Origin IGS: taagggataaaagtgcggtcgtttctacaggcgttttttaacacaaaaaatagcgataggatttgctgtcggtgacgtcctgataaacgtagcccagttgttctaacgcctgttcaagttcggcaccgttgccttgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacaaggcaacggtgccgaacttgaacaggcgttagaacaactgggctacgtttatcaggacgtcaccgacagcaaatcctatcgctattttttgtgttaaaaaacgcctgtagaaacgaccgcacttttatccctta Mask Tandem Repeat Region ================================================ taagggataaaagtgcggtcgtttctacaggcgttttttaacacaaaaaatagcgataggatttgctgtcggtgacgtcctgataaacgtagcccagttgttctaacgcctgttcaagttcggcaccgttgccttgtta Find is-nt database================================================ Query_seq: AA01423:AA01424|AA01423:AA01424:cell division protein X, ABC system permease:threonine deaminase (dehydratase):->->:957741..957879 139 taagggataaaagtgcggtcgtttctacaggcgttttttaacacaaaaaatagcgataggatttgctgtcggtgacgtcctgataaacgtagcccagttgttctaacgcctgttcaagttcggcaccgttgccttgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01423:AA01424|AA01423:AA01424:cell division protein X, ABC system permease:threonine deaminase (dehydratase):->->:957741..957879 139 taagggataaaagtgcggtcgtttctacaggcgttttttaacacaaaaaatagcgataggatttgctgtcggtgacgtcctgataaacgtagcccagttgttctaacgcctgttcaagttcggcaccgttgccttgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01423:AA01424|AA01423:AA01424:cell division protein X, ABC system permease:threonine deaminase (dehydratase):->->:957741..957879 139 taagggataaaagtgcggtcgtttctacaggcgttttttaacacaaaaaatagcgataggatttgctgtcggtgacgtcctgataaacgtagcccagttgttctaacgcctgttcaagttcggcaccgttgccttgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagggataaaagtgcggtcgtttctacaggcgttttttaacacaaaaaatagcgataggatttgctgtcggtgacgtcctgataaacgtagcccagttgttctaacgcctgttcaagttcggcaccgttgccttgtta Predict ORF larger than 30AA ================================================ taagggataaaagtgcggtcgtttctacaggcgttttttaacacaaaaaatagcgataggatttgctgtcggtgacgtcctgataaacgtagcccagttgttctaacgcctgttcaagttcggcaccgttgccttgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagggataaaagtgcggtcgtttctacaggcgttttttaacacaaaaaatagcgataggatttgctgtcggtgacgtcctgataaacgtagcccagttgttctaacgcctgttcaagttcggcaccgttgccttgtta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01423:AA01424|AA01423:AA01424:cell division protein X, ABC system permease:threonine deaminase (dehydratase):->->:957741..957879 139 taagggataaaagtgcggtcgtttctacaggcgttttttaacacaaaaaatagcgataggatttgctgtcggtgacgtcctgataaacgtagcccagttgttctaacgcctgttcaagttcggcaccgttgccttgtta Intra-Species Hit: Count: 1 Min: 1 Max: 139 Len: 139 Subject: aact_AA01423_AA01424|cell division protein X, ABC system permease:threonine deaminase (dehydratase)|POSITIVE:POSITIVE|[957741,957879]|139 HSP 1 e-value: 2.0E-73 bit: 276.0 Len: 139 Query Start:1 Query End:139 Subject Strand: POSITIVE Subject Start: 1 Subject End: 139 taagggataaaagtgcggtcgtttctacaggcgttttttaacacaaaaaatagcgataggatttgctgtcggtgacgtcctgataaacgtagcccagttgttctaacgcctgttcaagttcggcaccgttgccttgtta taagggataaaagtgcggtcgtttctacaggcgttttttaacacaaaaaatagcgataggatttgctgtcggtgacgtcctgataaacgtagcccagttgttctaacgcctgttcaagttcggcaccgttgccttgtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGGGAUAAAAGUGCGGUCGUUUCUACAGGCGUUUUUUAACACAAAAAAUAGCGAUAGGAUUUGCUGUCGGUGACGUCCUGAUAAACGUAGCCCAGUUGUUCUAACGCCUGUUCAAGUUCGGCACCGUUGCCUUGUUA ..((((.(((..((((.(...(((..(((((((((...((((.......(((((((......))))))).(((.((((........)))).)))..))))....)))))))))..)))..).))))..))))))).... (-42.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACAAGGCAACGGUGCCGAACUUGAACAGGCGUUAGAACAACUGGGCUACGUUUAUCAGGACGUCACCGACAGCAAAUCCUAUCGCUAUUUUUUGUGUUAAAAAACGCCUGUAGAAACGACCGCACUUUUAUCCCUUA ....((((.((.(((((.........(((((((((..((((....((..((((((....))))))..))...(((..........)))........))))....)))))))))..........))))).))