Origin IGS: acttacgataacttctattttgaggaaggaatttttttactacgtgaaagatttaatcttttattaaaattcaaggaaacaacgaatatttttgtgcaaaatttgttaccattagccatctattttatttgaggtaacaaatc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gatttgttacctcaaataaaatagatggctaatggtaacaaattttgcacaaaaatattcgttgtttccttgaattttaataaaagattaaatctttcacgtagtaaaaaaattccttcctcaaaatagaagttatcgtaagt Mask Tandem Repeat Region ================================================ acttacgataacttctattttgaggaaggaatttttttactacgtgaaagatttaatcttttattaaaattcaaggaaacaacgaatatttttgtgcaaaatttgttaccattagccatctattttatttgaggtaacaaatc Find is-nt database================================================ Query_seq: AA01429:AA01431|AA01429:AA01431:conserved hypothetical protein:conserved hypothetical protein (possible hydrolase):->->:959853..959995 143 acttacgataacttctattttgaggaaggaatttttttactacgtgaaagatttaatcttttattaaaattcaaggaaacaacgaatatttttgtgcaaaatttgttaccattagccatctattttatttgaggtaacaaatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01429:AA01431|AA01429:AA01431:conserved hypothetical protein:conserved hypothetical protein (possible hydrolase):->->:959853..959995 143 acttacgataacttctattttgaggaaggaatttttttactacgtgaaagatttaatcttttattaaaattcaaggaaacaacgaatatttttgtgcaaaatttgttaccattagccatctattttatttgaggtaacaaatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01429:AA01431|AA01429:AA01431:conserved hypothetical protein:conserved hypothetical protein (possible hydrolase):->->:959853..959995 143 acttacgataacttctattttgaggaaggaatttttttactacgtgaaagatttaatcttttattaaaattcaaggaaacaacgaatatttttgtgcaaaatttgttaccattagccatctattttatttgaggtaacaaatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 acttacgataacttctattttgaggaaggaatttttttactacgtgaaagatttaatcttttattaaaattcaaggaaacaacgaatatttttgtgcaaaatttgttaccattagccatctattttatttgaggtaacaaatc Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 31 End: 129 .............................. M F L L R E R F N L L L K F K E T T N I F V Q N L L P L A I Y F I .............. Protein_Len: 34 Strand: - Start: 18 End: 119 ................. K S S P I K K S R S L N L R K N F N L S V V F I K T C F K N G N A M ........................ acttacgataacttctattttgaggaaggaatttttttactacgtgaaagatttaatcttttattaaaattcaaggaaacaacgaatatttttgtgcaaaatttgttaccattagccatctattttatttgaggtaacaaatc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acttacgataacttctattttgaggaaggaatttttttactacgtgaaagatttaatcttttattaaaattcaaggaaacaacgaatatttttgtgcaaaatttgttaccattagccatctattttatttgaggtaacaaatc Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 43 HP_score: -2.4 Tail_Score: -3.39018 Start: 52 End: 68 Full_Region: gttgtttccttgaat tttaat aaaag attaaa tctttcacgtagtaa ...................................................tttaataaaagattaaa........................................................................... Find igs database================================================ Query_seq: AA01429:AA01431|AA01429:AA01431:conserved hypothetical protein:conserved hypothetical protein (possible hydrolase):->->:959853..959995 143 acttacgataacttctattttgaggaaggaatttttttactacgtgaaagatttaatcttttattaaaattcaaggaaacaacgaatatttttgtgcaaaatttgttaccattagccatctattttatttgaggtaacaaatc Intra-Species Hit: Count: 1 Min: 1 Max: 143 Len: 143 Subject: aact_AA01429_AA01431|conserved hypothetical protein:conserved hypothetical protein (possible hydrolase)|POSITIVE:POSITIVE|[959853,959995]|143 HSP 1 e-value: 3.0E-63 bit: 242.0 Len: 143 Query Start:1 Query End:143 Subject Strand: POSITIVE Subject Start: 1 Subject End: 143 acttacgataacttctattttgaggaaggaannnnnnnactacgtgaaagatttaatcttttattaaaattcaaggaaacaacgaatatttttgtgcaaaatttgttaccattagccatctattttatttgaggtaacaaatc acttacgataacttctattttgaggaaggaatttttttactacgtgaaagatttaatcttttattaaaattcaaggaaacaacgaatatttttgtgcaaaatttgttaccattagccatctattttatttgaggtaacaaatc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACUUACGAUAACUUCUAUUUUGAGGAAGGAAUUUUUUUACUACGUGAAAGAUUUAAUCUUUUAUUAAAAUUCAAGGAAACAACGAAUAUUUUUGUGCAAAAUUUGUUACCAUUAGCCAUCUAUUUUAUUUGAGGUAACAAAUC ...........(((..(((((((.((((((..((((((((...)))))))).....)))))).)))))))..)))......(((((....))))).....((((((((((.