Origin IGS: taacaaggtttgatgcttaaccgaggatattcgctatcctcggtttttaaccttaggctggcaaaaatgcgtgataggcttagccgatctcacctcatcgccccgcacatttacttcttataaggcgagataacccggaaatgtatatggtgcttaatgtatttaaagacatggatttgaggatttatcaggaattattgggttagtgataaaaaagtgcggtcaaaatcgatcgcacttttttgttttttagcgagagggaaagattactagatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatctagtaatctttccctctcgctaaaaaacaaaaaagtgcgatcgattttgaccgcacttttttatcactaacccaataattcctgataaatcctcaaatccatgtctttaaatacattaagcaccatatacatttccgggttatctcgccttataagaagtaaatgtgcggggcgatgaggtgagatcggctaagcctatcacgcatttttgccagcctaaggttaaaaaccgaggatagcgaatatcctcggttaagcatcaaaccttgtta Mask Tandem Repeat Region ================================================ taacaaggtttgatgcttaaccgaggatattcgctatcctcggtttttaaccttaggctggcaaaaatgcgtgataggcttagccgatctcacctcatcgccccgcacatttacttcttataaggcgagataacccggaaatgtatatggtgcttaatgtatttaaagacatggatttgaggatttatcaggaattattgggttagtgataaaaaagtgcggtcaaaatcgatcgcacttttttgttttttagcgagagggaaagattactagatta Find is-nt database================================================ Query_seq: AA01495:AA01496|AA01495:AA01496:probable oxidoreductase:glucose inhibited division protein A:->->:1004988..1005264 277 taacaaggtttgatgcttaaccgaggatattcgctatcctcggtttttaaccttaggctggcaaaaatgcgtgataggcttagccgatctcacctcatcgccccgcacatttacttcttataaggcgagataacccggaaatgtatatggtgcttaatgtatttaaagacatggatttgaggatttatcaggaattattgggttagtgataaaaaagtgcggtcaaaatcgatcgcacttttttgttttttagcgagagggaaagattactagatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01495:AA01496|AA01495:AA01496:probable oxidoreductase:glucose inhibited division protein A:->->:1004988..1005264 277 taacaaggtttgatgcttaaccgaggatattcgctatcctcggtttttaaccttaggctggcaaaaatgcgtgataggcttagccgatctcacctcatcgccccgcacatttacttcttataaggcgagataacccggaaatgtatatggtgcttaatgtatttaaagacatggatttgaggatttatcaggaattattgggttagtgataaaaaagtgcggtcaaaatcgatcgcacttttttgttttttagcgagagggaaagattactagatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01495:AA01496|AA01495:AA01496:probable oxidoreductase:glucose inhibited division protein A:->->:1004988..1005264 277 taacaaggtttgatgcttaaccgaggatattcgctatcctcggtttttaaccttaggctggcaaaaatgcgtgataggcttagccgatctcacctcatcgccccgcacatttacttcttataaggcgagataacccggaaatgtatatggtgcttaatgtatttaaagacatggatttgaggatttatcaggaattattgggttagtgataaaaaagtgcggtcaaaatcgatcgcacttttttgttttttagcgagagggaaagattactagatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacaaggtttgatgcttaaccgaggatattcgctatcctcggtttttaaccttaggctggcaaaaatgcgtgataggcttagccgatctcacctcatcgccccgcacatttacttcttataaggcgagataacccggaaatgtatatggtgcttaatgtatttaaagacatggatttgaggatttatcaggaattattgggttagtgataaaaaagtgcggtcaaaatcgatcgcacttttttgttttttagcgagagggaaagattactagatta Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 10 End: 120 ......... M M L N R G Y S L S S V F N L R L A K M R D R L S R S H L I A P H I Y F L ............................................................................................................................................................. Protein_Len: 34 Strand: + Start: 29 End: 130 ............................ M R Y P R F L T L G W Q K C V I G L A D L T S S P R T F T S Y K A R ................................................................................................................................................... Protein_Len: 39 Strand: + Start: 87 End: 203 ...................................................................................... M S P H R P A H L L L I R R D N P E M Y M V L N V F K D M D L R I Y Q E L L G .......................................................................... taacaaggtttgatgcttaaccgaggatattcgctatcctcggtttttaaccttaggctggcaaaaatgcgtgataggcttagccgatctcacctcatcgccccgcacatttacttcttataaggcgagataacccggaaatgtatatggtgcttaatgtatttaaagacatggatttgaggatttatcaggaattattgggttagtgataaaaaagtgcggtcaaaatcgatcgcacttttttgttttttagcgagagggaaagattactagatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacaaggtttgatgcttaaccgaggatattcgctatcctcggtttttaaccttaggctggcaaaaatgcgtgataggcttagccgatctcacctcatcgccccgcacatttacttcttataaggcgagataacccggaaatgtatatggtgcttaatgtatttaaagacatggatttgaggatttatcaggaattattgggttagtgataaaaaagtgcggtcaaaatcgatcgcacttttttgttttttagcgagagggaaagattactagatta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 47 HP_score: -4.3 Tail_Score: -2.53263 Start: 25 End: 39 Full_Region: aaggttaaaaaccga ggata gcgaa tatcc tcggttaagcatcaa ........................ggatagcgaatatcc.............................