...)))). (-32.31) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 437 40 1 139 101 aact:AA00819|5end_D-alanyl-D-alanine_carboxypeptidase_562644..562874_POSITIVE 418 44 1 53 11 aact:AA02484|5end_ABC_transporter,_ATP-binding_protein_1721077..1721307_POSITIVE 397 58 11 216 167 aact:AA01344|5end_magnesium_and_cobalt_transport_protein_911661..911891_POSITIVE 396 38 2 122 84 aact:AA00860|5end_phosphoheptose_isomerase_591866..592096_POSITIVE 389 48 4 151 101 aact:AA00378|5end_leucine-responsive_regulatory_protein_(transcription_regulator)_254324..254554_POSITIVE 375 120 71 184 141 aact:AA02506|5end_possible_transmembrane_protein_1739287..1739517_POSITIVE 375 42 8 186 152 aact:AA01173|5end_VapD-homolog_803850..804080_POSITIVE 371 45 1 66 23 aact:AA00602|5end_1-deoxy-D-xylulose_5-phosphate_reductoisomerase_409077..409307_POSITIVE 369 126 87 230 178 aact:AA02043|5end_tagatose_6-phosphate_kinase_1390218..1390448_POSITIVE 368 100 51 132 79 aact:AA01274|5end_hypothetical_protein_864519..864749_POSITIVE 367 42 3 134 93 aact:AA02288|5end_conserved_hypothetical_protein_1558590..1558820_POSITIVE 361 99 53 186 140 aact:AA02351|5end_conserved_hypothetical_protein_1609233..1609463_POSITIVE 359 38 7 226 195 aact:AA01598|5end_conserved_hypothetical_protein_1078954..1079184_POSITIVE 356 114 79 121 77 aact:AA00603|5end_undecaprenyl_pyrophosphate_synthetase_(UPPS)_410283..410513_POSITIVE 350 41 7 145 111 aact:AA00766|5end_heat_shock_protein_B25.3_(HSP-70_cofactor)_527384..527614_POSITIVE 349 100 53 47 1 aact:AA01019|5end_conserved_hypothetical_protein_712223..712453_POSITIVE 349 43 3 176 141 aact:AA00569|5end_ketol-acid_reductoisomerase_391191..391421_POSITIVE 346 37 2 171 138 aact:AA03003|5end_GTP-binding_membrane_protein_2096544..2096774_POSITIVE 346 37 2 50 17 aact:AA03002|5end_hypothetical_protein_2096423..2096653_POSITIVE 346 46 10 181 145 aact:AA00075|5end_conserved_hypothetical_protein_54963..55193_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 375 120 71 65 22 aact:AA02504|3end_lipid_A_biosynthesis_(kdo)2-(lauroyl)-lipid_IVA_acyltransferase_1739248..1739398_POSITIVE 375 42 8 103 69 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 368 100 51 121 68 aact:AA01273|3end_hypothetical_protein_864588..864738_POSITIVE 361 99 53 52 6 aact:AA02350|3end_possible_lipoprotein_1609179..1609329_POSITIVE 359 38 7 128 97 aact:AA01597|3end_oligoribonuclease_1078936..1079086_POSITIVE 356 48 9 47 5 aact:AA00377|3end_acetyl-CoA_acetyltransferase_(Beta-ketothiolase,3-ketoacyl-CoA_thiolase)_254300..254450_POSITIVE 346 37 2 34 1 aact:AA03001|3end_probable_glycyl_radical_protein/pyruvate-formate_lyase_2096487..2096637_POSITIVE 346 46 10 85 49 aact:AA00072|3end_conserved_hypothetical_protein_54947..55097_POSITIVE 342 44 2 93 53 aact:AA00684|3end_adenylosuccinate_synthase_468861..469011_POSITIVE 340 41 9 149 117 aact:AA01646|3end_hypothetical_protein_1115262..1115412_POSITIVE 340 41 9 71 39 aact:AA01645|3end_ferric_transport_ABC-related_ATP-binding_protein_1115184..1115334_POSITIVE 337 42 9 78 45 aact:AA02280|3end_conserved_hypothetical_protein_1552767..1552917_POSITIVE 334 40 7 149 115 aact:AA02507|3end_penicillin-insensitive_murein_endopeptidase_A_1741007..1741157_POSITIVE 329 100 58 44 1 aact:AA01018|3end_conserved_hypothetical_protein_(possible_transcriptional_regulator)_712300..712450_POSITIVE 328 47 8 68 27 aact:AA02412|3end_cysteine_synthetase_1658552..1658702_POSITIVE 328 96 51 132 93 aact:AA01995|3end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355394..1355544_POSITIVE 325 52 11 50 8 aact:AA02446|3end_conserved_hypothetical_protein_1687583..1687733_POSITIVE 322 47 7 100 58 aact:AA00508|3end_SAM-dependent_methyltransferase_353883..354033_POSITIVE 320 38 3 128 97 aact:AA01384|3end_hypothetical_protein_935540..935690_POSITIVE 319 39 6 131 99 aact:AA01434|3end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_963021..963171_POSITIVE