Origin IGS: actaaagtgcggtgctttttctttatgttttttataaaaatgtgatcctgatcacataaaatctactaaaaagcaccgagcttaaacgaatgagttgtcggtttttaggtagaatggtaaacgatacaactatctttaaggagaccaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttggtctccttaaagatagttgtatcgtttaccattctacctaaaaaccgacaactcattcgtttaagctcggtgctttttagtagattttatgtgatcaggatcacatttttataaaaaacataaagaaaaagcaccgcactttagt Mask Tandem Repeat Region ================================================ actaaagtgcggtgctttttctttatgttttttataaaaatgtgatcctgatcacataaaatctactaaaaagcaccgagcttaaacgaatgagttgtcggtttttaggtagaatggtaaacgatacaactatctttaaggagaccaa Find is-nt database================================================ Query_seq: AA01464:AA01465|AA01464:AA01465:hypothetical protein:mannose-specific phosphotransferase element:->->:984464..984611 148 actaaagtgcggtgctttttctttatgttttttataaaaatgtgatcctgatcacataaaatctactaaaaagcaccgagcttaaacgaatgagttgtcggtttttaggtagaatggtaaacgatacaactatctttaaggagaccaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01464:AA01465|AA01464:AA01465:hypothetical protein:mannose-specific phosphotransferase element:->->:984464..984611 148 actaaagtgcggtgctttttctttatgttttttataaaaatgtgatcctgatcacataaaatctactaaaaagcaccgagcttaaacgaatgagttgtcggtttttaggtagaatggtaaacgatacaactatctttaaggagaccaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01464:AA01465|AA01464:AA01465:hypothetical protein:mannose-specific phosphotransferase element:->->:984464..984611 148 actaaagtgcggtgctttttctttatgttttttataaaaatgtgatcctgatcacataaaatctactaaaaagcaccgagcttaaacgaatgagttgtcggtttttaggtagaatggtaaacgatacaactatctttaaggagaccaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 actaaagtgcggtgctttttctttatgttttttataaaaatgtgatcctgatcacataaaatctactaaaaagcaccgagcttaaacgaatgagttgtcggtttttaggtagaatggtaaacgatacaactatctttaaggagaccaa Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: - Start: 35 End: 148 .................................. L F T I R I V Y F R S F L V S S L R I L Q R N K P L I T F S V V I K L S V M actaaagtgcggtgctttttctttatgttttttataaaaatgtgatcctgatcacataaaatctactaaaaagcaccgagcttaaacgaatgagttgtcggtttttaggtagaatggtaaacgatacaactatctttaaggagaccaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ actaaagtgcggtgctttttctttatgttttttataaaaatgtgatcctgatcacataaaatctactaaaaagcaccgagcttaaacgaatgagttgtcggtttttaggtagaatggtaaacgatacaactatctttaaggagaccaa Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 77 HP_score: -6.2 Tail_Score: -4.82901 Start: 41 End: 56 Full_Region: ttttagtagatttta tgtgat cagg atcaca tttttataaaaaaca ........................................tgtgatcaggatcaca............................................................................................ Find igs database================================================ Query_seq: AA01464:AA01465|AA01464:AA01465:hypothetical protein:mannose-specific phosphotransferase element:->->:984464..984611 148 actaaagtgcggtgctttttctttatgttttttataaaaatgtgatcctgatcacataaaatctactaaaaagcaccgagcttaaacgaatgagttgtcggtttttaggtagaatggtaaacgatacaactatctttaaggagaccaa Intra-Species Hit: Count: 1 Min: 1 Max: 148 Len: 148 Subject: aact_AA01464_AA01465|hypothetical protein:mannose-specific phosphotransferase element|POSITIVE:POSITIVE|[984464,984611]|148 HSP 1 e-value: 8.0E-79 bit: 293.0 Len: 148 Query Start:1 Query End:148 Subject Strand: POSITIVE Subject Start: 1 Subject End: 148 actaaagtgcggtgctttttctttatgttttttataaaaatgtgatcctgatcacataaaatctactaaaaagcaccgagcttaaacgaatgagttgtcggtttttaggtagaatggtaaacgatacaactatctttaaggagaccaa actaaagtgcggtgctttttctttatgttttttataaaaatgtgatcctgatcacataaaatctactaaaaagcaccgagcttaaacgaatgagttgtcggtttttaggtagaatggtaaacgatacaactatctttaaggagaccaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACUAAAGUGCGGUGCUUUUUCUUUAUGUUUUUUAUAAAAAUGUGAUCCUGAUCACAUAAAAUCUACUAAAAAGCACCGAGCUUAAACGAAUGAGUUGUCGGUUUUUAGGUAGAAUGGUAAACGAUACAACUAUCUUUAAGGAGACCAA ....((((.(((((((((((.((((((.....)))))).(((((((....)))))))..........))))))))))).)))).(((......)))...(((((((((((((....(((.....)))..))))))....))))))).. (-38.