Origin IGS: aacttttaaaccttaaaacggagcaattatacccttgaaaaaatgagattcaactcaaaagcactcaaaaagtggtaaatgaactacaataaaagatagttaacagaaatttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaatttctgttaactatcttttattgtagttcatttaccactttttgagtgcttttgagttgaatctcattttttcaagggtataattgctccgttttaaggtttaaaagtt Mask Tandem Repeat Region ================================================ aacttttaaaccttaaaacggagcaattatacccttgaaaaaatgagattcaactcaaaagcactcaaaaagtggtaaatgaactacaataaaagatagttaacagaaatttta Find is-nt database================================================ Query_seq: AA01491:AA01492|AA01491:AA01492:conserved hypothetical protein:glucose inhibited division protein B:->->:1002832..1002945 114 aacttttaaaccttaaaacggagcaattatacccttgaaaaaatgagattcaactcaaaagcactcaaaaagtggtaaatgaactacaataaaagatagttaacagaaatttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01491:AA01492|AA01491:AA01492:conserved hypothetical protein:glucose inhibited division protein B:->->:1002832..1002945 114 aacttttaaaccttaaaacggagcaattatacccttgaaaaaatgagattcaactcaaaagcactcaaaaagtggtaaatgaactacaataaaagatagttaacagaaatttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01491:AA01492|AA01491:AA01492:conserved hypothetical protein:glucose inhibited division protein B:->->:1002832..1002945 114 aacttttaaaccttaaaacggagcaattatacccttgaaaaaatgagattcaactcaaaagcactcaaaaagtggtaaatgaactacaataaaagatagttaacagaaatttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aacttttaaaccttaaaacggagcaattatacccttgaaaaaatgagattcaactcaaaagcactcaaaaagtggtaaatgaactacaataaaagatagttaacagaaatttta Predict ORF larger than 30AA ================================================ aacttttaaaccttaaaacggagcaattatacccttgaaaaaatgagattcaactcaaaagcactcaaaaagtggtaaatgaactacaataaaagatagttaacagaaatttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aacttttaaaccttaaaacggagcaattatacccttgaaaaaatgagattcaactcaaaagcactcaaaaagtggtaaatgaactacaataaaagatagttaacagaaatttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01491:AA01492|AA01491:AA01492:conserved hypothetical protein:glucose inhibited division protein B:->->:1002832..1002945 114 aacttttaaaccttaaaacggagcaattatacccttgaaaaaatgagattcaactcaaaagcactcaaaaagtggtaaatgaactacaataaaagatagttaacagaaatttta Intra-Species Hit: Count: 1 Min: 1 Max: 114 Len: 114 Subject: aact_AA01491_AA01492|conserved hypothetical protein:glucose inhibited division protein B|POSITIVE:POSITIVE|[1002832,1002945]|114 HSP 1 e-value: 1.0E-58 bit: 226.0 Len: 114 Query Start:1 Query End:114 Subject Strand: POSITIVE Subject Start: 1 Subject End: 114 aacttttaaaccttaaaacggagcaattatacccttgaaaaaatgagattcaactcaaaagcactcaaaaagtggtaaatgaactacaataaaagatagttaacagaaatttta aacttttaaaccttaaaacggagcaattatacccttgaaaaaatgagattcaactcaaaagcactcaaaaagtggtaaatgaactacaataaaagatagttaacagaaatttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACUUUUAAACCUUAAAACGGAGCAAUUAUACCCUUGAAAAAAUGAGAUUCAACUCAAAAGCACUCAAAAAGUGGUAAAUGAACUACAAUAAAAGAUAGUUAACAGAAAUUUUA ..((((((..............((..(((......))).....((((......))))...)).........(((((......)))))..))))))................... (-12.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAUUUCUGUUAACUAUCUUUUAUUGUAGUUCAUUUACCACUUUUUGAGUGCUUUUGAGUUGAAUCUCAUUUUUUCAAGGGUAUAAUUGCUCCGUUUUAAGGUUUAAAAGUU ........((.((((..(((((....((.(((.....((((.((...((((......((((......))))....)))))))))).....))).))....))))))))).)).. (-14.