Origin IGS: agtgggtgccgatatggatagcgataaaatgaacggattttgctcatttttagtgtaaaagtgtgagcattataacgaaaaaaaaccttttttctaccaaggtgggcgataaatattgtaaactacggcgctaattaaaattcatttacattcttactcacagcgtttttatagagaactaattt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaattagttctctataaaaacgctgtgagtaagaatgtaaatgaattttaattagcgccgtagtttacaatatttatcgcccaccttggtagaaaaaaggttttttttcgttataatgctcacacttttacactaaaaatgagcaaaatccgttcattttatcgctatccatatcggcacccact Mask Tandem Repeat Region ================================================ agtgggtgccgatatggatagcgataaaatgaacggattttgctcatttttagtgtaaaagtgtgagcattataacgaaaaaaaaccttttttctaccaaggtgggcgataaatattgtaaactacggcgctaattaaaattcatttacattcttactcacagcgtttttatagagaactaattt Find is-nt database================================================ Query_seq: AA01441:AA01442|AA01441:AA01442:tRNA/rRNA methylase:phosphatidylserine synthetase:->->:967704..967888 185 agtgggtgccgatatggatagcgataaaatgaacggattttgctcatttttagtgtaaaagtgtgagcattataacgaaaaaaaaccttttttctaccaaggtgggcgataaatattgtaaactacggcgctaattaaaattcatttacattcttactcacagcgtttttatagagaactaattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01441:AA01442|AA01441:AA01442:tRNA/rRNA methylase:phosphatidylserine synthetase:->->:967704..967888 185 agtgggtgccgatatggatagcgataaaatgaacggattttgctcatttttagtgtaaaagtgtgagcattataacgaaaaaaaaccttttttctaccaaggtgggcgataaatattgtaaactacggcgctaattaaaattcatttacattcttactcacagcgtttttatagagaactaattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01441:AA01442|AA01441:AA01442:tRNA/rRNA methylase:phosphatidylserine synthetase:->->:967704..967888 185 agtgggtgccgatatggatagcgataaaatgaacggattttgctcatttttagtgtaaaagtgtgagcattataacgaaaaaaaaccttttttctaccaaggtgggcgataaatattgtaaactacggcgctaattaaaattcatttacattcttactcacagcgtttttatagagaactaattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agtgggtgccgatatggatagcgataaaatgaacggattttgctcatttttagtgtaaaagtgtgagcattataacgaaaaaaaaccttttttctaccaaggtgggcgataaatattgtaaactacggcgctaattaaaattcatttacattcttactcacagcgtttttatagagaactaattt Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: - Start: 3 End: 116 .. P H R Y P Y R Y F S R I K S M K L T F T H A N Y R F F V K K R G L H A I F M ..................................................................... agtgggtgccgatatggatagcgataaaatgaacggattttgctcatttttagtgtaaaagtgtgagcattataacgaaaaaaaaccttttttctaccaaggtgggcgataaatattgtaaactacggcgctaattaaaattcatttacattcttactcacagcgtttttatagagaactaattt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agtgggtgccgatatggatagcgataaaatgaacggattttgctcatttttagtgtaaaagtgtgagcattataacgaaaaaaaaccttttttctaccaaggtgggcgataaatattgtaaactacggcgctaattaaaattcatttacattcttactcacagcgtttttatagagaactaattt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01441:AA01442|AA01441:AA01442:tRNA/rRNA methylase:phosphatidylserine synthetase:->->:967704..967888 185 agtgggtgccgatatggatagcgataaaatgaacggattttgctcatttttagtgtaaaagtgtgagcattataacgaaaaaaaaccttttttctaccaaggtgggcgataaatattgtaaactacggcgctaattaaaattcatttacattcttactcacagcgtttttatagagaactaattt Intra-Species Hit: Count: 1 Min: 1 Max: 185 Len: 185 Subject: aact_AA01441_AA01442|tRNA/rRNA methylase:phosphatidylserine synthetase|POSITIVE:POSITIVE|[967704,967888]|185 HSP 1 e-value: 2.0E-86 bit: 319.0 Len: 185 Query Start:1 Query End:185 Subject Strand: POSITIVE Subject Start: 1 Subject End: 185 agtgggtgccgatatggatagcgataaaatgaacggattttgctcatttttagtgtaaaagtgtgagcattataacgnnnnnnnnccttttttctaccaaggtgggcgataaatattgtaaactacggcgctaattaaaattcatttacattcttactcacagcgtttttatagagaactaattt agtgggtgccgatatggatagcgataaaatgaacggattttgctcatttttagtgtaaaagtgtgagcattataacgaaaaaaaaccttttttctaccaaggtgggcgataaatattgtaaactacggcgctaattaaaattcatttacattcttactcacagcgtttttatagagaactaattt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUGGGUGCCGAUAUGGAUAGCGAUAAAAUGAACGGAUUUUGCUCAUUUUUAGUGUAAAAGUGUGAGCAUUAUAACGAAAAAAAACCUUUUUUCUACCAAGGUGGGCGAUAAAUAUUGUAAACUACGGCGCUAAUUAAAAUUCAUUUACAUUCUUACUCACAGCGUUUUUAUAGAGAACUAAUUU .(((((((..(((((((((((((...........((....(((((((.(((.......))).))))))).......(((((((....)))))))..))..(((..(((((....)))))..)))....))))).......)))))....)))..)))))))...((((((...))))))