Origin IGS: ttttatttccttaaaaatcaatttattaaaaataaatttaaatttattccaaaacttacgtttatgtaagtacttctcaaaagtaagtaatgtaattatagttgctattgaataatacggtcaaccatcccaataccaggaggaaaacaacataataaacaaaactatcgccattacaggtgcaacaggtcaattcggtgctatcgcattagatttattaaaagcaaaaagcgtcaacgttatcgccctt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aagggcgataacgttgacgctttttgcttttaataaatctaatgcgatagcaccgaattgacctgttgcacctgtaatggcgatagttttgtttattatgttgttttcctcctggtattgggatggttgaccgtattattcaatagcaactataattacattacttacttttgagaagtacttacataaacgtaagttttggaataaatttaaatttatttttaataaattgatttttaaggaaataaaa Mask Tandem Repeat Region ================================================ ttttatttccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaatttattccaaaacttacgtttatgtaagtacttctcaaaagtaagtaatgtaattatagttgctattgaataatacggtcaaccatcccaataccaggaggaaaacaacataataaacaaaactatcgccattacaggtgcaacaggtcaattcggtgctatcgcattagatttattaaaagcaaaaagcgtcaacgttatcgccctt Find is-nt database================================================ Query_seq: AA01494:AA01495|AA01494:AA01495:conserved hypothetical protein:probable oxidoreductase:->->:1003982..1004231 250 ttttatttccttaaaaatcaatttattaaaaataaatttaaatttattccaaaacttacgtttatgtaagtacttctcaaaagtaagtaatgtaattatagttgctattgaataatacggtcaaccatcccaataccaggaggaaaacaacataataaacaaaactatcgccattacaggtgcaacaggtcaattcggtgctatcgcattagatttattaaaagcaaaaagcgtcaacgttatcgccctt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01494:AA01495|AA01494:AA01495:conserved hypothetical protein:probable oxidoreductase:->->:1003982..1004231 250 ttttatttccttaaaaatcaatttattaaaaataaatttaaatttattccaaaacttacgtttatgtaagtacttctcaaaagtaagtaatgtaattatagttgctattgaataatacggtcaaccatcccaataccaggaggaaaacaacataataaacaaaactatcgccattacaggtgcaacaggtcaattcggtgctatcgcattagatttattaaaagcaaaaagcgtcaacgttatcgccctt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01494:AA01495|AA01494:AA01495:conserved hypothetical protein:probable oxidoreductase:->->:1003982..1004231 250 ttttatttccttaaaaatcaatttattaaaaataaatttaaatttattccaaaacttacgtttatgtaagtacttctcaaaagtaagtaatgtaattatagttgctattgaataatacggtcaaccatcccaataccaggaggaaaacaacataataaacaaaactatcgccattacaggtgcaacaggtcaattcggtgctatcgcattagatttattaaaagcaaaaagcgtcaacgttatcgccctt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttttatttccttaaaaatcaatttattaaaaataaatttaaatttattccaaaacttacgtttatgtaagtacttctcaaaagtaagtaatgtaattatagttgctattgaataatacggtcaaccatcccaataccaggaggaaaacaacataataaacaaaactatcgccattacaggtgcaacaggtcaattcggtgctatcgcattagatttattaaaagcaaaaagcgtcaacgttatcgccctt Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 5 End: 112 .... M S L K I N L L K I N L N L F Q N L R L C K Y F S K V S N V I I V A I E .......................................................................................................................................... Protein_Len: 46 Strand: + Start: 112 End: 249 ............................................................................................................... M I R S T I P I P G G K Q H N K Q N Y R H Y R C N R S I R C Y R I R F I K S K K R Q R Y R P . Protein_Len: 33 Strand: + Start: 152 End: 250 ....................................................................................................................................................... M I N K T I A I T G A T G Q F G A I A L D L L K A K S V N V I A L Protein_Len: 38 Strand: - Start: 80 End: 193 ............................................................................... F Y T I Y N Y N S N F L V T L W G L V L L F V V Y Y V F S D G N C T C C T M ......................................................... Protein_Len: 