Origin IGS: aagtaatttcctctaaaacaaatggatgtaaagtagccatattttaccttacttaatcgtcttttttaaagcaaagaatcgcattaattcttttaattaaaattttctcttaaattttagtatgcaatcgtttgcttagtgctaaaatggacctatttcacatttttattttaagggatacatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aatgtatcccttaaaataaaaatgtgaaataggtccattttagcactaagcaaacgattgcatactaaaatttaagagaaaattttaattaaaagaattaatgcgattctttgctttaaaaaagacgattaagtaaggtaaaatatggctactttacatccatttgttttagaggaaattactt Mask Tandem Repeat Region ================================================ aagtaatttcctctaaaacaaatggatgtaaagtagccatattttaccttacttaatcgtcttttttaaagcaaagaatcgcattaattcttttaattaaaattttctcttaaattttagtatgcaatcgtttgcttagtgctaaaatggacctatttcacatttttattttaagggatacatt Find is-nt database================================================ Query_seq: AA01498:AA01499|AA01498:AA01499:conserved hypothetical protein:fructose 1,6-bisphosphatase protein:->->:1008313..1008496 184 aagtaatttcctctaaaacaaatggatgtaaagtagccatattttaccttacttaatcgtcttttttaaagcaaagaatcgcattaattcttttaattaaaattttctcttaaattttagtatgcaatcgtttgcttagtgctaaaatggacctatttcacatttttattttaagggatacatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01498:AA01499|AA01498:AA01499:conserved hypothetical protein:fructose 1,6-bisphosphatase protein:->->:1008313..1008496 184 aagtaatttcctctaaaacaaatggatgtaaagtagccatattttaccttacttaatcgtcttttttaaagcaaagaatcgcattaattcttttaattaaaattttctcttaaattttagtatgcaatcgtttgcttagtgctaaaatggacctatttcacatttttattttaagggatacatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01498:AA01499|AA01498:AA01499:conserved hypothetical protein:fructose 1,6-bisphosphatase protein:->->:1008313..1008496 184 aagtaatttcctctaaaacaaatggatgtaaagtagccatattttaccttacttaatcgtcttttttaaagcaaagaatcgcattaattcttttaattaaaattttctcttaaattttagtatgcaatcgtttgcttagtgctaaaatggacctatttcacatttttattttaagggatacatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aagtaatttcctctaaaacaaatggatgtaaagtagccatattttaccttacttaatcgtcttttttaaagcaaagaatcgcattaattcttttaattaaaattttctcttaaattttagtatgcaatcgtttgcttagtgctaaaatggacctatttcacatttttattttaagggatacatt Predict ORF larger than 30AA ================================================ aagtaatttcctctaaaacaaatggatgtaaagtagccatattttaccttacttaatcgtcttttttaaagcaaagaatcgcattaattcttttaattaaaattttctcttaaattttagtatgcaatcgtttgcttagtgctaaaatggacctatttcacatttttattttaagggatacatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 41 End: 70 ........................................TTTAAAAAAGACGATTAAGTAAGGTAAAAT.................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aagtaatttcctctaaaacaaatggatgtaaagtagccatattttaccttacttaatcgtcttttttaaagcaaagaatcgcattaattcttttaattaaaattttctcttaaattttagtatgcaatcgtttgcttagtgctaaaatggacctatttcacatttttattttaagggatacatt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 39 HP_score: -2.4 Tail_Score: -2.63584 Start: 84 End: 100 Full_Region: AAGCAAAGAATCGCA TTAATT CTTTT AATTAA AATTTTCTCTTAAAT ...................................................................................TTAATTCTTTTAATTAA.................................................................................... Find