...... (-35.31) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUUAGUUCUCUAUAAAAACGCUGUGAGUAAGAAUGUAAAUGAAUUUUAAUUAGCGCCGUAGUUUACAAUAUUUAUCGCCCACCUUGGUAGAAAAAAGGUUUUUUUUCGUUAUAAUGCUCACACUUUUACACUAAAAAUGAGCAAAAUCCGUUCAUUUUAUCGCUAUCCAUAUCGGCACCCACU .....................((((((((((....(((((((....................))))))).........(((......))).(((((((....))))))).......)))))))............(((((((((.......)))))))))...............)))....... (-27.25) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 329 131 97 176 136 aact:AA02850|5end_dATP_pyrophosphohydrolase_2000472..2000702_POSITIVE 300 38 1 92 58 aact:AA00563|5end_elongation_factor_Tu_386507..386737_POSITIVE 295 132 99 175 135 aact:AA02144|5end_iron_(III)_ABC_transporter,_permease_protein_1467583..1467813_POSITIVE 271 131 94 175 144 aact:AA01770|5end_hypothetical_protein_1193111..1193341_POSITIVE 270 130 86 91 40 aact:AA02001|5end_conserved_hypothetical_protein_1359842..1360072_POSITIVE 270 168 124 163 109 aact:AA01941|5end_hypothetical_protein_1312480..1312710_POSITIVE 270 43 15 33 3 aact:AA01794|5end_glycine_cleavage_system_transcription_activator_1209708..1209938_POSITIVE 268 45 5 217 179 aact:AA00426|5end_conserved_hypothetical_protein_288027..288257_POSITIVE 267 134 92 212 176 aact:AA01825|5end_iron(III)_ABC_transporter,_permease_protein_1228475..1228705_POSITIVE 265 49 1 92 45 aact:AA00306|5end_nitrogen_regulatory_protein_p-II_207923..208153_POSITIVE 264 43 2 226 188 aact:AA02158|5end_TonB-dependent_outer_membrane_receptor_1476155..1476385_POSITIVE 262 44 2 121 65 aact:AA01426|5end_threonine_deaminase_(dehydratase)_958207..958437_POSITIVE 260 44 3 189 138 aact:AA00726|5end_apolipoprotein_N-acyltransferase,_copper_498265..498495_POSITIVE 258 49 5 93 35 aact:AA02139|5end_possible_membrane_protein_1461161..1461391_POSITIVE 257 132 97 170 131 aact:AA01750|5end_MTA/SAH_nucleosidase_[Includes:_5'-methylthioadenosine_nucleosidase;_S-adenosylhomocysteine_nucleosidase]_1183750..1183980_POSITIVE 257 41 3 44 1 aact:AA00404|5end_conserved_hypothetical_protein_269822..270052_POSITIVE 255 49 4 216 175 aact:AA02444|5end_hypothetical_protein_1686175..1686405_POSITIVE 255 39 1 55 26 aact:AA02434|5end_membrane_protein_1678547..1678777_POSITIVE 255 47 1 156 97 aact:AA00598|5end_hypothetical_protein_408080..408310_POSITIVE 253 48 1 53 7 aact:AA01274|5end_hypothetical_protein_864519..864749_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 329 131 97 139 99 aact:AA02848|3end_conserved_hypothetical_protein_2000515..2000665_POSITIVE 295 132 99 44 4 aact:AA02142|3end_outer_membrane_protein_OprM_precursor_1467532..1467682_POSITIVE 286 46 3 133 79 aact:AA02873|3end_conserved_hypothetical_protein_2017627..2017777_POSITIVE 271 131 94 116 85 aact:AA01769|3end_hypothetical_protein_1193132..1193282_POSITIVE 270 168 124 143 89 aact:AA01939|3end_NAD(P)_transhydrogenase_subunit_beta_1312540..1312690_POSITIVE 270 43 15 106 76 aact:AA01793|3end_conserved_hypothetical_protein_1209861..1210011_POSITIVE 268 110 66 131 85 aact:AA00468|3end_tryptophan-specific_transport_protein_/_tyrosine-specific_transport_protein_326124..326274_POSITIVE 266 131 99 150 113 aact:AA02143|3end_hypothetical_protein_1467641..1467791_POSITIVE 262 44 2 67 11 aact:AA01424|3end_threonine_deaminase_(dehydratase)_958233..958383_POSITIVE 260 44 3 120 69 aact:AA00725|3end_translation_initiation_factor_1_498276..498426_POSITIVE 259 45 6 151 102 aact:AA00221|3end_NADP-specific_glutamate_dehydrogenase_153865..154015_POSITIVE 257 132 97 150 111 aact:AA01748|3end_thiol:disulfide_interchange_protein_1183810..1183960_POSITIVE 251 46 2 97 47 aact:AA01096|3end_conserved_hypothetical_protei_757282..757432_POSITIVE 251 43 2 125 91 aact:AA00980|3end_hypothetical_protein_685409..685559_POSITIVE 249 129 100 51 24 aact:AA01198|3end_hypothetical_protein_822051..822201_POSITIVE 245 135 94 86 25 aact:AA00605|3end_CDP-diglyceride_pyrophosphorylase_411876..412026_POSITIVE 243 44 3 98 52 aact:AA02958|3end_3-phenylpropionic_acid_transporter_2070491..2070641_POSITIVE 243 129 86 64 17 aact:AA02705|3end_hypothetical_protein_1886928..1887078_POSITIVE 243 133 97 146 110 aact:AA02006|3end_PEP--fructosephosphotransferase_system_repressor,_DeoR_family_1364993..1365143_POSITIVE 243 43 2 66 13 aact:AA01950|3end_ABC_transporter,_membrane_spanning_protein_1319044..1319194_POSITIVE