36 Strand: - Start: 1 End: 108 K I E K F I L K N F I F K F K N W F K R K H L Y K E F T L L T I I T A M .............................................................................................................................................. ttttatttccttaaaaatcaatttattaaaaataaatttaaatttattccaaaacttacgtttatgtaagtacttctcaaaagtaagtaatgtaattatagttgctattgaataatacggtcaaccatcccaataccaggaggaaaacaacataataaacaaaactatcgccattacaggtgcaacaggtcaattcggtgctatcgcattagatttattaaaagcaaaaagcgtcaacgttatcgccctt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttttatttccttaaaaatcaatttattaaaaataaatttaaatttattccaaaacttacgtttatgtaagtacttctcaaaagtaagtaatgtaattatagttgctattgaataatacggtcaaccatcccaataccaggaggaaaacaacataataaacaaaactatcgccattacaggtgcaacaggtcaattcggtgctatcgcattagatttattaaaagcaaaaagcgtcaacgttatcgccctt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01494:AA01495|AA01494:AA01495:conserved hypothetical protein:probable oxidoreductase:->->:1003982..1004231 250 ttttatttccttaaaaatcaatttattaaaaataaatttaaatttattccaaaacttacgtttatgtaagtacttctcaaaagtaagtaatgtaattatagttgctattgaataatacggtcaaccatcccaataccaggaggaaaacaacataataaacaaaactatcgccattacaggtgcaacaggtcaattcggtgctatcgcattagatttattaaaagcaaaaagcgtcaacgttatcgccctt Intra-Species Hit: Count: 1 Min: 55 Max: 250 Len: 196 Subject: aact_AA01494_AA01495|conserved hypothetical protein:probable oxidoreductase|POSITIVE:POSITIVE|[1003982,1004231]|250 HSP 1 e-value: 1.0E-107 bit: 389.0 Len: 196 Query Start:55 Query End:250 Subject Strand: POSITIVE Subject Start: 55 Subject End: 250 ......................................................cttacgtttatgtaagtacttctcaaaagtaagtaatgtaattatagttgctattgaataatacggtcaaccatcccaataccaggaggaaaacaacataataaacaaaactatcgccattacaggtgcaacaggtcaattcggtgctatcgcattagatttattaaaagcaaaaagcgtcaacgttatcgccctt ......................................................cttacgtttatgtaagtacttctcaaaagtaagtaatgtaattatagttgctattgaataatacggtcaaccatcccaataccaggaggaaaacaacataataaacaaaactatcgccattacaggtgcaacaggtcaattcggtgctatcgcattagatttattaaaagcaaaaagcgtcaacgttatcgccctt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUUACGUUUAUGUAAGUACUUCUCAAAAGUAAGUAAUGUAAUUAUAGUUGCUAUUGAAUAAUACGGUCAACCAUCCCAAUACCAGGAGGAAAACAACAUAAUAAACAAAACUAUCGCCAUUACAGGUGCAACAGGUCAAUUCGGUGCUAUCGCAUUAGAUUUAUUAAAAGCAAAAAGCGUCAACGUUAUCGCCCUU ...(((((.((((...(((((((....)).)))))............(((((.((((.......(..(..((.(((........))))).........................((((......)))).....)..).(((..((((....))))..)))...)))).)))))...)))).))))).......... (-32.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGGGCGAUAACGUUGACGCUUUUUGCUUUUAAUAAAUCUAAUGCGAUAGCACCGAAUUGACCUGUUGCACCUGUAAUGGCGAUAGUUUUGUUUAUUAUGUUGUUUUCCUCCUGGUAUUGGGAUGGUUGACCGUAUUAUUCAAUAGCAACUAUAAUUACAUUACUUACUUUUGAGAAGUACUUACAUAAACGUAAG ((((((((.((((....)).)).))))))))......(((..((((((((((......))..))))))))...((((((...(((((((((..((.((((..(((..((((((......)))).))..))))))).))..)))....)))))).....)))))).........)))....(((((......))))) (-40.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 373 156 112 198 150 aact:AA02162|5end_lipoprotein_1478950..1479180_POSITIVE 328 153 113 215 177 aact:AA01054|5end_conserved_hypothetical_protein_733678..733908_POSITIVE 327 160 114 229 170 aact:AA02442|5end_thioredoxin-family_protein_1685704..1685934_POSITIVE 322 156 111 171 128 aact:AA02196|5end_oxaloacetate_decarboxylase_beta_chain_1496308..1496538_POSITIVE 322 188 141 196 139 aact:AA00741|5end_possible_2-oxoglutarate/malate_transporter_506659..506889_POSITIVE 315 170 124 62 15 