igs database================================================ Query_seq: AA01498:AA01499|AA01498:AA01499:conserved hypothetical protein:fructose 1,6-bisphosphatase protein:->->:1008313..1008496 184 aagtaatttcctctaaaacaaatggatgtaaagtagccatattttaccttacttaatcgtcttttttaaagcaaagaatcgcattaattcttttaattaaaattttctcttaaattttagtatgcaatcgtttgcttagtgctaaaatggacctatttcacatttttattttaagggatacatt Intra-Species Hit: Count: 1 Min: 1 Max: 184 Len: 184 Subject: aact_AA01498_AA01499|conserved hypothetical protein:fructose 1,6-bisphosphatase protein|POSITIVE:POSITIVE|[1008313,1008496]|184 HSP 1 e-value: 1.0E-100 bit: 365.0 Len: 184 Query Start:1 Query End:184 Subject Strand: POSITIVE Subject Start: 1 Subject End: 184 aagtaatttcctctaaaacaaatggatgtaaagtagccatattttaccttacttaatcgtcttttttaaagcaaagaatcgcattaattcttttaattaaaattttctcttaaattttagtatgcaatcgtttgcttagtgctaaaatggacctatttcacatttttattttaagggatacatt aagtaatttcctctaaaacaaatggatgtaaagtagccatattttaccttacttaatcgtcttttttaaagcaaagaatcgcattaattcttttaattaaaattttctcttaaattttagtatgcaatcgtttgcttagtgctaaaatggacctatttcacatttttattttaagggatacatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGUAAUUUCCUCUAAAACAAAUGGAUGUAAAGUAGCCAUAUUUUACCUUACUUAAUCGUCUUUUUUAAAGCAAAGAAUCGCAUUAAUUCUUUUAAUUAAAAUUUUCUCUUAAAUUUUAGUAUGCAAUCGUUUGCUUAGUGCUAAAAUGGACCUAUUUCACAUUUUUAUUUUAAGGGAUACAUU ..(((...(((..(((((.....((((((.((((((((((...................(((((........)))))...((((((.........((((((((((......))))))))))..((((....)))).))))))....))))..)))))).))))))...)))))..))))))... (-34.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUGUAUCCCUUAAAAUAAAAAUGUGAAAUAGGUCCAUUUUAGCACUAAGCAAACGAUUGCAUACUAAAAUUUAAGAGAAAAUUUUAAUUAAAAGAAUUAAUGCGAUUCUUUGCUUUAAAAAAGACGAUUAAGUAAGGUAAAAUAUGGCUACUUUACAUCCAUUUGUUUUAGAGGAAAUUACUU ......(((.((((((((((.(((((((.(((.(..((((((.(...(((((((.((((((((..((((((((......)))))))).((....))....)))))))).)))))))........((......))..).))))))..).))).)))))))...)))))))))).)))........ (-36.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 293 170 128 174 125 aact:AA00592|5end_conserved_hypothetical_protein_(membrane/transport_protein)_405536..405766_POSITIVE 250 159 117 137 86 aact:AA00368|5end_50S_ribosomal_protein_L4_247356..247586_POSITIVE 244 164 123 213 179 aact:AA00637|5end_phosphohistidine_phosphatase_438169..438399_POSITIVE 233 61 21 112 79 aact:AA01117|5end_signal_recognition_particle_protein_54_(GTP-binding_export_factor)_767919..768149_POSITIVE 223 180 132 141 94 aact:AA01170|5end_hypothetical_protein_802464..802694_POSITIVE 221 143 106 141 107 aact:AA02570|5end_conserved_hypothetical_protein_1789150..1789380_POSITIVE 221 166 124 202 161 aact:AA00463|5end_class_B_acid_phosphatase_323114..323344_POSITIVE 220 90 46 152 120 aact:AA00576|5end_conserved_hypothetical_protein_(possible_translation_factor)_394020..394250_POSITIVE 219 153 124 34 1 aact:AA01557|5end_recombinase_A_1044911..1045141_POSITIVE 213 97 55 100 56 aact:AA00424|5end_hypothetical_protein_287123..287353_POSITIVE 