Origin IGS: taattataaaaataaagccggcaattaaaccggcttttatttattttactttgagtgaatatcgttttttaaataatttcggcgaataactcatatttccatagattccccattgaaggtctatataaatgtcaccctgaagttgcggttttccttcaattatcacaaggttttgcgttaaattagcctgttcattgaacaggtatattcctgttactcctgaattttccggtgaaatcgctatttttaccgaagtttcaatagcctttccgttggctaatttatcaatatcgatgacatagcggtattcgctattcgtttgtatttcgccaagatattcatcttcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagaagatgaatatcttggcgaaatacaaacgaatagcgaataccgctatgtcatcgatattgataaattagccaacggaaaggctattgaaacttcggtaaaaatagcgatttcaccggaaaattcaggagtaacaggaatatacctgttcaatgaacaggctaatttaacgcaaaaccttgtgataattgaaggaaaaccgcaacttcagggtgacatttatatagaccttcaatggggaatctatggaaatatgagttattcgccgaaattatttaaaaaacgatattcactcaaagtaaaataaataaaagccggtttaattgccggctttatttttataatta Mask Tandem Repeat Region ================================================ taattataaaaataaagccggcaattaaaccggcttttatttattttactttgagtgaatatcgttttttaaataatttcggcgaataactcatatttccatagattccccattgaaggtctatataaatgtcaccctgaagttgcggttttccttcaattatcacaaggttttgcgttaaattagcctgttcattgaacaggtatattcctgttactcctgaattttccggtgaaatcgctatttttaccgaagtttcaatagcctttccgttggctaatttatcaatatcgatgacatagcggtattcgctattcgtttgtatttcgccaagatattcatcttcta Find is-nt database================================================ Query_seq: AA01499:AA01500|AA01499:AA01500:fructose 1,6-bisphosphatase protein:hypothetical protein:->->:1009508..1009855 348 taattataaaaataaagccggcaattaaaccggcttttatttattttactttgagtgaatatcgttttttaaataatttcggcgaataactcatatttccatagattccccattgaaggtctatataaatgtcaccctgaagttgcggttttccttcaattatcacaaggttttgcgttaaattagcctgttcattgaacaggtatattcctgttactcctgaattttccggtgaaatcgctatttttaccgaagtttcaatagcctttccgttggctaatttatcaatatcgatgacatagcggtattcgctattcgtttgtatttcgccaagatattcatcttcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01499:AA01500|AA01499:AA01500:fructose 1,6-bisphosphatase protein:hypothetical protein:->->:1009508..1009855 348 taattataaaaataaagccggcaattaaaccggcttttatttattttactttgagtgaatatcgttttttaaataatttcggcgaataactcatatttccatagattccccattgaaggtctatataaatgtcaccctgaagttgcggttttccttcaattatcacaaggttttgcgttaaattagcctgttcattgaacaggtatattcctgttactcctgaattttccggtgaaatcgctatttttaccgaagtttcaatagcctttccgttggctaatttatcaatatcgatgacatagcggtattcgctattcgtttgtatttcgccaagatattcatcttcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01499:AA01500|AA01499:AA01500:fructose 1,6-bisphosphatase protein:hypothetical protein:->->:1009508..1009855 348 taattataaaaataaagccggcaattaaaccggcttttatttattttactttgagtgaatatcgttttttaaataatttcggcgaataactcatatttccatagattccccattgaaggtctatataaatgtcaccctgaagttgcggttttccttcaattatcacaaggttttgcgttaaattagcctgttcattgaacaggtatattcctgttactcctgaattttccggtgaaatcgctatttttaccgaagtttcaatagcctttccgttggctaatttatcaatatcgatgacatagcggtattcgctattcgtttgtatttcgccaagatattcatcttcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taattataaaaataaagccggcaattaaaccggcttttatttattttactttgagtgaatatcgttttttaaataatttcggcgaataactcatatttccatagattccccattgaaggtctatataaatgtcaccctgaagttgcggttttccttcaattatcacaaggttttgcgttaaattagcctgttcattgaacaggtatattcctgttactcctgaattttccggtgaaatcgctatttttaccgaagtttcaatagcctttccgttggctaatttatcaatatcgatgacatagcggtattcgctattcgtttgtatttcgccaagatattcatcttcta Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 86 End: 178 ..................................................................................... M T H I S I D S P L K V Y I N V T L K L R F S F N Y H K V L R .......................................................................................................................................................................... Protein_Len: 37 Strand: + Start: 73 End: 183 ........................................................................ M I S A N N S Y F H R F P I E G L Y K C H P E V A V F L Q L S Q G F A L N ..................................................................................................................................................................... Protein_Len: 47 Strand: + Start: 159 End: 299 .............................................................................................................................................................. M I T R F C V K L A C S L N R Y I P V T P E F S G E I A I F T E V S I A F P L A N L S I S M T ................................................. Protein_Len: 60 Strand: - Start: 44 End: 325 ........................................... P K G E I I V L N Q T L N A Q E N F L Y I G T V G S N E P S I A I K V S T E I A K G N A L K D I D I V Y R Y E S N T Q M ....................... taattataaaaataaagccggcaattaaaccggcttttatttattttactttgagtgaatatcgttttttaaataatttcggcgaataactcatatttccatagattccccattgaaggtctatataaatgtcaccctgaagttgcggttttccttcaattatcacaaggttttgcgttaaattagcctgttcattgaacaggtatattcctgttactcctgaattttccggtgaaatcgctatttttaccgaagtttcaatagcctttccgttggctaatttatcaatatcgatgacatagcggtattcgctattcgtttgtatttcgccaagatattcatcttcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taattataaaaataaagccggcaattaaaccggcttttatttattttactttgagtgaatatcgttttttaaataatttcggcgaataactcatatttccatagattccccattgaaggtctatataaatgtcaccctgaagttgcggttttccttcaattatcacaaggttttgcgttaaattagcctgttcattgaacaggtatattcctgttactcctgaattttccggtgaaatcgctatttttaccgaagtttcaatagcctttccgttggctaatttatcaatatcgatgacatagcggtattcgctattcgtttgtatttcgccaagatattcatcttcta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 81 HP_score: -6.2 Tail_Score: -5.00267 Start: 17 End: 34 Full_Region: gtaaaataaataaaa gccggt ttaatt gccggc tttatttttataatt ................gccggtttaattgccggc.......................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 86 HP_score: -10.9 Tail_Score: -3.1917 Start: 187 End: 203 Full_Region: TTTGCGTTAAATTAG CCTGTTC ATT GAACAGG TATATTCCTGTTACT ..........................................................................................................................................................................................CCTGTTCATTGAACAGG................................................................................................................................................. Find igs database================================================ Query_seq: AA01499:AA01500|AA01499:AA01500:fructose 1,6-bisphosphatase protein:hypothetical protein:->->:1009508..1009855 348 taattataaaaataaagccggcaattaaaccggcttttatttattttactttgagtgaatatcgttttttaaataatttcggcgaataactcatatttccatagattccccattgaaggtctatataaatgtcaccctgaagttgcggttttccttcaattatcacaaggttttgcgttaaattagcctgttcattgaacaggtatattcctgttactcctgaattttccggtgaaatcgctatttttaccgaagtttcaatagcctttccgttggctaatttatcaatatcgatgacatagcggtattcgctattcgtttgtatttcgccaagatattcatcttcta Intra-Species Hit: Count: 1 Min: 1 Max: 348 