaggacagataaacattatg Predict ORF larger than 30AA ================================================ taacacgattttgcttcggggaataatagcgtcttatttcaggcgctatttttttatttaaccgttaaacacgaaatatattatttttacgaataaaattaattagcatttgtcatataaatgttatgatatagcactttcattgaggtgatttaaggacagataaacattatg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacacgattttgcttcggggaataatagcgtcttatttcaggcgctatttttttatttaaccgttaaacacgaaatatattatttttacgaataaaattaattagcatttgtcatataaatgttatgatatagcactttcattgaggtgatttaaggacagataaacattatg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01508:AA01509|AA01508:AA01509:anaerobic C4-dicarboxylate transporter:ABC system transport protein, ATP binding/permease:->->:1014658..1014831 174 taacacgattttgcttcggggaataatagcgtcttatttcaggcgctatttttttatttaaccgttaaacacgaaatatattatttttacgaataaaattaattagcatttgtcatataaatgttatgatatagcactttcattgaggtgatttaaggacagataaacattatg Intra-Species Hit: Count: 1 Min: 1 Max: 174 Len: 174 Subject: aact_AA01508_AA01509|anaerobic C4-dicarboxylate transporter:ABC system transport protein, ATP binding/permease|POSITIVE:POSITIVE|[1014658,1014831]|174 HSP 1 e-value: 1.0E-81 bit: 303.0 Len: 174 Query Start:1 Query End:174 Subject Strand: POSITIVE Subject Start: 1 Subject End: 174 taacacgattttgcttcggggaataatagcgtcttatttcaggcgctannnnnnnatttaaccgttaaacacgaaatatattatttttacgaataaaattaattagcatttgtcatataaatgttatgatatagcactttcattgaggtgatttaaggacagataaacattatg taacacgattttgcttcggggaataatagcgtcttatttcaggcgctatttttttatttaaccgttaaacacgaaatatattatttttacgaataaaattaattagcatttgtcatataaatgttatgatatagcactttcattgaggtgatttaaggacagataaacattatg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACACGAUUUUGCUUCGGGGAAUAAUAGCGUCUUAUUUCAGGCGCUAUUUUUUUAUUUAACCGUUAAACACGAAAUAUAUUAUUUUUACGAAUAAAAUUAAUUAGCAUUUGUCAUAUAAAUGUUAUGAUAUAGCACUUUCAUUGAGGUGAUUUAAGGACAGAUAAACAUUAUG .........(((.(....).))).((((((((((......))))))))))..(((((((..((.((((((((........((((((....)))))).........((...(((((((.......)))))))..))............))).)))))))..)))))))....... (-32.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAUAAUGUUUAUCUGUCCUUAAAUCACCUCAAUGAAAGUGCUAUAUCAUAACAUUUAUAUGACAAAUGCUAAUUAAUUUUAUUCGUAAAAAUAAUAUAUUUCGUGUUUAACGGUUAAAUAAAAAAAUAGCGCCUGAAAUAAGACGCUAUUAUUCCCCGAAGCAAAAUCGUGUUA .....((((((.((((...((((.(((............((....(((((.......))))).....))..((((.(((((....))))).)))).......))))))))))).))))))....(((((((.((......)).))))))).........((((......)))). (-23.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 317 46 6 191 150 aact:AA01107|5end_adenylosuccinate_lyase_762310..762540_POSITIVE 306 50 5 84 24 aact:AA02338|5end_conserved_hypothetical_protein;_possible_integral_membrane_protein_1598083..1598313_POSITIVE 283 48 3 105 63 aact:AA01745|5end_conserved_hypothetical_protein_(possible_peptide_chain_release_factor_2)_1180394..1180624_POSITIVE 266 45 6 53 11 aact:AA01056|5end_conserved_hypothetical_protein_733867..734097_POSITIVE 266 57 14 157 120 aact:AA00826|5end_UDP-3-O-acyl-GlcNAc_deacetylase_568378..568608_POSITIVE 263 47 6 198 158 aact:AA02365|5end_hflC_protein_1621997..1622227_POSITIVE 262 46 4 179 133 aact:AA01519|5end_conserved_hypothetical_protein_1021794..1022024_POSITIVE 