Origin IGS: aatctgcacctcaaattaggtgtccgggttttggggggcagatcataggtgtttataaggaaaataaccgtttttcattaattcaaagtttagcaatagcctacctgtcgcccgttgccatca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgatggcaacgggcgacaggtaggctattgctaaactttgaattaatgaaaaacggttattttccttataaacacctatgatctgccccccaaaacccggacacctaatttgaggtgcagatt Mask Tandem Repeat Region ================================================ aatctgcacctcaaattaggtgtccgggttttggggggcagatcataggtgtttataaggaaaataaccgtttttcattaattcaaagtttagcaatagcctacctgtcgcccgttgccatca Find is-nt database================================================ Query_seq: AA01445:AA01446|AA01445:AA01446:hypothetical protein:conserved hypothetical protein:->->:970168..970290 123 aatctgcacctcaaattaggtgtccgggttttggggggcagatcataggtgtttataaggaaaataaccgtttttcattaattcaaagtttagcaatagcctacctgtcgcccgttgccatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01445:AA01446|AA01445:AA01446:hypothetical protein:conserved hypothetical protein:->->:970168..970290 123 aatctgcacctcaaattaggtgtccgggttttggggggcagatcataggtgtttataaggaaaataaccgtttttcattaattcaaagtttagcaatagcctacctgtcgcccgttgccatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01445:AA01446|AA01445:AA01446:hypothetical protein:conserved hypothetical protein:->->:970168..970290 123 aatctgcacctcaaattaggtgtccgggttttggggggcagatcataggtgtttataaggaaaataaccgtttttcattaattcaaagtttagcaatagcctacctgtcgcccgttgccatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatctgcacctcaaattaggtgtccgggttttggggggcagatcataggtgtttataaggaaaataaccgtttttcattaattcaaagtttagcaatagcctacctgtcgcccgttgccatca Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 15 End: 122 .............. M R C P G F G G Q I I G V Y K E N N R F S L I Q S L A I A Y L S P V A I . aatctgcacctcaaattaggtgtccgggttttggggggcagatcataggtgtttataaggaaaataaccgtttttcattaattcaaagtttagcaatagcctacctgtcgcccgttgccatca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatctgcacctcaaattaggtgtccgggttttggggggcagatcataggtgtttataaggaaaataaccgtttttcattaattcaaagtttagcaatagcctacctgtcgcccgttgccatca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01445:AA01446|AA01445:AA01446:hypothetical protein:conserved hypothetical protein:->->:970168..970290 123 aatctgcacctcaaattaggtgtccgggttttggggggcagatcataggtgtttataaggaaaataaccgtttttcattaattcaaagtttagcaatagcctacctgtcgcccgttgccatca Intra-Species Hit: Count: 1 Min: 1 Max: 123 Len: 123 Subject: aact_AA01445_AA01446|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[970168,970290]|123 HSP 1 e-value: 6.0E-64 bit: 244.0 Len: 123 Query Start:1 Query End:123 Subject Strand: POSITIVE Subject Start: 1 Subject End: 123 aatctgcacctcaaattaggtgtccgggttttggggggcagatcataggtgtttataaggaaaataaccgtttttcattaattcaaagtttagcaatagcctacctgtcgcccgttgccatca aatctgcacctcaaattaggtgtccgggttttggggggcagatcataggtgtttataaggaaaataaccgtttttcattaattcaaagtttagcaatagcctacctgtcgcccgttgccatca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUCUGCACCUCAAAUUAGGUGUCCGGGUUUUGGGGGGCAGAUCAUAGGUGUUUAUAAGGAAAAUAACCGUUUUUCAUUAAUUCAAAGUUUAGCAAUAGCCUACCUGUCGCCCGUUGCCAUCA .....((((((......))))))...(((..((((.(((((....(((((........(((((((....)))))))...........(.....)....))))).))))).))))..))).... (-34.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUGGCAACGGGCGACAGGUAGGCUAUUGCUAAACUUUGAAUUAAUGAAAAACGGUUAUUUUCCUUAUAAACACCUAUGAUCUGCCCCCCAAAACCCGGACACCUAAUUUGAGGUGCAGAUU ...((((((..(.(........).)..))))))..........(((.(((((.......))))).)))...........(((((((.((.((((....((...))...)))).)).))))))) (-23.