Origin IGS: taatggatagtctgttttgactgtaattatcattttatcaacgttatgattctcgccttcccctgtttgcggggagggggacgtaccacgttttgagcgcctgctctgccggcgaccccgaagaggatagcaagtaaagaatgaacttgcgaataacttgtcccgaagagactgaaaggaaaatacctaaattttcgaccgcactttccccccctcagccttcggcagctcaccccgtaaacgggagggagccaagattgacacaacatattagagaaaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttttttctctaatatgttgtgtcaatcttggctccctcccgtttacggggtgagctgccgaaggctgagggggggaaagtgcggtcgaaaatttaggtattttcctttcagtctcttcgggacaagttattcgcaagttcattctttacttgctatcctcttcggggtcgccggcagagcaggcgctcaaaacgtggtacgtccccctccccgcaaacaggggaaggcgagaatcataacgttgataaaatgataattacagtcaaaacagactatccatta Mask Tandem Repeat Region ================================================ taatggatagtctgttttgactgtaattatcattttatcaacgttatgattctcgccttcccctgtttgcggggagggggacgtaccacgttttgagcgcctgctctgccggcgaccccgaagaggatagcaagtaaagaatgaacttgcgaataacttgtcccgaagagactgaaaggaaaatacctaaattttcgaccgcactttccccccctcagccttcggcagctcaccccgtaaacgggagggagccaagattgacacaacatattagagaaaaaa Find is-nt database================================================ Query_seq: AA01421:AA01422|AA01421:AA01422:phosphoglycolate phosphatase:cell division protein E, ABC system ATP-binding protein:->->:955867..956148 282 taatggatagtctgttttgactgtaattatcattttatcaacgttatgattctcgccttcccctgtttgcggggagggggacgtaccacgttttgagcgcctgctctgccggcgaccccgaagaggatagcaagtaaagaatgaacttgcgaataacttgtcccgaagagactgaaaggaaaatacctaaattttcgaccgcactttccccccctcagccttcggcagctcaccccgtaaacgggagggagccaagattgacacaacatattagagaaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01421:AA01422|AA01421:AA01422:phosphoglycolate phosphatase:cell division protein E, ABC system ATP-binding protein:->->:955867..956148 282 taatggatagtctgttttgactgtaattatcattttatcaacgttatgattctcgccttcccctgtttgcggggagggggacgtaccacgttttgagcgcctgctctgccggcgaccccgaagaggatagcaagtaaagaatgaacttgcgaataacttgtcccgaagagactgaaaggaaaatacctaaattttcgaccgcactttccccccctcagccttcggcagctcaccccgtaaacgggagggagccaagattgacacaacatattagagaaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01421:AA01422|AA01421:AA01422:phosphoglycolate phosphatase:cell division protein E, ABC system ATP-binding protein:->->:955867..956148 282 taatggatagtctgttttgactgtaattatcattttatcaacgttatgattctcgccttcccctgtttgcggggagggggacgtaccacgttttgagcgcctgctctgccggcgaccccgaagaggatagcaagtaaagaatgaacttgcgaataacttgtcccgaagagactgaaaggaaaatacctaaattttcgaccgcactttccccccctcagccttcggcagctcaccccgtaaacgggagggagccaagattgacacaacatattagagaaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatggatagtctgttttgactgtaattatcattttatcaacgttatgattctcgccttcccctgtttgcggggagggggacgtaccacgttttgagcgcctgctctgccggcgaccccgaagaggatagcaagtaaagaatgaacttgcgaataacttgtcccgaagagactgaaaggaaaatacctaaattttcgaccgcactttccccccctcagccttcggcagctcaccccgtaaacgggagggagccaagattgacacaacatattagagaaaaaa Predict ORF larger than 30AA ================================================ Protein_Len: 47 Strand: + Start: 141 End: 281 ............................................................................................................................................ M N L R I T C P E E T E R K I P K F S T A L S P P Q P S A A H P V N G R E P R L T Q H I R E K . Protein_Len: 46 Strand: - Start: 47 End: 184 .............................................. S E R R G R N A P L P V Y W T K L A Q E A P S G S S S L L Y L I F K R I V Q G S S V S L F M .................................................................................................. Protein_Len: 60 Strand: - Start: 33 End: 269 ................................ N Q A G A R G A V G F L I A L L S H V Q S Y S T G F L S F P F Y R F K R G C K G G R L R R C S V G Y V P P L W S Q C L M ............. taatggatagtctgttttgactgtaattatcattttatcaacgttatgattctcgccttcccctgtttgcggggagggggacgtaccacgttttgagcgcctgctctgccggcgaccccgaagaggatagcaagtaaagaatgaacttgcgaataacttgtcccgaagagactgaaaggaaaatacctaaattttcgaccgcactttccccccctcagccttcggcagctcaccccgtaaacgggagggagccaagattgacacaacatattagagaaaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatggatagtctgttttgactgtaattatcattttatcaacgttatgattctcgccttcccctgtttgcggggagggggacgtaccacgttttgagcgcctgctctgccggcgaccccgaagaggatagcaagtaaagaatgaacttgcgaataacttgtcccgaagagactgaaaggaaaatacctaaattttcgaccgcactttccccccctcagccttcggcagctcaccccgtaaacgggagggagccaagattgacacaacatattagagaaaaaa Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 41 HP_score: -2.6 Tail_Score: -2.91728 Start: 130 End: 150 Full_Region: cgggacaagttattc gcaagt tcattcttt acttgc tatcctcttcggggt .................................................................................................................................gcaagttcattctttacttgc.................................................................................................................................... TransTerm Strand: - Conf: 41 HP_score: -2.8 Tail_Score: -2.85583 Start: 179 End: 196 Full_Region: ggggaaagtgcggtc gaaaat ttaggt attttc ctttcagtctcttcg ..................................................................................................................................................................................gaaaatttaggtattttc...................................................................................... Find igs database================================================ Query_seq: AA01421:AA01422|AA01421:AA01422:phosphoglycolate phosphatase:cell division protein E, ABC system ATP-binding protein:->->:955867..956148 282 taatggatagtctgttttgactgtaattatcattttatcaacgttatgattctcgccttcccctgtttgcggggagggggacgtaccacgttttgagcgcctgctctgccggcgaccccgaagaggatagcaagtaaagaatgaacttgcgaataacttgtcccgaagagactgaaaggaaaatacctaaattttcgaccgcactttccccccctcagccttcggcagctcaccccgtaaacgggagggagccaagattgacacaacatattagagaaaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 282 Len: 282 Subject: aact_AA01421_AA01422|phosphoglycolate phosphatase:cell division protein E, ABC system ATP-binding protein|POSITIVE:POSITIVE|[955867,956148]|282 HSP 1 e-value: 1.0E-146 bit: 517.0 Len: 282 Query Start:1 Query End:282 Subject Strand: POSITIVE Subject Start: 1 Subject End: 282 taatggatagtctgttttgactgtaattatcattttatcaacgttatgattctcgccttcccctgtttgcggggagggggacgtaccacgttttgagcgcctgctctgccggcgaccccgaagaggatagcaagtaaagaatgaacttgcgaataacttgtcccgaagagactgaaaggaaaatacctaaattttcgaccgcactttnnnnnnntcagccttcggcagctcaccccgtaaacgggagggagccaagattgacacaacatattagagaaaaaa taatggatagtctgttttgactgtaattatcattttatcaacgttatgattctcgccttcccctgtttgcggggagggggacgtaccacgttttgagcgcctgctctgccggcgaccccgaagaggatagcaagtaaagaatgaacttgcgaataacttgtcccgaagagactgaaaggaaaatacctaaattttcgaccgcactttccccccctcagccttcggcagctcaccccgtaaacgggagggagccaagattgacacaacatattagagaaaaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGGAUAGUCUGUUUUGACUGUAAUUAUCAUUUUAUCAACGUUAUGAUUCUCGCCUUCCCCUGUUUGCGGGGAGGGGGACGUACCACGUUUUGAGCGCCUGCUCUGCCGGCGACCCCGAAGAGGAUAGCAAGUAAAGAAUGAACUUGCGAAUAACUUGUCCCGAAGAGACUGAAAGGAAAAUACCUAAAUUUUCGACCGCACUUUCCCCCCCUCAGCCUUCGGCAGCUCACCCCGUAAACGGGAGGGAGCCAAGAUUGACACAACAUAUUAGAGAAAAAA ((((..((((((......)))))).)))).............((((....(((.(.((((((((((((((((((.((((((((.....))......(((....((((..(((.(((.((.....))...((((((.........)))))).........)))))).))))........((((((......))))))...)))....)))))).....(((...)))......))))))))))))).))))).).))).)))).................... (-85.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUUUCUCUAAUAUGUUGUGUCAAUCUUGGCUCCCUCCCGUUUACGGGGUGAGCUGCCGAAGGCUGAGGGGGGGAAAGUGCGGUCGAAAAUUUAGGUAUUUUCCUUUCAGUCUCUUCGGGACAAGUUAUUCGCAAGUUCAUUCUUUACUUGCUAUCCUCUUCGGGGUCGCCGGCAGAGCAGGCGCUCAAAACGUGGUACGUCCCCCUCCCCGCAAACAGGGGAAGGCGAGAAUCAUAACGUUGAUAAAAUGAUAAUUACAGUCAAAACAGACUAUCCAUUA ..((((.((....((((((((....(((((.((.((.((.((((.(((((.(.((((.(((...((((((((((((((((((..............))))))))).....(((.(....)))).........((((((.........))))))...)))))))))..))).)))).((((....))))...(((.....)))....).))))).)))).)))).)).)))))..)))))))).)).))))..........((((......))))........ (-88.44) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 601 139 92 231 188 aact:AA00200|5end_thymidylate_synthase_138892..139122_POSITIVE 541 99 51 218 170 aact:AA01174|5end_hypothetical_protein_804700..804930_POSITIVE 462 257 211 231 183 aact:AA02949|5end_hypothetical_protein_2061944..2062174_POSITIVE 441 126 86 187 144 aact:AA02108|5end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1433393..1433623_POSITIVE 438 120 72 185 117 aact:AA01400|5end_phosphopantetheine_adenylyltransferase_945679..945909_POSITIVE 428 120 71 176 129 aact:AA00729|5end_possible_hemolysin_(TlyC-like)_500204..500434_POSITIVE 421 236 195 183 139 aact:AA01648|5end_alpha-amylase_protein;_1,4-alpha-D-glucan_glucanohydrolase_1115170..1115400_POSITIVE 421 236 195 82 38 aact:AA01646|5end_hypothetical_protein_1115069..1115299_POSITIVE 421 108 64 131 82 aact:AA00957|5end_porphobilinogen_deaminase_(hydroxymethylbilane_synthase)_661006..661236_POSITIVE 421 110 61 62 15 aact:AA00581|5end_thiosulfate_sulfurtransferase_(rhodanese)_397605..397835_POSITIVE 413 127 81 85 18 aact:AA01224|5end_CTP_synthase_836221..836451_POSITIVE 412 134 92 44 1 aact:AA02106|5end_hypothetical_protein_1433241..1433471_POSITIVE 408 269 221 173 125 aact:AA02199|5end_hypothetical_protein_1497627..1497857_POSITIVE 404 109 61 216 145 aact:AA01379|5end_glycerophosphoryl_diester_phosphodiesterase_(protein_D)_933029..933259_POSITIVE 395 140 95 54 19 aact:AA01637|5end_ribonucleoside_diphosphate_reductase_1106657..1106887_POSITIVE 391 100 51 215 149 aact:AA01146|5end_conserved_hypothetical_protein_784433..784663_POSITIVE 388 236 196 95 50 aact:AA02091|5end_hypothetical_protein_1424441..1424671_POSITIVE 388 120 71 166 118 aact:AA00799|5end_iron(III)_dicitrate_transport_ATP-binding_protein_547658..547888_POSITIVE 387 250 201 77 13 aact:AA01845|5end_gamma-glutamyltranspeptidase_precursor_1239841..1240071_POSITIVE 387 138 91 162 119 aact:AA01633|5end_probable_ferredoxin-like_protein_1104008..1104238_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 462 257 211 81 33 aact:AA02947|3end_riboflavin_biosynthesis_protein_2061874..2062024_POSITIVE 438 120 72 117 49 aact:AA01398|3end_KDO_kinase_945691..945841_POSITIVE 421 226 184 126 66 aact:AA02564|3end_transketolase_N-terminal_section_1786783..1786933_POSITIVE 421 236 195 117 73 aact:AA01645|3end_ferric_transport_ABC-related_ATP-binding_protein_1115184..1115334_POSITIVE 421 108 64 115 66 aact:AA00954|3end_uroporphyrinogen_III_synthase_661070..661220_POSITIVE 398 125 91 135 102 aact:AA02864|3end_glycerol-3-phosphate_transport_protein_2010192..2010342_POSITIVE 388 236 196 123 78 aact:AA02090|3end_glucose-1-phosphate_adenylyltransferase_1424549..1424699_POSITIVE 383 264 221 149 110 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 373 255 214 77 29 aact:AA00239|3end_hypothetical_protein_171418..171568_POSITIVE 371 78 31 149 102 aact:AA02351|3end_conserved_hypothetical_protein_1609468..1609618_POSITIVE 371 120 72 120 46 aact:AA01843|3end_sensor_protein;_probable_two-component_system_sensor_histidine_kinase_1239830..1239980_POSITIVE 366 248 202 131 72 aact:AA01566|3end_conserved_hypothetical_protein_1052091..1052241_POSITIVE 366 126 88 51 14 aact:AA01342|3end_conserved_hypotheticl_protein_(possible_ribosomal_RNA_adenine_dimethylase)_911085..911235_POSITIVE 366 248 202 131 72 aact:AA01261|3end_conserved_hypothetical_protein_856723..856873_POSITIVE 365 128 81 69 18 aact:AA02369|3end_conserved_hypothetical_protein_1624648..1624798_POSITIVE 364 118 79 133 93 aact:AA01254|3end_conserved_hypothetical_protein_(possible_proline_dipeptidase)_852702..852852_POSITIVE 360 116 72 46 8 aact:AA00525|3end_hypothetical_protein_359459..359609_POSITIVE 358 207 161 60 5 aact:AA01289|3end_hypothetical_protein_872848..872998_POSITIVE 358 114 76 48 16 aact:AA00534|3end_hypothetical_protein_365594..365744_POSITIVE 356 128 82 87 22 aact:AA00933|3end_ribose_ABC_transporter,_ATP-binding_protein_645197..645347_POSITIVE