259 47 4 71 25 aact:AA02325|5end_membrane_protein;_possible_ABC_transporter,_permease_1587309..1587539_POSITIVE 257 46 7 45 11 aact:AA01349|5end_NAD(P)H_nitroreductase/H-flavin_oxidoreductase_913619..913849_POSITIVE 255 55 11 133 77 aact:AA02154|5end_possible_ATP-binding_protein_of_ABC_transporter_1473543..1473773_POSITIVE 254 45 1 104 60 aact:AA02990|5end_beta_hexosaminadase_2089473..2089703_POSITIVE 249 46 4 149 107 aact:AA00047|5end_formate_dependent_nitrite_reductase,_cytochrome_c552_36753..36983_POSITIVE 248 56 15 153 95 aact:AA00306|5end_nitrogen_regulatory_protein_p-II_207923..208153_POSITIVE 247 45 2 68 13 aact:AA01370|5end_rod_shape-determining_protein_925436..925666_POSITIVE 246 45 6 177 132 aact:AA00500|5end_hypothetical_protein_347151..347381_POSITIVE 242 66 25 190 145 aact:AA01277|5end_aspartate_ammonia-lyase_865949..866179_POSITIVE 239 46 1 91 58 aact:AA02889|5end_hypothetical_protein_2025045..2025275_POSITIVE 239 49 4 128 79 aact:AA02342|5end_ISRSO11/IS150_related_transposase,_orfB_1599565..1599795_POSITIVE 239 49 4 128 79 aact:AA02117|5end_probable_transposase_1445207..1445437_POSITIVE 239 49 4 128 79 aact:AA01077|5end_transposase_746284..746514_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 266 45 6 46 4 aact:AA01054|3end_conserved_hypothetical_protein_733940..734090_POSITIVE 262 46 4 96 50 aact:AA01517|3end_peptidyl-prolyl_cis-trans_isomerase;_macrophage_infectivity_protein_1021791..1021941_POSITIVE 259 47 4 74 28 aact:AA02324|3end_ABC_transporter_ATP_binding_protein_1587392..1587542_POSITIVE 257 46 7 45 11 aact:AA01348|3end_hypothetical_protein_913699..913849_POSITIVE 255 55 11 130 74 aact:AA02153|3end_iron(III)_ABC_transporter,_permease_protein_1473620..1473770_POSITIVE 254 45 1 99 55 aact:AA02989|3end_conserved_hypothetical_protein_2089548..2089698_POSITIVE 249 46 4 72 30 aact:AA00046|3end_formate_dependent_nitrite_reductase,_cytochrome-c_type_protein_36756..36906_POSITIVE 247 45 2 65 10 aact:AA01369|3end_rod_shape-determining_protein_925513..925663_POSITIVE 246 41 5 149 106 aact:AA00072|3end_conserved_hypothetical_protein_54947..55097_POSITIVE 239 49 4 131 82 aact:AA02341|3end_hypothetical_protein_1599648..1599798_POSITIVE 239 49 4 131 82 aact:AA02116|3end_hypothetical_protein_1445290..1445440_POSITIVE 239 49 4 131 82 aact:AA01076|3end_hypothetical_protein_746367..746517_POSITIVE 239 49 4 131 82 aact:AA00009|3end_hypothetical_protein_(possible_transposase_fragment)_5794..5944_POSITIVE 238 44 3 129 96 aact:AA02480|3end_excinuclease_ABC_subunit_B_1718442..1718592_POSITIVE 238 45 6 129 84 aact:AA01751|3end_conserved_hypothetical_protein_1185392..1185542_POSITIVE 238 30 6 148 123 aact:AA01106|3end_conserved_hypothetical_protein_762363..762513_POSITIVE 237 50 8 66 14 aact:AA01508|3end_anaerobic_C4-dicarboxylate_transporter_1014597..1014747_POSITIVE 235 48 10 145 94 aact:AA01428|3end_acetolactate_synthase_959625..959775_POSITIVE 235 59 17 144 100 aact:AA01194|3end_acyl_carrier_protein_819923..820073_POSITIVE 235 43 1 83 51 aact:AA01152|3end_sec-independent_protein_translocase_protein_790205..790355_POSITIVE