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGGUCUCCUUAAAGAUAGUUGUAUCGUUUACCAUUCUACCUAAAAACCGACAACUCAUUCGUUUAAGCUCGGUGCUUUUUAGUAGAUUUUAUGUGAUCAGGAUCACAUUUUUAUAAAAAACAUAAAGAAAAAGCACCGCACUUUAGU ........((((((((.((((((...((((..............))))..))))))...)).))))))..(((((((((((..((..(((((((((((....)))))))........))))..))...)))))))))))......... (-31.04) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 278 104 65 134 90 aact:AA02378|5end_hypothetical_protein_1627753..1627983_POSITIVE 275 47 2 151 91 aact:AA01286|5end_5,10-methylenetetrahydrofolate_dehydrogenase_(bifunctional_protein)_870309..870539_POSITIVE 272 116 73 130 88 aact:AA02188|5end_hypothetical_protein_1492785..1493015_POSITIVE 272 50 3 151 102 aact:AA01918|5end_anaerobic_C4-dicarboxylate_transporter_1290931..1291161_POSITIVE 271 50 1 166 109 aact:AA02014|5end_probable_hemolysin_1370406..1370636_POSITIVE 269 35 4 134 103 aact:AA01954|5end_excinuclease_ABC,_subunit_C_1320468..1320698_POSITIVE 267 35 4 102 71 aact:AA00065|5end_hypothetical_protein_48854..49084_POSITIVE 266 47 2 71 19 aact:AA01590|5end_hypothetical_protein_1070766..1070996_POSITIVE 266 45 3 168 131 aact:AA01471|5end_type_I_restriction_enzyme_M_(modification_chain)_987321..987551_POSITIVE 264 50 4 228 184 aact:AA02774|5end_collagenase_prtC_homolog,_possible_protease_1936870..1937100_POSITIVE 264 50 4 57 13 aact:AA02773|5end_hypothetical_protein_1936699..1936929_POSITIVE 263 117 73 58 12 aact:AA00950|5end_conserved_hypothetical_protein_656922..657152_POSITIVE 262 120 74 50 2 aact:AA02704|5end_dihydrolipoamide_succinyltransferase_1885495..1885725_POSITIVE 262 111 72 178 130 aact:AA02044|5end_phosphotransferase_system_enzyme_II,_galactitol_specific,_protein_A_1391488..1391718_POSITIVE 260 138 91 206 165 aact:AA01546|5end_deoxyribose_operon_repressor_1035494..1035724_POSITIVE 258 31 5 189 163 aact:AA01401|5end_3-deoxy-D-manno-octulosonic-acid_transferase_946165..946395_POSITIVE 258 99 51 190 140 aact:AA00918|5end_hypothetical_protein_630044..630274_POSITIVE 257 49 2 190 151 aact:AA01173|5end_VapD-homolog_803850..804080_POSITIVE 257 21 4 192 175 aact:AA00004|5end_conserved_hypothetical_protein_(possible_methyltransferase)_3463..3693_POSITIVE 256 118 73 216 174 aact:AA00889|5end_conserved_hypothetical_protein_616857..617087_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 310 112 74 151 109 aact:AA00977|3end_transcriptional_regulator_(LysR_family)_683334..683484_POSITIVE 272 116 73 112 70 aact:AA02187|3end_hypothetical_protein_1492847..1492997_POSITIVE 272 50 3 136 87 aact:AA01917|3end_hypothetical_protein_1290996..1291146_POSITIVE 267 35 4 114 83 aact:AA00064|3end_ribosomal_small_subunit_pseudouridine_synthase_48946..49096_POSITIVE 266 47 2 96 44 aact:AA01589|3end_conserved_hypothetical_protein_1070871..1071021_POSITIVE 264 50 4 54 10 aact:AA02772|3end_hypothetical_protein_1936776..1936926_POSITIVE 263 104 67 43 7 aact:AA02375|3end_D-3-phosphoglycerate_dehydrogenase_1627742..1627892_POSITIVE 262 120 74 57 9 aact:AA02703|3end_hypothetical_protein_1885582..1885732_POSITIVE 260 138 91 65 24 aact:AA01544|3end_conserved_hypothetical_protein_1035433..1035583_POSITIVE 259 50 4 54 1 aact:AA02013|3end_carbonic_anhydrase_1370374..1370524_POSITIVE 257 88 41 95 32 aact:AA01611|3end_anthranilate_synthase_component_II_1089619..1089769_POSITIVE 257 49 2 107 68 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 257 110 68 139 101 aact:AA00114|3end_peptidyl-prolyl_cis-trans_isomerase_D_(PPIase_D)_(rotamase_D)_83971..84121_POSITIVE 257 21 4 139 122 aact:AA00003|3end_DNA_replication_and_repair_protein_3490..3640_POSITIVE 256 118 73 134 92 aact:AA00886|3end_hypothetical_protein_616855..617005_POSITIVE 253 78 40 81 34 aact:AA01464|3end_hypothetical_protein_984403..984553_POSITIVE 251 48 3 108 61 aact:AA02978|3end_sufI_protein_precursor_homolog_2083308..2083458_POSITIVE 251 118 75 125 75 aact:AA01057|3end_DnaJ-like_protein_djlA_735047..735197_POSITIVE 249 50 4 47 10 aact:AA01663|3end_GTP_cyclohydrolase_I_1128204..1128354_POSITIVE 247 79 36 51 3 aact:AA01395|3end_conserved_hypothetical_protein_943737..943887_POSITIVE