Len: 348 Subject: aact_AA01499_AA01500|fructose 1,6-bisphosphatase protein:hypothetical protein|POSITIVE:POSITIVE|[1009508,1009855]|348 HSP 1 e-value: 0.0 bit: 690.0 Len: 348 Query Start:1 Query End:348 Subject Strand: POSITIVE Subject Start: 1 Subject End: 348 taattataaaaataaagccggcaattaaaccggcttttatttattttactttgagtgaatatcgttttttaaataatttcggcgaataactcatatttccatagattccccattgaaggtctatataaatgtcaccctgaagttgcggttttccttcaattatcacaaggttttgcgttaaattagcctgttcattgaacaggtatattcctgttactcctgaattttccggtgaaatcgctatttttaccgaagtttcaatagcctttccgttggctaatttatcaatatcgatgacatagcggtattcgctattcgtttgtatttcgccaagatattcatcttcta taattataaaaataaagccggcaattaaaccggcttttatttattttactttgagtgaatatcgttttttaaataatttcggcgaataactcatatttccatagattccccattgaaggtctatataaatgtcaccctgaagttgcggttttccttcaattatcacaaggttttgcgttaaattagcctgttcattgaacaggtatattcctgttactcctgaattttccggtgaaatcgctatttttaccgaagtttcaatagcctttccgttggctaatttatcaatatcgatgacatagcggtattcgctattcgtttgtatttcgccaagatattcatcttcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUAUAAAAAUAAAGCCGGCAAUUAAACCGGCUUUUAUUUAUUUUACUUUGAGUGAAUAUCGUUUUUUAAAUAAUUUCGGCGAAUAACUCAUAUUUCCAUAGAUUCCCCAUUGAAGGUCUAUAUAAAUGUCACCCUGAAGUUGCGGUUUUCCUUCAAUUAUCACAAGGUUUUGCGUUAAAUUAGCCUGUUCAUUGAACAGGUAUAUUCCUGUUACUCCUGAAUUUUCCGGUGAAAUCGCUAUUUUUACCGAAGUUUCAAUAGCCUUUCCGUUGGCUAAUUUAUCAAUAUCGAUGACAUAGCGGUAUUCGCUAUUCGUUUGUAUUUCGCCAAGAUAUUCAUCUUCUA .....(((((...((((((((........))))))))..)))))........(((((((((((...((......))....(((((...............(((((((..(((..(((((.((((...(((((.(((.((((((((.((....))..))))).)))...)))...)))))......((((((((...)))))))).................(((.((((.(((((((.......))))))))))).))).)))))))))....)))..))))))).(((((.(((((.((((((.....))))))))))).))))))))))..)))))))).)))... (-83.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAAGAUGAAUAUCUUGGCGAAAUACAAACGAAUAGCGAAUACCGCUAUGUCAUCGAUAUUGAUAAAUUAGCCAACGGAAAGGCUAUUGAAACUUCGGUAAAAAUAGCGAUUUCACCGGAAAAUUCAGGAGUAACAGGAAUAUACCUGUUCAAUGAACAGGCUAAUUUAACGCAAAACCUUGUGAUAAUUGAAGGAAAACCGCAACUUCAGGGUGACAUUUAUAUAGACCUUCAAUGGGGAAUCUAUGGAAAUAUGAGUUAUUCGCCGAAAUUAUUUAAAAAACGAUAUUCACUCAAAGUAAAAUAAAUAAAAGCCGGUUUAAUUGCCGGCUUUAUUUUUAUAAUUA .....((((((((((((((((((..((......((((((.....))))))((((((........((((((((((..((((((.((((((...((....))...))))))..)))..))).....((((...(.((((((......)))))))..))))..)))))))))).(((((....))))).....(((((...........))))).)))))).....(((((((((......)))..)))))).........))..))))))))...............)))))))).))........(((((..(((((((((......)))))))))...)))))..... (-94.86) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 307 320 274 193 146 aact:AA01108|5end_hypothetical_protein_763646..763876_POSITIVE 288 111 80 214 185 aact:AA00501|5end_hypothetical_protein_347293..347523_POSITIVE 288 111 80 72 43 aact:AA00500|5end_hypothetical_protein_347151..347381_POSITIVE 286 175 131 61 14 aact:AA01954|5end_excinuclease_ABC,_subunit_C_1320468..1320698_POSITIVE 281 330 289 230 189 aact:AA01965|5end_poly(A)_polymerase_1329478..1329708_POSITIVE 280 277 231 61 20 aact:AA02405|5end_conserved_hypothetical_protein_1652430..1652660_POSITIVE 280 334 291 70 15 aact:AA00163|5end_type_IV_prepilin_peptidase;_type_II_secretion_pathway_related_protein_116006..116236_POSITIVE 279 35 8 102 77 aact:AA00960|5end_possible_outer_membrane_protein_665265..665495_POSITIVE 278 275 236 131 80 