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 100 HP_score: -8.0 Tail_Score: -6.04324 Start: 217 End: 238 Full_Region: GTTAGTGATAAAAAA GTGCGGTC AAAATC GATCGCAC TTTTTTGTTTTTTAG ........................................................................................................................................................................................................................GTGCGGTCAAAATCGATCGCAC....................................... TransTerm Strand: - Conf: 88 HP_score: -6.5 Tail_Score: -5.28176 Start: 217 End: 238 Full_Region: ctaaaaaacaaaaaa gtgcgatcg attt tgaccgcac ttttttatcactaac ........................................................................................................................................................................................................................gtgcgatcgattttgaccgcac....................................... Find igs database================================================ Query_seq: AA01495:AA01496|AA01495:AA01496:probable oxidoreductase:glucose inhibited division protein A:->->:1004988..1005264 277 taacaaggtttgatgcttaaccgaggatattcgctatcctcggtttttaaccttaggctggcaaaaatgcgtgataggcttagccgatctcacctcatcgccccgcacatttacttcttataaggcgagataacccggaaatgtatatggtgcttaatgtatttaaagacatggatttgaggatttatcaggaattattgggttagtgataaaaaagtgcggtcaaaatcgatcgcacttttttgttttttagcgagagggaaagattactagatta Intra-Species Hit: Count: 1 Min: 1 Max: 238 Len: 238 Subject: aact_AA01495_AA01496|probable oxidoreductase:glucose inhibited division protein A|POSITIVE:POSITIVE|[1004988,1005264]|277 HSP 1 e-value: 1.0E-132 bit: 472.0 Len: 238 Query Start:1 Query End:238 Subject Strand: POSITIVE Subject Start: 1 Subject End: 238 taacaaggtttgatgcttaaccgaggatattcgctatcctcggtttttaaccttaggctggcaaaaatgcgtgataggcttagccgatctcacctcatcgccccgcacatttacttcttataaggcgagataacccggaaatgtatatggtgcttaatgtatttaaagacatggatttgaggatttatcaggaattattgggttagtgataaaaaagtgcggtcaaaatcgatcgcac....................................... taacaaggtttgatgcttaaccgaggatattcgctatcctcggtttttaaccttaggctggcaaaaatgcgtgataggcttagccgatctcacctcatcgccccgcacatttacttcttataaggcgagataacccggaaatgtatatggtgcttaatgtatttaaagacatggatttgaggatttatcaggaattattgggttagtgataaaaaagtgcggtcaaaatcgatcgcac....................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACAAGGUUUGAUGCUUAACCGAGGAUAUUCGCUAUCCUCGGUUUUUAACCUUAGGCUGGCAAAAAUGCGUGAUAGGCUUAGCCGAUCUCACCUCAUCGCCCCGCACAUUUACUUCUUAUAAGGCGAGAUAACCCGGAAAUGUAUAUGGUGCUUAAUGUAUUUAAAGACAUGGAUUUGAGGAUUUAUCAGGAAUUAUUGGGUUAGUGAUAAAAAAGUGCGGUCAAAAUCGAUCGCAC ....((((((........(((((((((((.....)))))))))))...)))))).(((((((...............)).)))))....((((....(((((.....................)))))..((((((((..((((....(((((.....))))).....))))...(((((.......))))).....)))))))))))).......((((((((......)))))))) (-64.46) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUGCGAUCGAUUUUGACCGCACUUUUUUAUCACUAACCCAAUAAUUCCUGAUAAAUCCUCAAAUCCAUGUCUUUAAAUACAUUAAGCACCAUAUACAUUUCCGGGUUAUCUCGCCUUAUAAGAAGUAAAUGUGCGGGGCGAUGAGGUGAGAUCGGCUAAGCCUAUCACGCAUUUUUGCCAGCCUAAGGUUAAAAACCGAGGAUAGCGAAUAUCCUCGGUUAAGCAUCAAACCUUGUUA (((((.(((....))).)))))...(((((((..((........))..)))))))..............................((((.((.(((.(((..((((......))))....))).))).))))))..(((((....(((.(((.(((...))).))).)))....)))))....(((((((...(((((((((((.....)))))))))))........)))))))... (-58.