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 253 75 33 231 190 aact:AA02789|5end_oxidoreductase-dehydrogenase_1956641..1956871_POSITIVE 248 87 43 183 145 aact:AA01153|5end_sec-independent_protein_translocase_790166..790396_POSITIVE 220 66 28 224 182 aact:AA00529|5end_outer_membrane_protein,_Omp_18/16_361730..361960_POSITIVE 219 76 31 189 138 aact:AA02724|5end_seryl-tRNA_ligase_1906357..1906587_POSITIVE 219 76 31 95 44 aact:AA02723|5end_hypothetical_protein_1906263..1906493_POSITIVE 216 46 3 171 131 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 213 49 3 167 118 aact:AA02087|5end_1,4-alpha-glucan_branching_enzyme_1418934..1419164_POSITIVE 204 60 20 188 135 aact:AA01249|5end_conserved_hypothetical_protein_(possible_phosphoesterase)_849987..850217_POSITIVE 192 66 23 100 54 aact:AA02349|5end_dihydrodipicolinate_synthetase_1607391..1607621_POSITIVE 192 90 47 94 39 aact:AA01684|5end_ABC_transporter,_ATP-binding_protein;_possible_ATP-binding_component_of_sulfate_permease_A_protein_1136302..1136532_POSITIVE 192 60 18 59 18 aact:AA00816|5end_lacZ_expression_regulator_560900..561130_POSITIVE 186 83 41 81 49 aact:AA02359|5end_hypothetical_protein_1618100..1618330_POSITIVE 185 77 57 131 108 aact:AA01690|5end_penicillin-binding_protein_1C_1146708..1146938_POSITIVE 185 72 49 208 183 aact:AA01607|5end_oligopeptide_transporter,_periplasmic-binding_protein_1085644..1085874_POSITIVE 183 88 49 224 191 aact:AA00747|5end_UTP--hexose-1-phosphate_uridylyltransferase_/_UDPglucose-hexose-1-phosphate_uridylyltransferas_512384..512614_POSITIVE 183 60 12 119 64 aact:AA00443|5end_hypothetical_protein_307601..307831_POSITIVE 182 64 23 195 153 aact:AA02550|5end_iron_(chelated)_ABC_transporter,_ATP-binding_protein_1776751..1776981_POSITIVE 180 75 31 106 59 aact:AA01641|5end_hypothetical_protein_1110819..1111049_POSITIVE 179 108 61 91 43 aact:AA02601|5end_ATP-dependent_helicase_1811397..1811627_POSITIVE 178 93 52 84 43 aact:AA02521|5end_conserved_hypothetical_protein_1752603..1752833_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 252 90 41 111 49 aact:AA01136|3end_hypothetical_protein_778502..778652_POSITIVE 248 87 43 142 104 aact:AA01152|3end_sec-independent_protein_translocase_protein_790205..790355_POSITIVE 236 75 37 127 85 aact:AA01113|3end_transcriptional_regulator_(merR_family)_765249..765399_POSITIVE 220 66 28 117 75 aact:AA00528|3end_conserved_hypothetical_protein_361703..361853_POSITIVE 219 76 31 104 53 aact:AA02722|3end_cytidine_deaminase_1906352..1906502_POSITIVE 204 60 20 65 12 aact:AA01247|3end_hypothetical_protein_849944..850094_POSITIVE 186 83 41 50 18 aact:AA02358|3end_homoserine_kinase_1618149..1618299_POSITIVE 185 72 49 138 113 aact:AA01605|3end_hypothetical_protein_1085654..1085804_POSITIVE 183 60 12 119 64 aact:AA00442|3end_conserved_hypothetical_protein_(possible_zinc_finger_protein)_307681..307831_POSITIVE 179 108 61 85 37 aact:AA02600|3end_conserved_hypothetical_protein_1811471..1811621_POSITIVE 177 76 32 145 96 aact:AA00745|3end_galactokinase_512394..512544_POSITIVE 175 47 12 80 43 aact:AA01342|3end_conserved_hypotheticl_protein_(possible_ribosomal_RNA_adenine_dimethylase)_911085..911235_POSITIVE 174 82 57 56 27 aact:AA01056|3end_conserved_hypothetical_protein_734168..734318_POSITIVE 173 77 44 129 96 aact:AA00771|3end_hypothetical_protein_531620..531770_POSITIVE 171 87 56 66 36 aact:AA01835|3end_conserved_hypothetical_protein_1235389..1235539_POSITIVE 170 84 47 142 99 aact:AA01682|3end_molybdate_ABC_transporter_protein_1136442..1136592_POSITIVE 170 26 2 88 67 aact:AA01057|3end_DnaJ-like_protein_djlA_735047..735197_POSITIVE 168 81 41 93 49 aact:AA01288|3end_hypothetical_protein_872288..872438_POSITIVE 166 45 10 51 12 aact:AA02560|3end_integral_membrane_protein_1783630..1783780_POSITIVE 166 40 19 143 121 aact:AA01120|3end_conserved_hypothetical_protein_770320..770470_POSITIVE