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 865 45 1 122 78 aact:AA02115|5end_hypothetical_protein_1444528..1444758_POSITIVE 786 45 1 122 78 aact:AA00008|5end_hypothetical_protein_(possible_transposase_fragment)_5032..5262_POSITIVE 751 50 1 126 78 aact:AA02340|5end_hypothetical_protein_1598887..1599117_POSITIVE 751 50 1 140 92 aact:AA01267|5end_degenerate_transposase_fragment_862267..862497_POSITIVE 751 50 1 126 78 aact:AA01075|5end_hypothetical_protein_745605..745835_POSITIVE 510 45 1 136 92 aact:AA00535|5end_hypothetical_protein_365695..365925_POSITIVE 465 50 1 69 14 aact:AA02419|5end_hypothetical_protein_1661731..1661961_POSITIVE 409 50 1 76 20 aact:AA02681|5end_hypothetical_protein_1865749..1865979_POSITIVE 376 61 21 146 101 aact:AA01287|5end_transposase_871583..871813_POSITIVE 355 120 71 81 33 aact:AA00574|5end_regulatory_protein_392113..392343_POSITIVE 353 50 6 52 1 aact:AA01176|5end_hypothetical_protein_806309..806539_POSITIVE 345 45 21 125 101 aact:AA01444|5end_ISRS011/IS150_related_transposase,_orfB_969159..969389_POSITIVE 332 43 3 164 124 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 327 52 19 49 10 aact:AA02629|5end_hypothetical_protein_1830072..1830302_POSITIVE 321 119 92 159 135 aact:AA00887|5end_hypothetical_protein_616739..616969_POSITIVE 313 51 21 136 106 aact:AA00145|5end_probable_degenerate_transposase_fragment_105046..105276_POSITIVE 302 45 3 198 147 aact:AA01987|5end_hypothetical_protein_1350415..1350645_POSITIVE 301 56 18 150 108 aact:AA02937|5end_conserved_hypothetical_protein_2056379..2056609_POSITIVE 299 70 25 98 55 aact:AA02004|5end_homoserine_O-acetyltransferase_1361937..1362167_POSITIVE 298 57 12 102 52 aact:AA01717|5end_fumarate_hydratase,_class_II_1161189..1161419_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 865 45 1 84 40 aact:AA02114|3end_exodeoxyribonuclease_I_1444570..1444720_POSITIVE 786 45 1 77 33 aact:AA01402|3end_beta_1-4_glucosyltransferase_948382..948532_POSITIVE 786 45 1 65 21 aact:AA00007|3end_superoxide_dismutase_[Cu-Zn]_precursor_5055..5205_POSITIVE 751 50 1 64 16 aact:AA01074|3end_diacylglycerol_kinase_745623..745773_POSITIVE 667 50 6 44 1 aact:AA02338|3end_conserved_hypothetical_protein;_possible_integral_membrane_protein_1598885..1599035_POSITIVE 667 50 6 44 1 aact:AA01266|3end_hypothetical_protein_862251..862401_POSITIVE 486 51 14 35 1 aact:AA00629|3end_inosine_5'_monophosphate_dehydrogenase_(IMP_dehydrogenase)_433469..433619_POSITIVE 465 50 1 69 14 aact:AA02418|3end_peptidyl-tRNA_hydrolase_1661811..1661961_POSITIVE 409 50 1 58 2 aact:AA02679|3end_formate_dehydrogenase,_gamma_subunit_1865811..1865961_POSITIVE 399 50 4 58 5 aact:AA01175|3end_6-phosphogluconate_dehydrogenase_806395..806545_POSITIVE 366 51 19 55 25 aact:AA00221|3end_NADP-specific_glutamate_dehydrogenase_153865..154015_POSITIVE 345 45 21 79 55 aact:AA01442|3end_phosphatidylserine_synthetase_969193..969343_POSITIVE 332 43 3 103 63 aact:AA01289|3end_hypothetical_protein_872848..872998_POSITIVE 323 49 2 76 20 aact:AA00020|3end_conserved_hypothetical_protein_14486..14636_POSITIVE 301 56 18 64 22 aact:AA02936|3end_lipoprotein_E_precursor;_outer_membrane_protein_P4_precursor;_acid_phosphatase_2056373..2056523_POSITIVE 294 116 71 146 99 aact:AA01811|3end_electron_transport_complex_protein_1221033..1221183_POSITIVE 293 50 5 148 95 aact:AA02551|3end_iron_(chelated)_ABC_transporter,_permease_protein_1778575..1778725_POSITIVE 289 43 2 89 48 aact:AA01445|3end_hypothetical_protein_970107..970257_POSITIVE 288 50 1 53 8 aact:AA00382|3end_hypothetical_protein_258885..259035_POSITIVE 287 57 20 100 71 aact:AA02362|3end_conserved_hypothetical_protein_1620725..1620875_POSITIVE