Origin IGS: gattattttttatctcttttttgcattctgtaacggatcatctgatcctttaaaaaaattggctgctattctactgaaaatcccgtgtggataaaaggtcagtttgagaggaatagaagacgggatctgaagaaaggatccgacgttaataagtaataagatctttatctatatataaagtttattattactataattaagatcgacgaaaaccttggataagatcttttttatgttgtaaatgaaggagatggatcatgctacaagttgttaagatcttaccgatcctcctcaaatttcttgtgtataactctgttatttatccacagttggatcttttgtaaaatttattcgtgttataaaaacagatttttcacaattttatgtgaagttacccacaagaggttttagaaaatgactgattttattttgaacaaaaggatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatccttttgttcaaaataaaatcagtcattttctaaaacctcttgtgggtaacttcacataaaattgtgaaaaatctgtttttataacacgaataaattttacaaaagatccaactgtggataaataacagagttatacacaagaaatttgaggaggatcggtaagatcttaacaacttgtagcatgatccatctccttcatttacaacataaaaaagatcttatccaaggttttcgtcgatcttaattatagtaataataaactttatatatagataaagatcttattacttattaacgtcggatcctttcttcagatcccgtcttctattcctctcaaactgaccttttatccacacgggattttcagtagaatagcagccaatttttttaaaggatcagatgatccgttacagaatgcaaaaaagagataaaaaataatc Mask Tandem Repeat Region ================================================ gattattttttatctcttttttgcattctgtaacggatcatctgatcctttaaaaaaattggctgctattctactgaaaatcccgtgtggataaaaggtcagtttgagaggaatagaagacgggatctgaagaaaggatccgacgttaataagtaataagatctttatctatatataaagtttattattactataattaagatcgacgaaaaccttggataagatcttttttatgttgtaaatgaaggagatggatcatgctacaagttgttaagatcttaccgatcctcctcaaatttcttgtgtataactctgttatttatccacagttggatcttttgtaaaatttattcgtgttataaaaacagatttttcacaattttatgtgaagttacccacaagaggttttagaaaatgactgattttattttgaacaaaaggatta Find is-nt database================================================ Query_seq: AA01496:AA01497|AA01496:AA01497:glucose inhibited division protein A:MioC protein:->->:1007152..1007596 445 gattattttttatctcttttttgcattctgtaacggatcatctgatcctttaaaaaaattggctgctattctactgaaaatcccgtgtggataaaaggtcagtttgagaggaatagaagacgggatctgaagaaaggatccgacgttaataagtaataagatctttatctatatataaagtttattattactataattaagatcgacgaaaaccttggataagatcttttttatgttgtaaatgaaggagatggatcatgctacaagttgttaagatcttaccgatcctcctcaaatttcttgtgtataactctgttatttatccacagttggatcttttgtaaaatttattcgtgttataaaaacagatttttcacaattttatgtgaagttacccacaagaggttttagaaaatgactgattttattttgaacaaaaggatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01496:AA01497|AA01496:AA01497:glucose inhibited division protein A:MioC protein:->->:1007152..1007596 445 gattattttttatctcttttttgcattctgtaacggatcatctgatcctttaaaaaaattggctgctattctactgaaaatcccgtgtggataaaaggtcagtttgagaggaatagaagacgggatctgaagaaaggatccgacgttaataagtaataagatctttatctatatataaagtttattattactataattaagatcgacgaaaaccttggataagatcttttttatgttgtaaatgaaggagatggatcatgctacaagttgttaagatcttaccgatcctcctcaaatttcttgtgtataactctgttatttatccacagttggatcttttgtaaaatttattcgtgttataaaaacagatttttcacaattttatgtgaagttacccacaagaggttttagaaaatgactgattttattttgaacaaaaggatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01496:AA01497|AA01496:AA01497:glucose inhibited division protein A:MioC protein:->->:1007152..1007596 445 gattattttttatctcttttttgcattctgtaacggatcatctgatcctttaaaaaaattggctgctattctactgaaaatcccgtgtggataaaaggtcagtttgagaggaatagaagacgggatctgaagaaaggatccgacgttaataagtaataagatctttatctatatataaagtttattattactataattaagatcgacgaaaaccttggataagatcttttttatgttgtaaatgaaggagatggatcatgctacaagttgttaagatcttaccgatcctcctcaaatttcttgtgtataactctgttatttatccacagttggatcttttgtaaaatttattcgtgttataaaaacagatttttcacaattttatgtgaagttacccacaagaggttttagaaaatgactgattttattttgaacaaaaggatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gattattttttatctcttttttgcattctgtaacggatcatctgatcctttaaaaaaattggctgctattctactgaaaatcccgtgtggataaaaggtcagtttgagaggaatagaagacgggatctgaagaaaggatccgacgttaataagtaataagatctttatctatatataaagtttattattactataattaagatcgacgaaaaccttggataagatcttttttatgttgtaaatgaaggagatggatcatgctacaagttgttaagatcttaccgatcctcctcaaatttcttgtgtataactctgttatttatccacagttggatcttttgtaaaatttattcgtgttataaaaacagatttttcacaattttatgtgaagttacccacaagaggttttagaaaatgactgattttattttgaacaaaaggatta Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 2 End: 91 . M I F Y L F F A F C N G S S D P L K K L A A I L L K I P C G .................