Origin IGS: ttatcaaaaattattgtctctgcagattctattaattttgttgaaataacaatcaattatttatttaaagtgtgatatagatcataatttaatcagaacgtccaaaatccgccatactcaccattacaacattcttatataattaaggtgaattttgaagttaactaaatcccgattgcctttgtataaggcatttctctcaaaatctcattgagtataatctcttatacattaaacaattttttatttttattctaaggaggcagt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 actgcctccttagaataaaaataaaaaattgtttaatgtataagagattatactcaatgagattttgagagaaatgccttatacaaaggcaatcgggatttagttaacttcaaaattcaccttaattatataagaatgttgtaatggtgagtatggcggattttggacgttctgattaaattatgatctatatcacactttaaataaataattgattgttatttcaacaaaattaatagaatctgcagagacaataatttttgataa Mask Tandem Repeat Region ================================================ ttatcaaaaattattgtctctgcagattctattaattttgttgaaataacaatcaattatttatttaaagtgtgatatagatcataatttaatcagaacgtccaaaatccgccatactcaccattacaacattcttatataattaaggtgaattttgaagttaactaaatcccgattgcctttgtataaggcatttctctcaaaatctcattgagtataatctcttatacattaaacaattttttatttttattctaaggaggcagt Find is-nt database================================================ Query_seq: AA01507:AA01508|AA01507:AA01508:conserved hypothetical protein:anaerobic C4-dicarboxylate transporter:->->:1013071..1013337 267 ttatcaaaaattattgtctctgcagattctattaattttgttgaaataacaatcaattatttatttaaagtgtgatatagatcataatttaatcagaacgtccaaaatccgccatactcaccattacaacattcttatataattaaggtgaattttgaagttaactaaatcccgattgcctttgtataaggcatttctctcaaaatctcattgagtataatctcttatacattaaacaattttttatttttattctaaggaggcagt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01507:AA01508|AA01507:AA01508:conserved hypothetical protein:anaerobic C4-dicarboxylate transporter:->->:1013071..1013337 267 ttatcaaaaattattgtctctgcagattctattaattttgttgaaataacaatcaattatttatttaaagtgtgatatagatcataatttaatcagaacgtccaaaatccgccatactcaccattacaacattcttatataattaaggtgaattttgaagttaactaaatcccgattgcctttgtataaggcatttctctcaaaatctcattgagtataatctcttatacattaaacaattttttatttttattctaaggaggcagt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01507:AA01508|AA01507:AA01508:conserved hypothetical protein:anaerobic C4-dicarboxylate transporter:->->:1013071..1013337 267 ttatcaaaaattattgtctctgcagattctattaattttgttgaaataacaatcaattatttatttaaagtgtgatatagatcataatttaatcagaacgtccaaaatccgccatactcaccattacaacattcttatataattaaggtgaattttgaagttaactaaatcccgattgcctttgtataaggcatttctctcaaaatctcattgagtataatctcttatacattaaacaattttttatttttattctaaggaggcagt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttatcaaaaattattgtctctgcagattctattaattttgttgaaataacaatcaattatttatttaaagtgtgatatagatcataatttaatcagaacgtccaaaatccgccatactcaccattacaacattcttatataattaaggtgaattttgaagttaactaaatcccgattgcctttgtataaggcatttctctcaaaatctcattgagtataatctcttatacattaaacaattttttatttttattctaaggaggcagt Predict ORF larger than 30AA ================================================ ttatcaaaaattattgtctctgcagattctattaattttgttgaaataacaatcaattatttatttaaagtgtgatatagatcataatttaatcagaacgtccaaaatccgccatactcaccattacaacattcttatataattaaggtgaattttgaagttaactaaatcccgattgcctttgtataaggcatttctctcaaaatctcattgagtataatctcttatacattaaacaattttttatttttattctaaggaggcagt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttatcaaaaattattgtctctgcagattctattaattttgttgaaataacaatcaattatttatttaaagtgtgatatagatcataatttaatcagaacgtccaaaatccgccatactcaccattacaacattcttatataattaaggtgaattttgaagttaactaaatcccgattgcctttgtataaggcatttctctcaaaatctcattgagtataatctcttatacattaaacaattttttatttttattctaaggaggcagt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 70 HP_score: -6.6 Tail_Score: -4.04474 Start: 177 End: 193 Full_Region: TAACTAAATCCCGAT TGCCTT TGTAT