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 376 80 38 192 153 aact:AA01146|5end_conserved_hypothetical_protein_784433..784663_POSITIVE 367 106 67 64 29 aact:AA00603|5end_undecaprenyl_pyrophosphate_synthetase_(UPPS)_410283..410513_POSITIVE 364 108 69 155 109 aact:AA02427|5end_hypothetical_protein_1674185..1674415_POSITIVE 362 129 83 191 152 aact:AA00820|5end_succinyl-diaminopimelate_desuccinylase_563326..563556_POSITIVE 354 126 82 222 180 aact:AA02545|5end_conserved_hypothetical_protein_1773965..1774195_POSITIVE 352 224 190 123 87 aact:AA02700|5end_succinyl-CoA_synthase,_alpha_subunit_1883256..1883486_POSITIVE 348 225 185 90 48 aact:AA02335|5end_6-O-methylguanine-DNA_methyltransferase_1596814..1597044_POSITIVE 343 106 67 59 9 aact:AA00684|5end_adenylosuccinate_synthase_467486..467716_POSITIVE 341 87 41 133 94 aact:AA01274|5end_hypothetical_protein_864519..864749_POSITIVE 338 106 65 210 170 aact:AA00333|5end_1-phosphofructokinase;_tagatose-6-phosphate_kinase_225543..225773_POSITIVE 337 128 85 191 143 aact:AA02409|5end_DNA_ligase_1653315..1653545_POSITIVE 329 108 69 165 123 aact:AA00582|5end_conserved_hypothetical_protein_397904..398134_POSITIVE 324 43 2 83 33 aact:AA01066|5end_conserved_hypothetical_protein_738380..738610_POSITIVE 322 230 199 181 149 aact:AA02754|5end_conserved_hypothetical_protein_1928949..1929179_POSITIVE 322 110 61 84 24 aact:AA00164|5end_dephospho-CoA_kinase_(dephosphocoenzyme_A_kinase)_116745..116975_POSITIVE 317 106 70 61 18 aact:AA02231|5end_probable_dethiobiotin_synthetase_1_1517650..1517880_POSITIVE 317 105 65 172 130 aact:AA01531|5end_hypothetical_protein_1028705..1028935_POSITIVE 313 100 52 187 137 aact:AA01522|5end_conserved_hypothetical_protein_1022840..1023070_POSITIVE 313 230 189 177 142 aact:AA00337|5end_conserved_hypothetical_protein_228264..228494_POSITIVE 312 90 41 136 82 aact:AA02044|5end_phosphotransferase_system_enzyme_II,_galactitol_specific,_protein_A_1391488..1391718_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 376 80 38 139 100 aact:AA01145|3end_glycyl-tRNA_synthetase_alpha_subunit_784460..784610_POSITIVE 364 108 69 116 70 aact:AA02426|3end_uridylyltransferase_1674226..1674376_POSITIVE 354 126 82 122 80 aact:AA02544|3end_ATP-dependent_RNA_helicase_1773945..1774095_POSITIVE 347 70 21 103 48 aact:AA01495|3end_probable_oxidoreductase_1004927..1005077_POSITIVE 341 87 41 122 83 aact:AA01273|3end_hypothetical_protein_864588..864738_POSITIVE 329 108 69 147 105 aact:AA00581|3end_thiosulfate_sulfurtransferase_(rhodanese)_397966..398116_POSITIVE 327 109 76 139 106 aact:AA02874|3end_hypothetical_protein_2017899..2018049_POSITIVE 320 47 7 120 85 aact:AA00370|3end_30S_ribosomal_protein_S10_249008..249158_POSITIVE 317 105 65 136 94 aact:AA01530|3end_conserved_hypothetical_protein_1028749..1028899_POSITIVE 313 230 189 63 28 aact:AA00335|3end_PTS_fructose-specific_IIBC_component_228230..228380_POSITIVE 311 107 64 151 102 aact:AA00993|3end_possible_D-tyrosyl-tRNA(Tyr)_deacylase_694999..695149_POSITIVE 309 149 101 93 47 aact:AA01526|3end_amino_acid_ABC_transporter,_periplasmic_amino_acid-binding_protein_1025839..1025989_POSITIVE 307 88 41 112 57 aact:AA01589|3end_conserved_hypothetical_protein_1070871..1071021_POSITIVE 305 128 84 146 87 aact:AA01690|3end_penicillin-binding_protein_1C_1149142..1149292_POSITIVE 305 87 41 67 12 aact:AA01232|3end_30S_ribosomal_protein_S8_840914..841064_POSITIVE 301 116 79 57 17 aact:AA00992|3end_ribonuclease_BN_694568..694718_POSITIVE 299 117 71 94 54 aact:AA02420|3end_ISRSO11_related_transposase_1662668..1662818_POSITIVE 299 105 62 134 89 aact:AA01995|3end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355394..1355544_POSITIVE 297 108 61 146 106 aact:AA00408|3end_SUN_protein_(FMU_protein)_273688..273838_POSITIVE 296 90 42 147 93 aact:AA01520|3end_hypothetical_protein_1022887..1023037_POSITIVE