aact:AA02154|5end_possible_ATP-binding_protein_of_ABC_transporter_1473543..1473773_POSITIVE 303 147 107 213 162 aact:AA02030|5end_threonyl-tRNA_synthetase_1382171..1382401_POSITIVE 301 154 118 37 1 aact:AA01408|5end_conserved_hypothetical_protein_950191..950421_POSITIVE 297 156 115 159 113 aact:AA00194|5end_oxygen-independent_coproporphyrinogen_III_oxidase_135545..135775_POSITIVE 296 153 114 217 174 aact:AA01995|5end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355120..1355350_POSITIVE 296 153 114 56 13 aact:AA01994|5end_chorismate_mutase;_prephenate_dehydrogenase_1354959..1355189_POSITIVE 295 153 115 186 147 aact:AA00838|5end_UDP-N-acetylmuramoylalanine--D-glutamate_ligase_577522..577752_POSITIVE 290 153 115 179 139 aact:AA01760|5end_hydrogenase_isoenzymes_formation_protein_1189787..1190017_POSITIVE 289 160 113 79 24 aact:AA02526|5end_conserved_hypothetical_protein_1755225..1755455_POSITIVE 289 158 116 129 87 aact:AA01681|5end_molybdate-binding_periplasmic_protein_1135159..1135389_POSITIVE 289 158 114 185 135 aact:AA01062|5end_ribonuclease_PH_(RNase_PH)_735706..735936_POSITIVE 289 152 111 65 25 aact:AA00713|5end_chaperone_protein_hscA,_heat_shock_cognate_66_488770..489000_POSITIVE 288 154 116 46 5 aact:AA01547|5end_deoxyribose-phosphate_aldolase_1036271..1036501_POSITIVE 285 152 111 161 107 aact:AA00935|5end_ribose_ABC_transporter,_permease_protein,_N-terminal_645781..646011_POSITIVE 284 147 110 57 18 aact:AA02683|5end_hydrogenase_4_Fe-S_subunit_1868882..1869112_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 373 156 112 97 49 aact:AA02160|3end_conserved_hypothetical_protein_1478929..1479079_POSITIVE 315 170 124 59 12 aact:AA02153|3end_iron(III)_ABC_transporter,_permease_protein_1473620..1473770_POSITIVE 297 156 115 79 33 aact:AA00191|3end_conserved_hypothetical_protein_(possible_acetyltransferase,_GNAT_family)_135545..135695_POSITIVE 291 90 49 128 92 aact:AA01260|3end_hypothetical_protein_855758..855908_POSITIVE 289 160 113 76 21 aact:AA02525|3end_conserved_hypothetical_protein_1755302..1755452_POSITIVE 289 158 116 126 84 aact:AA01679|3end_aspartate-semialdehyde_dehydrogenase;_PTS_system_enzyme_II_A_component_1135236..1135386_POSITIVE 289 152 111 41 1 aact:AA00712|3end_chaperone_protein_hscB,_heat_shock_cognate_488826..488976_POSITIVE 285 152 111 142 88 aact:AA00934|3end_ribose_ABC_transporter,_ATP_binding_protein_645842..645992_POSITIVE 283 155 116 148 97 aact:AA01446|3end_conserved_hypothetical_protein_970326..970476_POSITIVE 282 157 115 88 46 aact:AA01829|3end_hypothetical_protein_1231662..1231812_POSITIVE 277 147 111 111 67 aact:AA02866|3end_lactoylglutathione_lyase_2013108..2013258_POSITIVE 277 160 115 73 28 aact:AA00443|3end_hypothetical_protein_308796..308946_POSITIVE 269 90 49 143 92 aact:AA00537|3end_conserved_hypothetical_protein_368779..368929_POSITIVE 263 146 101 113 63 aact:AA02135|3end_hypothetical_protein_1459067..1459217_POSITIVE 263 146 101 129 79 aact:AA02135.1|3end_hypothetical_protein_1459083..1459233_POSITIVE 262 158 111 83 34 aact:AA01053|3end_conserved_hypothetical_protein_733747..733897_POSITIVE 261 165 124 92 47 aact:AA02280|3end_conserved_hypothetical_protein_1552767..1552917_POSITIVE 259 155 115 130 87 aact:AA01155|3end_hypothetical_protein_791411..791561_POSITIVE 258 153 111 147 109 aact:AA00340|3end_NADP(H)_oxidoreductase_(NAD(P)H_dehydrogenase_(quinone))_229425..229575_POSITIVE 257 154 115 37 1 aact:AA00740|3end_citrate_lyase_related_biosynthesis_protein_(2-(5'-triphosphoribosyl)-3'-dephosphocoenzyme-A_synthase)_506749..506899_POSITIVE