AAGGCA TTTCTCTCAAAATCT ................................................................................................................................................................................TGCCTTTGTATAAGGCA.......................................................................... TransTerm Strand: + Conf: 40 HP_score: -2.3 Tail_Score: -2.93633 Start: 199 End: 215 Full_Region: GTATAAGGCATTTCT CTCAA AATCTCA TTGAG TATAATCTCTTATAC ......................................................................................................................................................................................................CTCAAAATCTCATTGAG.................................................... Find igs database================================================ Query_seq: AA01507:AA01508|AA01507:AA01508:conserved hypothetical protein:anaerobic C4-dicarboxylate transporter:->->:1013071..1013337 267 ttatcaaaaattattgtctctgcagattctattaattttgttgaaataacaatcaattatttatttaaagtgtgatatagatcataatttaatcagaacgtccaaaatccgccatactcaccattacaacattcttatataattaaggtgaattttgaagttaactaaatcccgattgcctttgtataaggcatttctctcaaaatctcattgagtataatctcttatacattaaacaattttttatttttattctaaggaggcagt Intra-Species Hit: Count: 1 Min: 1 Max: 239 Len: 239 Subject: aact_AA01507_AA01508|conserved hypothetical protein:anaerobic C4-dicarboxylate transporter|POSITIVE:POSITIVE|[1013071,1013337]|267 HSP 1 e-value: 1.0E-133 bit: 474.0 Len: 239 Query Start:1 Query End:239 Subject Strand: POSITIVE Subject Start: 1 Subject End: 239 ttatcaaaaattattgtctctgcagattctattaattttgttgaaataacaatcaattatttatttaaagtgtgatatagatcataatttaatcagaacgtccaaaatccgccatactcaccattacaacattcttatataattaaggtgaattttgaagttaactaaatcccgattgcctttgtataaggcatttctctcaaaatctcattgagtataatctcttatacattaaacaa............................ ttatcaaaaattattgtctctgcagattctattaattttgttgaaataacaatcaattatttatttaaagtgtgatatagatcataatttaatcagaacgtccaaaatccgccatactcaccattacaacattcttatataattaaggtgaattttgaagttaactaaatcccgattgcctttgtataaggcatttctctcaaaatctcattgagtataatctcttatacattaaacaa............................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAUCAAAAAUUAUUGUCUCUGCAGAUUCUAUUAAUUUUGUUGAAAUAACAAUCAAUUAUUUAUUUAAAGUGUGAUAUAGAUCAUAAUUUAAUCAGAACGUCCAAAAUCCGCCAUACUCACCAUUACAACAUUCUUAUAUAAUUAAGGUGAAUUUUGAAGUUAACUAAAUCCCGAUUGCCUUUGUAUAAGGCAUUUCUCUCAAAAUCUCAUUGAGUAUAAUCUCUUAUACAUUAAACAA ...((((((((((..((((....)))).....))))))..)))).....(((((..........(((((.((((((....)))))).)))))....(((...((((((.((((.................................)))).))))))..)))...........)))))....(((((((((.(((...(((((.......)))))...))).)))))))))........ (-36.31) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGUUUAAUGUAUAAGAGAUUAUACUCAAUGAGAUUUUGAGAGAAAUGCCUUAUACAAAGGCAAUCGGGAUUUAGUUAACUUCAAAAUUCACCUUAAUUAUAUAAGAAUGUUGUAAUGGUGAGUAUGGCGGAUUUUGGACGUUCUGAUUAAAUUAUGAUCUAUAUCACACUUUAAAUAAAUAAUUGAUUGUUAUUUCAACAAAAUUAAUAGAAUCUGCAGAGACAAUAAUUUUUGAUAA ..........(((.(((((((((.(((....(((((((((......((((((.....)))))).))))))))).............((((((((((...((((....))))..))).)))))))...((((((((((...(((.((.(((((.(.((((....)))).).)))))..(((((((.....)))))))...)).)))...)))))))))).)))...))))))))).))). (-43.