aact:AA01434|5end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_961631..961861_POSITIVE 278 176 134 117 69 aact:AA01079|5end_conserved_hypothetical_protein_747404..747634_POSITIVE 277 226 182 68 21 aact:AA02196|5end_oxaloacetate_decarboxylase_beta_chain_1496308..1496538_POSITIVE 276 60 12 179 142 aact:AA01305|5end_conserved_hypothetical_protein_885598..885828_POSITIVE 273 240 191 44 2 aact:AA02139|5end_possible_membrane_protein_1461161..1461391_POSITIVE 273 84 49 165 129 aact:AA00452|5end_phosphomannomutase;_phosphoglucomutase_315288..315518_POSITIVE 271 333 302 231 200 aact:AA01357|5end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919133..919363_POSITIVE 270 244 201 193 163 aact:AA00492|5end_conserved_hypothetical_protein_343578..343808_POSITIVE 269 267 227 181 126 aact:AA02006|5end_PEP--fructosephosphotransferase_system_repressor,_DeoR_family_1363930..1364160_POSITIVE 267 320 271 203 154 aact:AA01757|5end_cobalt_transport_ATP-binding_protein_1187137..1187367_POSITIVE 267 312 271 170 130 aact:AA01114|5end_alcohol_dehydrogenase,_class_III;_glutathione-dependent_formaldehyde_dehydrogenase_765307..765537_POSITIVE 265 149 102 49 7 aact:AA02738|5end_phosphate_permease_1919372..1919602_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 333 279 236 149 108 aact:AA00096|3end_L-asparaginase_II_precursor_72406..72556_POSITIVE 298 150 111 144 103 aact:AA00764|3end_hemoglobin_binding_protein_A_527347..527497_POSITIVE 288 111 80 120 91 aact:AA00499|3end_pyridoxamine-5'-phosphate_oxidase_347279..347429_POSITIVE 282 330 292 43 7 aact:AA01291|3end_excinuclease_ABC_subunit_A_876001..876151_POSITIVE 281 330 289 110 69 aact:AA01963|3end_dnaK_suppressor_protein_1329438..1329588_POSITIVE 280 334 291 70 15 aact:AA00162|3end_pilin_C_biogenesis_protein/protein_transport_protein_116086..116236_POSITIVE 279 35 8 59 34 aact:AA00959|3end_nitrate/nitrite_response_regulator_665302..665452_POSITIVE 278 176 134 86 38 aact:AA01078|3end_transcriptional_regulator_(probable_MerR_family)_747453..747603_POSITIVE 277 226 182 54 7 aact:AA02195|3end_oxaloacetate_decarboxylase_alpha_chain_1496374..1496524_POSITIVE 276 60 12 81 44 aact:AA01304|3end_penicillin-binding_protein_1A_885580..885730_POSITIVE 273 84 49 145 109 aact:AA00451|3end_hypothetical_protein_315348..315498_POSITIVE 268 280 237 71 22 aact:AA01666|3end_penicillin-binding_protein_4;_D-alanyl-D-alanine_carboxypeptidase;_D-alanyl-D-alanine-endopeptidase_1129621..1129771_POSITIVE 265 340 298 138 84 aact:AA02676|3end_formate_dehydrogenase_alpha_subunit_1864177..1864327_POSITIVE 262 329 289 80 41 aact:AA00316|3end_conserved_hypothetical_protein_215340..215490_POSITIVE 260 277 231 136 81 aact:AA01561|3end_conserved_hypothetical_protein_1046971..1047121_POSITIVE 257 238 195 116 63 aact:AA01018|3end_conserved_hypothetical_protein_(possible_transcriptional_regulator)_712300..712450_POSITIVE 256 297 264 134 109 aact:AA02864|3end_glycerol-3-phosphate_transport_protein_2010192..2010342_POSITIVE 255 331 303 39 7 aact:AA01962|3end_recombination_protein_rec2_1328706..1328856_POSITIVE 254 278 231 122 68 aact:AA00592|3end_conserved_hypothetical_protein_(membrane/transport_protein)_407286..407436_POSITIVE 253 277 235 149 106 aact:AA00785|3end_conserved_hypothetical_protein_(possible_integrase)_539672..539822_POSITIVE