((((.............)))))))))))))). (-23.92) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUUUGUUACCUCAAAUAAAAUAGAUGGCUAAUGGUAACAAAUUUUGCACAAAAAUAUUCGUUGUUUCCUUGAAUUUUAAUAAAAGAUUAAAUCUUUCACGUAGUAAAAAAAUUCCUUCCUCAAAAUAGAAGUUAUCGUAAGU .(((((......)))))..........(((.((((((((...((((((((..(((((.....)))))...((((.((((((.....))))))...)))).)).))))))...........((......)).)))))))).))) (-17.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 242 97 57 139 100 aact:AA01922|5end_translation_initiation_factor_IF2_1294710..1294940_POSITIVE 230 117 71 201 153 aact:AA00321|5end_hypothetical_protein_219054..219284_POSITIVE 225 138 91 158 118 aact:AA01448|5end_hypothetical_protein_970208..970438_POSITIVE 225 138 91 101 61 aact:AA01446|5end_conserved_hypothetical_protein_970151..970381_POSITIVE 221 120 80 176 132 aact:AA02362|5end_conserved_hypothetical_protein_1619905..1620135_POSITIVE 215 110 61 196 154 aact:AA02615|5end_conserved_hypothetical_protein_1823040..1823270_POSITIVE 210 115 71 70 21 aact:AA02230|5end_sugar_kinase_1515984..1516214_POSITIVE 207 70 27 111 62 aact:AA01514|5end_peroxiredoxin_2_family_protein/glutaredoxin_1019890..1020120_POSITIVE 201 51 11 162 120 aact:AA02281|5end_phosphoglycerate_transport_system_activator_protein_1552843..1553073_POSITIVE 194 49 10 120 82 aact:AA00085|5end_menaquinone-specific_isochorismate;_isochorismate_mutase_62142..62372_POSITIVE 193 133 104 151 121 aact:AA02430|5end_conserved_hypothetical_protein_1675251..1675481_POSITIVE 193 117 93 100 74 aact:AA02076|5end_conserved_hypothetical_protein_1409926..1410156_POSITIVE 187 70 21 96 52 aact:AA02907|5end_thiol:disulfide_interchange_protein;_inner_membrane_copper_tolerance_protein_2033477..2033707_POSITIVE 187 125 83 222 181 aact:AA02674|5end_formate_dehydrogenase-related_protein_1859960..1860190_POSITIVE 187 120 74 226 179 aact:AA01520|5end_hypothetical_protein_1022427..1022657_POSITIVE 186 119 83 37 4 aact:AA02683|5end_hydrogenase_4_Fe-S_subunit_1868882..1869112_POSITIVE 186 119 71 211 148 aact:AA00354|5end_hypothetical_protein_234690..234920_POSITIVE 185 48 7 82 44 aact:AA01914|5end_conserved_hypothetical_protein_1290126..1290356_POSITIVE 183 62 28 203 160 aact:AA02723|5end_hypothetical_protein_1906263..1906493_POSITIVE 183 118 72 175 120 aact:AA01630|5end_threonine_efflux_protein_1102381..1102611_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 242 97 57 120 81 aact:AA01920|3end_N_utilization_substance_protein_A_1294771..1294921_POSITIVE 232 120 74 117 81 aact:AA00578|3end_DNA_topoisomerase_395201..395351_POSITIVE 230 117 71 116 68 aact:AA00318|3end_hypothetical_protein_219049..219199_POSITIVE 221 120 80 138 94 aact:AA02360|3end_threonine_synthase_1619947..1620097_POSITIVE 215 110 61 108 66 aact:AA02614|3end_conserved_hypothetical_protein_1823032..1823182_POSITIVE 202 117 79 135 104 aact:AA01477|3end_type_I_restriction_enzyme_R_protein_994479..994629_POSITIVE 193 117 93 73 47 aact:AA02075|3end_conserved_hypothetical_protein_1409979..1410129_POSITIVE 186 119 71 109 46 aact:AA00353|3end_hypothetical_protein_234668..234818_POSITIVE 185 117 72 90 51 aact:AA02959|3end_pyrroline-5-carboxylate_(P5C)_reductase_2070976..2071126_POSITIVE 185 48 7 47 9 aact:AA01913|3end_GMP_synthase_1290171..1290321_POSITIVE 184 135 92 105 65 aact:AA00144|3end_possible_carbohydrate_diacid_regulator_104986..105136_POSITIVE 181 118 96 148 128 aact:AA02480|3end_excinuclease_ABC_subunit_B_1718442..1718592_POSITIVE 180 136 106 56 26 aact:AA01053|3end_conserved_hypothetical_protein_733747..733897_POSITIVE 179 119 88 116 85 aact:AA02939|3end_phosphoserine_phosphatase_2057902..2058052_POSITIVE 179 126 88 65 31 aact:AA02249|3end_thiamine_biosynthesis_protein_1529899..1530049_POSITIVE 179 84 42 108 69 aact:AA00872|3end_rough_colony_protein_B_604520..604670_POSITIVE 177 120 80 46 8 aact:AA00992|3end_ribonuclease_BN_694568..694718_POSITIVE 176 122 83 143 103 aact:AA00448|3end_C-terminal_region_of_competence_protein_M_313058..313208_POSITIVE 174 117 73 148 94 aact:AA00820|3end_succinyl-diaminopimelate_desuccinylase_564536..564686_POSITIVE 173 134 112 82 55 aact:AA01801|3end_malate_dehydrogenase_1214370..1214520_POSITIVE