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 32 Strand: + Start: 58 End: 153 ......................................................... M G C Y S T E N P V W I K G Q F E R N R R R D L K K G S D V N K .................................................................................................................................................................................................................................................................................................... Protein_Len: 47 Strand: + Start: 268 End: 408 ........................................................................................................................................................................................................................................................................... M L R S Y R S S S N F L C I T L L F I H S W I F C K I Y S C Y K N R F F T I L C E V T H K R F ..................................... Protein_Len: 40 Strand: - Start: 200 End: 319 ....................................................................................................................................................................................................... S R R F G Q I L D K K H Q L H L L H I M S C T T L I K G I R R L N R T Y L E T M .............................................................................................................................. Protein_Len: 52 Strand: - Start: 13 End: 168 ............ R K K A N Q L P D D S G K F F N A A I R S F I G H P Y F T L K L P I S S P I Q L F S G V N I L L L I K M ..................................................................................................................................................................................................................................................................................... gattattttttatctcttttttgcattctgtaacggatcatctgatcctttaaaaaaattggctgctattctactgaaaatcccgtgtggataaaaggtcagtttgagaggaatagaagacgggatctgaagaaaggatccgacgttaataagtaataagatctttatctatatataaagtttattattactataattaagatcgacgaaaaccttggataagatcttttttatgttgtaaatgaaggagatggatcatgctacaagttgttaagatcttaccgatcctcctcaaatttcttgtgtataactctgttatttatccacagttggatcttttgtaaaatttattcgtgttataaaaacagatttttcacaattttatgtgaagttacccacaagaggttttagaaaatgactgattttattttgaacaaaaggatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gattattttttatctcttttttgcattctgtaacggatcatctgatcctttaaaaaaattggctgctattctactgaaaatcccgtgtggataaaaggtcagtttgagaggaatagaagacgggatctgaagaaaggatccgacgttaataagtaataagatctttatctatatataaagtttattattactataattaagatcgacgaaaaccttggataagatcttttttatgttgtaaatgaaggagatggatcatgctacaagttgttaagatcttaccgatcctcctcaaatttcttgtgtataactctgttatttatccacagttggatcttttgtaaaatttattcgtgttataaaaacagatttttcacaattttatgtgaagttacccacaagaggttttagaaaatgactgattttattttgaacaaaaggatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01496:AA01497|AA01496:AA01497:glucose inhibited division protein A:MioC protein:->->:1007152..1007596 445 gattattttttatctcttttttgcattctgtaacggatcatctgatcctttaaaaaaattggctgctattctactgaaaatcccgtgtggataaaaggtcagtttgagaggaatagaagacgggatctgaagaaaggatccgacgttaataagtaataagatctttatctatatataaagtttattattactataattaagatcgacgaaaaccttggataagatcttttttatgttgtaaatgaaggagatggatcatgctacaagttgttaagatcttaccgatcctcctcaaatttcttgtgtataactctgttatttatccacagttggatcttttgtaaaatttattcgtgttataaaaacagatttttcacaattttatgtgaagttacccacaagaggttttagaaaatgactgattttattttgaacaaaaggatta