213 162 124 172 128 aact:AA00040|5end_thiol:disulfide_interchange_protein_31358..31588_POSITIVE 212 165 128 84 41 aact:AA01525|5end_amino_acid_ABC_transporter,_permease_component_1024277..1024507_POSITIVE 212 146 120 180 157 aact:AA00521|5end_phosphatase_357744..357974_POSITIVE 212 144 103 148 97 aact:AA00108|5end_conserved_hypothetical_protein_77957..78187_POSITIVE 208 178 135 222 171 aact:AA01114|5end_alcohol_dehydrogenase,_class_III;_glutathione-dependent_formaldehyde_dehydrogenase_765307..765537_POSITIVE 207 160 118 174 134 aact:AA00077|5end_conserved_hypothetical_protein_(possible_periplasmic_binding_transport_protein)_56111..56341_POSITIVE 204 94 56 56 24 aact:AA01009|5end_universal_stress_protein_A_704443..704673_POSITIVE 202 52 22 105 74 aact:AA01229|5end_50S_ribosomal_protein_L24_839222..839452_POSITIVE 202 179 131 203 159 aact:AA01063|5end_conserved_hypothetical_protein_736537..736767_POSITIVE 201 160 119 167 123 aact:AA01313|5end_deoxyuridinetriphosphatase;_deoxyuridine_5'-triphosphate_nucleotidohydrolase_889567..889797_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 293 170 128 65 16 aact:AA00591|3end_ABC_transporter,_ATP-binding_protein_405507..405657_POSITIVE 250 159 117 137 86 aact:AA00367|3end_50S_ribosomal_protein_L23_247436..247586_POSITIVE 221 143 106 95 61 aact:AA02569|3end_hypothetical_protein_1789184..1789334_POSITIVE 220 90 46 145 113 aact:AA00575|3end_shikimate_5-dehydrogenase_394093..394243_POSITIVE 219 99 59 90 49 aact:AA01983|3end_maltose_regulon_positive_regulatory_protein_1344738..1344888_POSITIVE 218 63 21 134 90 aact:AA01547|3end_deoxyribose-phosphate_aldolase_1037019..1037169_POSITIVE 216 54 20 122 82 aact:AA01263|3end_hypothetical_protein_861086..861236_POSITIVE 213 97 55 121 77 aact:AA00423|3end_GTP-binding_protein_287224..287374_POSITIVE 212 165 128 71 28 aact:AA01524|3end_amino_acid_ABC_transporter,_ATP-binding_protein_1024344..1024494_POSITIVE 212 144 103 134 83 aact:AA00107|3end_histidyl-tRNA_synthetase_78023..78173_POSITIVE 210 169 123 56 4 aact:AA00507|3end_hypothetical_protein_353201..353351_POSITIVE 208 178 135 84 33 aact:AA01113|3end_transcriptional_regulator_(merR_family)_765249..765399_POSITIVE 208 153 119 76 38 aact:AA00882|3end_hypothetical_protein_613645..613795_POSITIVE 207 160 118 66 26 aact:AA00076|3end_dihydroneopterin_aldolase_56083..56233_POSITIVE 205 180 131 88 37 aact:AA02333|3end_conserved_hypothetical_protein_1595184..1595334_POSITIVE 202 52 22 91 60 aact:AA01228|3end_50S_ribosomal_protein_L14_839288..839438_POSITIVE 202 179 131 85 41 aact:AA01062|3end_ribonuclease_PH_(RNase_PH)_736499..736649_POSITIVE 197 99 51 149 110 aact:AA01366|3end_16S_rRNA_processing_protein_923721..923871_POSITIVE 195 150 110 58 9 aact:AA01286|3end_5,10-methylenetetrahydrofolate_dehydrogenase_(bifunctional_protein)_871243..871393_POSITIVE 193 54 20 75 42 aact:AA01567|3end_conserved_hypothetical_protein_(possible_cell-wall-associated_hydrolase)_1055215..1055365_POSITIVE