Origin IGS: ggtttttcctgactgattacctttttgttggctattatataatatttttacacaggcgaaatcatctataccttatcgatcttccagacaactttgttttaagaaaagtacagagggaaattttaaccatttcgaaaatcagaaataatca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgattatttctgattttcgaaatggttaaaatttccctctgtacttttcttaaaacaaagttgtctggaagatcgataaggtatagatgatttcgcctgtgtaaaaatattatataatagccaacaaaaaggtaatcagtcaggaaaaacc Mask Tandem Repeat Region ================================================ ggtttttcctgactgattacctttttgttggctattatataatatttttacacaggcgaaatcatctataccttatcgatcttccagacaactttgttttaagaaaagtacagagggaaattttaaccatttcgaaaatcagaaataatca Find is-nt database================================================ Query_seq: AA01477:AA01478|AA01477:AA01478:type I restriction enzyme R protein:hypothetical protein:->->:994540..994690 151 ggtttttcctgactgattacctttttgttggctattatataatatttttacacaggcgaaatcatctataccttatcgatcttccagacaactttgttttaagaaaagtacagagggaaattttaaccatttcgaaaatcagaaataatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01477:AA01478|AA01477:AA01478:type I restriction enzyme R protein:hypothetical protein:->->:994540..994690 151 ggtttttcctgactgattacctttttgttggctattatataatatttttacacaggcgaaatcatctataccttatcgatcttccagacaactttgttttaagaaaagtacagagggaaattttaaccatttcgaaaatcagaaataatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01477:AA01478|AA01477:AA01478:type I restriction enzyme R protein:hypothetical protein:->->:994540..994690 151 ggtttttcctgactgattacctttttgttggctattatataatatttttacacaggcgaaatcatctataccttatcgatcttccagacaactttgttttaagaaaagtacagagggaaattttaaccatttcgaaaatcagaaataatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ggtttttcctgactgattacctttttgttggctattatataatatttttacacaggcgaaatcatctataccttatcgatcttccagacaactttgttttaagaaaagtacagagggaaattttaaccatttcgaaaatcagaaataatca Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 44 End: 145 ........................................... M F T Q A K S S I P Y R S S R Q L C F K K S T E G N F N H F E N Q K ...... Protein_Len: 47 Strand: + Start: 9 End: 149 ........ M T D Y L F V G Y Y I I F L H R R N H L Y L I D L P D N F V L R K V Q R E I L T I S K I R N N .. ggtttttcctgactgattacctttttgttggctattatataatatttttacacaggcgaaatcatctataccttatcgatcttccagacaactttgttttaagaaaagtacagagggaaattttaaccatttcgaaaatcagaaataatca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ggtttttcctgactgattacctttttgttggctattatataatatttttacacaggcgaaatcatctataccttatcgatcttccagacaactttgttttaagaaaagtacagagggaaattttaaccatttcgaaaatcagaaataatca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01477:AA01478|AA01477:AA01478:type I restriction enzyme R protein:hypothetical protein:->->:994540..994690 151 ggtttttcctgactgattacctttttgttggctattatataatatttttacacaggcgaaatcatctataccttatcgatcttccagacaactttgttttaagaaaagtacagagggaaattttaaccatttcgaaaatcagaaataatca Intra-Species Hit: Count: 1 Min: 1 Max: 151 Len: 151 Subject: aact_AA01477_AA01478|type I restriction enzyme R protein:hypothetical protein|POSITIVE:POSITIVE|[994540,994690]|151 HSP 1 e-value: 1.0E-80 bit: 299.0 Len: 151 Query Start:1 Query End:151 Subject Strand: POSITIVE Subject Start: 1 Subject End: 151 ggtttttcctgactgattacctttttgttggctattatataatatttttacacaggcgaaatcatctataccttatcgatcttccagacaactttgttttaagaaaagtacagagggaaattttaaccatttcgaaaatcagaaataatca ggtttttcctgactgattacctttttgttggctattatataatatttttacacaggcgaaatcatctataccttatcgatcttccagacaactttgttttaagaaaagtacagagggaaattttaaccatttcgaaaatcagaaataatca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGUUUUUCCUGACUGAUUACCUUUUUGUUGGCUAUUAUAUAAUAUUUUUACACAGGCGAAAUCAUCUAUACCUUAUCGAUCUUCCAGACAACUUUGUUUUAAGAAAAGUACAGAGGGAAAUUUUAACCAUUUCGAAAAUCAGAAAUAAUCA ((((((.((((..((((..((........))..))))..(((.....)))..)))).)))))).......((((..(..((((..(((((....))))).))))...)....))))............(((((........)))))..... (-20.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUUAUUUCUGAUUUUCGAAAUGGUUAAAAUUUCCCUCUGUACUUUUCUUAAAACAAAGUUGUCUGGAAGAUCGAUAAGGUAUAGAUGAUUUCGCCUGUGUAAAAAUAUUAUAUAAUAGCCAACAAAAAGGUAAUCAGUCAGGAAAAACC ((((((((((........))))))))))...(((((.((((((((((((((...(((....)))....)))).....))))))))))