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 316 135 94 135 85 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 276 127 101 227 199 aact:AA01692|5end_hexulose-6-phosphate_synthase_1149178..1149408_POSITIVE 260 128 88 95 53 aact:AA02076|5end_conserved_hypothetical_protein_1409926..1410156_POSITIVE 254 136 94 214 176 aact:AA03014|5end_conserved_hypothetical_protein_2104815..2105045_POSITIVE 250 203 164 217 180 aact:AA01457|5end_D-xylose_ABC_transporter,_membrane_spanning_protein_977604..977834_POSITIVE 248 215 172 156 109 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 241 219 173 125 79 aact:AA01782|5end_molybdenum_transport_protein;_transcriptional_regulator_1202879..1203109_POSITIVE 238 217 171 204 162 aact:AA01646|5end_hypothetical_protein_1115069..1115299_POSITIVE 237 133 103 205 170 aact:AA01255|5end_hypothetical_protein_852656..852886_POSITIVE 237 213 171 81 33 aact:AA01159|5end_phosphatase_792828..793058_POSITIVE 231 209 169 59 17 aact:AA01954|5end_excinuclease_ABC,_subunit_C_1320468..1320698_POSITIVE 228 179 147 103 74 aact:AA00785|5end_conserved_hypothetical_protein_(possible_integrase)_539221..539451_POSITIVE 226 126 95 168 135 aact:AA01883|5end_recycling_protein;_permease_1269821..1270051_POSITIVE 225 207 170 196 159 aact:AA01502|5end_serine_acetyltransferase_1010014..1010244_POSITIVE 224 133 96 91 44 aact:AA01303|5end_competence_protein_A_881996..882226_POSITIVE 222 150 109 47 8 aact:AA01732|5end_multidrug_resistance_protein_A_1170677..1170907_POSITIVE 222 131 91 225 189 aact:AA01238|5end_50S_ribosomal_protein_L36_844248..844478_POSITIVE 221 139 95 185 139 aact:AA02409|5end_DNA_ligase_1653315..1653545_POSITIVE 219 213 173 185 139 aact:AA02195|5end_oxaloacetate_decarboxylase_alpha_chain_1494501..1494731_POSITIVE 219 157 111 145 95 aact:AA00900|5end_conserved_hypothetical_protein_623442..623672_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 298 131 98 95 63 aact:AA02697|3end_hydrogenase_3_maturation_protease_1880408..1880558_POSITIVE 276 127 101 111 83 aact:AA01690|3end_penicillin-binding_protein_1C_1149142..1149292_POSITIVE 260 128 88 68 26 aact:AA02075|3end_conserved_hypothetical_protein_1409979..1410129_POSITIVE 248 215 172 95 48 aact:AA01289|3end_hypothetical_protein_872848..872998_POSITIVE 237 213 171 65 17 aact:AA01158|3end_ribosome_associated_heat_shock_protein_15_homolog_792892..793042_POSITIVE 235 122 81 129 90 aact:AA02254|3end_2-oxoglutarate/malate_translocator_1536380..1536530_POSITIVE 228 179 147 113 84 aact:AA00784|3end_hypothetical_protein_539311..539461_POSITIVE 225 207 170 142 105 aact:AA01501|3end_hypothetical_protein_1010040..1010190_POSITIVE 224 133 96 110 63 aact:AA01301|3end_competence_protein_B_882095..882245_POSITIVE 219 157 111 102 52 aact:AA00899|3end_possible_quaternary_ammonium_compound_resistance_protein_623479..623629_POSITIVE 217 122 96 44 12 aact:AA01859|3end_hypothetical_protein_1251890..1252040_POSITIVE 216 94 51 75 27 aact:AA00317|3end_DNA_mismatch_repair_protein_218110..218260_POSITIVE 215 205 170 48 4 aact:AA01005|3end_conserved_hypothetical_protein_702198..702348_POSITIVE 214 105 67 108 71 aact:AA01270|3end_hydrolase_(possible_2-haloalkanoic_acid_dehalogenase_I)_863897..864047_POSITIVE 210 113 71 148 106 aact:AA00993|3end_possible_D-tyrosyl-tRNA(Tyr)_deacylase_694999..695149_POSITIVE 209 128 91 151 100 aact:AA01062|3end_ribonuclease_PH_(RNase_PH)_736499..736649_POSITIVE 208 114 73 113 79 aact:AA01757|3end_cobalt_transport_ATP-binding_protein_1187837..1187987_POSITIVE 208 193 167 133 110 aact:AA01608|3end_conserved_hypothetical_protein_1087977..1088127_POSITIVE 207 126 97 32 1 aact:AA01881|3end_adenylate_kinase_1269765..1269915_POSITIVE 207 134 95 133 96 aact:AA01357|3end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919785..919935_POSITIVE