Intra-Species Hit: Count: 1 Min: 23 Max: 445 Len: 423 Subject: aact_AA01496_AA01497|glucose inhibited division protein A:MioC protein|POSITIVE:POSITIVE|[1007152,1007596]|445 HSP 1 e-value: 0.0 bit: 797.0 Len: 423 Query Start:23 Query End:445 Subject Strand: POSITIVE Subject Start: 23 Subject End: 445 ......................gcattctgtaacggatcatctgatcctttnnnnnnnttggctgctattctactgaaaatcccgtgtggataaaaggtcagtttgagaggaatagaagacgggatctgaagaaaggatccgacgttaataagtaataagatctttatctatatataaagtttattattactataattaagatcgacgaaaaccttggataagatcttttttatgttgtaaatgaaggagatggatcatgctacaagttgttaagatcttaccgatcctcctcaaatttcttgtgtataactctgttatttatccacagttggatcttttgtaaaatttattcgtgttataaaaacagatttttcacaattttatgtgaagttacccacaagaggttttagaaaatgactgattttattttgaacaaaaggatta ......................gcattctgtaacggatcatctgatcctttaaaaaaattggctgctattctactgaaaatcccgtgtggataaaaggtcagtttgagaggaatagaagacgggatctgaagaaaggatccgacgttaataagtaataagatctttatctatatataaagtttattattactataattaagatcgacgaaaaccttggataagatcttttttatgttgtaaatgaaggagatggatcatgctacaagttgttaagatcttaccgatcctcctcaaatttcttgtgtataactctgttatttatccacagttggatcttttgtaaaatttattcgtgttataaaaacagatttttcacaattttatgtgaagttacccacaagaggttttagaaaatgactgattttattttgaacaaaaggatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCAUUCUGUAACGGAUCAUCUGAUCCUUUAAAAAAAUUGGCUGCUAUUCUACUGAAAAUCCCGUGUGGAUAAAAGGUCAGUUUGAGAGGAAUAGAAGACGGGAUCUGAAGAAAGGAUCCGACGUUAAUAAGUAAUAAGAUCUUUAUCUAUAUAUAAAGUUUAUUAUUACUAUAAUUAAGAUCGACGAAAACCUUGGAUAAGAUCUUUUUUAUGUUGUAAAUGAAGGAGAUGGAUCAUGCUACAAGUUGUUAAGAUCUUACCGAUCCUCCUCAAAUUUCUUGUGUAUAACUCUGUUAUUUAUCCACAGUUGGAUCUUUUGUAAAAUUUAUUCGUGUUAUAAAAACAGAUUUUUCACAAUUUUAUGUGAAGUUACCCACAAGAGGUUUUAGAAAAUGACUGAUUUUAUUUUGAACAAAAGGAUUA ........(((((..((.((((.((((((....(((((((((.....(((((............))))).....))))))))).)))))).)))).))..((((((.......))))))..)))))...((((((((...((((((......))))))....)))))))).....((((((((((((........(((...(((((((((((.......)))))))))))..)))..........)))))..)))))))..((((((.((.((((((((((((..((((((((((....(((((....)))))..(((((((.((......)).)))))))))))))...((((((......))))))))))..)))))))))))).))(((((((......))))))).......)))))). (-103.37) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCCUUUUGUUCAAAAUAAAAUCAGUCAUUUUCUAAAACCUCUUGUGGGUAACUUCACAUAAAAUUGUGAAAAAUCUGUUUUUAUAACACGAAUAAAUUUUACAAAAGAUCCAACUGUGGAUAAAUAACAGAGUUAUACACAAGAAAUUUGAGGAGGAUCGGUAAGAUCUUAACAACUUGUAGCAUGAUCCAUCUCCUUCAUUUACAACAUAAAAAAGAUCUUAUCCAAGGUUUUCGUCGAUCUUAAUUAUAGUAAUAAUAAACUUUAUAUAUAGAUAAAGAUCUUAUUACUUAUUAACGUCGGAUCCUUUCUUCAGAUCCCGUCUUCUAUUCCUCUCAAACUGACCUUUUAUCCACACGGGAUUUUCAGUAGAAUAGCAGCCAAUUUUUUUAAAGGAUCAGAUGAUCCGUUACAGAAUGC ........((((((...((((((.(((............((((.....))))...((((((......))))))....))).)))))).....))))))(((((((..(((((((...((((((((((....((((..(((...((((.(((((((((((((((((((((((((......((((((.((((..........)))))))))).......)))))))))))............((((.(((((...((((((((....((((((......))))))...)))))))).))))).)))))))))..)))))))))....)))).)))..))))...........))))))))))..)))))))..)))))))..........((((.(((.(((((....))))).))).))))