(((((..((((.(((.....((((....))))....)))...)))))))))....))))).... (-29.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 275 90 53 173 142 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 260 91 51 56 17 aact:AA00513|5end_pseudouridylate_synthase_(pseudouridine_synthase)_354950..355180_POSITIVE 255 96 53 51 4 aact:AA01062|5end_ribonuclease_PH_(RNase_PH)_735706..735936_POSITIVE 236 86 50 161 132 aact:AA02090|5end_glucose-1-phosphate_adenylyltransferase_1423162..1423392_POSITIVE 231 91 51 185 138 aact:AA00194|5end_oxygen-independent_coproporphyrinogen_III_oxidase_135545..135775_POSITIVE 223 94 52 158 114 aact:AA02025|5end_hypothetical_protein_1378667..1378897_POSITIVE 213 96 55 47 1 aact:AA01755|5end_cobalt_membrane_transport_protein_1185825..1186055_POSITIVE 212 95 51 62 19 aact:AA00715|5end_[2Fe-2S]_ferredoxin_490632..490862_POSITIVE 211 31 1 210 175 aact:AA00061|5end_cytochrome_c-type_biogenesis_ATP-binding_protein_46082..46312_POSITIVE 210 100 54 116 82 aact:AA00342|5end_hypothetical_protein_229695..229925_POSITIVE 208 41 7 94 57 aact:AA01767|5end_conserved_hypothetical_protein_1192528..1192758_POSITIVE 208 77 42 48 15 aact:AA00325|5end_conserved_hypothetical_protein_221314..221544_POSITIVE 207 59 11 223 184 aact:AA02902|5end_aerobic_respiration_control_protein_2031171..2031401_POSITIVE 207 84 52 188 156 aact:AA01792|5end_conserved_hypothetical_protein_1208058..1208288_POSITIVE 205 100 51 82 33 aact:AA02075|5end_conserved_hypothetical_protein_1409654..1409884_POSITIVE 204 117 78 63 29 aact:AA03016|5end_hypothetical_protein_2105144..2105374_POSITIVE 203 78 31 205 145 aact:AA01825|5end_iron(III)_ABC_transporter,_permease_protein_1228475..1228705_POSITIVE 203 95 53 61 24 aact:AA01499|5end_fructose_1,6-bisphosphatase_protein_1008357..1008587_POSITIVE 203 66 26 73 30 aact:AA01308|5end_cyclic_AMP_receptor_protein_888044..888274_POSITIVE 202 58 20 88 49 aact:AA00382|5end_hypothetical_protein_258593..258823_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 236 86 50 131 102 aact:AA02089|3end_glycogen_operon_protein_1423212..1423362_POSITIVE 235 59 11 140 82 aact:AA00221|3end_NADP-specific_glutamate_dehydrogenase_153865..154015_POSITIVE 231 91 51 105 58 aact:AA00191|3end_conserved_hypothetical_protein_(possible_acetyltransferase,_GNAT_family)_135545..135695_POSITIVE 223 94 52 150 106 aact:AA02024|3end_acyl_carrier_protein_phosphodiesterase_1378739..1378889_POSITIVE 221 88 55 59 22 aact:AA00227|3end_hypothetical_sequence_156642..156792_POSITIVE 217 96 65 38 5 aact:AA01059|3end_orotate_phosphoribosyltransferase_735773..735923_POSITIVE 212 95 51 47 4 aact:AA00713|3end_chaperone_protein_hscA,_heat_shock_cognate_66_490697..490847_POSITIVE 211 87 55 78 46 aact:AA01567|3end_conserved_hypothetical_protein_(possible_cell-wall-associated_hydrolase)_1055215..1055365_POSITIVE 210 100 54 144 110 aact:AA00341|3end_hypothetical_protein_229803..229953_POSITIVE 209 140 107 111 75 aact:AA01614|3end_D-isomer_specific_2-hydroxyacid_dehydrogenase_family_protein_1090719..1090869_POSITIVE 208 41 7 122 85 aact:AA01765|3end_Mu-like_prophage_FluMu_transposase_A_1192636..1192786_POSITIVE 207 59 11 110 71 aact:AA02899|3end_oligopeptide_ABC_transporter,_ATP-binding_protein_2031138..2031288_POSITIVE 204 117 78 90 56 aact:AA03015|3end_hypothetical_protein_2105251..2105401_POSITIVE 200 88 41 64 26 aact:AA01242|3end_DNA-directed_RNA_polymerase_alpha_subunit_847009..847159_POSITIVE 199 57 11 98 42 aact:AA01357|3end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919785..919935_POSITIVE 196 31 1 99 72 aact:AA02094|3end_glycogen_phosphorylase;_glycogen_synthase_1428778..1428928_POSITIVE 195 34 2 89 63 aact:AA02237|3end_molybdopterin_biosynthesis_protein_E_chain_1523292..1523442_POSITIVE 195 91 51 47 3 aact:AA01842|3end_conserved_hypothetical_protein_1238467..1238617_POSITIVE 194 31 1 40 9 aact:AA00527|3end_ATP-dependent_DNA_helicase_361463..361613_POSITIVE 193 32 2 103 63 aact:AA01645|3end_ferric_transport_ABC-related_ATP-binding_protein_1115184..1115334_POSITIVE