... (-97.88) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 297 70 21 119 67 aact:AA02976|5end_UDP-2,3-diacylglucosamine_hydrolase_2080229..2080459_POSITIVE 288 254 212 179 136 aact:AA02015|5end_phosphoheptose_isomerase_1371059..1371289_POSITIVE 260 110 61 212 153 aact:AA02889|5end_hypothetical_protein_2025045..2025275_POSITIVE 260 130 83 97 52 aact:AA02797|5end_carbohydrate_kinase;_myo-inositol_catabolism_protein_1963883..1964113_POSITIVE 259 126 91 79 43 aact:AA02390|5end_mannonate_dehydratase_1639303..1639533_POSITIVE 259 103 61 105 61 aact:AA01704|5end_tyrosine-specific_transport_protein_1156170..1156400_POSITIVE 259 100 52 103 60 aact:AA00362|5end_30S_ribosomal_protein_S3_244847..245077_POSITIVE 258 100 60 102 60 aact:AA02860|5end_conserved_hypothetical_protein_2006074..2006304_POSITIVE 258 98 60 107 66 aact:AA01487|5end_ATP_synthase_F1,_subunit_delta_1000114..1000344_POSITIVE 257 67 36 161 136 aact:AA00796|5end_iron(III)_dicitrate_transport_system_permease_protein_545686..545916_POSITIVE 256 105 61 168 131 aact:AA01375|5end_hypothetical_protein_931020..931250_POSITIVE 254 84 44 193 161 aact:AA02271|5end_hypothetical_protein_1548623..1548853_POSITIVE 254 270 236 161 129 aact:AA01255|5end_hypothetical_protein_852656..852886_POSITIVE 251 120 75 204 158 aact:AA00169|5end_conserved_hypothetical_protein_120312..120542_POSITIVE 250 256 212 131 87 aact:AA01179|5end_beta-ketoacyl-ACP_reductase;_short-chain_alcohol_dehydrogenase_807996..808226_POSITIVE 249 100 62 230 181 aact:AA01289|5end_hypothetical_protein_872385..872615_POSITIVE 248 117 73 71 19 aact:AA00357|5end_transposase_(probable_IS200-like_)_243334..243564_POSITIVE 247 76 37 227 182 aact:AA01107|5end_adenylosuccinate_lyase_762310..762540_POSITIVE 247 120 76 149 102 aact:AA00383|5end_chloride_channel_protein_258894..259124_POSITIVE 246 102 63 84 52 aact:AA02334|5end_outer_membrane_protein_P1_1595307..1595537_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 338 110 61 68 9 aact:AA01525|3end_amino_acid_ABC_transporter,_permease_component_1025079..1025229_POSITIVE 314 104 62 57 4 aact:AA01288|3end_hypothetical_protein_872288..872438_POSITIVE 297 70 21 123 71 aact:AA02975|3end_conserved_hypothetical_protein_2080313..2080463_POSITIVE 281 102 63 134 88 aact:AA01033|3end_Na(+)-translocating_NADH-quinone_reductase_subunit_A_723435..723585_POSITIVE 276 44 8 150 114 aact:AA01988|3end_dihydrolipoamide_dehydrogenase;_lipoamide_dehydrogenase_1352018..1352168_POSITIVE 260 110 61 148 89 aact:AA02888|3end_ssrA-binding_protein,_small_protein_B_2025061..2025211_POSITIVE 260 130 83 51 6 aact:AA02796|3end_possible_transcriptional_regulator,_RpiR_family_1963917..1964067_POSITIVE 259 126 91 56 20 aact:AA02389|3end_transcriptional_repressor_for_uxu_operon_1639360..1639510_POSITIVE 259 100 52 86 43 aact:AA00361|3end_50S_ribosomal_protein_L16_244910..245060_POSITIVE 258 98 60 91 50 aact:AA01486|3end_ATP_synthase_F1,_subunit_alpha_1000178..1000328_POSITIVE 254 270 236 127 95 aact:AA01254|3end_conserved_hypothetical_protein_(possible_proline_dipeptidase)_852702..852852_POSITIVE 251 120 75 52 6 aact:AA00168|3end_possible_sigma_54_modulation_protein_120240..120390_POSITIVE 250 84 53 151 113 aact:AA02564|3end_transketolase_N-terminal_section_1786783..1786933_POSITIVE 250 256 212 108 64 aact:AA01177|3end_beta-ketoacyl-ACP_synthase_IV_808053..808203_POSITIVE 248 103 62 51 3 aact:AA00653|3end_hypothetical_protein_447860..448010_POSITIVE 247 210 169 89 50 aact:AA02103|3end_D-alanyl-D-alanine-endopeptidase;_penicillin-binding_protein_7_1431784..1431934_POSITIVE 247 120 76 60 13 aact:AA00382|3end_hypothetical_protein_258885..259035_POSITIVE 245 262 221 131 92 aact:AA00175|3end_cell_filamentation_protein_124589..124739_POSITIVE 244 80 32 96 51 aact:AA01335|3end_primosomal_protein_N'_904109..904259_POSITIVE 242 68 21 145 96 aact:AA02270|3end_endopeptidase_La;_lon_protease;_protease_La_homolog;_ATP-dependent_protease_LA_(lon-1)_1548668..1548818_POSITIVE